Cell mechanics
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Schriftenreihe: | Methods in cell biology
83 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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| 007 | t| | ||
| 008 | 071011s2007 xx ad|| |||| 00||| eng d | ||
| 020 | |a 9780123705006 |9 978-0-12-370500-6 | ||
| 035 | |a (OCoLC)157028004 | ||
| 035 | |a (DE-599)BVBBV022876608 | ||
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| 245 | 1 | 0 | |a Cell mechanics |c ed. by Yu-Li Wang |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XX, 608 S., [13] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 83 | |
| 650 | 7 | |a Cellen (biologie) |2 gtt | |
| 650 | 4 | |a Cellules - Propriétés mécaniques | |
| 650 | 4 | |a Hücreler - Mekanik özellikler | |
| 650 | 7 | |a Onderzoeksmethoden |2 gtt | |
| 650 | 4 | |a Biomechanics | |
| 650 | 4 | |a Cell Physiological Phenomena | |
| 650 | 4 | |a Cells |x Mechanical properties | |
| 650 | 4 | |a Cellular Structures | |
| 650 | 4 | |a Mechanotransduction, Cellular | |
| 650 | 0 | 7 | |a Zelle |0 (DE-588)4067537-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biomechanik |0 (DE-588)4006880-8 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Biomechanik |0 (DE-588)4006880-8 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Wang, Yu-Li |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in cell biology |v 83 |w (DE-604)BV002534878 |9 83 | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-016081643 | |
Datensatz im Suchindex
| DE-BY-UBM_katkey | 3693728 |
|---|---|
| DE-BY-UBM_media_number | 99995011105 |
| _version_ | 1823053826626682880 |
| adam_text | CONTENTS
Contributors
Preface
PART I Basic Concept and Preparation Culture Substrates
for Cell Mechanical Studies
1.
Basic Rheology for Biologists
Paul A.Janmey, Penelope C. Georges, and S0ren Hvidt
I. Introduction and Rationale
4
II. Rheological Concepts
6
III. Rheological Instrumentation
11
IV. Experimental Design
13
V. Sample Preparation
20
VI. Special Considerations for Biological Samples
22
VII.
Conclusions
24
References
26
2.
Polyacrylamide
Hydrogels
for Cell Mechanics: Steps Toward Optimization
and Alternative Uses
Casey E.
Randow,
Penelope C. Georges, Paul A.Janmey, and Karen A.
Beningo
I. Introduction
30
II. Principle of the Polyacrylamide Hydrogel
31
III. Conjugation of Proteins to Polyacrylamide
33
IV. Optimizing the Placement of Beads for Traction Force Microscopy
40
V. Manipulation of Gel Geometry
41
VI. Concluding Remarks
. 44
References
45
3.
Microscopic Methods for Measuring the Ehsticity of Gel Substrates for Cell Culture:
Microspheres, Microindenters, and Atomic Force Microscopy
Margo
T.
Frey,
Adam
Engler,
Dennis
E.
Bischer,
Juliet
Lee, and Yu-li
Wang
I. introduction
48
II. Probing with Microspheres Under Gravitational Forces
49
Contents
III. Atomic
Force Microscopy
50
IV. Probing with Spherically Tipped Glass Microindenters
53
V. Conclusions
64
References
64
4.
Surface Patterning
Irene Y. Tsai,
Alfred J.
Crosby, and Thomas P. Russell
I. Introduction
68
II. Patterning with Electrodynamic Instabilities
69
III. Lithography Without a Clean Room
73
IV. Patterning at the Micro- and Nanoscale with Polymer Mixtures and
Block Copolymers
80
V. Summary
84
References
85
5.
Molecular Engineering of Cellular Environments: Cell Adhesion to
Nano-Digital
Surfaces
Joachim P.
Spatz
and
Benjamin Geiger
I. Introduction: Sensing Cellular Environments
90
II.
Nano-Digital
Chemical Surfaces for Regulating
Transmembrane-Receptor Clustering
95
III. Outlook for the Future
108
References
109
PART II Subcellular Mechanical Properties and Activities
6.
Probing Cellular Mechanical Responses to Stimuli Using Ballistic
Intracellular Nanorheology
Porntula Panorchan, Jerry S. H. Lee, Brian R. Daniels, Thomas P.
