RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications
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| Format: | Buch |
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| Sprache: | English |
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New York [u.a.]
Humana Press
2010
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| Schriftenreihe: | Methods in molecular biology
650 |
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| Online-Zugang: | Inhaltsverzeichnis |
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| 020 | |a 9781607617686 |c hbk. |9 978-1-60761-768-6 | ||
| 020 | |z 9781607617693 |c ebk. |9 978-1-60761-769-3 | ||
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| 245 | 1 | 0 | |a RNAi and microRNA-mediated gene regulation in stem cells |b methods, protocols, and applications |c ed. by Baohong Zhang and Edmund J. Stellwag |
| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2010 | |
| 300 | |a XI, 261 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 650 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Includes bibliographical references and index | ||
| 500 | |a RNAi in stem cells : current status and future perspectives / Gang-Ming Zou -- A brief introduction to RNAi and microRNAs in stem cells / Alexander K. Murashov -- Forward RNAi screens in human stem cells / Christine Karlsson, Jonas Larsson, and Aurélie Baudet -- A recessive genetic screen for components of the RNA interference pathway in mouse embryonic stem cells / Melanie I Trombly and Xiaozhong Wang -- Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells / Xiaoxing Cheng and Rui Jian -- Establishing efficient siRNA knockdown in stem cells using fluorescent oligonucleotides / Stephen W. Chen and Steve K.W. Oh -- Loss-of-function studies in mouse embryonic stem cells using the pHYPER shRNA plasmic vector / Soizik Berlivet, Martin Houlard, and Matthieu Gérard -- | ||
| 500 | |a Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8 / Takayuki Manabe and Akio Wanaka -- Silencing of rho-GDI[gamma] by RNAi promotes the differentiation of neural stem cells / Jiao Wang, Wei Lu, and Tieqiao Wen -- RNAi knockdown of redox signaling protein Ape1 in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Cynthia LeBron, and Yumei Fu -- Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down / Carsten Hagemann ... [et al.] -- An efficient transfection method for mouse embryonic stem cells / Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang -- Semi-quantitative analysis of transient single-cell gene expression in embryonic stem cells by femtoinjection / Mikako Saito and Hideaki Matsuoka -- | ||
| 500 | |a Preparation and analysis of microRNA libraries using the Illumiina massively parallel sequencing technology / Ryan D. Morin ... [et al.] -- Assessing in vivo microRNA function in the germline stem cells of the Drosophila ovary / Kin Chan and Hannele Ruohola-Baker -- Monitoring microRNA expression during embryonic stem-cell differentiation using quantitative real-time PCR (qRT-PCR) / Xiaoping Pan ... [et al.] -- Engineering human mesenchymal stem cells to release adenosine using miRNA technology / Gaoying Ren and Detlev Boison -- Efficient gene knockdowns in mouse embryonic stem cells using microRNA-based shRNAs / Jianlong Wang | ||
| 650 | 4 | |a Genetic regulation | |
| 650 | 4 | |a Stem cells | |
| 650 | 4 | |a MicroRNAs / Laboratory Manuals | |
| 650 | 4 | |a RNA Interference / Laboratory Manuals | |
| 650 | 4 | |a Stem Cells / Laboratory Manuals | |
| 650 | 4 | |a Gene Expression Regulation | |
| 650 | 4 | |a RNA / analysis | |
| 700 | 1 | |a Zhang, Baohong |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 650 |w (DE-604)BV035362695 |9 650 | |
| 856 | 4 | 2 | |m HEBIS Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020647408&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-020647408 | |
Datensatz im Suchindex
| _version_ | 1819726892792545280 |
|---|---|
| adam_text | IMAGE 1
RNAI AND MICRORNA-MEDIATED GENE
REGULATION IN STEM CELLS
METHODS, PROTOCOLS, AND APPLICATIONS
EDITED BY
BAOHONG ZHANG AND EDMUND J. STELLWAG
DEPARTMENT OF BIOLOGY EAST CAROLINA UNIVERSITY, GREENVILLE, NC, USA
*, $ HUMANA PRESS
IMAGE 2
CONTENTS
PREFACE V
CONTRIBUTORS IX
SECTION I INTRODUCTION
1. RNAI IN STEM CELLS: CURRENT STATUS AND FUTURE PERSPECTIVES 3
GANG-MING ZOU
2. A BRIEF INTRODUCTION TO RNAI AND MICRORNAS IN STEM CELLS 15
ALEXANDER K. MURASHOV
SECTION II RNA INTERFERENCE
3. FORWARD RNAI SCREENS IN HUMAN STEM CELLS 29
CHRISTINE KARLSSON, JONAS LARSSON, AND AURELIE BAUDET
