Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2004
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| LEADER | 00000nam a2200000 c 4500 | ||
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| 001 | BV036082370 | ||
| 003 | DE-604 | ||
| 007 | t| | ||
| 008 | 100317s2004 gw ad|| m||| 00||| eng d | ||
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| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
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| 100 | 1 | |a Limmon, Gino Valentino |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |c von Gino Valentino Limmon |
| 264 | 1 | |c 2004 | |
| 300 | |a II, 87 Bl. |b Ill., graph. Darst. |c 29 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Jena, Univ., Diss., 2004 | ||
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Chlamydia pneumoniae | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018973414&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-018973414 | |
Datensatz im Suchindex
| DE-BY-UBR_call_number | 24/W16049 |
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| DE-BY-UBR_katkey | 4584017 |
| DE-BY-UBR_location | 00 |
| DE-BY-UBR_media_number | 069036648235 |
| _version_ | 1822695908921311232 |
| adam_text |
CONTENTS
ABBREVIATION
.
I
1.
INTRODUCTION
.
1
1.1.
CHLAMYDIAE
PNEUMONIAE
AN
INTRACELLULAR
P
ATHOGEN
.
1
1.1.1.
TAXONOMY
.
1
1.1.2.
PATHOGENESIS
.
2
1.1.3.
DEVELOPMENTAL
CYCLE
.
3
1.1.4.
MORPHOLOGY
AND
STRUCTURE
.
4
1.1.5.
METABOHSM
.
5
1.2.
CHLAMYDIAE
AND
HOST
CELL
INTERACTION
.
6
1.2.1.
ATTACHMENT
AND
ENTRY
.
6
1.2.2.
SIGNAL
TRANSDUCTION
.
7
1.2.3.
ACTIVATION
OF
IMMUNE
RESPONSES
(INFLAMMATION)
.
8
1.2.4.
APOPTOSIS
.
9
1.3.
ASSOCIATION
BETWEEN
CHLAMYDIA
PNEUMONIAE
INFECTION
AND
ATHEROSCLEROSIS
.
11
1.4.
MICROARRAY
.
13
2.
MATERIALS
AND
METHODS
.
15
2.1.
MATERIALS
.
15
2.1.1.
CELL
LINES
.
15
2.1.1.1.
EPITHELIAL
CELLS
(HEP-2)
.
15
2.1.1.2.
FIBROBLAST
CELLS
.
15
2.1.1.3.
HUMAN
UMBILICAL
VEIN
ENDOTHELIAL
CELLS
(HUVEC).
15
2.1.2.
MEDIUM,
BUFFERS
AND
SOLUTIONS
.
15
2.1.3.
CHEMICALS
.
17
2.1.4.
ENZYMES
AND
ANTIBODIES
.
18
2.1.5.
KITS
AND
DEVICES
.
18
2.1.6.
SOFTWARE
.
19
2.2.
METHODS
.
20
2.2.1.
EXPERIMENTAL
SETUP
.
20
2.2.2.
MICROARRAY
(CHIP)
CONSTRUCTION
.
20
2.2.2.1.
PRESCREENING
(FILTER
HYBRIDIZATION)
.
20
2.2.2.2.
PROBE
PREPARATION,
SPOTTING,
AND
POST
PROCESSING
OF
ARRAY
.
21
2.2.3.
TARGET
PREPARATION
AND
HYBRIDIZATION
.
23
2.2.3.1.
CELL
CULTURE
.
23
2.2.3.2.
INFECTION
OF
CELL
LINES
WITH
CHLAMYDIA
PNEUMONIAE
.
23
2.2.4.
REVERSE
TRANSCRIPTION,
LABELLING,
HYBRIDIZATION
AND
WASHING.
24
2.2.5.
DATA
ANALYSIS
.
25
2.2.5.1.
SCANNING
AND
RAW
DATA
EXTRACTION
.
25
2.2.5.2.
DATA
NORMAHZATION,
THRESHOLDING
AND
FILTERING
.
