Methods in methane metabolism A Methanogenesis
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Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, AP
2011
|
Ausgabe: | 1. ed. |
Schriftenreihe: | Methods in enzymology
494 |
Online-Zugang: | Inhaltsverzeichnis |
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MARC
LEADER | 00000nam a2200000 cc4500 | ||
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007 | t| | ||
008 | 110601s2011 xx ad|| |||| 00||| eng d | ||
020 | |a 9780123851123 |9 978-0-12-385112-3 | ||
035 | |a (OCoLC)729995479 | ||
035 | |a (DE-599)BVBBV037434174 | ||
040 | |a DE-604 |b ger |e rakwb | ||
041 | 0 | |a eng | |
049 | |a DE-19 |a DE-12 |a DE-355 |a DE-91G | ||
082 | 0 | |a 579.321 | |
245 | 1 | 0 | |a Methods in methane metabolism |n A |p Methanogenesis |c ed. by Amy C. Rosenzweig ... |
250 | |a 1. ed. | ||
264 | 1 | |a Amsterdam [u.a.] |b Elsevier, AP |c 2011 | |
300 | |a XLV, 373 S. |b Ill., graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
490 | 1 | |a Methods in enzymology |v 494 | |
490 | 0 | |a Methods in enzymology |v ... | |
700 | 1 | |a Rosenzweig, Amy C. |e Sonstige |4 oth | |
773 | 0 | 8 | |w (DE-604)BV037434154 |g 1 |
830 | 0 | |a Methods in enzymology |v 494 |w (DE-604)BV000000938 |9 494 | |
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943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-022586195 |
Datensatz im Suchindex
DE-BY-TUM_call_number | 0302 CHE 825f 03.1955 A 741 |
---|---|
DE-BY-TUM_katkey | 1772003 |
DE-BY-TUM_location | 03 |
DE-BY-TUM_media_number | 040030242036 |
_version_ | 1820897678081392640 |
adam_text | Contents
Contributors
xi
Preface
xv
Volumes in Series
xvii
ι.
Techniques for Cultivating
Methanogens
1
Ralph S. Wolfe
1.
introduction
1
2.
Methanogens
on a Budget
2
3.
Use of an Anoxic Chamber
18
Acknowledgment
21
References
21
2.
Genetic Methods for Methanosarc ina Species
23
Nicole Buan, Gargi
Kulkami,
and William Metcalf
1.
Introduction
24
2.
Liposome-Mediated Transformation
28
3.
Deleting Genes
29
4.
Test for Gene Essentiality
34
5.
Adding Genes
38
Acknowledgments
41
References
41
3.
Genetic Systems for Hydrogenotrophic
Methanogens
43
Felipe
Sarmiento
В.,
John A. Leigh, and William B. Whitman
1.
Introduction
44
2.
Genome Sequences
46
3.
Growth and Storage of
Methanogen
Cultures
46
4.
Genetic Tools
54
5.
Transformation Methods
60
6.
Other Genetic Techniques
66
Acknowledgments
71
References
71
vi
Contents
4.
Molecular
Tools for Investigating
ΑΝΛΛΕ
Community
Structure and Function
75
Steven J.
Hallam,
Antoine
P. Page,
Lea Constan,
Young
С
Song, Angela
D. Norbeck, Heather Brewer, and
Ljiljana Pasa-Tolic
1.
Introduction
76
2.
Exploring ANME Population Structure
78
3.
Detecting ANME Proteins in Marine Sediments
83
Acknowledgments
88
References
88
5.
Studying Gene Regulation in Methanogenic Archaea
91
Michael
Rother,
Christian
Sattler,
and Tilmann Stock
1.
Introduction
92
2.
Quantitative Reverse Transcription PCR for
УИ.
maripaludis
94
3.
Analyzing Gene Expression Using u idA Reporter
Gene Fusions in M. acetivorans
97
4.
Creating Random Disruption Mutants of M. maripaludis
by In Vitro Transposon Mutagenesis
101
Acknowledgments
105
References
105
6.
Growth of
Methanogens
Under Defined Hydrogen Conditions 111
John A. Leigh
1.
Introduction
112
2.
Hydrogen Limitation in Batch Culture
113
3.
Hydrogen Limitation in Continuous Culture
116
Acknowledgments
118
References
118
7.
Preparation of
[Fe]-Hydrogenase
from Methanogenic Archaea
119
Seigo Shirna, Michael
Schick,
and Haruka Tamura
1.
Introduction
120
2.
Oxygen, Light, and Copper Sensitivity of [FeJ-Hydrogenase
and FeGP Cofactor
124
3.
Preparation of [FeJ-Hydrogenase
125
4.
Preparation of FeGP Cofactor
128
5.
Preparation of Reconstituted [FeJ-Hydrogenase
131
6.
Assay of the Enzyme Activity
134
Acknowledgments
135
References
136
Contents
vii
8.
Assay of Methylotrophic Methyltransferases from
Methanogenic Archaea
139
Donald J. Ferguson, David G. Longstaff, and Joseph A. Krzycki
1.
Introduction
140
2.
Preparation of Anoxic Cell Extracts of M. barker!
141
3.
Isolation and Resolution of MtmBC Complexes
143
4.
Ellman s Assay of Methylotrophic Substrate.-CoM Methyl Transfer
145
5.
Assay of MethylthiohCoM Methyltransferases
150
6.
Assay of Methylotrophic Substrate:Corrinoid
Methyltransferase Activity
152
7.
Spectral Assay of MethylcobalamimCoM Methyltransferases
155
Acknowledgments
156
References
157
9.
