Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Cuvillier
2007
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| Ausgabe: | 1. Aufl. |
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| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV026606259 | ||
| 003 | DE-604 | ||
| 005 | 20110228 | ||
| 007 | t | ||
| 008 | 110326s2007 ad|| m||| 00||| eng d | ||
| 015 | |a 07,N15,0895 |2 dnb | ||
| 015 | |a 07,A43,0881 |2 dnb | ||
| 016 | 7 | |a 983499268 |2 DE-101 | |
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| 020 | |a 3867271682 |9 3-86727-168-2 | ||
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| 100 | 0 | |a Aung Aung |d 1970- |e Verfasser |0 (DE-588)133139735 |4 aut | |
| 245 | 1 | 0 | |a Feeding of Leucaena mimosine on small ruminants |b investigation on the control of its toxicity in small ruminants |c submitted by Aung Aung |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Göttingen |b Cuvillier |c 2007 | |
| 300 | |a X, 137 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Zugl.: Göttingen, Univ., Diss., 2007 | ||
| 650 | 7 | |a Goats |2 cabt | |
| 650 | 7 | |a Sheep |2 cabt | |
| 650 | 7 | |a Leucaena leucocephala |2 cabt | |
| 650 | 7 | |a Mimosine |2 cabt | |
| 650 | 7 | |a Toxicity |2 cabt | |
| 650 | 7 | |a Animal Feeding |2 cabt | |
| 650 | 7 | |a Microbial Flora |2 cabt | |
| 650 | 7 | |a Degradation |2 cabt | |
| 650 | 0 | 7 | |a Toxizität |0 (DE-588)4078372-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Friedländer-Bakterium |0 (DE-588)4155451-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Schaf |0 (DE-588)4051990-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Wilde Tamarinde |0 (DE-588)4135912-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Futtermittel |0 (DE-588)4019014-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Schaf |0 (DE-588)4051990-9 |D s |
| 689 | 0 | 1 | |a Futtermittel |0 (DE-588)4019014-6 |D s |
| 689 | 0 | 2 | |a Wilde Tamarinde |0 (DE-588)4135912-4 |D s |
| 689 | 0 | 3 | |a Toxizität |0 (DE-588)4078372-8 |D s |
| 689 | 0 | 4 | |a Friedländer-Bakterium |0 (DE-588)4155451-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022161466&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-022161466 | ||
Datensatz im Suchindex
| _version_ | 1804145204368769024 |
|---|---|
| adam_text | Contents
Page
Table of contents i
Abbreviation vi
List of tables viii
List of figures X
1 Introduction 1
2 Literature Review 3
2.1 Mimosine 3
2.1.1 Mimosine in Leucaena 3
2.2 Leucaena leucocephala (leucaena) 4
2.2.1 Description 5
2.2.2 Some common names of Leucaena leucocephala 6
2.2.3 Environmental adaptation 6
2.3 Nutritive value of Leucaena 7
2.3.1 Leaves 7
2.3.2 Seeds 11
2.4 Use of Leucaena in livestock production 12
2.4.1 Leucaena as cattle feed 12
2.4.2 Leucaena as sheep feed 13
2.4.3 Leucaena as goat feed 14
2.4.4 Leucaena in poultry ration 15
2.4.5 Leucaena as feed for other animals 16
2.5 Adverse effects of Leucaena Mimosine on livestock 16
production
2.5.1 Cattle 16
2.5.2 Sheep 18
2.5.3 Goat 18
2.5.4 Poultry 19
2.5.5 Rats and mice 20
2.6 Acceptable daHy intake of Mimosine for the animals 21
2.7 Some metabolic pathways of Mimosine toxicity 21
2.8 Possible solutions to alleviate Mimosine toxicity 25
2.8.1 Development of Mimosine low cultivars 25
2.8.2 Heat treatment to the Leucaena leaves 26
2.8.3 Water washing, boiling or soaking 27
2.8.4 Detoxifying Mimosine with mineral supplementation 27
2.8.5 Control of Mimosine toxicity in ruminants by using 28
ruminal microbes
2.8.5.1 Natural distribution of the Mimosine 29
detoxifying ruminal bacteria
2.8.5.2 Persistence of ruminal 3,4-DHP degrading 30
bacteria
2.8.5.3 Isolation and characterization of Mimosine 31
detoxifying bacteria
2.8.5.4 Detection of Mimosine detoxifying ruminal 31
bacteria
2.8.5.5 Ruminal inoculation methods 34