26
2.2.6.
REAL
TIME
PCR
.
26
2.2.7.
APOPTOSIS
ASSAYS
.
28
2.2.8.
WESTERN
BLOT
EXPERIMENT
.
28
3.
RESULTS
.
30
3.1.
TECHNICAL
PART
.
30
3.1.1.
MICROARRAY
.
30
3.1.1.1.
PRESCREENING
(FILTER
HYBRIDIZATION)
.
30
3.1.1.2.
PROBE
PREPARATION
.
31
3.1.1.2.1.
AMPLIFICATION
AND
PURIFICATION
OF
PCR
PRODUCTS
.
31
3.1.1.2.2.
IMMOBILIZATION
OF
PROBES
AND
POST
PROCESSING
OF
ARRAY.
32
3.1.1.3.
TARGET
PREPARATION
.
34
3.1.1.3.1.
CELL
CULTURE
AN
INFECTION
OF
CELL
LINES
WITH
CHLAMYDIA
PNEUMONIAE
.
34
3.1.1.3.2.
TOTAL
RNA
AND
MRNA
EXTRACTION
.
35
3.1.1.3.3.
REVERSE
TRANSCRIPTION
AND
LABELLING
.
36
3.1.1.4.
HYBRIDIZATION
.
37
3.1.1.5.
DATA
ANALYSIS.
39
3.1.1.5.1.
NORMALIZATION
STRATEGY
.
39
3.1.1.5.2.
CLUSTER
ANALYSIS
.
42
3.2.
BIOLOGICAL
PART
.
45
3.2.1.
PRO
AND
ANTI
INFLAMMATORY
CYTOKINES.
48
3.2.1.1.
CHEMOKINES
AND
CHEMOKINE
RECEPTORS
.
48
3.2.1.2.
INTERLEUKINS
AND
INTERLEUKIN
RECEPTORS
.
49
3.2.1.3.
INTERFERONS
AND
INTERFERON
RECEPTORS
.
50
3.2.1.4.
TUMOUR
NECROSIS
FACTOR
AND
TNF
RECEPTORS
.
51
3.2.2.
SIGNAL
TRANSDUCTION
.
52
3.2.3.
CELL
CYCLE
.
53
3.2.4.
APOPTOSIS
.
55
3.2.5.
REAL
TIME
PCR
.
55
3.2.6.
APOPTOSIS
ASSAYS.
59
3.2.7.
WESTERN
BLOT
EXPERIMENT
.
61
4.
DISCUSSION
.
64
4.1.
GENE
EXPRESSION
PROFILE
.
64
4.2.
ACTIVATION
OF
IMMUNE
REACTION
.
65
4.3.
ACTIVATION
OF
SIGNAL
TRANSDUCTION
.
69
4.4.
CELL
CYCLE
.
70
4.5.
APOPTOSIS
.
71
REFERENCES
.
76
ZUSAMMENFASSUNG
SELBSTANDIGKEITSERKLARUNG |
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| author | Limmon, Gino Valentino |
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| illustrated | Illustrated |
| indexdate | 2025-01-02T15:11:06Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018973414 |
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| physical | II, 87 Bl. Ill., graph. Darst. 29 cm |
| publishDate | 2004 |
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| publishDateSort | 2004 |
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| spellingShingle | Limmon, Gino Valentino Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae Apoptosis Chlamydia pneumoniae |
| subject_GND | (DE-588)4113937-9 |
| title | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_auth | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_exact_search | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_full | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon |
| title_fullStr | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon |
| title_full_unstemmed | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon |
| title_short | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_sort | gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria chlamydia pneumoniae |
| topic | Apoptosis Chlamydia pneumoniae |
| topic_facet | Apoptosis Chlamydia pneumoniae Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018973414&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT limmonginovalentino geneexpressionprofileandmechanismofapoptosisinhibitioninhostcellsinfectedbyintracellularbacteriachlamydiapneumoniae |