Kole,
Yiider Tseng, and Denis Wirtz
I. Introduction
117
II. Materials and Instrumentation
127
III. Procedures
129
IV. Pearls and Pitfalls
135
V. Concluding Remarks
136
References
137
Contents
vii
7. Multiple-Particle
Tracking and Two-Point Microrheology in Cells
John C. Crocker and Brenton D. Hoffman
I. Introduction
142
II. Principles of Passive Tracer Microrheology
146
III. Multiple-Particle Tracking Algorithms
149
IV. Computing Rheology from Tracer Trajectories
155
V. Error Sources in Multiple-Particle Tracking
161
VI. Instrument Requirements for High-Performance Tracking
168
VII.
Example: Cultured Epithelial Cells
172
VIII.
Conclusions and Future Directions
177
References
177
8.
Imaging Stress Propagation in the Cytoplasm of a Living Cell
Ning
Wang, Shaohua
Ни,
and James P. Butler
I. Introduction
180
II. Detecting External Stress-Induced Displacements in
the Cytoplasm
181
III. Imaging Displacement and Stress Maps in a Live Cell
188
IV. Future Prospects
197
References
198
9.
Probing Intracellular Force Distributions by High-Resolution Live Cell
Imaging and Inverse Dynamics
Linji, Dinah Loerke, Margaret
Gardeł,
and Gaudenz
Danuser
I. Introduction
200
II. Methods
202
III. Summary
227
IV. Appendix
228
References
231
10.
Analysis of Microtubule Curvature
Andrew D. Bicek,
Brkan
Tüzel,
Daniel
M.
Kroll,
and David
J.
Ödde
I. Introduction
238
II. Rationale
240
III. Raw Data Collection
242
IV. Validation Strategy
245
V. Curvature Estimation Methods
256
VI. Results
258
VII.
Discussion
264
VIII.
Conclusions
265
References
266
Contents
11.
Nuclear
Mechanics and Methods
Jan Lammerding,
Kris Noel
Dahl,
Dennis E. Discher, and Roger D.
Kamm
I. Introduction
270
II. Experimental Methods for Probing Nuclear Mechanical Properties
273
III. Discussion and Prospects
288
IV. Outlook
290
References
291
PART III Cellular and Embryonic Mechanical Properties
and Activities
12.
The Use of Gelatin Substrates for Traction Force Microscopy
in Rapidly Moving Cells
Juliet Lee
I. Introduction
298
II. Rationale
299
HI. Methods
300
IV. Applications of the Gelatin Traction
Porce
Assay to Study Mechano-signal
Transduction in Moving Keratocytes
307
V. Other Applications and Future Directions
309
VI. Summary
310
References
310
13.
Microfabricated
Silicone
Elastomeric Post Arrays for Measuring Traction
Forces of Adherent Cells
Nathan J. Sniadecki and Christopher S. Chen
I. Introduction
314
II. Microfabrication of the
Micropost
Arrays
316
III. Characterization of
Micropost
Spring Constant
321
IV. Analysis of Traction Forces Through
Micropost
Deflections
323
V. Experimental Applications of Microposts and Discussion
326
References
327
14.
Cell Adhesion Strengthening: Measurement and Analysis
Kristin E. Michael and Andres
J
.
Garda
I. Introduction
330
II. The Cell Adhesion Process
330
III. Measurement Systems for Adhesion Characterization
331
Contents
IV.
Hydrodynamic
Assay for Quantifying Adhesion Strength
334
V. Quantitative Biochemical Methods for Adhesion Analysis
338
VI. Simple Mathematical Modeling of Adhesion Strengthening Mechanics
341
VIL
Discussion
344
References
344
15.
Studying the Mechanics of Cellular Processes by Atomic Force Microscopy
Manfred Radmacher
I. Introduction
348
II. Instrumentation and Operation Modes
349
III. Operating Modes
353
IV. Investigations of Live Cells
358
V. Outlook
368
References
369
16.
Using Force to Probe Single-Molecule Receptor—Cytoskeletal Anchoring
Beneath the Surface of a Living Cell
Evan Evans and
Koji
Kinoshita
I. Generic Methods and Physical Foundations
374
II. Probing Bonds at Cell Surfaces
383
III. Future Challenge and Opportunity
393
References
395
17.
High-Throughput Rheological Measurements with an Optical Stretcher
Bryan Lincoln,
Falk
Wottawah, Stefan Schinkinger,
Susanne
Ebert, and
Jochen
Guck
I. Introduction
398
II. Rationale
401
III. Methods
402
IV. Additional Notes on Equipment
412
V. Discussion
419
VI. Summary
421
References
421
18.
Measuring Mechanical Properties of Embryos and Embryonic Tissues
Lance Davidson and Ray Keller
I. Introduction
426
II. Applying and Measuring Forces of
10
nN to
10
μΝ
428
III. Nanonewton Force Apparatus: Parts, Function, and Operation
431
IV. Preparation of Tissue Samples
432
Contents
V. Measurement of the Time-Dependent Elasticity of Embryos or
Tissue
Expiants
434
VI. Spring and Dashpot Models of Viscoelasticity Represent More
Complex Structural Sources
435
VII.
