Mesenchymal stem cells methods and protocols
Gespeichert in:
Format: | Buch |
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Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2008
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Schriftenreihe: | Methods in molecular biology
449 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
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Beschreibung: | Includes bibliographical references and index Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.] -- Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger -- A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino -- Adipose-derived stem cells / John K. Fraser ... [et al.] -- Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally -- Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe -- Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe -- Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi -- Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prochop -- Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney |
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Beschreibung: | XVI, 192 S. Ill., graph. Darst. 25 cm |