In vitro culture of mycorrhizas
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| Sprache: | English |
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Springer
2005
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| Schriftenreihe: | Soil biology
4 |
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Datensatz im Suchindex
| _version_ | 1804136627969196032 |
|---|---|
| adam_text | STEPHANE DECLERCK
DESIRE-GEORGES STRULLU
J.-ANDRE FORTI
N (EDS.)
IN VITR
O CULTURE
OF MYCORRHIZAS
WITH 84 FIGURES, 13 IN COLOR
4Y SPRINGER
CONTENTS
PART
I STATE OF THE ART
1 IN VITRO CULTURE OF MYCORRHIZAS 3
/
.
ANDRE FORTIN, STEPHANE DECLERCK, DESIRE-GEORGES STRULLU
1 INTRODUCTIO
N 3
2 A TOOL FOR GERMPLASM COLLECTION 4
3 A TOOL FOR SYSTEMATICS AND BIODIVERSITY 5
4 LIFE CYCLE OF
GLOMUS
SPP 6
5 LIFE HISTORY OF GIGASPORACEAE 6
6 EFFECTS OF ENVIRONMENTAL FACTORS
ON HYPHAL GROWTH AN
D BRANCHING 6
7 QUESTIONING THE VALUE OF MONOXENIC CULTURES 7
8 AM FUNGI; HOST AN
D NON-HOST 7
9 CARBON AN
D LIPID METABOLISM 8
10 MONOXENIC CULTURE AN
D PHYSIOLOGY
OF IN VITRO GROWN PLANTS 9
11 NUTRIEN
T DYNAMICS IN AM MONOXENIC CULTURES 9
12 AM FUNGI AN
D RHIZOSPHERE MICRO-ORGANISMS 10
13
CISTUS INCANUS
ROOT ORGANS TO STUDY ECTOMYCORRHIZAL FUNGI
. 10
14 MONOXENIC CULTURE OF EDIBLE ECTOMYCORRHIZAL FUNGI 11
15 THE UNIQUE
GEOSIPHON
SYMBIOSIS 11
16 SHOULD WE CONSIDER ROOT-INHABITING SEBACINACEAE
AS MYCORRHIZAL FUNGI? 12
17 INDUSTRIAL-SCALE MONOXENIC AM FUNGUS PRODUCTIO
N 12
18 PRECISE TECHNIQUES FOR SUCCESSFUL DEVELOPMENT
OF AM MONOXENIC CULTURE 13
19 CONCLUSION 13
REFERENCES 14
X CONTENTS
PART II SYSTEMATICS
2 THE MONOXENIC CULTURE OF ARBUSCULAR MYCORRHIZAL FUNGI
AS A TOOL FOR GERMPLASM COLLECTIONS 17
STEPHANE DEDERCK, SYLVIE SEGUIN, YOLANDE DALPE
1 INTRODUCTIO
N 17
2 HISTORICAL PERSPECTIVE OF AM FUNGI CULTURE COLLECTIONS 17
3 PREREQUISITE T
O INCLUDE AM FUNGI
IN MONOXENIC CULTURE COLLECTIONS 19
4 CULTURE PROPERTIES
:
VIABILITY - IDENTIT
Y - PURITY - STABILITY (VIPS) 20
5 LONG-TERM CONSERVATION 22
6 STRENGTHS AN
D WEAKNESSES OF MONOXENIC CULTURE COLLECTIONS 25
7 CONCLUSION 28
REFERENCES 29
3 THE MONOXENIC CULTURE OF ARBUSCULAR MYCORRHIZAL FUNGI
AS A TOOL FOR SYSTEMATICS AND BIODIVERSITY 31
YOLANDE DALPE, SYLVIE CRANENBROUCK, SYLVIE SE GUIN,
STEPHANE DEDERCK
1
INTRODUCTIO
N 31
2 SYSTEMATICS 32
2.1 SPECIES AN
D STRAIN AVAILABILITY 32
2.2 FUNGAL MYCELIA AN
D SPORES 33
2.3 BIOCHEMICAL STUDIES 36
2.4 MOLECULAR STUDIES 37
3 BIODIVERSITY 39
