Helicases methods and protocols

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Veröffentlicht: New York Humana Press 2010
Schriftenreihe:Methods in molecular biology 587
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adam_text Titel: Helicases Autor: Abdelhaleem, Mohamed M. Jahr: 2010 Contents Preface. v Contributors........................................................ tx 1. Helicases: An Overview.............................................. 1 Mohamed Abdelhaleem 2. Fluorescent Biosensors to Investigate Helicase Activity..................... 13 Martin R. Webb 3. Single-Molecule FRET Analysis of Helicase Functions...................... 29 Eli Rothenberg and Taekjip Ha 4. Kinetics of Motor Protein Translocation on Single-Stranded DNA............ 45 Christopher J. Fischer, Lake Wooten, Eric J. Tomko, and Timothy M. Lohman 5. Experimental and Computational Analysis of DNA Unwinding and Polymerization Kinetics.......................................................... 57 Manjula Pandey, Mikhail K. Levin, and Smita S. Paul 6. Protein Displacement by Helicases..................................... 85 Laxmi Yeruva and Kevin D. Raney 7. In Vivo Investigation of Protein-Protein Interactions tor Helicases Using Tandem Affinity Purification.......................................... 99 Matthew Jessulat, Terry Buist, MdAlamgir, Mohsen Hooshyar, Jianhua Xu, Hiroyuki Aoki, M. Clelia Ganoza, Gareth Butland, and Ashkan Golshani 8. Mapping Genomic Targets of DNA Helicases by Chromatin Immunoprecipitation in Saccharomyces cerevisiae.......................... 113 Jennifer Cobb and Haico van Attikum 9. Methods to Study How Replication Fork Helicases Unwind DNA............ 127 Daniel L. Kaplan and Irina Brack 10. Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription Termination Factor Rho from Escherichia colt............................. 137 Marc Boudvillain, Celine Walmacq, Annie Schwartz, and Frederique Jacquinot 11. Single-Molecule Studies of RecBCD.................................... 155 Thomas T. Perkins and Hung-Wen Li 12. Mutational Analysis of Bloom Helicase.................................. 173 Xu Guang Xi 13. In Vivo Analysis of Drosophila BLM Helicase Function During DNA Double-Strand Gap Repair........................................... 185 Mitch McVey 14. Purification and Characterization of RecQ Helicases of Plants................ 195 Daniela Kobbe, Manfred Focke, and Holger Puchta VII viii Contents 15. Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity............... 211 Mariusz Krawczyk, Anna Stankiewicz-Drogon, Anne-Lise Haenni, and Anna Boguszewska-Chachulska 16. A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase and Protease Activities.............................................. 223 David N. Prick, Olya Ginzburg, and Angela M.I. Lam 17. Computer Modeling of Helicases Using Elastic Network Model.............. 235 Wenjun Zheng 18. Duplex Unwinding with DEAD-Box Proteins............................ 245 Eckhard Jankowsky and Andrea Putnam 19. Analysis of the RNA Helicase p68 (Ddx5) as a Transcriptional Regulator....... 265 Samantha M. Nicol and Frances V. Fuller-Pace 20. A Method to Study the Role of DDX3 RNA Helicase in HIV-1 .............. 281 Chia-Yen Chen, VenkatR.K. Yedavalli, and Kuan-Teh Jeang 21. Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A)..... 291 Suisheng Zhang and Frank Grosse 22. Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins by MLE.......................................................... 303 Hyangyee Oh, Andrew M. Parrott, Yongkyu Park, and Chee-Gun Lee 23. Biochemical Characterization of Human Upfl Helicase..................... 327 Zhihong Cheng, Gaku Morisawa, and Haiwei Song 24. Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome......................................... 339 Michal Malecki, Piotr P. Stepien, and Pawel Golik 25. Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast Piflp In Vitro..................................................... 359 Jean-Baptiste Boule and Virginia A. Zakian 26. A Method to Confer Salinity Stress Tolerance to Plants by Helicase Overexpression.................................................... 377 Narendra Tuteja 27. A Method to Inhibit the Growth of Plasmodium falciparum by Double-Stranded RNA-Mediated Gene Silencing of Helicases.............................. 389 Renu Tuteja Subject Index.......................................................... 401
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physical XI, 404 S. Ill., graph. Darst.
publishDate 2010
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series Methods in molecular biology
series2 Methods in molecular biology
Springer protocols
spelling Helicases methods and protocols ed. by Mohamed M. Abdelhaleem
New York Humana Press 2010
XI, 404 S. Ill., graph. Darst.
txt rdacontent
n rdamedia
nc rdacarrier
Methods in molecular biology 587
Springer protocols
Includes bibliographical references and index
DNA helicases
DNA helicases - LCSH
Helicasen (DE-588)4159496-4 gnd rswk-swf
Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf
(DE-588)4143413-4 Aufsatzsammlung gnd-content
Helicasen (DE-588)4159496-4 s
Untersuchungsmethode (DE-588)4324907-3 s
DE-604
Haleem, M. A. Sonstige oth
Methods in molecular biology 587 (DE-604)BV035362695 587
HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018972047&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle Helicases methods and protocols
Methods in molecular biology
DNA helicases
DNA helicases - LCSH
Helicasen (DE-588)4159496-4 gnd
Untersuchungsmethode (DE-588)4324907-3 gnd
subject_GND (DE-588)4159496-4
(DE-588)4324907-3
(DE-588)4143413-4
title Helicases methods and protocols
title_auth Helicases methods and protocols
title_exact_search Helicases methods and protocols
title_full Helicases methods and protocols ed. by Mohamed M. Abdelhaleem
title_fullStr Helicases methods and protocols ed. by Mohamed M. Abdelhaleem
title_full_unstemmed Helicases methods and protocols ed. by Mohamed M. Abdelhaleem
title_short Helicases
title_sort helicases methods and protocols
title_sub methods and protocols
topic DNA helicases
DNA helicases - LCSH
Helicasen (DE-588)4159496-4 gnd
Untersuchungsmethode (DE-588)4324907-3 gnd
topic_facet DNA helicases
DNA helicases - LCSH
Helicasen
Untersuchungsmethode
Aufsatzsammlung
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018972047&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link (DE-604)BV035362695
work_keys_str_mv AT haleemma helicasesmethodsandprotocols