Mammalian oocyte regulation methods and protocols

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Veröffentlicht: New York [u.a.] Humana Press 2013
Schriftenreihe:Methods in molecular biology 957
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adam_text Mammalian Oocyte Regulation Methods and Protocols Edited by Hayden A Homer Department of Cell and Developmental Biology, Institute for Women s Health, University College London, London, UK Humana Press Contents Preface v Contributors ix 1 Studying Recombination in Mouse Oocytes 1 Xianfei Sun and Paula E Cohen 2 Analysis of Recombinants in Female Mouse Meiosis 19 Esther de Boer, Maria Jasin, and Scott Keeney 3 Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes 47 Ji-hye Kim, Kei-ichiro Ishiguro, Nobuaki Kudo, and Toshinori Watanabe 4 Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development 59 Jing He, Andrew J Childs, Jieqian Zhou, and Richard A Anderson 5 Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos 77 Jacek Z Kubiak s 6 Time-Lapse Epifluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes 91 Janet E Holt, Simon I R Lane, and Keith T Jones 7 Using FRET to Study RanGTP Gradients in Live Mouse Oocytes 107 Julien Dumont and Marie-Helene Verlhac 8 Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging in Oocytes 121 Mark Levasseur 9 RNAi-Based Methods for Gene Silencing in Mouse Oocytes 135 Paula Stein, Petr Svoboda, and Richard M Schultz 10 Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes 153 Petros Marangos 11 Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos 163 Jay M Baltz, Hannah E Corbett, and Samantha Richard 12 Immunofluorescence Staining of Spindles, Chromosomes, and Kinetochores in Human Oocytes 179 Solon Riris, Suzanne Cawood, Liming Gui, Paul Serhal, and Hayden A Homer 13 Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes 189 Kuo-Tai Tang, Ti-Nan Lin, Shu-Kuei Li, and Tang K Tang vii viii Contents 14 Chromosome Spreads with Centromere Staining in Mouse Oocytes 203 Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann 15 Preparation of Mammalian Oocytes for Transmission Electron Microscopy 213 Lynne Anguish and Scott Coonrod 16 Measuring Ca2+Oscillations in Mammalian Eggs 231 Karl Swann 17 Counting Chromosomes in Intact Eggs 249 Teresa Chiang and Michael A Lampson 18 Free-Hand Bisection of Mouse Oocytes and Embryos 255 Zbigniew Polanski and Jacek Z Kubiak 19 Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes 267 Souraya Jaroudi and Dagan Wells 20 Nuclear Transfer in the Mouse Oocyte 285 Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama Index 301
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physical XI, 311 S. Ill., graph. Darst.
publishDate 2013
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record_format marc
series Methods in molecular biology
series2 Methods in molecular biology
Springer protocols
spelling Mammalian oocyte regulation methods and protocols ed. by Hayden A. Homer
New York [u.a.] Humana Press 2013
XI, 311 S. Ill., graph. Darst.
txt rdacontent
n rdamedia
nc rdacarrier
Methods in molecular biology 957
Springer protocols
Includes bibliographical references and index
Ovum
Oogenesis
Mice / Embryology
Human embryo
Mammals / Embryology
Oocytes / Laboratory Manuals
Mice / embryology / Laboratory Manuals
Oogenesis / Laboratory Manuals
Homer, Hayden A. Sonstige oth
Erscheint auch als Online-Ausgabe 978-1-62703-191-2
Methods in molecular biology 957 (DE-604)BV035362695 957
HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025744721&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle Mammalian oocyte regulation methods and protocols
Methods in molecular biology
Ovum
Oogenesis
Mice / Embryology
Human embryo
Mammals / Embryology
Oocytes / Laboratory Manuals
Mice / embryology / Laboratory Manuals
Oogenesis / Laboratory Manuals
title Mammalian oocyte regulation methods and protocols
title_auth Mammalian oocyte regulation methods and protocols
title_exact_search Mammalian oocyte regulation methods and protocols
title_full Mammalian oocyte regulation methods and protocols ed. by Hayden A. Homer
title_fullStr Mammalian oocyte regulation methods and protocols ed. by Hayden A. Homer
title_full_unstemmed Mammalian oocyte regulation methods and protocols ed. by Hayden A. Homer
title_short Mammalian oocyte regulation
title_sort mammalian oocyte regulation methods and protocols
title_sub methods and protocols
topic Ovum
Oogenesis
Mice / Embryology
Human embryo
Mammals / Embryology
Oocytes / Laboratory Manuals
Mice / embryology / Laboratory Manuals
Oogenesis / Laboratory Manuals
topic_facet Ovum
Oogenesis
Mice / Embryology
Human embryo
Mammals / Embryology
Oocytes / Laboratory Manuals
Mice / embryology / Laboratory Manuals
Oogenesis / Laboratory Manuals
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025744721&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link (DE-604)BV035362695
work_keys_str_mv AT homerhaydena mammalianoocyteregulationmethodsandprotocols