Methyl-Coenzyme
M Reductase
from
Methanothermobacter tnarburgensis
159
Evert C.
Duin, Divya
Prakash, and Charlene Brungess
1.
Introduction
160
2.
Equipment
162
3.
Cell Growth: Basic Procedure
164
4.
Whole-Cell Experiments
167
5.
Cell Growth: Induction of Different Forms of MCR
171
6.
Enzyme Purification
172
7.
Different MCR Forms
178
8.
Materials
180
Acknowledgment
185
References
185
10.
Methods for Analysis of Acetyl-CoA Synthase:
Applications to Bacterial and Archaeal Systems
189
David
A. Grahame
1.
Introduction
190
2.
Assays of A Cluster Protein (ACS) Catalytic Activities
195
3.
Acetyltransferase Reactions
196
4.
Acetyl-CoA Synthesis Reactions
205
5.
The Acetyl-CoA CarbonykCO Exchange Reaction
211
6.
Concluding Remarks
213
Acknowledgments
214
References
215
viii Contents
и.
Acetate Kinase and Phosphotransacetylase
219
James G. Ferry
1.
Introduction
220
2.
Acetate Kinase of Methanosarcina thermophila
221
3.
Phosphotransacetylase of M. thermophila
226
Acknowledgments
229
References
229
іг.
Sodium Ion
Translocation
and ATP Synthesis in
Methanogens
233
Katharina Schlegel
and
Volker Müller
1.
introduction
234
2.
Analysis of the Na+ Dependence of Growth and Methanogenesis
237
3.
Na+ Transport in Cell Suspensions
239
4.
Determining Bioenergetic Parameters via Different
Localization of Radioactively Labeled Substances:
Intracellular Volume,
ΔρΗ,
and
ΑΨ
242
5.
ATP Synthesis
248
6.
ATP Hydrolysis
252
Acknowledgments
253
References
253
13.
Proton
Translocation
in
Methanogens
257
Cornelia
Weite
and
Uwe Deppenmeier
1.
Introduction
258
2.
Analysis of Membrane-Bound Electron Transport
in
Methanosarcina
maze i
260
3.
Measurement of Proton
Translocation
271
4.
Analysis of ATP Formation in Vesicle Preparation
276
Acknowledgments
277
References
277
14.
Measuring Isotope Fractionation by Autotrophic
Microorganisms and Enzymes
281
Kathleen M. Scott, Gordon Fox, and Peter R. Girguis
1.
Introduction
282
2.
Enzyme-Level Studies
283
3.
Culture/Cell-Level Studies
290
4.
Calculations
292
Acknowledgments
294
References
295
Contents
ЇХ
15. 2-Oxoacid
Metabolism
in Methanogenic
CoM and CoB
Biosynthesis
301
David E. Graham
1.
Introduction
302
2.
Chemistry of 2-Oxoacids
302
3.
Biosynthesis of Coenzyme
M
304
4.
Synthesis of 2-Sulfopyruvate
307
5.
Discussion of CoM Biosynthesis
308
6.
Coenzyme
В
Biosynthesis
309
7.
Homocitrate Synthase
310
8.
Homocitrate Synthase Enzymology
311
9.
Substrate Specificity Determinants in HCS
312
10.
Chemical Synthesis of 2-Oxoacids
313
11.
Chemical Synthesis of Homocitrate and Its Analogs
314
12.
Homoaconitase
315
13.
Homoaconitase Enzymology
315
14.
Chemical Synthesis of Homoaconitate and Its Analogs
316
15.
Homoisocitrate Dehydrogenase
317
16.
Enzymology of Homoisocitrate Dehydrogenase
317
17.
Synthesis of Homoisocitrate
318
18. Chromatographie
Analysis of 2-Oxoacids
318
19.
Conclusions
321
Acknowledgments
321
References
322
16.
Biomethanation and Its Potential
327
Irini Angelidaki, Dimitar Karakashev, Damien J. Batstone,
Caroline M. Plugge, and
Alfons
J. M.
Stams
1.
Introduction
328
2.
The AD Process: Microbiology and Metabolic Pathways
329
3.
Biochemical Methane Potential
338
4.
Biogas
Applications
342
5.
Biomethanation—Global Aspects
346
Acknowledgments
347
References
347
Author Index
353
Subject Index
367
|
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ctrlnum | (OCoLC)729995479 (DE-599)BVBBV037434174 |
dewey-full | 579.321 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 579 - Microorganisms, fungi & algae |
dewey-raw | 579.321 |
dewey-search | 579.321 |
dewey-sort | 3579.321 |
dewey-tens | 570 - Biology |
discipline | Biologie |
edition | 1. ed. |
format | Book |
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indexdate | 2024-12-24T01:05:03Z |
institution | BVB |
isbn | 9780123851123 |
language | English |
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physical | XLV, 373 S. Ill., graph. Darst. |
publishDate | 2011 |
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series | Methods in enzymology |
series2 | Methods in enzymology |
spellingShingle | Methods in methane metabolism Methods in enzymology |
title | Methods in methane metabolism |
title_auth | Methods in methane metabolism |
title_exact_search | Methods in methane metabolism |
title_full | Methods in methane metabolism A Methanogenesis ed. by Amy C. Rosenzweig ... |
title_fullStr | Methods in methane metabolism A Methanogenesis ed. by Amy C. Rosenzweig ... |
title_full_unstemmed | Methods in methane metabolism A Methanogenesis ed. by Amy C. Rosenzweig ... |
title_short | Methods in methane metabolism |
title_sort | methods in methane metabolism methanogenesis |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022586195&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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