2.8.5.6 Production of bacterial culture containing S. 34
jonesii
Selection, isolation, characterization and detection of 36
properties of Mimosine degrading ruminal bacteria from
German steers
3.1 Materials 36
3.2 Methods 36
3.2.1 Fermenter System 36
3.2.1.1 Fermenter vessel 37
3.2.1.2 Medium supply 38
3.2.1.3 Treatment with mimosine 38
3.2.1.4 Control of incubation temperature and pH 38
3.2.1.5 Out let of the overflow of fermenter fluid 38
3.2.1.6 Fermentation process 39
3.3 Detection of Mimosine degradability 39
3.3.1 Colourimetric assay 40
3.3.2 Preparation of momosine standard curve 40
3.4 Isolation and characterisation of Mimosine degrading 40
ruminal bacteria
3.4.1 Bacteriological staining 40
3.4.2 DNA isolation 41
3.4.3 PCR amplication process (16SPCR) 41
3.4.4 Purification of PCR fragments for sequencing 42
3.4.5 Gene sequencing 42
3.5 Comparison of some physical properties, morphology of 43
colonies and biochemical properties of isolate 3948
with reference strains
3.5.1 Morphology of colonies 43
3.5.2 Bacterial mobility 43
3.5.3 Capsule staining 44
3.5.4 Tests for biochemical properties 44
3.5.4.1 Microbial community analysis test (Biolog) 44
3.5.4.2 API test 45
3.6 Results 45
3.6.1 Standard curve for Mimosine content in rumen juice 45
3.6.2 Mimosine degradation 46
3.6.3 Microscopic appearance of isolate 3948 48
3.6.4 Comparison of some properties of isolate 3948 with 49
reference strains
3.6.4.1 Physical properties 49
3.6.4.2 Biochemical properties 49
3.7 Discussion 54
3.7.1 Mimosine degradation 54
3.7.2 Identification of isolate 3948 56
3.7.3 Dominant phenotype of Mimosine degrading ruminal 57
bacteria
3.8 Conclusion 58
4 Feeding trials 59
4.1 Multiplication of Mimosine degrading bacteria using IBT- 59
Gottinger Bioreactor
4.1.1 Materials 59
4.1.2 Fermenter vessel 61
4.1.3 Medium supply 62
4.1.4 Inoculum preparation 62
4.1.5 Control of incubation temperature and pH 62
4.1.6 Gaseous exchange system 62
4.1.7 Air filters 63
4.1.8 Sampling and harvesting system 63
4.1.9 Counting bacteria by using most probable number 63
(MPN)
4.2 Preservation of fermenter suspension for field experiment 64
4.2.1 Entrapment of bacteria in alginate beads 64
4.2.1.1 Counting bacteria after entrapment 65
4.2.2 Freeze-drying and counting the cells after freeze- 65
drying
4.3 Digestion trial in Myanmar sheep 66
4.3.1 Materials and methods 66
4.3.1.1 Source of rice straw 66
4.3.1.2 Manipulation of feedstuffs 66
4.3.1.3 Preliminary experiment to estimate toxic level 67
of leucaena
4.3.1.4 Experimental animals, management of animals 67
and experimental period
4.3.1.5 Experimental design, period and sample 67
collection
69
69
70
70
70
4.3.2 Observations
4.3.3 Chemical analysis
3.3.4 Statistical analysis
4.4 Results
4.4.1 Bacterial cell counts
4.4.1.1 After production 70
4.4.1.2 In alginate beads 70
4.4.1.3 After freeze-drying 70
4.4.2 Dry matter intake and toxic level of Leucaena in 70
Myanmar sheep
4.4.3 Clinical symptoms of Leucaena toxicosis 71
4.4.4 Chemical composition of feedstuffs and diets 71
4.4.5 Voluntary feed intake 72
4.4.6 Digestibilities of nutrients and nutritive values of diets 73
4.4.7 Daily nutrient intakes and digestible nutrient intakes 75
4.4.8 Nitrogen utilization 78
4.5 Discussion 80
4.5.1 Toxic symptoms of Mimosine 80
4.5.2 Chemical composition of Leucaena and diets 81
4.5.3 Nutrient intakes and nitrogen retention 82
4.5.4 Correspondence of Mimosine on digestibility of nutrients 85
4.6 Conclusion 87
5 General discussion 88
5.1 Some probiotic effects of K. pneumoniae 89
5.2 Effect of infusion of preserved Mimosine degrading ruminal 90
bacteria on Mimosine detoxification
6 Conclusion 93
7 Summary 94
7 Zusammenfassung 96
8 References 98
Appendices 123
Acknowledgement 136
|
| any_adam_object | 1 |
| author | Aung Aung 1970- |
