Lipid analysis isolation, separation, identification and lipidomic analysis
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Oily Press
2010
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24 |
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020 | |a 9780955251245 |9 978-0-9552512-4-5 | ||
020 | |a 0955251249 |9 0-9552512-4-9 | ||
035 | |a (OCoLC)699534732 | ||
035 | |a (DE-599)BVBBV036593877 | ||
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100 | 1 | |a Christie, William W. |e Verfasser |4 aut | |
245 | 1 | 0 | |a Lipid analysis |b isolation, separation, identification and lipidomic analysis |c William W. Christie and Xianlin Han |
250 | |a 4. ed. | ||
264 | 1 | |a Bridgwater |b Oily Press |c 2010 | |
300 | |a XVIII, 428 S. |b graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
490 | 1 | |a Oily Press lipid library |v 24 | |
650 | 0 | 7 | |a Lipide |0 (DE-588)4035873-2 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Analyse |0 (DE-588)4122795-5 |2 gnd |9 rswk-swf |
689 | 0 | 0 | |a Lipide |0 (DE-588)4035873-2 |D s |
689 | 0 | 1 | |a Analyse |0 (DE-588)4122795-5 |D s |
689 | 0 | |5 DE-604 | |
700 | 1 | |a Han, Xianlin |e Verfasser |4 aut | |
830 | 0 | |a Oily Press lipid library |v 24 |w (DE-604)BV012436710 |9 24 | |
856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020514529&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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adam_text | Contents
Preface
List of Abbreviations
PARTI
Introducing Lipids and their Analysis
1.
Lipids: their structures and occurrence
3
A. Introduction
3
B. Definitions
4
С
Fatty acids
5
1.
Saturated fatty acids
2.
Monoenoic fatty acids
3.
Polyunsaturated fatty acids
4.
Branched-chain fatty acids
5. Oxygenated and cyclic fatty acids
D. Principal simple lipids and complex glycerohpids of animal and
11
plant tissues
1.
Triacylglycerols and related compounds
2.
Other simple lipids
3.
Glycerophospholipids
4.
Glyceroglycolipids
5.
Ether lipids
E. Sphingolipids
16
1.
Long-chain bases
2.
Ceramides
3.
Sphingomyelin and other sphingophospholipids
4.
Neutral glycosylceramides
5.
Gangliosides
2. Chromatographie
analysis of lipids: general principles
21
A. A statement of the problem
21
B. The theory of chromatography
21
C. Modes of separation in chromatography
23
1.
Introduction
V1U CONTENTS
2.
Adsorption and normal-phase liquid-partition chromatography
3.
Gas-liquid partition chromatography
4.
Reversed-phase liquid partition chromatography
5.
Ion-exchange chromatography
6.
Silver ion chromatography
7.
Size-exclusion chromatography
8.
Chiral-phase chromatography
D. Gas chromatography
29
1.
The column and stationary phase
2.
Injection systems
3.
Detectors for gas chromatography
4.
High-temperature gas chromatography
5. Quantitative Analysis
E. High-performance liquid chromatography
34
1.
Detectors for high-performance liquid chromatography (HPLC)
2.
Injection systems
3.
Solvent pumping systems
4
Columns and fittings
5.
Mobile phases and solvent selectivity
6.
Some practical aspects
F. Thin-layer chromatography
47
1.
Procedures
2.
Quantification
3.
The Iatroscan™ analyser
G. Supercritical fluid chromatography
50
H. Mass spectrometry
51
1.
Gas chromatography-mass spectrometry (GC-MS)
2.
Liquid chromatography-mass spectrometry (LC-MS)
I. Quality control
52
3.
Lipid
extraction, storage and sample handling
55
A.
Introduction
55
B.
Some practical considerations
55
1.
Storage of tissues and preliminary treatments prior to extraction
2.
Minimizing autoxidation
3.
Storage of
lipid
extracts
4.
Contaminants and artefacts in extraction procedures
5.
Hazards
С
The principles of solvent extraction procedures
60
D.
Recommended procedures
63
CONTENTS
IX
1.
Method of Folch, Lees and Stanley
2.
Method of Bligh and Dyer
3.
