Elucidating virus uptake and fusion by single virus tracing
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Verl. Dr. Hut
2012
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Physikalische Chemie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a Elucidating virus uptake and fusion by single virus tracing |c Dorothee Carolin Schupp |
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| 264 | 1 | |a München |b Verl. Dr. Hut |c 2012 | |
| 300 | |a VII, 197 S. |b Ill., graph. Darst. |c 210 mm x 148 mm, 323 g | ||
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Datensatz im Suchindex
| DE-19_call_number | 1801/Diss. 4213 0001/8 12-9315 |
|---|---|
| DE-19_location | 0 85 |
| DE-BY-UBM_katkey | 4680209 |
| DE-BY-UBM_media_number | 41619445260010 41626502990014 |
| _version_ | 1823055096552882176 |
| adam_text | IMAGE 1
CONTENTS
1 INTRODUCTION 1
2 FLUORESCENCE MICROSCOPY 5
2.1 PRINCIPLES OF FLUORESCENCE 6
2.2 FLUORESCENT MICROSCOPE TECHNIQUES 8
2.2.1 WIDE-FIELD MICROSCOPY 9
2.2.2 CONFOCAL MICROSCOPY 10
2.3 FLUORESCENT PROTEINS 12
3 INTRODUCTION TO VIRUSES 17
3.1 HERPESVIRALES 18
3.1.1 HERPES SIMPLEX VIRUS 1 21
3.2 RETROVIRIDAE 24
3.2.1 FOAMY VIRUS 26
4 VIRUS ENTRY AND REPLICATION 31
4.1 ENDOCYTIC MODES OF ENTRY 31
4.1.1 THE ENDOSOMAL SYSTEM 33
4.2 VIRUS MEMBRANE FUSION 33
4.2.1 VIRUS TRAFFICKING 37
4.3 HERPES SIMPLEX VIRUS 1 39
4.3.1 ATTACHMENT AND ENTRY 39
4.3.2 ASSEMBLY AND EGRESS 49
4.4 FOAMY VIRUS 52
4.4.1 ATTACHMENT AND ENTRY 52
4.4.2 ASSEMBLY AND EGRESS 54
5 MATERIALS AND METHODS 57
V
HTTP://D-NB.INFO/1027155588
IMAGE 2
CONTENTS
5.1 SAMPLE PREPARATION 57
5.1.1 HERPES SIMPLEX VIRUS 1 PARTICLES (HSV-1) 57
5.1.2 FOAMY VIRUS PARTICLES (FV) 59
5.1.3 CELL CULTURE 60
5.1.4 LIVE-CELL IMAGING CONDITIONS 61
5.1.5 FIXATION AND PERMEABILIZATION 62
5.1.6 QUENCHING OF VIRUS PARTICLES A T LOW PH 64
5.2 EXPERIMENTAL SETUP IN DETAILS 64
5.2.1 WIDE-FIELD MICROSCOPY 66
5.2.2 SPINNING-DISK CONFOCAL MICROSCOPY 68
5.3 DATA ANALYSIS 70
5.3.1 IMAGE CALIBRATION 71
5.3.2 COLOCALIZATION ANALYSIS OF VIRUS-ONLY SAMPLES IN 2D 71
5.3.3 COLOCALIZATION ANALYSIS OF VIRUS-INCUBATED CELLS IN 3D 72
5.3.4 SINGLE PARTICLE TRACKING 74
5.3.5 DYNAMIC COLOCALIZATION ANALYSIS OF SINGLE VIRUS TRAJECTORIES . . .
. 75
6 STUDY O F HERPES SIMPLEX VIRUS ENTRY 79
6.1 OPTIMIZATION OF VIRUS PREPARATION 80
6.1.1 FIRST SCREENING ROUND OF VIRUS OPTIMIZATION 82
6.1.2 SECOND SCREENING ROUND OF VIRUS OPTIMIZATION 90
6.1.3 COLOCALIZATION ANALYSIS OF PURIFIED DUAL-COLOR VIRUS PREPARATIONS
. 94
6.2 COLOCALIZATION ANALYSIS IN LIVE CELLS 97
6.3 PH-DEPENDENCY OF FLUORESCENT PROTEINS IN THE VIRAL CONTEXT 100
6.4 COLOCALIZATION ANALYSIS IN FIXED CELLS 102
6.5 STUDYING INFLUENCE OF PH DEPENDENCY IN LIVE CELLS 106
6.6 ANALYSIS OF INDIVIDUAL HSV-1 TRAJECTORIES 114
7 STUDY O F FOAMY VIRUS ENTRY 121
7.1 CHARACTERIZATION OF VIRAL PARTICLES 122
7.1.1 COLOCALIZATION ANALYSIS OF INITIAL DUAL-COLOR VIRUS PREPARATIONS .
. 123
7.1.2 PH-DEPENDENCY OF FLUORESCENTLY TAGGED VIRUS PARTICLES 126
