Dictyostelium discoideum protocols
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2013
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| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
983 |
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| Online-Zugang: | Inhaltsverzeichnis |
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| 650 | 2 | |a Dictyostelium |a genetics | |
| 650 | 2 | |a Genetic Techniques | |
| 650 | 2 | |a Models, Biological | |
| 650 | 2 | |a Models, Genetic | |
| 650 | 0 | 7 | |a Dictyostelium discoideum |0 (DE-588)4012135-5 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1819583582074568704 |
|---|---|
| adam_text | Dictyostelium discoideum
Protocols
Second Edition
Edited by
Ludwig Eichinger
Centre for Biochemistry, Medical Faculty, University of Cologne, Koln, Germany
Francisco Rivero
Centre for Cardiovascular and Metabolic Research, The Hull York Medical School,
University of Hull, Hull, UK
Humana Press
o
Contents
Preface v
Contributors ix
1 The Amoebozoa 1
Christina Schilde and Pauline Schaap
2 The Model Organism Dictyostelium discoideum 17
Salvatore Bozzaro
3 Comparative Genomics of the Dictyostelids 39
William F Loomis
4 One Stop Shop for Everything Dictyostelium- ilictyBase
and the Dicty Stock Center in 2012 59
Petra Fey, Robert J Dodson, Siddhartha Basu, and Rex L Chisholm
5 Fluorescent Reporters and Methods to Analyze Fluorescent Signals 93
Annette Miiller-Taubenberger and Hellen C Ishikawa-Ankerhold
6 Collection and Cultivation of Dictyostelids from the Wild 113
Tracy E Douglas, Debra A Brock, Boahemaa Adu-Oppong,
David C Queller, and Joan E Strassmann
7 Identification and Verification of microRNAs
by High-Throughput Sequencing 125
Jimmie Hdllman, Lotta Avesson, Johan Reimegard,
Max Kaller, and Fredrik Soderbom
8 Transcriptional Profiling of Dictyostelium with RNA Sequencing 139
Edward Roshan Miranda, Gregor Rot, Marko Toplak,
Balaji Santhanam, Tomaz Curk, Gad Shaulsky, and Blaz Zupan
9 Analysis of Chromatin Organization by Deep Sequencing Technologies 173
James L Piatt, Nick A Kent, Adrian J Harwood, and Alan R Kimmel
10 Pharmacogenetics of Resistance to Cisplatin and Other Anticancer
Drugs and the Role of Sphingolipid Metabolism 185
Stephen Alexander, William S Swatson, and Hannah Alexander
11 N-Glvcomic and N-Glvcoproteomic Studies in the Social Amoebae 205
Christa L Feasley, Alba Hykollari, Katharina Paschinger,
Iain B H Wilson, and Christopher M West
12 Measuring Cheating, Fitness, and Segregation in Dictyostelium discoideum 231
Neil J Buttery, Jeff Smith, David C Queller,
and Joan E Strassmann
13 The Application of the Crc-loxP System for Generating Multiple
Knock-out and Knock-in Targeted Loci 249
Jan Faix, Joern Linkner, Benjamin Nordholz, James L Piatt,
Xin-Hua Liao, and Alan R Kimmel
vii
viii Contents
14 Extrachromosomal Inducible Expression 269
Douwe M Veltman and Peter J M Van Haastert ®
15 Isolation of Dictyostelium Nuclei for Light and Electron Microscopy 283
Petros Batsios, Otto Baumann, Ralph Graf, and Irene Meyer
16 Investigation of DNA Repair Pathway Activity 295
Anne-Marie C Couto, Nicholas D Lakin, and Catherine J Pears
17 Transcript Localization in Dictyostelium discoideum Cells by RNA FISH 311
Patrick Hofmann, Janis Kruse, and Christian Hammann
18 Analysis of Mitochondrial Gene Expression 325
Jessica E Accari, Sam Manna, Paul R Fisher, and Christian Barth
19 Mitochondrial Respiratory Complex Function and the Phenotypic
Consequences of Dysfunction 345
Sarah J Annesley, Sergio Carilla-Latorre, Ricardo Escalante,
and Paul R Fisher
20 Micropipette Aspiration for Studying Cellular Mechanosensory
Responses and Mechanics 367
Yee-Seir Kee and Douglas N Robinson
21 Quantitative Analysis of Phagocytosis and Phagosome Maturation 383
Natascha Sattler, Roger Monroy, and Thierry Soldati
22 Setting Up and Monitoring an Infection of Dictyostelium
discoideum with Mycobacteria 403
Sonia Arafah, Sebastien Kicka, Valentin Trofimov,
Monica Hagedorn, Nuria Andreu, Siouxsie Wiles, Brian Robertson,
and Thierry Soldati
23 Isolation of Pathogen-Containing Vacuoles 419
Olga Shevchuk and Michael Steinert
24 Immunomagnetic Purification of Fluorescent
Legionella-Containing Vacuoles 431
Ivo Finsel, Christine Hoffmann, and Hubert Hilbi
25 Secretory Lysosomes in Dictyostelium- Visualization,
Characterization, and Dynamics 445
Wanessa C Lima and Pierre Cosson
26 Monitoring Autophagy in Dictyostelium 461
Ana Mesquita, Javier Calvo-Garrido, Sergio Carilla-Latorre,
and Ricardo Escalante
Index 471
|
| any_adam_object | 1 |
| author2 | Eichinger, Ludwig 1958- |
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| author_GND | (DE-588)139496092 |
| author_facet | Eichinger, Ludwig 1958- |
| building | Verbundindex |
| bvnumber | BV040904046 |
| classification_rvk | WG 4180 |
| ctrlnum | (OCoLC)841753847 (DE-599)HBZHT017429938 |
| dewey-full | 579.432 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 579 - Microorganisms, fungi & algae |
| dewey-raw | 579.432 |
| dewey-search | 579.432 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 2. ed. |
| format | Book |
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| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Laboratory Manuals |
| genre_facet | Aufsatzsammlung Laboratory Manuals |
| id | DE-604.BV040904046 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T03:11:13Z |
| institution | BVB |
| isbn | 9781627033015 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025883516 |
| oclc_num | 841753847 |
| open_access_boolean | |
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| owner_facet | DE-11 DE-12 |
| physical | XII, 479 S. Ill., graph. Darst |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spellingShingle | Dictyostelium discoideum protocols Methods in molecular biology Dictyostelium cytology Dictyostelium genetics Genetic Techniques Models, Biological Models, Genetic Dictyostelium discoideum (DE-588)4012135-5 gnd |
| subject_GND | (DE-588)4012135-5 (DE-588)4143413-4 |
| title | Dictyostelium discoideum protocols |
| title_auth | Dictyostelium discoideum protocols |
| title_exact_search | Dictyostelium discoideum protocols |
| title_full | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... |
| title_fullStr | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... |
| title_full_unstemmed | Dictyostelium discoideum protocols ed. by Ludwig Eichinger ... |
| title_short | Dictyostelium discoideum protocols |
| title_sort | dictyostelium discoideum protocols |
| topic | Dictyostelium cytology Dictyostelium genetics Genetic Techniques Models, Biological Models, Genetic Dictyostelium discoideum (DE-588)4012135-5 gnd |
| topic_facet | Dictyostelium cytology Dictyostelium genetics Genetic Techniques Models, Biological Models, Genetic Dictyostelium discoideum Aufsatzsammlung Laboratory Manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025883516&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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