Retinal development methods and protocols
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Format: | Buch |
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Sprache: | English |
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New York, NY
Humana Press
2012
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Schriftenreihe: | Methods in molecular biology
884 |
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Online-Zugang: | Inhaltsverzeichnis |
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---|---|---|---|
001 | BV039992524 | ||
003 | DE-604 | ||
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007 | t| | ||
008 | 120329s2012 xx ad|| |||| 00||| eng d | ||
020 | |a 9781617798474 |c hbk |9 978-1-61779-847-4 | ||
035 | |a (OCoLC)785862080 | ||
035 | |a (DE-599)BVBBV039992524 | ||
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245 | 1 | 0 | |a Retinal development |b methods and protocols |c ed. by Shu-Zhen Wang |
264 | 1 | |a New York, NY |b Humana Press |c 2012 | |
300 | |a XIV, 367 S. |b Ill., graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
490 | 1 | |a Methods in molecular biology |v 884 | |
490 | 0 | |a Springer protocols | |
650 | 0 | 7 | |a Molekularbiologische Methode |0 (DE-588)7650063-9 |2 gnd |9 rswk-swf |
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700 | 1 | |a Wang, Shu-Zhen |e Sonstige |4 oth | |
830 | 0 | |a Methods in molecular biology |v 884 |w (DE-604)BV035362695 |9 884 | |
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Datensatz im Suchindex
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DE-BY-UBR_katkey | 4955060 |
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adam_text | Contents
Preface
.......................................................... v
Contributors.
..................................................... xi
Part I Manipulation of Gene Expression In Vivo
1
Conditional Control of Gene Expression in the Mouse Retina
............. 3
Qian Ditig and Lin Gan
2
Generation of Transgenic Xenopus Using Restriction Enzyme-Mediated
Integration
................................................... 17
Mohammad Haeri and Barry E. Knox
3
In Vivo Functional Analysis of Transcription Factor: Response
Element Interaction Using Transgenic Xenopus laevis
................... 41
Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly
4
Subretinal Delivery and Electroporation in
Pigmented
and Nonpigmented
Adult Mouse Eyes
.............................................. 53
John M. Nickerson, Penny Goodman,
Micah
A. Chrenek,
Christiana J.
Bernal,
Lennart Berglin, T.
Michael
Redmond,
and Jeffrey H.
Boatrißht
5
In
Ovo
Eye
Electroporation
...................................... 71
Teri
L.
Belecky-Adams, Scott
R.
Hudson, and
Sárika
Tiwari
6
Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene
Expression in Xenopus Embryos
................................... 91
Sally A. Moody
Part II Tracing Cell Fate
7
Testing Retina Fate Commitment in Xenopus by Blastomere Deletion,
Transplantation, and
Expiant
Culture
............................... 115
Sally A. Moody
8
Application of Cre-loxP Recombination for Lineage Tracing
of Adult Zebrafish Retinal Stem Cells
............................... 129
Rajesh Ramachandran, Aaron
Reifler,
Jin Wan,
and Daniel Goldman
9
Fate Tracing of
neurojeninž-Expressing
Cells in the Mouse Retina
Using CreER™: LacZ
........................................... 141
Wenxin Ma and Shu-Zhen Wang
VII
viii Contents
Part III In Vitro Systems
10
In Vitro
Expiant
Culture and Related Protocols for the Study
of Mouse Retinal Development
.................................... 155
Kangxinjin and Mengqing Xiang
11
In Vitro Biochemical Assays to Monitor Rhodopsin Function
............. 167
Joshua Sammons andAlecia K. Gross
12
Transfection of Primary Embryonic Chicken Retinal Cells
Using Cationic
Lipid
............................................ 183
Ті-
Wen Hsieh and Xian-Jie Yang
13
Production of High-Titer RCAS
Retrovirus
.......................... 193
Run-Tao Tan and Shu-Zhen Wang
14
Chick Retinal Pigment Epithelium