Extraction of plant tissues
4.
Extraction methods for lipidomic analysis
5.
Special cases
PART
2
Chromatographie
Separation and Analysis of Individual
Lipid
Classes
4.
Analysis of simple
lipid
classes
69
A. Preliminary fractionation of
lipid
extracts
69
B. Low-pressure column chromatography
70
C. Thin-layer chromatography
71
D. High-performance liquid chromatography
73
E. Determination of
lipid
profiles by gas chromatography
76
F. Analysis of specific simple
lipid
classes
78
1.
Cholesterol and plant sterols
2.
Free (unesterified) fatty acids
3.
Diacylglycerols
4.
Alkyldiacylglycerols and neutral
plasmalogens
5.
Wax esters and other wax components
6.
Fatty alcohols
G. Oxidized fats and oils
87
1.
Determination of degree of oxidation
2. Chromatographie
analysis of oxidized triacylglycerols
5. Chromatographie
analysis of phospholipids and
91
glycosyldiacylglycerols
A. Introduction
91
B. Separation by low-pressure column chromatography
92
1.
Preparative-scale ion-exchange chromatography
2.
Solid-phase extraction chromatography
C. Thin-layer chromatography
94
1.
Introduction
2.
One-dimensional thin-layer chromatography systems
3.
Two-dimensional thin-layer chromatography systems
4.
Location and identification of complex lipids on
TLC
plates
5.
Quantification
D. High-performance liquid chromatography
103
X
CONTENTS
1. General
comments
2. Elution
procedures with UV detection
3.
Elution procedures with evaporative light-scattering detection
4.
Preparative-scale separations of phospholipids
Б.
Quantification of complex lipids separated by chromatography
117
1. Gas Chromatographie
analysis of the fatty acid constituents with
an internal standard
2.
Phosphorus determination
F. Phosphorus nuclear magnetic resonance spectroscopy
118
G. Some specific problems in the analysis of complex glycerolipids
119
1.
Alkyl-
and alkeny 1-ether forms (and derived aldehydes)
2.
Platelet-activating factor
3.
Phosphatidylinositol and polyphosphoinositides
4.
Sphingomyelin
6. Chromatographie
analysis of sphingolipids
125
A. Introduction
125
B. Preparation of sphingolipid-rich extracts
125
C. Separation of neutral glycosphingolipid classes
127
1.
Low-pressure column chromatography
2.
Thin-layer chromatography
3.
High-performance liquid chromatography
D. Separation of gangliosides
133
1.
Extraction
2.
Thin-layer chromatography
3.
High-performance liquid chromatography
E. Analysis of hydrolysis products of glycosphingolipids
136
1.
Long-chain bases
2.
Fatty acids of sphingolipids
3.
Carbohydrate and sialic acid moieties
F. Some miscellaneous glycolipids
141
1.
Glycolipid
sulfates
2.
Sphingosine
1
-phosphate
PART3
Analysis of Fatty Acids
7.
Preparation of derivatives of fatty acids
145
A. Introduction
145
B. Hydrolysis
(saponification)
of lipids
145
CONTENTS Xl
C.
Preparation of methyl and other esters of fatty acids
146
1.
Acid-catalysed esterification and transesterification
2.
Base-catalysed transesterification
3.
Diazomethane
4.
Phenacyl and related esters
5. Special cases
6.
Clean-up of methyl esters
D. Preparation of derivatives for mass spectrometry
152
1.
Pyrrolidides
2.
Picolinyl esters
3.
4,4-Dimethyloxazoline (DMOX) derivatives
E. Derivatives of double bonds
154
1.
Dimethyl disulphide addition
2.
Hydrogénation
3. Deuteration
F.
Derivatives of hydroxyl groups
156
1.
Acetylation and benzoylation
2.
Trimethylsilyl ethers and related derivatives
3.
Nicotinates
8. Gas Chromatographie
analysis of fatty acid derivatives
159
A. Introduction
159
B. Columns and liquid phases
159
C. Provisional identification using standards or retention time relationships
161
D. Separation of the common fatty acids of plants and animals
165
E. Positional and geometric isomers of unsaturated fatty acids
167
1.