Challenges of Working with Embryonic Tissues
435
VIII.
Use of Standard Engineering Terms and Units
437
IX. Future Prospects
437
References
437
PART IV Mechanical Stimuli to Cells
19.
Tools to Study Cell Mechanics and Mechanotransduction
Tanmay P.
Lele,
Julia E.
Sero, Benjamin D.
Matthews, Sanjay Kumar, Shannon Xia,
Martin
Montoya-
Zavala,
Thomas
Polte,
Darryl Overby,
Ning
Wang, and Donald E. Ingber
I. Introduction
444
II. Control of Cell Shape, Cytoskeletal Organization, and Cell Fate Switching
446
III. Probing Cell Mechanics, Cytoskeletal Structure, and Mechanotransduction
454
IV. Discussion and Future Implications
467
References
469
20.
Magnetic Tweezers in Cell Biology
Monica
Tañase,
Nicolas
Biais,
and Michael Sheetz
I. Introduction
474
II. Physics of Magnetic Tweezers
475
III. Magnetic Field Considerations
477
IV. Magnetic Particle Selection
479
V. Basic Solenoid Apparatus
481
VI. Force Calibration
482
VII.
Experimental Procedures
487
References
491
21.
Optical
Neuronal
Guidance
Allen Ehrlicher,
Timo
Betz, Björn Stuhrmann, Michael Gb gler, Daniel Koch,
Kristian
Frame, Yunbi
Lu, and
Josef Käs
I.
Introduction
496
II. Apparatus
501
III.
Experiments 509
IV.
Plausible
Mechanisms of Optical Guidance
513
Contents Xl
V. Summary
516
References
517
22.
Microtissue
Elasticity: Measurements by Atomic Force Microscopy and Its
Influence on Cell Differentiation
Adam
J
. Engler, Florian
Rehfeldt, Shamik Sen, and Dennis E. Discher
I. Introduction
522
II. AFM in Microelasticity Measurements
526
III. Materials Characterization
531
IV. Assessing Mechanical Influences on Cells
541
References
542
23.
Demystifying the Effects of a Three-Dimensional
Microenvironment
in Tissue Morphogenesis
Kandice R. Johnson, Jennifer L. Leight, and Valerie M. Weaver
I. Introduction
548
II. Rationale
550
III. Methods
558
IV. Materials
573
V. Discussion
577
References
580
Index
585
Volumes in Series
601
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV022876608 |
| illustrated | Illustrated |
| indexdate | 2025-02-03T17:14:18Z |
| institution | BVB |
| isbn | 9780123705006 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016081643 |
| oclc_num | 157028004 |
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| owner_facet | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-703 DE-29T |
| physical | XX, 608 S., [13] Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spellingShingle | Cell mechanics Methods in cell biology Cellen (biologie) gtt Cellules - Propriétés mécaniques Hücreler - Mekanik özellikler Onderzoeksmethoden gtt Biomechanics Cell Physiological Phenomena Cells Mechanical properties Cellular Structures Mechanotransduction, Cellular Zelle (DE-588)4067537-3 gnd Biomechanik (DE-588)4006880-8 gnd |
| subject_GND | (DE-588)4067537-3 (DE-588)4006880-8 (DE-588)4143413-4 |
| title | Cell mechanics |
| title_auth | Cell mechanics |
| title_exact_search | Cell mechanics |
| title_full | Cell mechanics ed. by Yu-Li Wang |
| title_fullStr | Cell mechanics ed. by Yu-Li Wang |
| title_full_unstemmed | Cell mechanics ed. by Yu-Li Wang |
| title_short | Cell mechanics |
| title_sort | cell mechanics |
| topic | Cellen (biologie) gtt Cellules - Propriétés mécaniques Hücreler - Mekanik özellikler Onderzoeksmethoden gtt Biomechanics Cell Physiological Phenomena Cells Mechanical properties Cellular Structures Mechanotransduction, Cellular Zelle (DE-588)4067537-3 gnd Biomechanik (DE-588)4006880-8 gnd |
| topic_facet | Cellen (biologie) Cellules - Propriétés mécaniques Hücreler - Mekanik özellikler Onderzoeksmethoden Biomechanics Cell Physiological Phenomena Cells Mechanical properties Cellular Structures Mechanotransduction, Cellular Zelle Biomechanik Aufsatzsammlung |
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