Transdifferentiation
Assay
for Proneural Activities
.......................................... 201
Shu-Zhen Wang and Run-Tao Tan
Part IV Regeneration/Stem Cells
15
Studying the Generation of Regenerated Retinal Neuron from
Müller
Glia
in the Mouse Eye
........................................... 213
Mike O. Karl and Thomas A. Reh
16
Production and Transplantation of Retinal Cells from Human
and Mouse Embryonic Stem Cells
.................................. 229
Anna La Torre, Deepak
A. Lamba,
Anujayabalu,
and Thomas A. Reh
17
Light-Induced Photoreceptor Degeneration in the Retina
of the Zebrafish
................................................ 247
Scott Taylor, Jing Chen, Jing Luo, and Peter Hitchcock
18
Microarray-Based Gene Profiling Analysis of
Müller Glia-Derived
Retinal Stem Cells in Light-Damaged Retinas from Adult Zebrafish
........ 255
Zhao Qin and Pamela A. Raymond
Part V Function/Imaging
19
Measuring Rodent Electroretinograms to Assess Retinal Function
.......... 265
Molly E. Clark and
Timothy
W. Krafi
20
Functional Imaging of Retinal Photoreceptors and Inner Neurons
Using Stimulus-Evoked Intrinsic Optical Signals
....................... 277
Xin-Cheng Yao and Ti-Cbao Li
Contents ix
Part VI Emerging Methodologies
21
Use of Laser Capture Microdissection for Analysis of Retinal
mRNA/miRNA Expression and
DNA Methylation..................... 289
Laszlo Hacklerjr, Tomohiro Masuda, Verity F. Oliver,
Shannath L. Merbs, and Donald J.
Zack
22
Revealing Looping Organization of Mammalian Photoreceptor
Genes Using Chromosome Conformation Capture (3C) Assays
........... 305
Guang-Hua Peng and Shiming Chen
23
Retinal
Transcriptome
Profiling by Directional Next-Generation
Sequencing Using
100
ng of Total
RNA
............................. 319
Matthew
J.
Broofa, Harsha Karur Rajasimha,
and Anand Swaroop
24
Exorne
Sequencing: Capture and Sequencing of All Human Coding
Regions for Disease Gene Discovery
................................ 335
Rinki
Ratna
Priya, Harsha Karur Rajasimha,
Matthew J. Brooks, and Anand Swaroop
25
Determination of Posttranslational Modifications of Photoreceptor
Differentiation Factor NRL: Focus on SUMOylation
................... 353
Jerome E. Roger, Jacob Nellissery, and Anand Swaroop
Index
........................................................... 363
|
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id | DE-604.BV039992524 |
illustrated | Illustrated |
indexdate | 2024-12-24T02:37:03Z |
institution | BVB |
isbn | 9781617798474 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024849621 |
oclc_num | 785862080 |
open_access_boolean | |
owner | DE-355 DE-BY-UBR DE-12 |
owner_facet | DE-355 DE-BY-UBR DE-12 |
physical | XIV, 367 S. Ill., graph. Darst. |
publishDate | 2012 |
publishDateSearch | 2012 |
publishDateSort | 2012 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spellingShingle | Retinal development methods and protocols Methods in molecular biology Molekularbiologische Methode (DE-588)7650063-9 gnd Entwicklung (DE-588)4113450-3 gnd Netzhaut (DE-588)4171512-3 gnd |
subject_GND | (DE-588)7650063-9 (DE-588)4113450-3 (DE-588)4171512-3 |
title | Retinal development methods and protocols |
title_auth | Retinal development methods and protocols |
title_exact_search | Retinal development methods and protocols |
title_full | Retinal development methods and protocols ed. by Shu-Zhen Wang |
title_fullStr | Retinal development methods and protocols ed. by Shu-Zhen Wang |
title_full_unstemmed | Retinal development methods and protocols ed. by Shu-Zhen Wang |
title_short | Retinal development |
title_sort | retinal development methods and protocols |
title_sub | methods and protocols |
topic | Molekularbiologische Methode (DE-588)7650063-9 gnd Entwicklung (DE-588)4113450-3 gnd Netzhaut (DE-588)4171512-3 gnd |
topic_facet | Molekularbiologische Methode Entwicklung Netzhaut |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024849621&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT wangshuzhen retinaldevelopmentmethodsandprotocols |