Cis-
and trans-mono- and dienoic fatty acids
2.
Polyenoic fatty acids
F. Some applications to less common fatty acids
175
1.
Short-chain fatty acids
2.
Fatty acids of longer than usual chain length
3.
Branched-chain fatty acids
4.
Oxygenated fatty acids
5.
Other fatty acids
G. Quantitative estimation of fatty acid compositions
178
9.
Isolation of fatty acids and identification by
spectroscopie
181
and related techniques
A. Introduction
181
B. Simple concentration techniques
181
XU CONTENTS
1.
Urea adduct formation
2.
Partition in the form of metal ion complexes
3.
Solid-phase extraction methods
C. Silver ion chromatography
183
1.
Thin-layer chromatography
2.
High-performance liquid chromatography
3.
Solid-phase extraction and related column procedures
D. Reversed-phase high-performance liquid chromatography
189
1.
Introduction
2.
Free fatty acids and methyl esters
3.
Derivatives suitable for UV and fluorescence detection
4.
Derivatives suitable for mass spectrometry
E. Adsorption chromatography
194
F. Identification of fatty acids by chemical degradative procedures
194
1.
Chain-length determination
2.
Location of double bonds in fatty acid chains
3.
Detection and location of other functional groups in fatty acids
G. Spectroscopy of fatty acids
197
1.
Infrared spectroscopy
2.
Ultraviolet spectroscopy
3.
Nuclear magnetic resonance spectroscopy
H. Mass spectrometry
202
1.
Introduction
2.
Saturated straight-chain fatty acids
3.
Monoenoic fatty acids
4.
Di-
and polyenoic fatty acids
5. Branched-chain and cyclic fatty acids
6.
Oxygenated fatty acids
PART
4
Chromatographie
Analysis of Molecular Species of Lipids
10.
Molecular species of triacylglycerols, diacylglycerols derived
215
from complex lipids, and related lipids
A. The nature of the problem
215
B. Separation of triacylglycerol species
216
1.
Introduction and general approach
2.
Adsorption chromatography
3.
Silver ion thin-layer chromatography
4.
Silver ion high-performance liquid chromatography
5.
Reversed-phase high-performance liquid chromatography
CONTENTS
ХШ
6.
High-temperature
gas chromatography
7.
Supercritical fluid chromatography
8.
Oxidized triacylglycerols
C. Molecular species of mono-and diacylglycerols
232
1.
Introduction
2.
Generation of diacylglycerols and equivalent ether lipids from
complex lipids
3.
High-temperature gas chromatography
4.
Silver ion chromatography
5.
Reversed-phase high-performance liquid chromatography
6.
Mass spectrometry
D. Other simple lipids
243
11. Chromatographie
analysis of molecular species of intact
245
phospholipids and glycolipids
A. Introduction
245
B. High-performance liquid chromatography separation of intact
246
phospholipids
1.
Phosphatidylcholine and related lipids
2.
Phosphatidylethanolamine and other common phosphoglycerides
3.
Sphingomyelin
4.
Oxidized phospholipids
С
Glycoglycerolipids
251
1.
Reversed-phase high-performance liquid chromatography
2.
Mass spectrometry
D. Glycosphingolipids
253
1.
Ceramides (including those derived from complex sphingolipids)
2.
Glycosphingolipids in underivatized form
3.
Glycosphingolipids in derivatized form
12.
Positional distributions of fatty acids in glycerolipids
261
A. Introduction
261
B. Positional distribution of fatty acids in triacyl-sn-glycerols
261
1.
Pancreatic
lipase
hydrolysis
2.
Lipase
of Rhizopus arrhizus
3.
Chemical methods
4.
Nuclear magnetic resonance (NMR) spectroscopy
5.
Stereospecific analysis
C. Enzymatic hydrolysis of complex lipids
271
1.
Phospholipase
Кг
hydrolysis of phospholipids
2.
Glycosyldiacylglycerols
XIV CONTENTS
PARTS
Mass
Spectrometric
Analysis
of Lipids
in
Lipidomícs
13.
Introduction to mass spectrometric analysis of lipids in
277
lipidomics
A. Lipidomics
277
B
.
