Methods in methane metabolism A Methanogenesis

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Sprache:English
Veröffentlicht: Amsterdam [u.a.] Elsevier, AP 2011
Ausgabe:1. ed.
Schriftenreihe:Methods in enzymology 494
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Datensatz im Suchindex

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adam_text Contents Contributors xi Preface xv Volumes in Series xvii ι. Techniques for Cultivating Methanogens 1 Ralph S. Wolfe 1. introduction 1 2. Methanogens on a Budget 2 3. Use of an Anoxic Chamber 18 Acknowledgment 21 References 21 2. Genetic Methods for Methanosarc ina Species 23 Nicole Buan, Gargi Kulkami, and William Metcalf 1. Introduction 24 2. Liposome-Mediated Transformation 28 3. Deleting Genes 29 4. Test for Gene Essentiality 34 5. Adding Genes 38 Acknowledgments 41 References 41 3. Genetic Systems for Hydrogenotrophic Methanogens 43 Felipe Sarmiento В., John A. Leigh, and William B. Whitman 1. Introduction 44 2. Genome Sequences 46 3. Growth and Storage of Methanogen Cultures 46 4. Genetic Tools 54 5. Transformation Methods 60 6. Other Genetic Techniques 66 Acknowledgments 71 References 71 vi Contents 4. Molecular Tools for Investigating ΑΝΛΛΕ Community Structure and Function 75 Steven J. Hallam, Antoine P. Page, Lea Constan, Young С Song, Angela D. Norbeck, Heather Brewer, and Ljiljana Pasa-Tolic 1. Introduction 76 2. Exploring ANME Population Structure 78 3. Detecting ANME Proteins in Marine Sediments 83 Acknowledgments 88 References 88 5. Studying Gene Regulation in Methanogenic Archaea 91 Michael Rother, Christian Sattler, and Tilmann Stock 1. Introduction 92 2. Quantitative Reverse Transcription PCR for УИ. maripaludis 94 3. Analyzing Gene Expression Using u idA Reporter Gene Fusions in M. acetivorans 97 4. Creating Random Disruption Mutants of M. maripaludis by In Vitro Transposon Mutagenesis 101 Acknowledgments 105 References 105 6. Growth of Methanogens Under Defined Hydrogen Conditions 111 John A. Leigh 1. Introduction 112 2. Hydrogen Limitation in Batch Culture 113 3. Hydrogen Limitation in Continuous Culture 116 Acknowledgments 118 References 118 7. Preparation of [Fe]-Hydrogenase from Methanogenic Archaea 119 Seigo Shirna, Michael Schick, and Haruka Tamura 1. Introduction 120 2. Oxygen, Light, and Copper Sensitivity of [FeJ-Hydrogenase and FeGP Cofactor 124 3. Preparation of [FeJ-Hydrogenase 125 4. Preparation of FeGP Cofactor 128 5. Preparation of Reconstituted [FeJ-Hydrogenase 131 6. Assay of the Enzyme Activity 134 Acknowledgments 135 References 136 Contents vii 8. Assay of Methylotrophic Methyltransferases from Methanogenic Archaea 139 Donald J. Ferguson, David G. Longstaff, and Joseph A. Krzycki 1. Introduction 140 2. Preparation of Anoxic Cell Extracts of M. barker! 141 3. Isolation and Resolution of MtmBC Complexes 143 4. Ellman s Assay of Methylotrophic Substrate.-CoM Methyl Transfer 145 5. Assay of MethylthiohCoM Methyltransferases 150 6. Assay of Methylotrophic Substrate:Corrinoid Methyltransferase Activity 152 7. Spectral Assay of MethylcobalamimCoM Methyltransferases 155 Acknowledgments 156 References 157 9. Methyl-Coenzyme M Reductase from Methanothermobacter tnarburgensis 159 Evert C. Duin, Divya Prakash, and Charlene Brungess 1. Introduction 160 2. Equipment 162 3. Cell Growth: Basic Procedure 164 4. Whole-Cell Experiments 167 5. Cell Growth: Induction of Different Forms of MCR 171 6. Enzyme Purification 172 7. Different MCR Forms 178 8. Materials 180 Acknowledgment 185 References 185 10. Methods