Electron microscopy of model systems
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| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2010
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| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in cell biology
96 |
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| Online-Zugang: | Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a Electron microscopy of model systems |c ed. by Thomas Müller-Reichert |
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| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2010 | |
| 300 | |a XX, 724 S., [18] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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Datensatz im Suchindex
| _version_ | 1804143396057513984 |
|---|---|
| adam_text | CONTENTS
Contributors
xii
Prefaci·
xviii
Acknowledgments
xx
1.
Electron
Microscopy of Viruses
Multaci Ijiiic
1.
Introduction
2
II. Rationale
3
III. Methods
4
IV. Instrumentation and Materials
14
V. Discussion
15
References
18
2.
Bacterial
ТЕМ:
New Insights from Cryo-Microscopy
Л
/drtin
Pillwfer, Mark S. Lidinsky, Alasdair
lì .
McDowall, and
Grani J.
Jensen
I. Introduction
22
lì.
Methods Involving Dehydration and Metal Stains
24
III. Cryo-Electron Microscopy
34
References
41
3.
Analysis of the Ultrastrucrure of Archaea by Electron Microscopy
Reinhard
Rathe!,
Carolin
Meyer, Andreas
Klingi,
Sonja Cürster,
Thomas Heimerl,
Sodine
Wasserburger, Tillmann Btirghardt, Vif
Kuper,
Annett Beilack, Simone Schopf,
Reinliard
Wirth,
Harald Huber, and Gerhard Wanner
I. Introduction
48
II.
Growth of Cells for Electron Microscopy
49
III. Preparation for Transmission Electron Microscopy
51
IV. Preparation for Scanning Electron Microscopy
61
V. Instrumentation and Methods
64
VI. Conclusions
66
References
66
4.
Chlamydonwnas: Cryoprepararion Methods for the
3-D
Analysis of Cellular
Organelles
Etleen T. O Toole
I. Introduction and Historical Perspectives
72
Contents
И.
Rationale
73
III. Methods
74
IV. Instrumentation and Materials
85
V. Summary
88
References
88
5.
Ultrastructure
of the Asexual Blood Stages of Plasmodium falciparum
Eric Haussen,
Kenneth
N.
Goldie, and Leann Tilley
I. Introduction
94
II. Rationale
106
III. Methods
107
IV. Materials
Ш
References
112
6.
Electron Tomography and Immuno-labeling of Tetrahymena themiophiL·
Basal Bodies
Thomas H. Giddings Jr., Janet B. Meehl, Chad G. Pearson, and Mark Winey
I. Introduction
118
II. Rationale
122
III. Methods
123
IV. Instrumentation and Materials
133
V. Discussion
136
References
139
7.
Electron Microscopy of Paramecium (Ciliata)
Klaus
Hausmann
and Richard D. Allen
I. Introduction
143
II. Methods
145
III. Final
Remaries
171
References
171
Ultrastructural Investigation Methods for Trypanosoma brucei
Johanna L.
Höög, Eva
Gluenz, Sue Vaughan, and Keith Gull
I. Introduction
176
II. Rationale
177
HI. Methods
177
IV. Materials
191
V. Discussion
192
VI. Summary
193
References
194
Contents
Vil
9. Dictyostelium discoideutn:
A
Model System
for Ultrastructural Analyses
of
Cell Motility and Development
Michael P. Koonce and Ralph Graf
I. Introduction
198
II. Current Approaches
201
III. Future Challenges
208
IV. Summary Comments
211
References
212
10.
Toward Sub-second Correlative Light and Electron Microscopy of
Saccharomyces cerevisiae
Christopher
Buser
I. Introduction
218
II. Rationale
221
III. Methods
224
IV. Materials
225
V. Results
226
VI. Discussion
230
References
233
11.
Fission Yeast: A Cellular Model Well Suited for Electron Microscopy
Investigations
Hélio
Roque
and Claude Antony
I. Introduction
236
II. Rationale
238
III. EM Methods
239
IV. Instrumentation, Materials, and Reagents
248
V. Schizosaccharomyces pombe: Some Major Advances using EM
249
VI. Discussion and Outlook
252
VII.
