Angiogenesis in vivo systems A In vivo systems, part A

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Veröffentlicht: Amsterdam [u.a.] Elsevier, Acad. Press 2008
Schriftenreihe:Methods in enzymology 444
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adam_text Contents Contributors xi Preface xv Volumes in Series xvii 1. Molecular Mechanism of Type IV Collagen-Derived Endogenous Inhibitors of Angiogenesis 1 Vesselina G. Cooke and Raghu Kalluri 1. Introduction 2 2. Molecular Mechanism of Type IV Collagen-Derived Endogenous Inhibitors 3 3. Assessing the Role of Type IV Collagen-Derived Endogenous Inhibitor During Angiogenesis 13 4. Concluding Remarks 16 References 17 2. Chick Embryo Chorioallantoic Membrane Models to Quantify Angiogenesis Induced by Inflammatory and Tumor Cells or Purified Effector Molecules 21 Elena I. Deryugina and James P. Quigley 1. Introduction 22 2. Overview of CAM Angiogenesis Models 24 3. Assessing the Role of Purified Effector Molecules and Cells in Angiogenesis Using CAM Collagen Onplant Model 30 4. CAM Collagen Onplant Assay Protocol 31 5. Validation of CAM Angiogenesis Findings in Collagen Implant Mouse Model 38 Acknowledgment 39 References 39 3. The Adenoviral Vector Angiogenesis/Lymphangiogenesis Assay 43 Janice A. Nagy, Shou-Chmg Shih, Wendy H. Wong, Ann M. Dvorak, and Harold F. Dvorak 1. Introduction 44 2. Methods 46 v¡ Contents 3. Blood Vessels Induced by Ad-VEGF-A164 53 4. Giant Lymphatics Induced by Ad-VEGF-A164 58 5. Use of Ear Angiogenesis Assay to Evaluate Antiangiogenesis Drugs 59 6. Commentary and Future Perspectives 60 Acknowledgments 61 References 61 H. Using the Zebrafish to Study Vessel Formation 65 Mary С McKinney and Brant M. Weinstein 1. Introduction 66 2. Methods for Functional Manipulation of Zebrafish 68 3. Methods for Visualizing Vessels in Zebrafish 76 4. Conclusion 91 Acknowledgment 91 References 91 5. Evaluating Vascular Leak In Vivo 99 Sara M. Weis 1. Introduction 100 2. Mechanisms of Vascular Leak 100 3. Consequences of Vascular Leak in Disease 101 4. Models to Study Vascular Leak In Vivo 102 5. Concluding Remarks 112 Acknowledgments 112 References 112 6. Ocular Models of Angiogenesis 115 Edith Aguilar, Michael 1. Dorrell, David Friedlander, Ruth A. Jacobson, Audra Johnson, Valentina Marchetti, Stacey K. Moreno, Matthew R. Ritter, and Martin Friedlander 1. Introduction 116 2. Techniques Commonly Used in Ocular Angiogenesis Protocols 118 3. Corneal Micropocket Assay 120 4. Neonatal Mouse Retinal Developmental Angiogenesis Model 124 5. Oxygen-Induced Retinopathy Model 128 6. Laser-induced Retinal and Choroidal Neovascularization Models 135 7. Retinal Vascular Permeability Model 138 8. Three-Dimensional In Vivo Imaging of the Mouse Ocular Vasculature 141 9. Cell-based Models and Ocular Angiogenesis 151 10. Concluding Remarks 154 References 154 Contents vii 7. Mouse Models of Ischemie Angiogenesis and Ischemia- Reperfusion Injury 159 Joshua I. Greenberg, Ahmed Suliman, Samuel Barillas, and Niren Angle 1. Introduction 160 2. Overview of Methods in Hindlimb I/R Injury 161 3. Evaluating Hindlimb Ischemia/Reperfusion Injury 164 4. Overview of Methods in Chronic Hindlimb Ischemia 167 5. Overview of Murine Models of Acute Myocardial Infarction and Myocardial I/R Injury 171 6. Conclusion 173 References 174 8. Noninvasive Imaging of Blood Vessels 175 Milan Makale 1. Introduction 176 2. Methods 181 References 197 9. Intravital Videomicroscopy in Angiogenesis Research 201 lan C MacDonald and Ann F. Chambers 1. Introduction 202 2. Methods 206 3. Summary 228 Acknowledgments 228 References 228 10. In Vivo Measurements of Blood Flow and Glial Cell Function with Two-Photon Laser-Scanning Microscopy 231 Fritjof Helmchen and David Kleinfeld 1. Introduction 232 2. Two-Photon Microscopy of Fluorescent Labels as a Tool for Brain Imaging 234 3. Photoprocesses for Targeted Disruption of Vascular Flow 247 4. Outlook 248 Acknowledgments 251 References 251 v¡¡¡ Contents 11. The Role of Bone Marrow-Derived Cells in Tumor Angiogenesis and Metastatic Progression 255 Marianna Papaspyridonos and David Lyden 1. The Angiogenic Switch 256 2. Origin of Angiogenic Endothelial Cells 256 3. Bone-Marrow-Derived Cells: Endothelial and Hematopoietic Progenitor Cells 257 4. Bone Marrow-Cell Mobilization in Response to Tumor Factors 258 5. Contribution of BMDCs in Tumor Angiogenesis 258 6. EPCs and H PCs are Required for Tumor Angiogenesis 259 7. Cooperation between BM-Derived Hematopoietic and Endothelial Precursors 260 8. VEGFR1+ HPCs Define the Premetastatic Niche 261 9. Stromal Cells: Carcinoma-Associated Fibroblasts (CAFs) 263 10. Activation of the SDF-1-CXCR4 Pathway in Mobilization of Proangiogenic Cells 263 11. Future Directions 265 References 266 12. Structure of Microvascular Networks in Genetic Hypertension 271 Walter L. Murfee and Geert W. Schmid-Schönbein 1. Introduction 272 2. Methods 273 3. Results 275 4. Discussion 279 5. Summary 282 References 282 13. Oxygen as a Direct and Indirect Biological Determinant in the Vasculature 285 Yan Huang and Frank J. Giordano 1. Introduction 286 2. Mechanisms of Oxygen Sensing and Transduction into Vascular Biological Responses 286 3. Physiological, Pathophysiological, and Clinical Settings in Which Oxygen Levels Influence the Vasculature 293 4. Summary 300 References 300 Contents IX 14. Measuring Intratumoral Microvessel Density 305 Noel Weidner 1. Introduction 305 2. Measuring Intratumoral Microvessel Density 307 3. Highlighting Microvessels for Microvessel Counting 310 4. Histological Quantitäten of Tumor Angiogenesis 312 5. Novel In Vivo Methods for Measuring Microvessel Density 314 References 315 Author Index 325 Subject Index 353
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publishDate 2008
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series Methods in enzymology
series2 Methods in enzymology
spellingShingle Angiogenesis in vivo systems
Methods in enzymology
title Angiogenesis in vivo systems
title_auth Angiogenesis in vivo systems
title_exact_search Angiogenesis in vivo systems
title_full Angiogenesis in vivo systems A In vivo systems, part A ed. by David A. Cheresh
title_fullStr Angiogenesis in vivo systems A In vivo systems, part A ed. by David A. Cheresh
title_full_unstemmed Angiogenesis in vivo systems A In vivo systems, part A ed. by David A. Cheresh
title_short Angiogenesis
title_sort angiogenesis in vivo systems in vivo systems part a
title_sub in vivo systems
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