Methods in modern biophysics
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Heidelberg [u.a.]
Springer
2009
|
| Ausgabe: | 3. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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| 020 | |a 9783642030215 |9 978-3-642-03021-5 | ||
| 020 | |a 9783642030222 |9 978-3-642-03022-2 | ||
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| 100 | 1 | |a Nölting, Bengt |d 1962- |e Verfasser |0 (DE-588)113522673 |4 aut | |
| 245 | 1 | 0 | |a Methods in modern biophysics |c Bengt Nölting |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Heidelberg [u.a.] |b Springer |c 2009 | |
| 300 | |a XVI, 273 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Biophysics | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biophysik |0 (DE-588)4006891-2 |2 gnd |9 rswk-swf |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-017988510 | ||
Datensatz im Suchindex
| DE-BY-TUM_call_number | 0302/PHY 820f 2005 A 8239(3) |
|---|---|
| DE-BY-TUM_katkey | 1717112 |
| DE-BY-TUM_media_number | 040030234489 |
| _version_ | 1816713028229398528 |
| adam_text | Contents
1
The three-dimensional structure of proteins
.......................................................1
1.1
Structure of the native state
.......................................................................1
1.2
Protein folding transition states
.................................................................9
1.3
Structural determinants of the folding rate constants
..............................12
1.4
Support of structure determination by protein folding simulations
........20
2
Liquid chromatography of biomolecules
.........................................................23
2.1
Ion exchange chromatography
................................................................23
2.2
Gel filtration chromatography
................................................................28
2.3
Affinity chromatography
........................................................................31
2.4
Counter-current chromatography and ultrafi ltration
...............................33
3
Mass spectrometry
...........................................................................................37
3.1
Principles of operation and types of spectrometers
................................37
3.1.1
Sector mass spectrometer
................................................................38
3.1.2
Quadrupole mass spectrometer
.......................................................39
3.1.3
Ion trap mass spectrometer
..............................................................39
.4
Time-of-flight mass spectrometer
....................................................40
.5
Fourier transform mass spectrometer
..............................................43
.6
Ionization, ion transport and ion detection
......................................44
.7
Ion fragmentation
............................................................................45
.8
Combination with
Chromatographie
methods
.................................46
3.2
Biophysical applications
.........................................................................49
4
X-ray structural analysis
..................................................................................59
4.1
Fourier transform and X-ray crystallography
.........................................59
4.1.1
Fourier transform
............................................................................59
4.1.2
Protein X-ray crystallography
.........................................................69
4.1.2.1
Overview
..................................................................................69
4.1.2.2
Production of suitable crystals
.................................................69
4.1.2.3
Acquisition of the diffraction pattern
.......................................71
XII Contents
4.1.2.4 Determination
of the
phases:
heavy atom replacement
............76
4.1.2.5
Calculation of the electron density and refinement
.................83
4.1.2.6
Cryocrystallography and time-resolved crystallography
.........84
4.2
X-ray scattering
.......................................................................................
°J
4.2.1
Small angle X-ray scattering (SAXS)
.............................................85
4.2.2
X-ray backscattering
........................................................................
88
5
Protein infrared spectroscopy
..........................................................................91
5.1
Spectrometers and devices
..................................................................... 2
5.1.1
Scanning infrared spectrometers
......................................................92
5.1.2
Fourier transform infrared (FTIR) spectrometers
............................92
5.1.3
LIDAR,
optical coherence tomography, attenuated total
................96
reflection and
IR
microscopes
5.2
Applications
..........................................................................................
Ю2
6
Electron microscopy
...................................................................................... 07
6.1
Transmission electron microscope
(ТЕМ)
...........................................107
6.1.1
General design
...............................................................................
Ю7
6.1.2
Resolution
......................................................................................109
6.1.3
Electron sources
............................................................................1
Ю
6.1.4
ТЕМ
grids
......................................................................................112
6.1.5
Electron lenses
..............................................................................112
6.1.6
Electron-sample interactions and electron spectroscopy
...............115
6.1.7
Examples of biophysical applications
...........................................117
6.2
Scanning transmission electron microscope (STEM)
...........................119
7
Scanning probe microscopy
...........................................................................121
7.1
Atomic force microscope (AFM)
..........................................................121
7.2
Scanning tunneling microscope (STM)
................................................133
7.3
Scanning nearfield optical microscope
(SNOM)
..................................135
7.3.1
Overcoming the classical limits of optics
......................................135
7.3.2
Design ofthesubwavelength aperture
..........................................138
7.3.3
Examples of
SNOM
applications
.................................................142
7.4
Scanning ion conductance microscope, scanning thermal
....................