Summary
255
References
· 255
12.
Electron Microscopy and High-Pressure Freezing of Arabidopsis
Byung-Ho Kang
I. Introduction
260
II. Rationale
262
III. Methods
262
IV. Materials
276
V. Discussion
278
VI. Concluding Remarks
279
References
280
Contents
13.
Preparation Techniques for Transmission Electron Microscopy of Hydra
Thomas W.
Holstein,
Michael W. Hess, and
Willi Salvenmoser
I. Introduction
286
II. Rationale
288
HI. Methods
288
IV. Materials
297
V. Results and Discussion
298
VI. Concluding Remarks
301
References
302
14.
Electron Microscopy of Flatworms: Standard and
Cryo-Preparation Methods
Willi Salvenmoser, Bernhard Egger, Johannes
G.
Achatz,
Peter Ladurner, and Michael W. Hess
I.
Introduction
308
II.
Rationale 310
III. Methods
310
IV.
Materials 322
V. Results and Discussion
323
VI. Concluding Remarks
326
References
327
15.
Three-Dimensional Reconstruction Methods for Caenorhahditis
elegans
Ultrastructure
Thomas
Müller-Reichert,
Joel Mancuso,
Ben Lieh,
and Kent McDonald
I. Introduction
332
II. Rationale
340
III. Methods
340
IV. Materials and Instrumentation
353
V. Discussion
355
References
358
16.
Insects as Model Systems in Cell Biology
Thomas A.
Keil
and R. Alexander
Steinbrecht
I. Introduction
364
II. Rationale
366
III. Methods
366
IV. Materials
378
V. Results and Discussion
379
VI. Concluding Remarks
387
VII.
Summary
388
References
388
Contents
17. Electron
Microscopy of the Amphibian Model Systems Xenopus laevis and
Ambystoma mexkanum
Thomas Kurth,
Jürgen Berger, Michaela
Wilsch-Brãunínger,
Susanne
Kretschmar,
Robert Cerny, Heinz Schwarz, Jan Lößerg, Thomas Piendl, and
Hans H. Epperlein
I.
Introduction
396
II.
Rationale 397
III. Methods
398
IV.
Materials 415
V. Discussion and
Outlook 416
References
418
18. Modern
Approaches for
Ultrastructurał
Analysis of the Zebrafish Embryo
Nicole L.
Schieber,
Sušanj.
Nixon, Richard I. Webb, Viola MJ.
Oorschot,
and
Robert G.
Parton
I. Introduction
426
II. Rationale
428
III. Methods
429
IV. Materials
437
V. Concluding Remarks and Perspectives
439
References
440
19.
Transmission Electron Microscopy of Cartilage and Bone
Douglas R. Keene and Sara F. Tufa
I. Introduction
444
II. Rationale
446
III. Methods
446
IV. Materials
468
V. Concluding Remarks
470
References
470
20.
Electron Microscopy of the Mouse Central Nervous System
Wiebke
Möbius,
Benjamin Cooper, Walter A.
Kaufmann,
Cordelia Imig,
Torben
Ruhweăel,
Nicolas
Snaiäero,
Aiman
S.
Saab, and
Frédérique Varoqueaux
I. Introduction
476
II. Rationale
479
III. Methods
480
IV. Materials
494
V. Results and Discussion
495
VI. Concluding Remarks
508
References
508
Contents
21.
Rapidly Excised and Cryofixed Rat Tissue
Dimitri
Vanhecke, Werner
Graber,
and Daniel Studer
I. Introduction
514
II. Rationale
516
III. Methods
516
IV. Materials
520
V. Results and Discussion
521
References
525
22.
The Actin Cytoskeleton in Whole Mount Preparations and Sections
Guenter P. Resell, Edit Urban, and
Sonja
Jacob
I. Introduction and Rationale
530
II. Whole Mount Techniques
531
III. Sectioning Techniques
553
IV. Summary
556
References
557
23.
