Bacteriophages methods and protocols 2 Molecular and applied aspects
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| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2009
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| Schriftenreihe: | Methods in molecular biology
502 |
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| Online-Zugang: | Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a Bacteriophages |b methods and protocols |n 2 |p Molecular and applied aspects |c ed. by Martha R.J. Clokie and Andrew M. Kropinski |
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Datensatz im Suchindex
| _version_ | 1819721543722205184 |
|---|---|
| adam_text | Contents
Preface j?
Contributors ix
Introduction xiii
Section I: Bacteriophage Genomics
1 Preparation of Bacteriophage Lysates and Pure DNA
Derek John Juan Pickard 3
2 Approaches to the Compositional Analysis of DNA
Richard A. Mandervitte and Andrew M. Kropinski 11
3 Determination of Bacteriophage Genome Size by Pulsed-Field Gel
Electrophoresis
Erika Lingohr, Shelley Frost and Roger P. Johnson 19
4 Preparation of a Phage DNA Fragment Library for Whole Genome
Shotgun Sequencing
Elizabeth J. Summer 27
5 PCR and Partial Sequencing of Bacteriophage Genomes
Martha Clokie 47
6 In Silico Identification of Genes in Bacteriophage DNA
Andrew M. Kropinski, Mark Borodovsky, TimJ. Carver,
Ana M. Cerdeno-Tdrraga, Aaron Darling, Alexandre Lomsadze,
Padmanabhan Mahadevan, Paul Stothard, Donald Seto,
Gary Van Domselaar and David S. Wishart 57
7 Determining DNA Packaging Strategy by Analysis of the Termini of the
Chromosomes in Tailed-Bacteriophage Virions
Sherwood R. Casjens and Eddie B. Gilcrease 91
8 In silico Characterization of DNA Motifs with Particular Reference
to Promoters and Terminators
Rob Lavigne, Andre Villegas, Andrew M. Kropinksi 113
9 Molecular Phylogenetics: Testing Evolutionary Hypotheses
David A. Walsh and Adrian K. Sharma 131
Section II: Bacteriophage Transcriptomics and Proteomics
10 Preparation of RNA from Bacteria Infected with Bacteriophages: A Case
Study from the Marine Unicellular Synechococcus sp. WH7803 Infected
by Phage S-PM2
Jinyu Shan and Martha Clokie 171
vii
viii Contents
11 Quantification of Host and Phage mRNA Expression During Infection
Using Real-Time PCR
Dr Martha R.J. Clokie 177
12 Oligonucleotide Microarrays for Bacteriophage Expression Studies
Andrew D. Millard and Beta Tiwari 193
13 Purification of Bacteriophages and SDS-PAGE Analysis of Phage
Structural Proteins from Ghost Particles
Pascale Boulanger 227
14 Phage Proteomics: Applications of Mass Spectrometry
Rob Lavigne, Pieter-Jan Ceyssens and]. Robben 239
Section III: Community Bacteriophage Approaches
15 Isolation Independent Methods of Characterizing Phage Communities 1:
Strain Typing Using Fingerprinting Methods
Clemens Pausz, Jessica L. Clasen and Curtis A. Suttle 255
16 Isolation Independent Methods of Characterizing Phage Communities 2:
Characterizing a Metagenome
K. Eric Wommack, Shellie R. Bench, Jaysheel Bhavsar, David Mead,
and Tom Hanson 279
Section IV: Applied Aspects of Bacteriophage Biology
17 Phage Typing
Irina Chirakadze, Ann Perets and Rafiq Ahmed 293
18 A Genetic Screen to Identify Bacteriophage Lysins
Raymond Schuch, Vincent A. Fischetti, and Daniel C. Nelson 307
19 General M13 Phage Display: M13 Phage Display in Identification
and Characterization of Protein-Protein Interactions
Kirsten Hertveldt, Tim Belien, and Guido Volckaert 321
20 Isolation of Monoclonal Antibody Fragments from Phage Display
Libraries
Mehdi Arbabi-Ghahroudi, Jamshid Tanha and Roger MacKenzie 341
21 Internet Resources of Interest to Bacteriophage Workers
Andrew M. Kropinski 365
Index 371
|
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| illustrated | Illustrated |
| indexdate | 2024-12-23T21:27:06Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017149908 |
| oclc_num | 310754373 |
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| physical | XXII, 373 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | Bacteriophages methods and protocols Methods in molecular biology Bacteriophages Bacteriophages Research Methodology |
| title | Bacteriophages methods and protocols |
| title_auth | Bacteriophages methods and protocols |
| title_exact_search | Bacteriophages methods and protocols |
| title_full | Bacteriophages methods and protocols 2 Molecular and applied aspects ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| title_fullStr | Bacteriophages methods and protocols 2 Molecular and applied aspects ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| title_full_unstemmed | Bacteriophages methods and protocols 2 Molecular and applied aspects ed. by Martha R.J. Clokie and Andrew M. Kropinski |
| title_short | Bacteriophages |
| title_sort | bacteriophages methods and protocols molecular and applied aspects |
| title_sub | methods and protocols |
| topic | Bacteriophages Bacteriophages Research Methodology |
| topic_facet | Bacteriophages Bacteriophages Research Methodology |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017149908&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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