I43
microscope and further scanning probe microscopes
8
Biophysical nanotechnology
..........................................................................147
8.1
Force measurements in single protein molecules
..................................
I47
8.2
Force measurements in a single polymerase-DNA complex
................150
Contents
XIII
8.3
Molecular
recognition
...........................................................................152
8.4
Protein nanoarrays and protein engineering
.........................................155
8.5
Study and manipulation of protein crystal growth
...............................158
8.6
Nanopipettes, molecular diodes, self-assembled nanotransistors,
........159
nanoparticle-mediated transfection, and further biophysical
nanotechnologies
9
Proteomics: high throughput protein functional analysis
..............................165
9.1
Target discovery
...................................................................................166
9.2
Interaction proteomics
..........................................................................168
9.3
Chemical proteomics
............................................................................. 172
9.4
Lab-on-a-chip technology, mass-spectrometric array scanners,
.........173
and robots
9.5
Structural proteomics
...........................................................................175
10
Ion mobility spectrometry
...........................................................................177
10.1
General design of spectrometers
........................................................177
10.2
Resolution and sensitivity
...................................................................182
10.3
IMS-based sniffers
..........................................................................185
10.4
Design details
......................................................................................186
10.5
Detection of biological agents
............................................................195
11
^Value analysis
......................................................................................... 199
11.1
The method
......................................................................................... 199
11.2
High resolution of six protein folding transition states
......................201
12
Evolutionary computer programming
.........................................................205
12.1
Reasons for the necessity of self-evolving computer programs
..........205
12.2
General features of the method
...........................................................205
12.3
Protein folding and structure simulations
...........................................208
12.4
Evolution of nanooptical devices made from nanoparticles
..............209
12.4.1
Materials and methods
................................................................209
12.4.2
Results and discussion
................................................................210
12.5
Further potential applications
............................................................212
13
Rapid partial protein ladder sequencing
.....................................................215
13.1
Principle of operation
.........................................................................215
13.2
An example: myoglobin
.....................................................................216
XIV Contents
13.3 Potential
experimental
problems
........................................................221
14
Surface labeling analysis of protein interactions
.........................................223
14.1
Principle of operation
.........................................................................223
14.2
An example: NHS-biotin label
...........................................................224
14.3
Potential experimental problems
........................................................226
15
Conclusions
.................................................................................................229
16
References
...................................................................................................231
Index
..................................................................................................................263
|
| any_adam_object | 1 |
| author | Nölting, Bengt 1962- |
| author_GND | (DE-588)113522673 |
| author_facet | Nölting, Bengt 1962- |
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| ctrlnum | (OCoLC)467742559 (DE-599)BVBBV035711588 |
| dewey-full | 571.4 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.4 |
| dewey-search | 571.4 |
| dewey-sort | 3571.4 |
| dewey-tens | 570 - Biology |
| discipline | Physik Biologie Chemie |
| edition | 3. ed. |
| format | Book |
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| id | DE-604.BV035711588 |
| illustrated | Illustrated |
| indexdate | 2024-11-25T17:26:05Z |
| institution | BVB |
| isbn | 9783642030215 9783642030222 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017988510 |
| oclc_num | 467742559 |
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| owner_facet | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-11 DE-1102 DE-703 DE-634 |
| physical | XVI, 273 S. graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Springer |
| record_format | marc |
| spellingShingle | Nölting, Bengt 1962- Methods in modern biophysics Biophysics Methode (DE-588)4038971-6 gnd Biophysik (DE-588)4006891-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4006891-2 |
| title | Methods in modern biophysics |
| title_auth | Methods in modern biophysics |
| title_exact_search | Methods in modern biophysics |
| title_full | Methods in modern biophysics Bengt Nölting |
| title_fullStr | Methods in modern biophysics Bengt Nölting |
| title_full_unstemmed | Methods in modern biophysics Bengt Nölting |
| title_short | Methods in modern biophysics |
| title_sort | methods in modern biophysics |
| topic | Biophysics Methode (DE-588)4038971-6 gnd Biophysik (DE-588)4006891-2 gnd |
| topic_facet | Biophysics Methode Biophysik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017988510&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT noltingbengt methodsinmodernbiophysics |