Mouse genetic analyses of Spir functions
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2012
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| 100 | 1 | |a Pleiser, Sandra |e Verfasser |0 (DE-588)1027878881 |4 aut | |
| 245 | 1 | 0 | |a Mouse genetic analyses of Spir functions |c von Sandra Pleiser |
| 246 | 1 | 3 | |a Maus-genetische Analysen zur Funktion von Spir |
| 264 | 1 | |c 2012 | |
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Datensatz im Suchindex
| DE-BY-TUM_katkey | 1893156 |
|---|---|
| DE-BY-UBG_katkey | 2917091 |
| _version_ | 1816713820608921600 |
| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
SUMMARY I
ZUSAMMENFASSUNG ILL
1 INTRODUCTION 1
1.1 THE ACTIN CYTOSKELETON 1
1.2 ACTIN NUCLEATION 3
1.2.1 FORMINS 4
1.2.2 THE WH2 DOMAIN CONTAINING ACTIN NUCLEATION FACTOR SPIR 5
1.2.3 SPIR/FORMIN COOPERATION IN ACTIN NUCLEATION 7
1.3 SPIR FUNCTION IN VESICLE TRANSPORT PROCESSES 10
1.4 AIM OF THE WORK 11
2 RESULTS 12
2.1 TISSUE SPECIFIC EXPRESSION OF THE MOUSE SPIR-1 AND SPIR-2 GENES 12
2.1.1 RELATIVE MRNA EXPRESSION OF SPIR-1 AND SPIR-2 12
2.1.2 SPIR-2 GENE EXPRESSION DURING MOUSE EMBRYOGENESIS 14
2.1.3 SPATIAL SPIR-2 GENE EXPRESSION IN ADULT MOUSE TISSUES 17
2.2 MOUSE GENETIC ANALYSES 19
2.2.1 GENERATION OF SPIR-1 DEFICIENT MICE 19
2.2.2 INITIAL PHENOTYPING OF SPIR-1 DEFICIENT MICE 23
2.2.3 SPIR-1 FUNCTION IN NEURONAL DIFFERENTIATION 24
2.2.3.1 REDUCED NUMBER OF DENDRITIC BRANCHPOINTS IN SPIR-1 DEFICIENT
CULTURED PRIMARY CORTICAL NEURONS 24
2.2.3.2 IMPAIRED DENDRITIC SPINE FORMATION IN SPIR-1 DEFICIENT NEURONS
26
3 DISCUSSION 28
3.1 EXPRESSION PATTERNS OF MAMMALIAN SPIR GENES 28
3.1.1 SPIR-2 SHOWS A BROADER EXPRESSION PATTERN THAN SPIR-1 28
3.1.2 SPIR EXPRESSION IN THE MOUSE OOCYTE 31
3.1.3 SPIR EXPRESSION IN THE NERVOUS SYSTEM 32
3.1.4 SPIR-2 EXPRESSION IN THE INTESTINE AND TESTIS 33
3.1.5 SUMMARY 34
3.2 MOUSE GENETIC ANALYSES OF SPIR-1 FUNCTION 35
3.2.1 FUNCTION OF SPIR-1 IN NEURONAL DIFFERENTIATION 36
3.2.2 FUNCTION OF SPIR-1 IN DENDRITIC SPINE FORMATION 38
V
HTTP://D-NB.INFO/1033388734
IMAGE 2
TABLE OF CONTENTS
4 MATERIALS AND METHODS 40
4.1 MATERIALS 40
4.1.1 DISPOSABLE MATERIALS 40
4.1.2 CHEMICALS AND REAGENTS 42
4.1.3 CELL CULTURE MEDIA, REAGENTS AND SUPPLEMENTS 44
4.1.4 ANTIBODIES 44
4.1.5 ENZYMES 45
4.1.6 KITS 45
4.1.7 MOLECULAR WEIGHT MARKERS 46
4.1.8 OLIGONUCLEOTIDES 46
4.1.9 MOUSE LINES 47
4.1.10 SOLUTIONS AND BUFFERS 48
4.1.10.1 GENERAL BUFFERS 48
4.1.10.2 DNA BUFFERS 50
4.1.10.3 IN SITU HYBRIDIZATION BUFFERS 50
4.1.10.4 PROTEIN BUFFERS 52
4.2 METHODS 54
4.2.1 CELL CULTURE METHODS 54
4.2.1.1 POLY-D-LYSINE COATING 54
4.2.1.2 ISOLATION OF PRIMARY MOUSE CORTICAL NEURONS 54
4.2.2 NUCLEIC ACID ANALYSES 55
4.2.2.1 DNA ISOLATION FROM MOUSE BIOPSIES 55
4.2.2.2 RNA ISOLATION FROM CELLS AND TISSUES 55
4.2.2.3 RNA CONCENTRATION MEASUREMENT 56
4.2.2.4 CDNA-SYNTHESIS 56
4.2.2.5 POLYMERASE CHAIN REACTION (PCR) 56
4.2.2.6 AGAROSE GEL ELECTROPHORESIS 57
4.2.2.7 REAL-TIME PCR 57
4.2.2.8 GENERATION OF DIG-LABELED RNA PROBES 58
4.2.2.9 IN SITU HYBRIDIZATION 58
4.2.3 PROTEIN ANALYSES 60
4.2.3.1 PREPARATION OF WHOLE PROTEIN LYSATES 60
4.2.3.2 SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) 60
4.2.3.3 WESTERN BLOT 61
4.2.3.4 IMMUNODETECTION 61
4.2.4 IMMUNOCYTOCHEMISTRY 62
4.2.5 HISTOLOGY 62
5 BIBLIOGRAPHY 64
6 APPENDIX 71
6.1 LIST OF ABBREVIATIONS 71
6.2 ACKNOWLEDGEMENTS 73
VI
IMAGE 3
TABLE OF CONTENTS
6.3 CURRICULUM VITAE 74
6.4 LIST OF PUBLICATIONS 75
VII
|
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| illustrated | Illustrated |
| indexdate | 2024-11-25T17:37:10Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025392842 |
| oclc_num | 820400867 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 |
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| physical | VII, 75 Bl. graph. Darst. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spellingShingle | Pleiser, Sandra Mouse genetic analyses of Spir functions Knockout Molekulargenetik (DE-588)4671699-3 gnd Actin-bindende Proteine (DE-588)4359634-4 gnd |
| subject_GND | (DE-588)4671699-3 (DE-588)4359634-4 (DE-588)4113937-9 |
| title | Mouse genetic analyses of Spir functions |
| title_alt | Maus-genetische Analysen zur Funktion von Spir |
| title_auth | Mouse genetic analyses of Spir functions |
| title_exact_search | Mouse genetic analyses of Spir functions |
| title_full | Mouse genetic analyses of Spir functions von Sandra Pleiser |
| title_fullStr | Mouse genetic analyses of Spir functions von Sandra Pleiser |
| title_full_unstemmed | Mouse genetic analyses of Spir functions von Sandra Pleiser |
| title_short | Mouse genetic analyses of Spir functions |
| title_sort | mouse genetic analyses of spir functions |
| topic | Knockout Molekulargenetik (DE-588)4671699-3 gnd Actin-bindende Proteine (DE-588)4359634-4 gnd |
| topic_facet | Knockout Molekulargenetik Actin-bindende Proteine Hochschulschrift |
| url | https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-73634 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025392842&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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