Kidney research experimental protocols

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Sprache:English
Veröffentlicht: Totowa, N.J. Humana 2009
Schriftenreihe:Methods in molecular biology 466
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MARC

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Datensatz im Suchindex

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adam_text Contents Preface ............................................................................................................... v Contributors ..................................................................................................... ix List of Color Plates ........................................................................................... xiii Part I Isolation and Propagation of Cell Populations 1 Isolation and Propagation of Glomerular Mesangial Cells .................... 1 Paolo Mené and Antonella Stoppacciaro 2 Isolation and Primary Culture of Human Proximal Tubule Cells ........ 19 David A. Vesey, Weier Qi, Xinming Chen, Carol A. Pollock, and David W. Johnson 3 Propagation and Culture of Renal Fibroblasts ....................................... 25 Lauren Grimwood and Rosemary Masterson Part II Animal Models 4 Small Animal Models of Kidney Disease: A Review ............................... 39 Tim D. Hewitson, Takahiko Ono, and Gavin J. Becker Part HI Techniques and Applications 5 Measurement of Glomerular Filtration Rate in Conscious Mice .......... 59 Zhonghua Qi and Matthew D. Breyer 6 Laser Capture Microdissection of Kidney Tissue ................................... 73 Robert P. Woroniecki and Erwin P. Bottinger 7 Quantitative Gene Expression Analysis in Kidney Tissues .................... 83 Chris Tikellis, Philip Koh, Wendy Burns, and Phillip Kantharidis viii Contents 8 In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy ...................................................... 109 A. Richard Kitching, Michael P. Kuligowski, and Michael J. Hickey 9 Using In Situ Hybridization to Localize Renal Gene Expression in Tissue Sections ..................................................................................... 119 Ian A. Darby, Alexis Desmoulière, and Tim D. Hewitson 10 Immune and Lectin Histochemistry for Renal Light Microscopy ...... 133 Tim D. Hewitson and Lauren Grimwood 11 Immune and Lectin Histochemistry for Renal Electron Microscopy ................................................................................ 149 Mitsuru Nakajima 12 Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia ................................... 161 Christian Rosenberger, Seymour Rosen, Alexander Paliege, and Samuel N. Heyman 13 Identification of Apoptosis in Kidney Tissue Sections .......................... 175 Glenda Gobe 14 Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction .................................. 193 Kristen J. Kelynack 15 Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells .................................................................... 205 Joan Krepinsky 16 Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue .............................................................. 223 Chrishan S. Samuel 17 SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue ....................................................................................... 237 Eleni Giannakis, Chrishan S. Samuel, Wee-Ming Boon, Mary Macris, Tim D. Hewitson, and John D. Wade 18 In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli ....................................................................... 251 Yoshitsugu Takabatake, Yoshitaka Isaka, and Enyu Imai Index .................................................................................................................. 265
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physical XV, 268 S. Ill., graph. Darst. 25 cm
publishDate 2009
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publisher Humana
record_format marc
series Methods in molecular biology
series2 Methods in molecular biology
spellingShingle Kidney research experimental protocols
Methods in molecular biology
Kidneys / Diseases
Kidney / physiopathology
Kidney Diseases
Maladies du rein - physiopathologie - Cahiers de laboratoire
Rein - Maladies ram
Techniques et procédures de laboratoire - Cahiers de laboratoire
Kidney Diseases physiopathology Laboratory Manuals
Kidneys Diseases
Laboratory Techniques and Procedures Laboratory Manuals
Cytologie (DE-588)4070177-3 gnd
Labortechnik (DE-588)4123602-6 gnd
Niere (DE-588)4042270-7 gnd
subject_GND (DE-588)4070177-3
(DE-588)4123602-6
(DE-588)4042270-7
(DE-588)4143413-4
title Kidney research experimental protocols
title_auth Kidney research experimental protocols
title_exact_search Kidney research experimental protocols
title_full Kidney research experimental protocols ed. by Tim D. Hewitson ...
title_fullStr Kidney research experimental protocols ed. by Tim D. Hewitson ...
title_full_unstemmed Kidney research experimental protocols ed. by Tim D. Hewitson ...
title_short Kidney research
title_sort kidney research experimental protocols
title_sub experimental protocols
topic Kidneys / Diseases
Kidney / physiopathology
Kidney Diseases
Maladies du rein - physiopathologie - Cahiers de laboratoire
Rein - Maladies ram
Techniques et procédures de laboratoire - Cahiers de laboratoire
Kidney Diseases physiopathology Laboratory Manuals
Kidneys Diseases
Laboratory Techniques and Procedures Laboratory Manuals
Cytologie (DE-588)4070177-3 gnd
Labortechnik (DE-588)4123602-6 gnd
Niere (DE-588)4042270-7 gnd
topic_facet Kidneys / Diseases
Kidney / physiopathology
Kidney Diseases
Maladies du rein - physiopathologie - Cahiers de laboratoire
Rein - Maladies
Techniques et procédures de laboratoire - Cahiers de laboratoire
Kidney Diseases physiopathology Laboratory Manuals
Kidneys Diseases
Laboratory Techniques and Procedures Laboratory Manuals
Cytologie
Labortechnik
Niere
Aufsatzsammlung
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017043760&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link (DE-604)BV035362695
work_keys_str_mv AT hewitsontimd kidneyresearchexperimentalprotocols