3.1 TRAPPING OF ISOLATES 39
3.2 MICRO-MORPHOLOGY 40
3.3 FUNCTIONAL DIVERSITY 41
4 CONCLUSION 43
REFERENCES 43
4 LIFE CYCLE OF
GLOMUS
SPECIES IN MONOXENIC CULTURE 49
YOLANDE DALPE, FRANDSCO ADRIANO DE SOUZA, STEPHANE DEDERCK
1 INTRODUCTIO
N 49
2 LIFE CYCLE 49
3 AM FUNGI PROPAGULE GERMINATION STAGE 50
4 PRE-SYMBIOTIC MYCELIUM STAGE 53
5 HOST ROOT CONNECTING STAGE 54
6 SYMBIOTIC STAGE 55
6.1 INTRARADICA
L MYCELIUM 56
CONTENTS XI
6.2 EXTRARADICAL MYCELIUM 58
7 SPORES 61
8 CONCLUSION 65
REFERENCES 65
5
LIFE HISTORY STRATEGIES I
N GIGASPORACEAE:
INSIGHT FROM MONOXENIC CULTURE 73
FRANCISCO ADRIANO DE SOUZA, YOLANDE DALPE, STEPHANE DEDERCK,
IVAN ENRIQUE DE LA PROVIDENCIA, NATHALIE SEJALON-DELMAS
1 INTRODUCTIO
N 73
2 THE FAMILY GIGASPORACEAE AN
D ITS OCCURRENCE 73
3 LIFE CYCLE 74
3.1 PRE-SYMBIOTIC PHASE 74
3.2 SYMBIOTIC VEGETATIVE
AN
D REPRODUCTIVE GROWING PHASES
77
4 GENETIC DIVERSITY AN
D PHENOTYPIC VARIATION 80
4.1 VEGETATIVE COMPATIBILITY TEST (VCT) 81
5 LIFE HISTORY STRATEGY (LHS) OF GIGASPORACEAE,
AS REVEALED USING MONOXENIC CULTURES 82
5.1 CO-EXISTENCE AN
D COMPETITION EXPERIMENTS
UNDER DIXENIC CULTURE 85
5.2 ECOLOGICAL IMPLICATIONS
OF THEGIGASPORACEA
E LIFE HISTORY STRATEGY 85
6 CONCLUSIONS 87
REFERENCES 88
PART III IN VITRO DEVELOPMENT AND PHYSIOLOGY OF GLOMEROMYCETES
6 ENVIRONMENTAL FACTORS
THAT AFFECT PRESYMBIOTIC HYPHAL GROWTH AND BRANCHING
OF ARBUSCULAR MYCORRHIZAL FUNGI 95
GERALD NAGAHASHI, DAVID D. DOUDS JR.
1 INTRODUCTIO
N 95
2 IN VITRO TECHNIQUES 96
2.1 SPORE PRODUCTIO
N AN
D GERMINATION 96
2.2 ROOT ORGAN CULTURES AN
D ROOT EXUDATES 96
2.3 INCUBATION CONDITIONS
WITH CO
2
AN
D EXUDATE TREATMENTS 97
2.4 LIGHT EXPERIMENTS 97
2.5 SYNERGISTIC EFFECTS BETWEEN CHEMICAL COMPOUND
S 98
XII CONTENTS
3 CHEMICAL COMPONENTS OF EXUDATES OR COMPOUND
S
FOUND IN TH
E SOIL ENVIRONMENT
THA
T INFLUENCE PRESYMBIOTIC AM FUNGAL GROWTH 98
4 EFFECTS OF VOLATILE COMPOUNDS
ON PRESYMBIOTIC AM FUNGAL GROWTH 100
4.1 EFFECTS OF CO
2
ON THE HYPHAL GROWTH
OF GERMINATED
GIGASPORA GIGANTEA
SPORES 101
5 THE EFFECT OF LIGHT ON TH
E PRESYMBIOTIC GROWTH
OF AM FUNGI 103
6 CONCLUSIONS 106
REFERENCES 108
7
BREAKING MYTHS ON ARBUSCULAR MYCORRHIZAS IN VITRO BIOLOGY 111
BERT BAGO, CUSTODIA CANO
1 INTRODUCTIO
N IL
L
2 QUESTIONING AM MONOXENIC CULTURES 113
2.1 ARE AM MONOXENIC CULTURES
DEVICES TOO ARTIFICIAL T
O TRUST? 113
2.2 ARE TRANSFORMED ROOT ORGANS A GOOD HOST MATERIAL
T
O STUDY AM FUNGAL BIOLOGY? 116