Brief history of mass spectrometry for
lipid
analysis
279
1.
Electron-impact and chemical ionization
2.
Atmospheric pressure chemical ionization (APCI)
3.
Fast-atom bombardment (FAB)
4.
Thermospray
ionization, field ionization, field desorption, plasma
desorption, and laser desorption
C. Modern developments in mass spectrometry for
lipid
analysis
284
1.
Matrix-assisted laser desorption/ionization (MALDI)
2.
Atmospheric pressure photoionization
3.
Electrospray ionization (ESI)
D. Tandem mass spectrometry modes useful for
lipid
analyses in lipidomics
2%
1.
Product-ion scan
2.
Precursor-ion scan
3.
Neutral loss scan
4.
Selected reaction monitoring
E. Impact of recent developments related to mass spectrometric analyses
299
of lipids in lipidomics
1.
Microfluidics for automated sample introduction into mass
spectrometers for lipidomics
2
Off-axis ion inlets for atmospheric pressure ionization
3.
Ion mobility mass spectrometry
4.
Time-of-flight secondary ion mass spectrometry
5.
Desorption electrospray ionization
14.
Characterization of lipids by electrospray ionization mass
305
spectrometry
A. Introduction
305
B. Characterization of individual classes of phospholipids
307
1.
Phosphatidylcholines and related lipids
2.
Phosphatidylethanolamines and related lipids
3.
Anionie
glycerophospholipids
4.
Cardiolipin
С
Characterization of individual classes of sphingolipids
322
1.
Sphingomyelin
CONTENTS
XV
2.
Sulfatide
3. Cerebroside
and psychosine
4. Inositol phosphoceramide
5. Ceramide
6. Sphingoid
bases and sphingoid base-1-phosphates
7. Oligoglycosylceramides and gangliosides
D.
Characterization of
simple glycerolipids and glycosyldiacylglycerols 334
1. Triacylglycerols
2. Di- and monoacylglycerols
3. Glycoglycerolipids
15.
Practical identification of
individuai
lipid species
in
lipid
339
extracts of biological samples
A. Introduction
339
1.
Instrument selection
2.
Sample size and sampling
3.
Lipid extraction for identification and quantification
4.
Sample inlets
5.
Instrument settings
B. LC-MS based identification of individual molecular species of lipid 34S
classes
C. Identification of individual molecular species of lipid classes by shotgun
349
lipidomics
1.
Introduction
2.
Multi-dimensional mass spectrometry
3.
Identification of individual species of selected lipids using multi¬
dimensional MS-based shotgun lipidomics
4.
Profiling individual species of lipids using a specific tandem mass
spectrometric analysis
5. Identification of the altered lipid species of a cellular lipidome
through differential lipid profiling
D. Location of double bonds in individual lipid species
363
16.
Quantification of lipid molecular species by electrospray
365
ionization mass spectrometry
A. Introduction
365
B. Internal standards and normalization
366
С
Quantification of lipids by HPLC-coupled ESI-MS and ESI-MS/MS
369
1.
Quantification of lipid species by HPLC-coupled ESI-MS
2.
Quantification of lipid species by HPLC-coupled ESI-MS/MS (i.e.
SRM/MRM)
XVI
CONTENTS
D.
Quantification of Upids after direct infusion
374
1.
Quantification of individual
lipid
species of a class by scanning a
characteristic fragment diagnostic of the class
2.
Quantification of individual
lipid
species by multiple or data-
dependent tandem mass spectrometry
3.
Quantification of individual
lipid
species by multi-dimensional
MS-based shotgun lipidomics
E. Concerns associated with quantification
381
1.
Quantitative analysis in the unaggregated state at which molecular
species of a polar
lipid
class have essentially identical ionization
efficiencies
2.
Ion suppression
3.
Dynamic range
4.
The impact of >3C isotopologues on quantification of individual
lipid
species
5.
The effects of baseline noise on quantification of low-abundant
lipid
species
6.
Data processing and bioinformatics
F. Conclusions
392
References
393
Index
417
LIPID
ANALYSIS
4th Edition
William W. Christie and Xianlin Han
This well-known and highly successful book was first published in
1973
and has been completely re-written in subsequent editions
(published in
1982
and
2003).