| author_GND | (DE-588)133139735 |
| author_facet | Aung Aung 1970- |
| author_role | aut |
| author_sort | Aung Aung 1970- |
| author_variant | a a aa |
| building | Verbundindex |
| bvnumber | BV026606259 |
| ctrlnum | (OCoLC)184698071 (DE-599)DNB983499268 |
| dewey-full | 636.30855 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.30855 |
| dewey-search | 636.30855 |
| dewey-sort | 3636.30855 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Biologie Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| id | DE-604.BV026606259 |
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| indexdate | 2024-07-09T23:15:44Z |
| institution | BVB |
| isbn | 9783867271684 3867271682 |
| language | English |
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| physical | X, 137 S. Ill., graph. Darst. |
| publishDate | 2007 |
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| spelling | Aung Aung 1970- Verfasser (DE-588)133139735 aut Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants submitted by Aung Aung 1. Aufl. Göttingen Cuvillier 2007 X, 137 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Göttingen, Univ., Diss., 2007 Goats cabt Sheep cabt Leucaena leucocephala cabt Mimosine cabt Toxicity cabt Animal Feeding cabt Microbial Flora cabt Degradation cabt Toxizität (DE-588)4078372-8 gnd rswk-swf Friedländer-Bakterium (DE-588)4155451-6 gnd rswk-swf Schaf (DE-588)4051990-9 gnd rswk-swf Wilde Tamarinde (DE-588)4135912-4 gnd rswk-swf Futtermittel (DE-588)4019014-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Schaf (DE-588)4051990-9 s Futtermittel (DE-588)4019014-6 s Wilde Tamarinde (DE-588)4135912-4 s Toxizität (DE-588)4078372-8 s Friedländer-Bakterium (DE-588)4155451-6 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022161466&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Aung Aung 1970- Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants Goats cabt Sheep cabt Leucaena leucocephala cabt Mimosine cabt Toxicity cabt Animal Feeding cabt Microbial Flora cabt Degradation cabt Toxizität (DE-588)4078372-8 gnd Friedländer-Bakterium (DE-588)4155451-6 gnd Schaf (DE-588)4051990-9 gnd Wilde Tamarinde (DE-588)4135912-4 gnd Futtermittel (DE-588)4019014-6 gnd |
| subject_GND | (DE-588)4078372-8 (DE-588)4155451-6 (DE-588)4051990-9 (DE-588)4135912-4 (DE-588)4019014-6 (DE-588)4113937-9 |
| title | Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants |
| title_auth | Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants |
| title_exact_search | Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants |
| title_full | Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants submitted by Aung Aung |
| title_fullStr | Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants submitted by Aung Aung |
| title_full_unstemmed | Feeding of Leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants submitted by Aung Aung |
| title_short | Feeding of Leucaena mimosine on small ruminants |
| title_sort | feeding of leucaena mimosine on small ruminants investigation on the control of its toxicity in small ruminants |
| title_sub | investigation on the control of its toxicity in small ruminants |
| topic | Goats cabt Sheep cabt Leucaena leucocephala cabt Mimosine cabt Toxicity cabt Animal Feeding cabt Microbial Flora cabt Degradation cabt Toxizität (DE-588)4078372-8 gnd Friedländer-Bakterium (DE-588)4155451-6 gnd Schaf (DE-588)4051990-9 gnd Wilde Tamarinde (DE-588)4135912-4 gnd Futtermittel (DE-588)4019014-6 gnd |
| topic_facet | Goats Sheep Leucaena leucocephala Mimosine Toxicity Animal Feeding Microbial Flora Degradation Toxizität Friedländer-Bakterium Schaf Wilde Tamarinde Futtermittel Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022161466&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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