2.3 THE DOWNFALL OF TWO COLONIZATION MYTHS 118
2.4 ARE BRANCHED ABSORBING STRUCTURES (BAS) COMMONLY
FORMED B
Y ALL GLOMALEAN FUNGI? ARE THEY ARTIFACTS
FORMED ONLY UNDER IN VITRO CONDITIONS? 119
2.5 ARE THERE ANY DIFFERENCES
IN AM FUNGAL DEVELOPMENT
IN MONOXENICS VERSUS SOIL? 124
2.6 ARE AM MONOXENIC LIQUID CULTURES ACCURATE? 126
2.7 THE DANGER OF CONTAMINATION
IN AM MONOXENIC CULTURES 128
2.8 WHAT ELSE HAVE MONOXENIC CULTURES
TO OFFER O
N THE STUDY OF AM FUNGAL BIOLOGY? 129
3 CONCLUSION 133
REFERENCES 133
8 HOST AND NON-HOST IMPACT ON THE PHYSIOLOGY
OF THE AM SYMBIOSIS 139
HORST VIERHEILIG, BERT BAGO
1
INTRODUCTIO
N 139
2 ASYMBIOTIC AM FUNGAL GROWTH 140
2.1 P
H 141
2.2 TEMPERATURE 141
CONTENTS XIII
2.3 CO
2
142
2.4 LIGHT 143
3 PRE-SYMBIOTIC AM FUNGAL GROWTH 143
3.1 PLANT-TO-FUNGUS SIGNALS 144
3.2 AM FUNGUS-TO-PLANT SIGNALS 150
4 SYMBIOTIC AM FUNGAL GROWTH 151
5 CONCLUSION 153
REFERENCES 153
9 CARBON METABOLISM, LIPID COMPOSITION AND METABOLISM
IN ARBUSCULAR MYCORRHIZAL FUNGI 159
ANNE GRANDMOUGIN-FERJANI, JOEL FONTAINE, ROGER DURAND
1 INTRODUCTIO
N 159
2 SUGAR METABOLISM IN AMF 160
3 MONOXENIC CULTIVATION TECHNIQUES
AS A TOOL FOR TH
E ESTABLISHMENT
OF TH
E LIPID COMPOSITION OF AM FUNGI 162
3.1 LIPID CLASSES OF
GLOMUS INTRARADICES
163
3.2 TOTAL FA PROFILES OF G.
INTRARADICES
163
3.3 FA PROFILES OF
G. INTRARADICES
AFTER SEPARATION OF DIFFERENT LIPID FRACTIONS 165
3.4 PHOSPHOLIPID COMPOSITION 167
3.5 STEROL COMPOSITION 167
4 MONOXENIC CULTURE TECHNIQUES
AS A TOOL FOR THE ESTABLISHMENT
OF LIPID INDICATORS OF THE PRESENCE OF AM FUNGI IN ROOTS 170
5 LIPID METABOLISM 170
5.1
14
C LABELLING 171
5.2
13
C LABELLING 173
5.3 LIPID SYNTHESIS IN ARBUSCULAR MYCORRHIZAE:
TH
E CONTROVERSY 174
6 CONCLUSIONS 175
REFERENCES 176
10 MONOXENIC CULTURE AS A TOOL TO STUDY THE EFFECT
OF THE ARBUSCULAR MYCORRHIZAL SYMBIOSIS
ON THE PHYSIOLOGY OF MICROPROPAGATED PLANTLETS
IN VITRO AND EX VITRO 181
YVES DESJARDINS, CINTA HERNDNDEZ-SEBASTIA, YVES PICHE
1 INTRODUCTIO
N 181
2 THE TRIPARTITE CULTURE SYSTEM 182
XIV CONTENTS
3 THE TRIPARTITE CULTURE SYSTEM T
O STUDY TH
E ADAPTATION
OF PLANTS TO WATER STRESS 187
3.1 INTRARADICA
L PHASE OF AM FUNGI 190
3.2 EXTRARADICAL PHASE OF AM FUNGI 191
4 THE TRIPARTITE CULTURE SYSTEM
T
O STUDY SINK-SOURCE RELATIONSHIPS 194
5 CONCLUSIONS 196
REFERENCES 197
11 UPTAKE, ASSIMILATION AND TRANSLOCATION OF MINERAL ELEMENTS
IN MONOXENIC CULTIVATION SYSTEMS 201
GERVAIS RUFYIKIRI, NATHALIE KRUYTS, STEPHANE DEDERCK,
