Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae
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| Sprache: | English |
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2012
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| 100 | 1 | |a Beck, Johannes Gerhard |e Verfasser |0 (DE-588)1026863740 |4 aut | |
| 245 | 1 | 0 | |a Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae |c Johannes Gerhard Beck |
| 264 | 1 | |c 2012 | |
| 300 | |a XVI, 157 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Techn. Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a NMR-Spektroskopie |0 (DE-588)4075421-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Hitzeschock-Proteine |0 (DE-588)4255487-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Cyclopeptide |0 (DE-588)4148612-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Analoga |0 (DE-588)4400580-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Hitzeschock-Proteine |0 (DE-588)4255487-1 |D s |
| 689 | 0 | 1 | |a Cyclopeptide |0 (DE-588)4148612-2 |D s |
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 0001 DM 30161 |
|---|---|
| DE-BY-TUM_katkey | 1866245 |
| DE-BY-TUM_location | Mag |
| DE-BY-TUM_media_number | TEMP3214026 |
| DE-BY-UBR_katkey | 4993488 |
| _version_ | 1822704970813669376 |
| adam_text | IMAGE 1
CONTENTS
1 INTRODUCTION 1
1.1 STRUCTURE AND DYNAMICS OF PEPTIDES 5
1.1.1 CYCLOPEPTIDE CONFORMATIONS BY NMR SPECTROSCOPY 6
1.1.2 RESONANCE ASSIGNMENT 6
1.1.3 EXTRACTION OF PRECISE DISTANCES FROM ROESY SPECTRA 10
1.1.4 EXTRACTION OF DIHEDRAL ANGLES FROM 3 J COUPLINGS 14
1.1.5 RELATIVE ORIENTATIONS FROM RESIDUAL DIPOLAR COUPLINGS 19
1.1.6 DISTANCE GEOMETRY AND MOLECULAR DYNAMICS CALCULATIONS 20 1.1.7
CONFORMATIONAL AVERAGING OF ROES AND J COUPLINGS 20
1.1.8 IMPORTANCE OF SOLUTION CONFORMATIONS 22
2 HIGHLY /V-METHYLATED CYCLIC PEPTIDES TARGETING DISTINCT MELANOCORTIN
AND SOMATOSTATIN RECEPTOR SUBTYPES 25
2.1 G PROTEIN-COUPLED RECEPTORS 27
2.2 MELANOCORTINS, THEIR RECEPTORS, AND MELANOCORTIN ANALOGS 29
2.2.1 UNSELECTIVE MT-II AND SELECTIVE 4-A/ME-(HIS 6 ,ARG 8 ,TRP 9 ,LYS
10 )-MT-LL 32 2.2.2 STRUCTURE OF 4-A/ME-(HIS 6 ,ARG 8 ,TRP 9 ,L_YS 10
)-MT-LL 33
2.2.3 DYNAMICS OF 4-/VME-(HIS 6 ,ARG 8 ,TRP 9 ,LYS 10 )-MT-LL 38
2.3 SOMATOSTATIN, ITS RECEPTORS, AND SOMATOSTATIN ANALOGS 42
2.3.1 SOMATOSTATIN AND NEUROGENIC INFLAMMATION 43
2.3.2 STRUCTURE OF 3-A/ME-(D-TRP 8 ,LYS 9 ,PHE 11 )-SEGLITIDE 44
2.3.3 DYNAMICS OF 3-A/ME-(D-TRP 8 ,LYS 9 ,PHE 11 )-SEGLITIDE 47
3 DI-A/-METHYLATED ANALOGS OF C(RGDFV) TARGETING INTEGRIN SUBTYPE AV|33
SELECTIVELY 51
3.1 CILENGITIDE AND INTEGRINS 53
3.2 ACTIVITY OF DI-A/-METHYLATED C(RGDFV) ANALOGS 54
3.3 STRUCTURES OF SELECTED DI-A/-METHYLATED C(RGDFV) ANALOGS 56
4 ORALLY BIOAVAILABLE PEPTIDES 63
4.1 INTRODUCTION 65
4.2 LIPOPHILICITY AND PERMEATION PATHWAY 68
4.3 NMR CONFORMATIONAL STUDIES 69
4.4 FIRST HIGHLY CACO-2 PERMEABLE TEMPLATE STRUCTURE 69
4.5 SECOND HIGHLY CACO-2 PERMEABLE TEMPLATE STRUCTURE 72
4.6 STRIKING SIMILARITY WITH ORALLY BIOAVAILABLE PEPTIDES 78
4.7 CONCLUSION 81
5 HSP26 FROM S. CEREVISIAE 83
5.1 INTRODUCTION 84
5.2 DIMERIC HSP26 DELETION PROTEIN H S P 2 6 3 0 - 1 9 5 8 5
5.2.1 EXPRESSION AND PURIFICATION 85
5.2.2 NMR SPECTROSCOPY OF HSP26 30-195 87
5.3 MONOMERIC HSP26 DELETION PROTEIN HSP26 30 -195(A137-153) 89
5.3.1 DESIGN OF HSP2630-195(A137-153) 89
5.3.2 DELETION OF THE BASES ENCODING RESIDUES 137-153 BY PCR 90
5.3.3 EXPRESSION AND PURIFICATION 90
5.3.4 NMR SPECTROSCOPY 93
XI
HTTP://D-NB.INFO/1026863902
IMAGE 2
6 SUMMARY
97
7 EXPERIMENTAL SECTION 99
7.1 MATERIALS AND METHODS - PEPTIDE PROJECTS 99
7.1.1 NMR SPECTROSCOPY 99
7.1.2 DISTANCE RESTRAINTS 103
7.1.3 DISTANCE GEOMETRY CALCULATIONS 103