This new Fourth Edition has become
necessary because of the pace of developments in mass spectrometry
of intact lipids, which has given recognition of
lipid
analysis and
lipidomics as a distinct science. To bring the book up to date with
these developments, author William W. Christie is joined by
co-author Xianlin Han. In order to include this new material it has
been necessary to reduce substantially the amount of space devoted to
the older
Chromatographie
procedures in comparison to the Third
Edition. Nevertheless,
Lipid
Analysis remains a practical guide, in one
volume, to the complexities of the analysis of lipids. As in past
editions, it is designed to act as a primary source, of value at the
laboratory bench rather than residing on a library shelf.
The book deals with the isolation, separation, identification and
structural analysis of glycerolipids, including triacylglycerols,
phospholipids, sphingolipids, and the various hydrolysis products of
these. The chapters follow a logical sequence from the extraction of
lipids to the isolation and characterization of particular
lipid
classes
and of molecular species of each, and to the mass spectrometric
analysis of lipids in lipidomics.
The new influence of mass spectrometry is due mainly to the
development of electrospray ionization (ESI) and matrix-assisted
laser desorption/ionization (MALDI). Most emphasis in this book is
placed on ESI, which is enabling structural characterization of
different
lipid
classes and the identification of novel lipids and their
molecular species.
|
any_adam_object | 1 |
author | Christie, William W. Han, Xianlin |
author_facet | Christie, William W. Han, Xianlin |
author_role | aut aut |
author_sort | Christie, William W. |
author_variant | w w c ww wwc x h xh |
building | Verbundindex |
bvnumber | BV036593877 |
classification_rvk | VK 8520 WD 5400 |
ctrlnum | (OCoLC)699534732 (DE-599)BVBBV036593877 |
discipline | Chemie / Pharmazie Biologie |
edition | 4. ed. |
format | Book |
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id | DE-604.BV036593877 |
illustrated | Illustrated |
indexdate | 2024-12-24T00:08:35Z |
institution | BVB |
isbn | 9780955251245 0955251249 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020514529 |
oclc_num | 699534732 |
open_access_boolean | |
owner | DE-355 DE-BY-UBR DE-29T DE-20 DE-11 |
owner_facet | DE-355 DE-BY-UBR DE-29T DE-20 DE-11 |
physical | XVIII, 428 S. graph. Darst. |
publishDate | 2010 |
publishDateSearch | 2010 |
publishDateSort | 2010 |
publisher | Oily Press |
record_format | marc |
series | Oily Press lipid library |
series2 | Oily Press lipid library |
spellingShingle | Christie, William W. Han, Xianlin Lipid analysis isolation, separation, identification and lipidomic analysis Oily Press lipid library Lipide (DE-588)4035873-2 gnd Analyse (DE-588)4122795-5 gnd |
subject_GND | (DE-588)4035873-2 (DE-588)4122795-5 |
title | Lipid analysis isolation, separation, identification and lipidomic analysis |
title_auth | Lipid analysis isolation, separation, identification and lipidomic analysis |
title_exact_search | Lipid analysis isolation, separation, identification and lipidomic analysis |
title_full | Lipid analysis isolation, separation, identification and lipidomic analysis William W. Christie and Xianlin Han |
title_fullStr | Lipid analysis isolation, separation, identification and lipidomic analysis William W. Christie and Xianlin Han |
title_full_unstemmed | Lipid analysis isolation, separation, identification and lipidomic analysis William W. Christie and Xianlin Han |
title_short | Lipid analysis |
title_sort | lipid analysis isolation separation identification and lipidomic analysis |
title_sub | isolation, separation, identification and lipidomic analysis |
topic | Lipide (DE-588)4035873-2 gnd Analyse (DE-588)4122795-5 gnd |
topic_facet | Lipide Analyse |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020514529&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020514529&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV012436710 |
work_keys_str_mv | AT christiewilliamw lipidanalysisisolationseparationidentificationandlipidomicanalysis AT hanxianlin lipidanalysisisolationseparationidentificationandlipidomicanalysis |