YVES THIRY, BRUNO DELVAUX, HERVE DUPRE DE BOULOIS, ERIK JONER
1 INTRODUCTIO
N 201
2 NUTRIEN
T UPTAKE AN
D TRANSLOCATION BY AM FUNGI 203
2.1 PHOSPHORU
S 203
2.2 NITROGEN 204
3 NON-ESSENTIAL ELEMENT UPTAKE AN
D TRANSLOCATION
B
Y AM FUNGI 205
3.1 URANIU
M 205
3.2 CAESIUM 209
4 CONCLUSION 211
REFERENCES 212
12 INTERACTION OF ARBUSCULAR MYCORRHIZAL FUNGI
WITH SOIL-BORNE PATHOGENS
AND NON-PATHOGENIC RHIZOSPHERE MICRO-ORGANISMS 217
MARC STARNAUD, ANNEMIE ELSEN
1 INTRODUCTIO
N 217
2 INTERACTIO
N BETWEEN AM FUNGI AN
D SOIL BACTERIA 218
3 INTERACTIO
N BETWEEN AM FUNGI AN
D OTHER FUNGI 221
4 INTERACTION BETWEEN AM FUNGI AN
D NEMATODES 225
5 CONCLUSION 226
REFERENCES 227
PART IV ROOT ORGAN CULTURE OF ECTOMYCORRHIZAL FUNGI
13
CISTUS
ICANUS
ROOT ORGAN CULTURES:
A VALUABLE TOOL FOR STUDYING MYCORRHIZAL ASSOCIATIONS
235
ANDREW P. COUGHLAN, YVES RICHI
1 INTRODUCTIO
N 235
CONTENTS XV
2 EVOLUTION OF MONOXENIC TECHNIQUES
FOR INVESTIGATING ECM ASSOCIATIONS 237
2.1 WHOLE-PLANT TECHNIQUES 237
2.2 NON-TRANSFORMED ROOT ORGANS
AN
D ROOT HYPOCOTYL ORGANS 238
2.3 RI T-DNA TRANSFORMED ROOT ORGANS 239
2.4 RI T-DNA TRANSFORMED ROOT ORGANS
OF CISTUS INCANUS
240
3 PRODUCTIO
N AN
D MAINTENANCE
OF TRANSFORMED
CISTUS INCANUS
ROOT ORGANS 240
4 USE OF
CISTUS INCANUS
ROOT ORGANS
FOR TH
E STUDY OF MYCORRHIZAL ASSOCIATIONS 241
4.1 EFFECT OF
CISTUS INCANUS
ROOT ORGANS
ON ECM FUNGAL GROWTH 241
4.2 DIFFERENTIATION OF ECM FUNGAL MYCELIUM
IN THE PRESENCE
OF CISTUS INCANUS
ROOT ORGANS 243
4.3 FORMATION OF ECM ON
CISTUS INCANUS
ROOT ORGANS 243
4.4 USE OF
CISTUS INCANUS
ROOT ORGANS
FOR WORK WIT
H AM FUNGI 245
5 PRACTICAL APPLICATIONS OF
CISTUS INCANUS
ROOT ORGANS 245
5.1 MAINTAINING FUNGAL ISOLATES 245
5.2 INOCULUM PRODUCTIO
N 246
6 CONCLUSION 247
REFERENCES 249
14 CULTIVATION OF EDIBLE ECTOMYCORRHIZAL FUNGI
BY IN VITRO MYCORRHIZAL SYNTHESIS
253
GIOVANNA MARIA GIOMARO, DAVIDE SISTI, ALESSANDRA ZAMBONELLI
1 INTRODUCTIO
N 253
2 METHODS FOR SYNTHESIZING ECTOMYCORRHIZAS 254
2.1 SPORAL INOCULU
M 255
2.2 MOTHER-PLANT TECHNIQUE 255
2.3 MYCELIAL INOCULATION 257
2.4 CULTURE MEDIA 259
2.5 MYCORRHIZAL SYNTHESIS IN VITRO 260
3 IN VITRO RESULTS TO DATE 261
4 CONCLUSION 263
REFERENCES 263
XVI CONTENTS
PART V ROOT ORGAN CULTURE OF OTHER FUNGAL SYMBIOSES
15
GEOSIPHON PYRIFORMIS
A GLOMEROMYCOTAN SOIL FUNGUS
FORMING ENDOSYMBIOSIS WITH CYANOBACTERIA 271
ARTHUR SCHUFILER, ELKE WOLF
1 INTRODUCTIO
N 271
2 DEVELOPMENT AN
D STRUCTURE OF THE SYMBIOTIC CONSORTIU
M ...