4. A RECESSIVE GENETIC SCREEN FOR COMPONENTS OF THE RNA INTERFERENCE
PATHWAY IN MOUSE EMBRYONIC STEM CELLS 45
MELANIE I. TROMBLY AND XIAOZHONG WANG
5. CONSTRUCTION AND APPLICATION OF RANDOM DSRNA INTERFERENCE LIBRARY FOR
FUNCTIONAL GENETIC SCREENS IN EMBRYONIC STEM CELLS 65
XIAOXING CHENG AND RUIJIAN
6. ESTABLISHING EFFICIENT SIRNA KNOCKDOWN IN STEM CELLS USING
FLUORESCENT OLIGONUCLEOTIDES 75
STEPHEN W. CHEN AND STEVE K. W. OH
7. LOSS-OF-FUNCTION STUDIES IN MOUSE EMBRYONIC STEM CELLS USING THE
PHYPER SHRNA PLASMID VECTOR 85
SOIZIK BERLIVET, MARTIN HOULARD, AND MATTHIEU GERARD
8. REGULATION AND/OR REPRESSION OF CHOLINERGIC DIFFERENTIATION OF MURINE
EMBRYONIC STEM CELLS USING RNAI DIRECTED AGAINST TRANSCRIPTION FACTOR
L3/LHX8 101
TAKAYUKI MANABE AND AKIO WANAKA
9. SILENCING OF RHO-GDIY BY RNAI PROMOTES THE DIFFERENTIATION OF NEURAL
STEM CELLS I LL
JIAO WANG, WEI LU, AND TIEQIAO WEN
10. RNAI KNOCKDOWN OF REDOX SIGNALING PROTEIN APEL IN THE
DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS 121
GANG-MING ZOU, CYNTHIA LEBRON, AND TUMEI FU
VII
IMAGE 3
VIII CONTENTS
11. PROTEINS INVOLVED IN CELL MIGRATION FROM GLIOBLASTOMA NEUROSPHERES
ANALYZED BY OVEREXPRESSION AND SIRNA-MEDIATED KNOCK-DOWN 129 CARSTEN
HAGEMANN, HARUN M. SAID, MICHAEL FLENTJE, KLAUS ROOSEN, AND GILES
HAMILTON VINCE
12. AN EFFICIENT TRANSFECTION METHOD FOR MOUSE EMBRYONIC STEM CELLS 145
JUN-TANG LIOU, BOR-SHENG KO, AND TZU-CHING CHANG
13. SEMI-QUANTITATIVE ANALYSIS OF TRANSIENT SINGLE-CELL GENE EXPRESSION
IN EMBRYONIC STEM CELLS BY FEMTOINJECTION 155
MIKAKO SAITO AND HIDEAKI MATSUOKA
SECTION III MICRORNAS
14. PREPARATION AND ANALYSIS OF MICRORNA LIBRARIES USING THE ILLUMINA
MASSIVELY PARALLEL SEQUENCING TECHNOLOGY 173
RYAN D. MORIN, YONGJUN ZHAO, ANNA-LIISA PRABHU, NOREEN DHALLA, HELEN
MCDONALD, PAWAN PANDOH, ANGELA TARN, THOMAS ZENG, MARTIN HIRST, AND
MARCO MARRA
15. ASSESSING IN VIVO MICRORNA FUNCTION IN THE GERMLINC STEM CELLS OF
THE DROSOPHILA OVARY 201
KIN CHAN AND HANNELE RUOHOLA-BAKER
16. MONITORING MICRORNA EXPRESSION DURING EMBRYONIC STEM-CELL
DIFFERENTIATION USING QUANTITATIVE REAL-TIME PCR(QRT-PCR) 213 XIAOPING
PAN, ALEXANDER K. MURASHOV, EDMUND J. STELLWAG, AND BAOHONG ZHANG
17. ENGINEERING HUMAN MESENCHYMAL STEM CELLS TO RELEASE ADENOSINE USING
MIRNA TECHNOLOGY 225
GAOYING REN AND DETLEV BOISON
18. EFFICIENT GENE KNOCKDOWNS IN MOUSE EMBRYONIC STEM CELLS USING
MICRORNA-BASED SHRNAS 241
JIANLONG WANG
SUBJECT INDEX 257
|
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| id | DE-604.BV036729708 |
| illustrated | Illustrated |
| indexdate | 2024-12-24T00:12:05Z |
| institution | BVB |
| isbn | 9781607617686 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020647408 |
| oclc_num | 699852226 |
| open_access_boolean | |
| owner | DE-12 |
| owner_facet | DE-12 |
| physical | XI, 261 S. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spellingShingle | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications Methods in molecular biology Genetic regulation Stem cells MicroRNAs / Laboratory Manuals RNA Interference / Laboratory Manuals Stem Cells / Laboratory Manuals Gene Expression Regulation RNA / analysis |
| title | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications |
| title_auth | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications |
| title_exact_search | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications |
| title_full | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications ed. by Baohong Zhang and Edmund J. Stellwag |
| title_fullStr | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications ed. by Baohong Zhang and Edmund J. Stellwag |
| title_full_unstemmed | RNAi and microRNA-mediated gene regulation in stem cells methods, protocols, and applications ed. by Baohong Zhang and Edmund J. Stellwag |
| title_short | RNAi and microRNA-mediated gene regulation in stem cells |
| title_sort | rnai and microrna mediated gene regulation in stem cells methods protocols and applications |
| title_sub | methods, protocols, and applications |
| topic | Genetic regulation Stem cells MicroRNAs / Laboratory Manuals RNA Interference / Laboratory Manuals Stem Cells / Laboratory Manuals Gene Expression Regulation RNA / analysis |
| topic_facet | Genetic regulation Stem cells MicroRNAs / Laboratory Manuals RNA Interference / Laboratory Manuals Stem Cells / Laboratory Manuals Gene Expression Regulation RNA / analysis |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020647408&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
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