Three-Dimensional Cryo-Electron Microscopy on Intermediate Filaments
Robert Kirmse, C edric Bouchet-Marquis, Cynthia Page, and Andreas Hoenger
I. Introduction
566
II. Rationale
568
III. Methods
569
IV. Materials
578
V. Discussion
581
References
586
24.
Correlative Time-Lapse Imaging and Electron Microscopy to Study
Abscission in HeLa Cells
Julien
Guizetti,
Jana Mäntler,
Thomas
Múller-Reichert,
and Daniel W. Gerlich
I. Introduction
592
II. Rationale
593
III. Methods
594
IV. Instrumentation and Materials
599
V. Discussion
600
References
601
25.
Viral Infection of Cells in Culture
—
Approaches for Electron Microscopy
Paul Walther, Li Wang, Sandra LieBem, and
Giada
Frascaroli
I. Introduction
604
II. Rationale
605
HI. Methods
606
Contents
IV.
Materials 613
V.
Discussion
615
References
617
26. Intracellular Membrane
Traffic at High Resolution
Jan R. T. van
Weering,
Edward Brown, Thomas H. Sharp, Judith
Munteli,
Veter
J. Cullen,
and Paul
Verkade
I. Abstract
620
II. Introduction
620
III. Methods
624
IV. Outlook
643
V. Materials and Equipment
644
References
645
27. 3D
Versus 2D Cell Culture: Implications for Electron Microscopy
Michael W, Hess,
Kristian
Pfaller,
Hannes L.
Ebner, Beate Beer,
Daniel
Hekl,
and Thomas
Seppi
I. Introduction
650
II. Rationale
652
III. Methods
652
IV. Materials
657
V. Results
659
VI. Discussion
662
VII.
Synopsis
666
References
667
28.
Tips and Tricks for High-Pressure Freezing of Model Systems
Kent McDonald, Heinz
Schwarz,
Thomas
Müller-Reichert, Rick
Webb,
Christopher
Buser,
and Mary Morphew
I. Introduction
672
II. Rationale
672
III. Methods
673
References
691
Subject Index
695
Volumes in Series
717
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| indexdate | 2024-07-09T22:46:59Z |
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| language | English |
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| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Electron microscopy of model systems ed. by Thomas Müller-Reichert 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2010 XX, 724 S., [18] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 96 Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Elektronenmikroskopie (DE-588)4014327-2 gnd rswk-swf Modell (DE-588)4039798-1 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Elektronenmikroskopie (DE-588)4014327-2 s Mikroorganismus (DE-588)4039226-0 s Modell (DE-588)4039798-1 s DE-604 Methode (DE-588)4038971-6 s b DE-604 Müller-Reichert, Thomas 1962- Sonstige (DE-588)132464020 oth Methods in cell biology 96 (DE-604)BV002534878 96 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020656878&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Electron microscopy of model systems Methods in cell biology Mikroorganismus (DE-588)4039226-0 gnd Elektronenmikroskopie (DE-588)4014327-2 gnd Modell (DE-588)4039798-1 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4039226-0 (DE-588)4014327-2 (DE-588)4039798-1 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Electron microscopy of model systems |
| title_auth | Electron microscopy of model systems |
| title_exact_search | Electron microscopy of model systems |
| title_full | Electron microscopy of model systems ed. by Thomas Müller-Reichert |
| title_fullStr | Electron microscopy of model systems ed. by Thomas Müller-Reichert |
| title_full_unstemmed | Electron microscopy of model systems ed. by Thomas Müller-Reichert |
| title_short | Electron microscopy of model systems |
| title_sort | electron microscopy of model systems |
| topic | Mikroorganismus (DE-588)4039226-0 gnd Elektronenmikroskopie (DE-588)4014327-2 gnd Modell (DE-588)4039798-1 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Mikroorganismus Elektronenmikroskopie Modell Methode Aufsatzsammlung |
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