. 271
2.1 STRUCTURE OF TH
E MATURE SYMBIOSIS 271
2.2 SPECIFICITY OF PARTNE
R RECOGNITION AN
D DEVELOPMENT
OF THE SYMBIOSIS 274
3 ECOLOGY AND DISTRIBUTIO
N OF TH
E
GEOSIPHON
SYMBIOSIS 274
3.1 ECOLOGY OF THE
GEOSIPHON
SYMBIOSIS 274
3.2 REPORTS AN
D DISTRIBUTIO
N OF THE
GEOSIPHON
SYMBIOSIS
. 275
3.3 AN ECOLOGICAL NETWORK
BETWEEN FUNGI, CYANOBACTERIA AN
D PLANTS? 276
4 CULTURE SYSTEMS FOR TH
E
GEOSIPHON
SYMBIOSIS 277
4.1 LABORATORY CULTURE SYSTEMS 277
4.2 CULTURE SYSTEMS FOR MICROSCOPIC INVESTIGATIONS 278
4.3 SYNCHRONIZATION OF THE
NOSTOC
LIFE CYCLE 279
5 METABOLIC ASPECTS OF THE SYMBIOSIS 280
5.1 C AN
D N METABOLISM 280
5.2 UPTAKE AN
D CONTENT OF INORGANIC NUTRIENT
S 281
5.3 HEAVY METAL UPTAKE AN
D RESISTANCE 282
6 PHYLOGENY AN
D TAXONOMY OF
GEOSIPHON
AN
D AM FUNGI 282
6.1
GEOSIPHON
IS AN AM FUNGUS
282
6.2 THE ORIGIN AN
D EVOLUTION
OF AM FUNGI AN
D THE AM SYMBIOSIS 284
7 THE
GEOSIPHON
SYMBIOSIS - A MODEL SYSTEM FOR AM? 285
7.1 PARTNE
R RECOGNITION AN
D SYMBIOSIS ESTABLISHMENT 285
7.2 BACTERIAL ENDOSYMBIONTS (BLOS) 285
7.3 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED
FUNGAL GENES 286
8 CONCLUSIONS 287
REFERENCES 287
16 SEBACINACEAE: CULTURABLE MYCORRHIZA-LIKE ENDOSYMBIOTIC FUNGI
AND THEIR INTERACTION
WITH NON-TRANSFORMED AND TRANSFORMED ROOTS 291
RAM PRASAD, HUONG GIANGPHAM, RINA KUMARI, ANJANA SINGH,
VIKAS YADAV, MINU SACHDEV, AMAR PRAKASH GARG, TATJANA PESKAN,
SOLVEIGHEHL, IRENA SHERAMETI, RALF OELMULLER, AJIT VARMA
1 INTRODUCTIO
N 291
CONTENTS XVII
2 SEBACINACEOUS FUNGI 292
3 HOST RANGE AN
D GROWTH PROMOTIO
N EFFECT
OF SEBACINACEOUS FUNGI 294
4 ECO-FUNCTIONAL IDENTITY 294
5 AXENIC CULTIVATION 298
6 MONOXENIC CULTURE 300
7 INTERACTIO
N WITH TRANSFORMED ROOTS 300
8 INTERACTIO
N WITH NON-TRANSFORMED ROOTS 303
9 CONCLUSION 309
REFERENCES 310
PART VI BIOTECHNOLOGY
17 LARGE-SCALE INOCULUM PRODUCTION OF ARBUSCULAR MYCORRHIZAL FUNGI
ON ROOT ORGANS AND INOCULATION STRATEGIES 315
ALOK ADHOLEYA, PRAGATI TIWARI, REENA SINGH
1 INTRODUCTIO