for Analysis of Acetyl-CoA Synthase: Applications to Bacterial and Archaeal Systems 189 David A. Grahame 1. Introduction 190 2. Assays of A Cluster Protein (ACS) Catalytic Activities 195 3. Acetyltransferase Reactions 196 4. Acetyl-CoA Synthesis Reactions 205 5. The Acetyl-CoA CarbonykCO Exchange Reaction 211 6. Concluding Remarks 213 Acknowledgments 214 References 215 viii Contents и. Acetate Kinase and Phosphotransacetylase 219 James G. Ferry 1. Introduction 220 2. Acetate Kinase of Methanosarcina thermophila 221 3. Phosphotransacetylase of M. thermophila 226 Acknowledgments 229 References 229 іг. Sodium Ion Translocation and ATP Synthesis in Methanogens 233 Katharina Schlegel and Volker Müller 1. introduction 234 2. Analysis of the Na+ Dependence of Growth and Methanogenesis 237 3. Na+ Transport in Cell Suspensions 239 4. Determining Bioenergetic Parameters via Different Localization of Radioactively Labeled Substances: Intracellular Volume, ΔρΗ, and ΑΨ 242 5. ATP Synthesis 248 6. ATP Hydrolysis 252 Acknowledgments 253 References 253 13. Proton Translocation in Methanogens 257 Cornelia Weite and Uwe Deppenmeier 1. Introduction 258 2. Analysis of Membrane-Bound Electron Transport in Methanosarcina maze i 260 3. Measurement of Proton Translocation 271 4. Analysis of ATP Formation in Vesicle Preparation 276 Acknowledgments 277 References 277 14. Measuring Isotope Fractionation by Autotrophic Microorganisms and Enzymes 281 Kathleen M. Scott, Gordon Fox, and Peter R. Girguis 1. Introduction 282 2. Enzyme-Level Studies 283 3. Culture/Cell-Level Studies 290 4. Calculations 292 Acknowledgments 294 References 295 Contents ЇХ 15. 2-Oxoacid Metabolism in Methanogenic CoM and CoB Biosynthesis 301 David E. Graham 1. Introduction 302 2. Chemistry of 2-Oxoacids 302 3. Biosynthesis of Coenzyme M 304 4. Synthesis of 2-Sulfopyruvate 307 5. Discussion of CoM Biosynthesis 308 6. Coenzyme В Biosynthesis 309 7. Homocitrate Synthase 310 8. Homocitrate Synthase Enzymology 311 9. Substrate Specificity Determinants in HCS 312 10. Chemical Synthesis of 2-Oxoacids 313 11. Chemical Synthesis of Homocitrate and Its Analogs 314 12. Homoaconitase 315 13. Homoaconitase Enzymology 315 14. Chemical Synthesis of Homoaconitate and Its Analogs 316 15. Homoisocitrate Dehydrogenase 317 16. Enzymology of Homoisocitrate Dehydrogenase 317 17. Synthesis of Homoisocitrate 318 18. Chromatographie Analysis of 2-Oxoacids 318 19. Conclusions 321 Acknowledgments 321 References 322 16. Biomethanation and Its Potential 327 Irini Angelidaki, Dimitar Karakashev, Damien J. Batstone, Caroline M. Plugge, and Alfons J. M. Stams 1. Introduction 328 2. The AD Process: Microbiology and Metabolic Pathways 329 3. Biochemical Methane Potential 338 4. Biogas Applications 342 5. Biomethanation—Global Aspects 346 Acknowledgments 347 References 347 Author Index 353 Subject Index 367
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spellingShingle Methods in methane metabolism
Methods in enzymology
title Methods in methane metabolism
title_auth Methods in methane metabolism
title_exact_search Methods in methane metabolism
title_full Methods in methane metabolism A Methanogenesis ed. by Amy C. Rosenzweig ...
title_fullStr Methods in methane metabolism A Methanogenesis ed. by Amy C. Rosenzweig ...
title_full_unstemmed Methods in methane metabolism A Methanogenesis ed. by Amy C. Rosenzweig ...
title_short Methods in methane metabolism
title_sort methods in methane metabolism methanogenesis
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