Helicases methods and protocols
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Sprache: | English |
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New York
Humana Press
2010
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Schriftenreihe: | Methods in molecular biology
587 |
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020 | |a 9781603273541 |9 978-1-60327-354-1 | ||
035 | |a (OCoLC)644063806 | ||
035 | |a (DE-599)GBV616134487 | ||
040 | |a DE-604 |b ger | ||
041 | 0 | |a eng | |
049 | |a DE-12 | ||
245 | 1 | 0 | |a Helicases |b methods and protocols |c ed. by Mohamed M. Abdelhaleem |
264 | 1 | |a New York |b Humana Press |c 2010 | |
300 | |a XI, 404 S. |b Ill., graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
490 | 1 | |a Methods in molecular biology |v 587 | |
490 | 0 | |a Springer protocols | |
500 | |a Includes bibliographical references and index | ||
650 | 0 | |a DNA helicases | |
650 | 4 | |a DNA helicases - LCSH | |
650 | 0 | 7 | |a Helicasen |0 (DE-588)4159496-4 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Untersuchungsmethode |0 (DE-588)4324907-3 |2 gnd |9 rswk-swf |
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999 | |a oai:aleph.bib-bvb.de:BVB01-018972047 |
Datensatz im Suchindex
_version_ | 1804141138286739456 |
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adam_text | Titel: Helicases
Autor: Abdelhaleem, Mohamed M.
Jahr: 2010
Contents
Preface.
v
Contributors........................................................ tx
1. Helicases: An Overview.............................................. 1
Mohamed Abdelhaleem
2. Fluorescent Biosensors to Investigate Helicase Activity..................... 13
Martin R. Webb
3. Single-Molecule FRET Analysis of Helicase Functions...................... 29
Eli Rothenberg and Taekjip Ha
4. Kinetics of Motor Protein Translocation on Single-Stranded DNA............ 45
Christopher J. Fischer, Lake Wooten, Eric J. Tomko, and Timothy M. Lohman
5. Experimental and Computational Analysis of DNA Unwinding and Polymerization
Kinetics.......................................................... 57
Manjula Pandey, Mikhail K. Levin, and Smita S. Paul
6. Protein Displacement by Helicases..................................... 85
Laxmi Yeruva and Kevin D. Raney
7. In Vivo Investigation of Protein-Protein Interactions tor Helicases Using
Tandem Affinity Purification.......................................... 99
Matthew Jessulat, Terry Buist, MdAlamgir, Mohsen Hooshyar, Jianhua Xu,
Hiroyuki Aoki, M. Clelia Ganoza, Gareth Butland, and Ashkan Golshani
8. Mapping Genomic Targets of DNA Helicases by Chromatin
Immunoprecipitation in Saccharomyces cerevisiae.......................... 113
Jennifer Cobb and Haico van Attikum
9. Methods to Study How Replication Fork Helicases Unwind DNA............ 127
Daniel L. Kaplan and Irina Brack
10. Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription
Termination Factor Rho from Escherichia colt............................. 137
Marc Boudvillain, Celine Walmacq, Annie Schwartz,
and Frederique Jacquinot
11. Single-Molecule Studies of RecBCD.................................... 155
Thomas T. Perkins and Hung-Wen Li
12. Mutational Analysis of Bloom Helicase.................................. 173
Xu Guang Xi
13. In Vivo Analysis of Drosophila BLM Helicase Function During DNA
Double-Strand Gap Repair........................................... 185
Mitch McVey
14. Purification and Characterization of RecQ Helicases of Plants................ 195
Daniela Kobbe, Manfred Focke, and Holger Puchta
VII
viii Contents
15. Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity............... 211
Mariusz Krawczyk, Anna Stankiewicz-Drogon, Anne-Lise Haenni,
and Anna Boguszewska-Chachulska
16. A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase
and Protease Activities.............................................. 223
David N. Prick, Olya Ginzburg, and Angela M.I. Lam
17. Computer Modeling of Helicases Using Elastic Network Model.............. 235
Wenjun Zheng
18. Duplex Unwinding with DEAD-Box Proteins............................ 245
Eckhard Jankowsky and Andrea Putnam
19. Analysis of the RNA Helicase p68 (Ddx5) as a Transcriptional Regulator....... 265
Samantha M. Nicol and Frances V. Fuller-Pace
20. A Method to Study the Role of DDX3 RNA Helicase in HIV-1 .............. 281
Chia-Yen Chen, VenkatR.K. Yedavalli, and Kuan-Teh Jeang
21. Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A)..... 291
Suisheng Zhang and Frank Grosse
22. Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins
by MLE.......................................................... 303
Hyangyee Oh, Andrew M. Parrott, Yongkyu Park, and Chee-Gun Lee
23. Biochemical Characterization of Human Upfl Helicase..................... 327
Zhihong Cheng, Gaku Morisawa, and Haiwei Song
24. Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast
Mitochondrial Degradosome......................................... 339
Michal Malecki, Piotr P. Stepien, and Pawel Golik
25. Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast
Piflp In Vitro..................................................... 359
Jean-Baptiste Boule and Virginia A. Zakian
26. A Method to Confer Salinity Stress Tolerance to Plants by Helicase
Overexpression.................................................... 377
Narendra Tuteja
27. A Method to Inhibit the Growth of Plasmodium falciparum by Double-Stranded
RNA-Mediated Gene Silencing of Helicases.............................. 389
Renu Tuteja
Subject Index.......................................................... 401
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genre_facet | Aufsatzsammlung |
id | DE-604.BV036080971 |
illustrated | Illustrated |
indexdate | 2024-07-09T22:11:06Z |
institution | BVB |
isbn | 9781603273541 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018972047 |
oclc_num | 644063806 |
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physical | XI, 404 S. Ill., graph. Darst. |
publishDate | 2010 |
publishDateSearch | 2010 |
publishDateSort | 2010 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spelling | Helicases methods and protocols ed. by Mohamed M. Abdelhaleem New York Humana Press 2010 XI, 404 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 587 Springer protocols Includes bibliographical references and index DNA helicases DNA helicases - LCSH Helicasen (DE-588)4159496-4 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Helicasen (DE-588)4159496-4 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Haleem, M. A. Sonstige oth Methods in molecular biology 587 (DE-604)BV035362695 587 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018972047&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Helicases methods and protocols Methods in molecular biology DNA helicases DNA helicases - LCSH Helicasen (DE-588)4159496-4 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
subject_GND | (DE-588)4159496-4 (DE-588)4324907-3 (DE-588)4143413-4 |
title | Helicases methods and protocols |
title_auth | Helicases methods and protocols |
title_exact_search | Helicases methods and protocols |
title_full | Helicases methods and protocols ed. by Mohamed M. Abdelhaleem |
title_fullStr | Helicases methods and protocols ed. by Mohamed M. Abdelhaleem |
title_full_unstemmed | Helicases methods and protocols ed. by Mohamed M. Abdelhaleem |
title_short | Helicases |
title_sort | helicases methods and protocols |
title_sub | methods and protocols |
topic | DNA helicases DNA helicases - LCSH Helicasen (DE-588)4159496-4 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
topic_facet | DNA helicases DNA helicases - LCSH Helicasen Untersuchungsmethode Aufsatzsammlung |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018972047&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT haleemma helicasesmethodsandprotocols |