N 315
2 MONOXENIC-BASED AM INOCULUM PRODUCTIO
N 316
3 FORMULATIONS 318
4 INOCULATION STRATEGIES AN
D APPLICATION TECHNOLOGY 319
5 APPLICATION TECHNOLOGY 320
5.1 BROADCASTING 321
5.2 IN-FURROW APPLICATION 322
5.3 SEED DRESSING 322
5.4 ROOT DIPPING 322
5.5 SEEDLING INOCULATION 323
6 FIELD EVALUATION 323
6.1 FACTORS AFFECTING TH
E FIELD INOCULATION 326
7 RESPONSIBILITY OF TH
E SCIENTIFIC COMMUNIT
Y
TOWARDS TECHNOLOGY DEVELOPMENT 327
7.1 QUALITY CONTROL AND BENCHMARKING 328
8 SCOPES AN
D APPLICATIONS OF MONOXENICALLY BASED
AM FUNGAL TECHNOLOGY 329
8.1 CONSORTIU
M DEVELOPMENT OF AM FUNGI 329
8.2 HOST-BASED ENHANCED PRODUCTIVITY
IN AM FUNGUS PRODUCTIO
N 330
8.3 MYCORRHIZAE AN
D COUPLING BENEFICIAL ORGANISMS:
COMPLETE PACKAGE FOR ORGANIC SYSTEMS 330
9 POTENTIAL TOOL FOR ORGANIC FARMING 332
10 CONCLUSIONS 332
REFERENCES 333
XVIII CONTENTS
PART VII METHODOLOGY
18 METHODOLOGIES FOR IN VITRO CULTIVATION
OF ARBUSCULAR MYCORRHIZAL FUNGI WITH ROOT ORGANS 341
SYLVIE CRANENBROUCK, LIESBETH VOETS, CELINE BIVORT,
LAURENT RENARD, DESIRE-GEORGES STRULLU, STEPHANE DEDERCK
1 INTRODUCTIO
N 341
2 PROCESS DESCRIPTION 342
3 SELECTION OF TH
E CULTURE SYSTEM 342
4 CULTURE MEDIA PREPARATIO
N 347
4.1 MATERIAL 349
4.2 STOCK SOLUTIONS FOR MSR MEDIUM 349
4.3 MEDIUM PREPARATIO
N 350
5 HOST ROOT 351
5.1 CHOICE OF HOST ROOT 351
5.2 HOST ROOT TRANSFORMATION 353
5.3 HOST ROOT CULTIVATION 354
6 AM FUNGAL PROPAGULES: SELECTION AN
D DISINFECTION 356
6.1 SELECTION OF PROPAGULE 356
6.2 DISINFECTION PROCESS 357
7 MONOXENIC CULTURE ESTABLISHMENT 365
7.1 GERMINATION OF DISINFECTED PROPAGULES 365
7.2 MATERIAL 366
7.3 ASSOCIATION METHODS 366
8 CONTINUOUS CULTURE 368
8.1 ASSOCIATION ESTABLISHMENT 368
8.2 CONTINUOUS CULTURE 368
8.3 MATERIAL 369
8.4 SOLUTIONS 369
8.5 FIRST METHOD: PROPAGULE RE-ASSOCIATION 370
8.6 SECOND METHOD: MYCORRHIZED-APEX TRANSFER 371
9 CONCLUSION 372
REFERENCES 372
SUBJECT INDEX 377
|
| adam_txt |
STEPHANE DECLERCK
DESIRE-GEORGES STRULLU
J.-ANDRE FORTI
N (EDS.)
