Affinity chromatography methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ [u.a.]
Humana Press
2008
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
421 |
| Schlagworte: | |
| Online-Zugang: | Publisher description Table of contents only Inhaltsverzeichnis |
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| 007 | t| | ||
| 008 | 080520s2008 xxuad|| |||| 00||| eng d | ||
| 010 | |a 2007930114 | ||
| 020 | |a 9781588296597 |c alk. paper |9 978-1-58829-659-7 | ||
| 020 | |a 9781597455824 |9 978-1-59745-582-4 | ||
| 035 | |a (OCoLC)191317697 | ||
| 035 | |a (DE-599)BVBBV023307889 | ||
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| 041 | 0 | |a eng | |
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| 245 | 1 | 0 | |a Affinity chromatography |b methods and protocols |c ed. by Michael Zachariou |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ [u.a.] |b Humana Press |c 2008 | |
| 300 | |a XII, 343 S. |b Ill., graph. Darst. |c 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 421 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Cromatografia |2 larpcal | |
| 650 | 4 | |a Affinity chromatography |v Laboratory manuals | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Affinitätschromatographie |0 (DE-588)4211538-3 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Affinitätschromatographie |0 (DE-588)4211538-3 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Zachariou, Michael |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 421 |w (DE-604)BV035362695 |9 421 | |
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| 856 | 4 | |u http://www.loc.gov/catdir/enhancements/fy0826/2007930114-t.html |3 Table of contents only | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-016492220 | |
Datensatz im Suchindex
| DE-19_call_number | 1801/VG 7600 B158(2) |
|---|---|
| DE-19_location | 85 |
| DE-BY-UBM_katkey | 3885707 |
| DE-BY-UBM_media_number | 41624538020014 |
| _version_ | 1823054001338318848 |
| adam_text | Contents
Preface vi i
Contributors xi
1. Affinity Chromatography: History, Perspectives, Limitations
and Prospects 1
Ana Cecilia A. Roque and Christopher R. Lowe
Part I: Various Modes of Affinity Chromatography
2. Immobilized Metal Ion Affinity Chromatography
of Native Proteins 25
Adam Charlton and Michael Zachariou
3. Affinity Precipitation of Proteins Using Metal Chelates 37
Ashok Kumar, Igor Yu. Galaev, and Bo Mattiasson
4. Immunoaffinity Chromatography 53
Stuart R. Gallant, Vish Koppaka, and Nick Zecherle
5. Dye Ligand Chromatography 61
Stuart R. Gallant, Vish Koppaka, and Nick Zecherle
6. Purification of Proteins Using Displacement Chromatography 71
Nihal Tugcu
Part II: Affinity Chromatocraphy Using
Purification Tags
7. Rationally Designed Ligands for Use in Affinity Chromatography:
An Artificial Protein L 93
Ana Cecilia A. Roque and Christopher R. Lowe
8. Phage Display of Peptides in Ligand Selection for Use in Affinity
Chromatography 111
Joanne L. Casey, Andrew M. Coley, and Michael Foley
9. Preparation, Analysis and Use of an Affinity Adsorbent
for the Purification of GST Fusion Protein 125
Gareth M. Forde
ix
x Contents
10. Immobilized Metal Ion Affinity Chromatography
of Histidine-Tagged Fusion Proteins 137
Adam Charlton and Michael Zachariou
11. Methods for the Purification of HQ-Tagged Proteins 151
Becky Godat, Laurie Engel, Natalie A. Betz,
and Tonny M. Johnson
12. Amylose Affinity Chromatography of Maltose-Binding Protein:
Purification by both Native and Novel Matrix-Assisted Dialysis
Refolding Methods 169
Leonard K. Pattenden and Walter G. Thomas
13. Methods for Detection of Protein-Protein
and Protein-DNA Interactions Using HaloTag™ 191
Marjeta Urh, Danette Hartzell, Jacqui Mendez,
Dieter H. Klaubert, and Keith Wood
14. Site-Specific Cleavage of Fusion Proteins 211
Adam Charlton
15. The Use of TAGZyme for the Efficient Removal of N-Terminal
H is-Tags 229
Jose Arnau, Conni Lauritzen, Gitte Ebert Petersen,
and John Pedersen
Part III: Various Applications of Affinity
Chromatography
16. Affinity Processing of Cell-Containing Feeds Using Monolithic
Macroporous Hydrogels, Cryogels 247
Igor Yu. Galaev and Bo Mattiasson
17. Monolithic Bioreactors for Macromolecules 257
Mojca Bencina, Katja Bencina, Ales Podgornik,
and Ales Strancar
18. Plasmid DNA Purification Via the Use of a Dual
Affinity Protein 275
Gareth M. Forde
19. Affinity Chromatography of Phosphorylated Proteins 285
Grigoriy S. Tchaga
20. Protein Separation Using Immobilized Phospholipid
Chromatography 295
Tzong-Hsien Lee and Marie-Isabel Aguilar
21. Analysis of Proteins in Solution Using Affinity
Capillary Electrophoresis 303
Niels H. H. Heegaard, Christian Schou, and Jesper Ostergaard
Index 339
|
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| dewey-tens | 570 - Biology 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV023307889 |
| illustrated | Illustrated |
| indexdate | 2025-02-03T17:14:18Z |
| institution | BVB |
| isbn | 9781588296597 9781597455824 |
| language | English |
| lccn | 2007930114 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016492220 |
| oclc_num | 191317697 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-12 DE-20 DE-11 |
| owner_facet | DE-19 DE-BY-UBM DE-12 DE-20 DE-11 |
| physical | XII, 343 S. Ill., graph. Darst. 24 cm |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | Affinity chromatography methods and protocols Methods in molecular biology Cromatografia larpcal Affinity chromatography Laboratory manuals Methode (DE-588)4038971-6 gnd Affinitätschromatographie (DE-588)4211538-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4211538-3 |
| title | Affinity chromatography methods and protocols |
| title_auth | Affinity chromatography methods and protocols |
| title_exact_search | Affinity chromatography methods and protocols |
| title_full | Affinity chromatography methods and protocols ed. by Michael Zachariou |
| title_fullStr | Affinity chromatography methods and protocols ed. by Michael Zachariou |
| title_full_unstemmed | Affinity chromatography methods and protocols ed. by Michael Zachariou |
| title_short | Affinity chromatography |
| title_sort | affinity chromatography methods and protocols |
| title_sub | methods and protocols |
| topic | Cromatografia larpcal Affinity chromatography Laboratory manuals Methode (DE-588)4038971-6 gnd Affinitätschromatographie (DE-588)4211538-3 gnd |
| topic_facet | Cromatografia Affinity chromatography Laboratory manuals Methode Affinitätschromatographie |
| url | http://www.loc.gov/catdir/enhancements/fy0826/2007930114-d.html http://www.loc.gov/catdir/enhancements/fy0826/2007930114-t.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016492220&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT zacharioumichael affinitychromatographymethodsandprotocols |