The neurodegenerative Drosophila melanogaster AMPK mutant loechrig
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2012
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| Online-Zugang: | http://d-nb.info/1024851869/34 kostenfrei Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a The neurodegenerative Drosophila melanogaster AMPK mutant loechrig |c von Mandy Cook. Betreuer: Doris Kretzschmar |
| 246 | 1 | 3 | |a The neurodegenerative Drosophila melanogaster AMPK Mutante loechrig |
| 264 | 1 | |c 2012 | |
| 300 | |a VI, 93 S. |b Illustrationen, Graphen | ||
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Datensatz im Suchindex
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| adam_text |
IMAGE 1
TABLE OF CONTENTS
TABLE OF FIGURES V
LIST OF TABLES V I
SUMMARY 1
ZUSAMMENFASSUNG 3
1 INTRODUCTION 6
1.1 WHY USE DROSOPHILA MELANOGASTER AS MODEL ORGANISM 7
1.2 THE DROSOPHILA MUTANT LOECHRIG 9
1.3 AMPK - AND WHY IT IS SO IMPORTANT 10
1.3.1 AMPK UPSTREAM KINASES 14
1.3.2 AMPK MUTATION CAUSING DISEASE IN HUMANS 14
1.4 THE LOE PROTEIN 17
1.5 PROTEIN PRENYLATION 19
1.5.1 THE ISROPRENOID PATHWAY 20
1.6 THE RHO PATHWAY AND THE ROLE OF RHO 23
1.6.1 ACTIN 24
1.6.2 LIM-KINASE AND COFILIN 26
2 MATERIAL METHODS 28
2.1 MATERIAL 28
2.1.1 FLY STRAINS 28
2.1.2 ANTIBODIES 30
2.1.2.1 PRIMARY 30
2.1.2.2 SECONDARY 31
2.1.3 LADDERS AND ENZYMES 31
2.1.4 KITS 31
2.2 METHODS 32
2.2.1 WORKING WITH FLIES 32
2.2.1.1 FLY KEEPING 32
2.2.1.2 PARAFFIN SECTIONS 32
2.2.1.3 FAST PHOTOTAXIS ASSAY 33
2.2.2 WORKING WITH PROTEINS 34
2.2.2.1 WESTERN BLOT 34
2.2.2.2 VISUALIZING F-ACTIN AND G-ACTIN LEVELS 35
2.2.3 IMMUNOPRECIPITATION AND SUBCELLULAR FRACTIONATION 36
2.2.4 WORKING WITH PRIMARY CELLS 36
2.2.4.1 MEASURING NEURITE LENGTH AND MOVEMENT OF MITOCHONDRIA 37
3 RESULTS 39
III
HTTP://D-NB.INFO/1033390631
IMAGE 2
3.1 INTERACTIONS WITH RHO AND ROK 40
3.1.1 LOE SHOWS MORE ISOPRENYLATION OF RHO1 40
3.1.2 MORE RHO IS LOCATED AT THE MEMBRANE IN LOE 41
3.1.3 THE INFLUENCE OF ROK1 ON THE NEURODEGENERATIVE PHENOTYPE IN LOE 44
3.2 EFFECT OF THE PROTEINS LIMK AND SLINGSHOT ON LOE 45
3.3 THE PROTEIN COFILIN 49
3.3.1 THE INFLUENCE OF COFILIN ON THE NEURODEGENERATIVE PHENOTYPE IN LOE
49 3.3.2 UP-REGULATION OF INACTIVE COFILIN IN LOE 51
3.4 THE ROLE OF ACTIN 53
3.5 LOE NEURONS SHOW A CHANGE IN NEURITE OUTGROWTH AND ORGANELLE
TRANSPORT 55 3.5.1 OUTGROWTH OF NEURITES IS AFFECTED IN LOE 55
3.5.2 SLOWER TRANSPORT THROUGH NEURITES IN LOE 58
3.6 BEHAVIORAL PHENOTYPE IN LOE 60
3.7 AMPK YRNAI SHOWS A DEGENERATIVE PHENOTYPE WITH VARIOUS DRIVERS 66
3.7.1 PHENOTYPE IN UAS-AMPK Y RNAI DRIVEN WITH GMR II - GAL4 AND GMR III
GAL4 AND EY-GAL4 66
3.7.2 PHENOTYPE IN UAS AMPK Y RNAI DRIVEN NEURONAL DRIVERS 68 3.8
CHOLESTEROL INFLUENCES NEURODEGENERATION 69
4 DISCUSSION 72
4.1 LOE INTERFERES WITH THE RHO PATHWAY 73
4.2 LOE HAS MORE INACTIVE COFILIN 78
4.3 CHOLESTEROL EFFECTS THE NEURODEGENERATION PHENOTYPE 79
4.4 NEURITE OUTGROWTH AND TRANSPORT IS AFFECTED IN LOE 81
5 REFERENCES 85
6 ABBREVIATIONS 91
ACKNOWLEDGEMENTS 93
LIST OF PUBLICATIONS 94
CURRICULUM VITAE - LEBENSLAUF 96
IV |
| any_adam_object | 1 |
| author | Cook, Mandy 21. Jh |
| author_GND | (DE-588)1028531184 |
| author_facet | Cook, Mandy 21. Jh |
| author_role | aut |
| author_sort | Cook, Mandy 21. Jh |
| author_variant | m c mc |
| building | Verbundindex |
| bvnumber | BV040606133 |
| collection | ebook |
| ctrlnum | (OCoLC)823232371 (DE-599)DNB1024851869 |
| discipline | Biologie |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV040606133 |
| illustrated | Illustrated |
| indexdate | 2025-02-14T17:56:55Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025433799 |
| oclc_num | 823232371 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
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| physical | VI, 93 S. Illustrationen, Graphen |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spellingShingle | Cook, Mandy 21. Jh The neurodegenerative Drosophila melanogaster AMPK mutant loechrig Molekulargenetik (DE-588)4039987-4 gnd AMP (DE-588)4142269-7 gnd Taufliege (DE-588)4078168-9 gnd Proteinkinasen (DE-588)4175996-5 gnd Nervendegeneration (DE-588)4300182-8 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4142269-7 (DE-588)4078168-9 (DE-588)4175996-5 (DE-588)4300182-8 (DE-588)4113937-9 |
| title | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig |
| title_alt | The neurodegenerative Drosophila melanogaster AMPK Mutante loechrig |
| title_auth | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig |
| title_exact_search | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig |
| title_full | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig von Mandy Cook. Betreuer: Doris Kretzschmar |
| title_fullStr | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig von Mandy Cook. Betreuer: Doris Kretzschmar |
| title_full_unstemmed | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig von Mandy Cook. Betreuer: Doris Kretzschmar |
| title_short | The neurodegenerative Drosophila melanogaster AMPK mutant loechrig |
| title_sort | the neurodegenerative drosophila melanogaster ampk mutant loechrig |
| topic | Molekulargenetik (DE-588)4039987-4 gnd AMP (DE-588)4142269-7 gnd Taufliege (DE-588)4078168-9 gnd Proteinkinasen (DE-588)4175996-5 gnd Nervendegeneration (DE-588)4300182-8 gnd |
| topic_facet | Molekulargenetik AMP Taufliege Proteinkinasen Nervendegeneration Hochschulschrift |
| url | http://d-nb.info/1024851869/34 https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-72027 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025433799&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT cookmandy theneurodegenerativedrosophilamelanogasterampkmutantloechrig AT cookmandy theneurodegenerativedrosophilamelanogasterampkmutanteloechrig |