IN VITR
O CULTURE
OF MYCORRHIZAS
WITH 84 FIGURES, 13 IN COLOR
4Y SPRINGER
CONTENTS
PART
I STATE OF THE ART
1 IN VITRO CULTURE OF MYCORRHIZAS 3
/
.
ANDRE FORTIN, STEPHANE DECLERCK, DESIRE-GEORGES STRULLU
1 INTRODUCTIO
N 3
2 A TOOL FOR GERMPLASM COLLECTION 4
3 A TOOL FOR SYSTEMATICS AND BIODIVERSITY 5
4 LIFE CYCLE OF
GLOMUS
SPP 6
5 LIFE HISTORY OF GIGASPORACEAE 6
6 EFFECTS OF ENVIRONMENTAL FACTORS
ON HYPHAL GROWTH AN
D BRANCHING 6
7 QUESTIONING THE VALUE OF MONOXENIC CULTURES 7
8 AM FUNGI; HOST AN
D NON-HOST 7
9 CARBON AN
D LIPID METABOLISM 8
10 MONOXENIC CULTURE AN
D PHYSIOLOGY
OF IN VITRO GROWN PLANTS 9
11 NUTRIEN
T DYNAMICS IN AM MONOXENIC CULTURES 9
12 AM FUNGI AN
D RHIZOSPHERE MICRO-ORGANISMS 10
13
CISTUS INCANUS
ROOT ORGANS TO STUDY ECTOMYCORRHIZAL FUNGI
. 10
14 MONOXENIC CULTURE OF EDIBLE ECTOMYCORRHIZAL FUNGI 11
15 THE UNIQUE
GEOSIPHON
SYMBIOSIS 11
16 SHOULD WE CONSIDER ROOT-INHABITING SEBACINACEAE
AS MYCORRHIZAL FUNGI? 12
17 INDUSTRIAL-SCALE MONOXENIC AM FUNGUS PRODUCTIO
N 12
18 PRECISE TECHNIQUES FOR SUCCESSFUL DEVELOPMENT
OF AM MONOXENIC CULTURE 13
19 CONCLUSION 13
REFERENCES 14
X CONTENTS
PART II SYSTEMATICS
2 THE MONOXENIC CULTURE OF ARBUSCULAR MYCORRHIZAL FUNGI
AS A TOOL FOR GERMPLASM COLLECTIONS 17
STEPHANE DEDERCK, SYLVIE SEGUIN, YOLANDE DALPE
1 INTRODUCTIO
N 17
2 HISTORICAL PERSPECTIVE OF AM FUNGI CULTURE COLLECTIONS 17
3 PREREQUISITE T
O INCLUDE AM FUNGI
IN MONOXENIC CULTURE COLLECTIONS 19
4 CULTURE PROPERTIES
:
VIABILITY - IDENTIT
Y - PURITY - STABILITY (VIPS) 20
5 LONG-TERM CONSERVATION 22
6 STRENGTHS AN
D WEAKNESSES OF MONOXENIC CULTURE COLLECTIONS 25
7 CONCLUSION 28
REFERENCES 29
3 THE MONOXENIC CULTURE OF ARBUSCULAR MYCORRHIZAL FUNGI
AS A TOOL FOR SYSTEMATICS AND BIODIVERSITY 31
YOLANDE DALPE, SYLVIE CRANENBROUCK, SYLVIE SE"GUIN,
STEPHANE DEDERCK
1
INTRODUCTIO
N 31
2 SYSTEMATICS 32
2.1 SPECIES AN
D STRAIN AVAILABILITY 32
2.2 FUNGAL MYCELIA AN
D SPORES 33
2.3 BIOCHEMICAL STUDIES 36
2.4 MOLECULAR STUDIES 37
3 BIODIVERSITY 39
3.1 TRAPPING OF ISOLATES 39
3.2 MICRO-MORPHOLOGY 40
3.3 FUNCTIONAL DIVERSITY 41
4 CONCLUSION 43
REFERENCES 43
4 LIFE CYCLE OF
GLOMUS
SPECIES IN MONOXENIC CULTURE 49