7.2 TIME-LAPSED ANALYSIS OF COLOCALIZATION IN LIVE CELLS 128
7.3 ANALYSIS OF INDIVIDUAL FUSION EVENTS 130
7.3.1 PFV ENV FUSION AT THE PLASMA MEMBRANE AND WITH ENDOSOMES . . 133
7.3.2 SFV ENV FUSION IN ENDOSOMES 137
7.3.3 KINETICS OF THE ENTRY PROCESS 137
VI
IMAGE 3
CONTENTS
7.3.4 DYNAMICS OF FUSION 140
8 COMPARISON O F HSV-1 AND FV ENTRY 147
8.1 COLOCALIZATION PERCENTAGE OF DUAL-COLOR VIRUS PREPARATIONS 147
8.2 COLOCALIZATION ANALYSIS IN CELLS 148
8.3 ANALYSIS OF INDIVIDUAL VIRUS TRAJECTORIES 150
BIBLIOGRAPHY 155
LIST OF ABBREVIATIONS 191
ACKNOWLEDGEMENTS 199
VII
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| any_adam_object | 1 |
| author | Schupp, Dorothee Carolin |
| author_facet | Schupp, Dorothee Carolin |
| author_role | aut |
| author_sort | Schupp, Dorothee Carolin |
| author_variant | d c s dc dcs |
| building | Verbundindex |
| bvnumber | BV040524558 |
| ctrlnum | (OCoLC)822260273 (DE-599)DNB1027155588 |
| dewey-full | 571.9920287 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.9920287 |
| dewey-search | 571.9920287 |
| dewey-sort | 3571.9920287 |
| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Biologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV040524558 |
| illustrated | Illustrated |
| indexdate | 2025-02-03T17:28:01Z |
| institution | BVB |
| isbn | 9783843906333 3843906335 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025370773 |
| oclc_num | 822260273 |
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| owner_facet | DE-19 DE-BY-UBM DE-12 |
| physical | VII, 197 S. Ill., graph. Darst. 210 mm x 148 mm, 323 g |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Verl. Dr. Hut |
| record_format | marc |
| series2 | Physikalische Chemie |
| spellingShingle | Schupp, Dorothee Carolin Elucidating virus uptake and fusion by single virus tracing Spumaviren (DE-588)4297410-0 gnd Herpesviren (DE-588)4159627-4 gnd Single Virus Tracing (DE-588)1028854617 gnd |
| subject_GND | (DE-588)4297410-0 (DE-588)4159627-4 (DE-588)1028854617 (DE-588)4113937-9 |
| title | Elucidating virus uptake and fusion by single virus tracing |
| title_auth | Elucidating virus uptake and fusion by single virus tracing |
| title_exact_search | Elucidating virus uptake and fusion by single virus tracing |
| title_full | Elucidating virus uptake and fusion by single virus tracing Dorothee Carolin Schupp |
| title_fullStr | Elucidating virus uptake and fusion by single virus tracing Dorothee Carolin Schupp |
| title_full_unstemmed | Elucidating virus uptake and fusion by single virus tracing Dorothee Carolin Schupp |
| title_short | Elucidating virus uptake and fusion by single virus tracing |
| title_sort | elucidating virus uptake and fusion by single virus tracing |
| topic | Spumaviren (DE-588)4297410-0 gnd Herpesviren (DE-588)4159627-4 gnd Single Virus Tracing (DE-588)1028854617 gnd |
| topic_facet | Spumaviren Herpesviren Single Virus Tracing Hochschulschrift |
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