Plant signal transduction methods and protocols

This text provides well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in plant analyses and the proteins involved in signal transduction.

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Sprache:English
Veröffentlicht: New York, NY Humana Press 2009
Schriftenreihe:Methods in molecular biology 479
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Datensatz im Suchindex

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adam_text Contents Preface .......................................................... v Contributors ...................................................... їх 1 Multiple Dimensions in Plant Signal Transduction: An Overview .......... 1 ]orge ƒ. Casal 2 Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions ....................................... 17 Cordelia Bolle 3 Phenotyping of Abiotic Responses and Hormone Treatments m Arabidopsis .................................................... 35 Cordelia Bolle 4 Accurate Real-time Reverse Transcription Quantitative PCR .............. 61 Marco Klatte and Petra Bauer 5 Probing Spatio-temporal Intracellular Calcium Variations in Plants ......... 79 Axel Mith ofer, Christian Mazars, and Massimo E. Maffei 6 Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy ................. 93 Andreas J. Meyer and Thorsten Brach 7 Imaging of Reactive Oxygen Species in vivo ............................ 109 Steven M. Driev er, Michael f. Fryer, Philip M. Mullineaux, and Neil R. Baker 8 Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single Cysteine Mutant-immobilized Resin ........... 117 Ken Motohashi, Patrick G. N. Romano, and Toru Hisabori 9 Determination of m vivo Protein Phosphorylation in Photosynthetic Membranes .................................... 133 Julia P. Vamonen, Alexander V. Vener, and Eva-Man Aro 10 Examining Protein Stability and Its Relevance for Plant Growth and Development ................................ 147 Claus Schwechheimer, Björn C. Willige, Melma Zourelidou, and Esther M. N. Dohmann 11 Probing Circadian Rhythms in Chlamydomonas rhemhardtü by Functional Proteomics ........................................ 173 Volker Wagner and Maria Mittag 12 Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells ......... 189 Katia Schütze, Klaus Harter, and Christina Chah an 13 Fluorescence Cross-Correlation Spectroscopy of Plant Proteins ........... 203 Hideki Muto, Masataka Ktnjo, and Kotaro T. Yamamoto VII viii Contents 14 The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin-system (mbSUS) .................. 217 Christopher Grefen, Petr Obrdlik, and Klaus Harter 15 Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways ................................. 235 Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil, and Markus Teige 16 Phosphatase Activities Analyzed by m vivo Expressions .................. 247 Alois Scbweijjhofer, Zahra Ayatollahi, and Irute Meskiene 17. Chromatm Immunoprecipitation Experiments to Investigate in vivo Binding of Arabidopsis Transcription Factors to Target Sequences ...................................... 261 Ъепщтт Fode and Christiane Gatz 18 Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins ............................ 273 Sebastian Sterner and Thomas Pfannschmidt 19 Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay ................................... 291 Dierk Wanke and Klaus Harter 20 Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Arded Sequence Evaluation .............................. 311 Kenneth W. Berendzen, Klaus Harter, and Dierk Wanke 21 RNA-protein Interaction Mediating Post-transcnptional Regulation in the Orcadian System ......................................... 337 Jan C. Schoning and Dorothée Staiger Index ............................................................... 353
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publishDate 2009
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series Methods in molecular biology
series2 Methods in molecular biology
Springer-Protocols
spellingShingle Plant signal transduction methods and protocols
Methods in molecular biology
Transduction du signal cellulaire chez les plantes ram
Transduction du signal
Gene Expression Regulation, Plant
Plant Physiological Phenomena
Plant cellular signal transduction
Plant molecular biology
Plants growth & development
Signal Transduction
Labortechnik (DE-588)4123602-6 gnd
Signaltransduktion (DE-588)4318717-1 gnd
Pflanzen (DE-588)4045539-7 gnd
subject_GND (DE-588)4123602-6
(DE-588)4318717-1
(DE-588)4045539-7
(DE-588)4143413-4
title Plant signal transduction methods and protocols
title_auth Plant signal transduction methods and protocols
title_exact_search Plant signal transduction methods and protocols
title_full Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt
title_fullStr Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt
title_full_unstemmed Plant signal transduction methods and protocols ed. by Thomas Pfannschmidt
title_short Plant signal transduction
title_sort plant signal transduction methods and protocols
title_sub methods and protocols
topic Transduction du signal cellulaire chez les plantes ram
Transduction du signal
Gene Expression Regulation, Plant
Plant Physiological Phenomena
Plant cellular signal transduction
Plant molecular biology
Plants growth & development
Signal Transduction
Labortechnik (DE-588)4123602-6 gnd
Signaltransduktion (DE-588)4318717-1 gnd
Pflanzen (DE-588)4045539-7 gnd
topic_facet Transduction du signal cellulaire chez les plantes
Transduction du signal
Gene Expression Regulation, Plant
Plant Physiological Phenomena
Plant cellular signal transduction
Plant molecular biology
Plants growth & development
Signal Transduction
Labortechnik
Signaltransduktion
Pflanzen
Aufsatzsammlung
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volume_link (DE-604)BV035362695
work_keys_str_mv AT pfannschmidtthomas plantsignaltransductionmethodsandprotocols