7.1.4 RESTRAINED MOLECULAR DYNAMICS SIMULATIONS 104
7.1.5 UNRESTRAINED MOLECULAR DYNAMICS (MD) SIMULATIONS 104
7.2 MATERIALS AND METHODS - HSP26 PROJECT 108
7.2.1 CHEMICALS AND ENZYMES 108
7.2.2 BUFFER SOLUTIONS AND CULTURE MEDIA 108
7.2.3 VECTORS AND DNA OLIGONUCLEOTIDES 110
7.2.4 POLYMERASE CHAIN REACTION 110
7.2.5 AGAROSE GEL ELECTROPHORESIS 111
7.2.6 INTRA-MOLECULAR BLUNT END LIGATION 112
7.2.7 E. COLI STRAINS 112
7.2.8 PREPARATION OF CHEMICALLY COMPETENT CELLS 112
7.2.9 CHEMICAL TRANSFORMATION OF E. COLI 112
7.2.10 STORAGE OF E. COLI 113
7.2.11 EXPRESSION ANALYSIS 113
7.2.12 SDS-PAGE 113
7.2.13 FAST PROTEIN LIQUID CHROMATOGRAPHY 114
7.2.14 EXPRESSION AND PURIFICATION OF HSP26 30-195 114
7.2.15 EXPRESSION AND PURIFICATION OF HSP26 30 -I95(&I37-I53) 115
7.2.16 CIRCULAR DICHROISM SPECTROSCOPY 115
7.2.17 UVA/IS SPECTROSCOPY 116
7.2.18 NMR SPECTROSCOPY 116
APPENDIX A 119
APPENDIX B 121
APPENDIX C 123
APPENDIX D 127
APPENDIX E 143
BIBLIOGRAPHY 147
XII
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| any_adam_object | 1 |
| author | Beck, Johannes Gerhard |
| author_GND | (DE-588)1026863740 |
| author_facet | Beck, Johannes Gerhard |
| author_role | aut |
| author_sort | Beck, Johannes Gerhard |
| author_variant | j g b jg jgb |
| building | Verbundindex |
| bvnumber | BV040308544 |
| classification_tum | CHE 820d CHE 808d CHE 806d CHE 834d CHE 244d |
| collection | ebook |
| ctrlnum | (OCoLC)802638690 (DE-599)BVBBV040308544 |
| dewey-full | 547.7046 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 547 - Organic chemistry |
| dewey-raw | 547.7046 |
| dewey-search | 547.7046 |
| dewey-sort | 3547.7046 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie |
| format | Thesis Book |
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| institution | BVB |
| language | English |
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| physical | XVI, 157 S. graph. Darst. |
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| spellingShingle | Beck, Johannes Gerhard Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae NMR-Spektroskopie (DE-588)4075421-2 gnd Hitzeschock-Proteine (DE-588)4255487-1 gnd Cyclopeptide (DE-588)4148612-2 gnd Analoga (DE-588)4400580-5 gnd |
| subject_GND | (DE-588)4075421-2 (DE-588)4255487-1 (DE-588)4148612-2 (DE-588)4400580-5 (DE-588)4113937-9 |
| title | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae |
| title_auth | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae |
| title_exact_search | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae |
| title_full | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae Johannes Gerhard Beck |
| title_fullStr | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae Johannes Gerhard Beck |
| title_full_unstemmed | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae Johannes Gerhard Beck |
| title_short | Structural studies of N-methylated cyclopeptides and of the small heat shock protein Hsp26 from S. cerevisiae |
| title_sort | structural studies of n methylated cyclopeptides and of the small heat shock protein hsp26 from s cerevisiae |
| topic | NMR-Spektroskopie (DE-588)4075421-2 gnd Hitzeschock-Proteine (DE-588)4255487-1 gnd Cyclopeptide (DE-588)4148612-2 gnd Analoga (DE-588)4400580-5 gnd |
| topic_facet | NMR-Spektroskopie Hitzeschock-Proteine Cyclopeptide Analoga Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1097376 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120502-1097376-1-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025163400&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT beckjohannesgerhard structuralstudiesofnmethylatedcyclopeptidesandofthesmallheatshockproteinhsp26fromscerevisiae |