YOLANDE DALPE, FRANDSCO ADRIANO DE SOUZA, STEPHANE DEDERCK
1 INTRODUCTIO
N 49
2 LIFE CYCLE 49
3 AM FUNGI PROPAGULE GERMINATION STAGE 50
4 PRE-SYMBIOTIC MYCELIUM STAGE 53
5 HOST ROOT CONNECTING STAGE 54
6 SYMBIOTIC STAGE 55
6.1 INTRARADICA
L MYCELIUM 56
CONTENTS XI
6.2 EXTRARADICAL MYCELIUM 58
7 SPORES 61
8 CONCLUSION 65
REFERENCES 65
5
LIFE HISTORY STRATEGIES I
N GIGASPORACEAE:
INSIGHT FROM MONOXENIC CULTURE 73
FRANCISCO ADRIANO DE SOUZA, YOLANDE DALPE, STEPHANE DEDERCK,
IVAN ENRIQUE DE LA PROVIDENCIA, NATHALIE SEJALON-DELMAS
1 INTRODUCTIO
N 73
2 THE FAMILY GIGASPORACEAE AN
D ITS OCCURRENCE 73
3 LIFE CYCLE 74
3.1 PRE-SYMBIOTIC PHASE 74
3.2 SYMBIOTIC VEGETATIVE
AN
D REPRODUCTIVE GROWING PHASES
77
4 GENETIC DIVERSITY AN
D PHENOTYPIC VARIATION 80
4.1 VEGETATIVE COMPATIBILITY TEST (VCT) 81
5 LIFE HISTORY STRATEGY (LHS) OF GIGASPORACEAE,
AS REVEALED USING MONOXENIC CULTURES 82
5.1 CO-EXISTENCE AN
D COMPETITION EXPERIMENTS
UNDER DIXENIC CULTURE 85
5.2 ECOLOGICAL IMPLICATIONS
OF THEGIGASPORACEA
E LIFE HISTORY STRATEGY 85
6 CONCLUSIONS 87
REFERENCES 88
PART III IN VITRO DEVELOPMENT AND PHYSIOLOGY OF GLOMEROMYCETES
6 ENVIRONMENTAL FACTORS
THAT AFFECT PRESYMBIOTIC HYPHAL GROWTH AND BRANCHING
OF ARBUSCULAR MYCORRHIZAL FUNGI 95
GERALD NAGAHASHI, DAVID D. DOUDS JR.
1 INTRODUCTIO
N 95
2 IN VITRO TECHNIQUES 96
2.1 SPORE PRODUCTIO
N AN
D GERMINATION 96
2.2 ROOT ORGAN CULTURES AN
D ROOT EXUDATES 96
2.3 INCUBATION CONDITIONS
WITH CO
2
AN
D EXUDATE TREATMENTS 97
2.4 LIGHT EXPERIMENTS 97
2.5 SYNERGISTIC EFFECTS BETWEEN CHEMICAL COMPOUND
S 98
XII CONTENTS
3 CHEMICAL COMPONENTS OF EXUDATES OR COMPOUND
S
FOUND IN TH
E SOIL ENVIRONMENT
THA
T INFLUENCE PRESYMBIOTIC AM FUNGAL GROWTH 98
4 EFFECTS OF VOLATILE COMPOUNDS
ON PRESYMBIOTIC AM FUNGAL GROWTH 100
4.1 EFFECTS OF CO
2
ON THE HYPHAL GROWTH
OF GERMINATED
GIGASPORA GIGANTEA
SPORES 101
5 THE EFFECT OF LIGHT ON TH
E PRESYMBIOTIC GROWTH
OF AM FUNGI 103
6 CONCLUSIONS 106
REFERENCES 108
7
BREAKING MYTHS ON ARBUSCULAR MYCORRHIZAS IN VITRO BIOLOGY 111
BERT BAGO, CUSTODIA CANO
1 INTRODUCTIO
N IL
L
2 QUESTIONING AM MONOXENIC CULTURES 113
2.1 ARE AM MONOXENIC CULTURES
DEVICES TOO ARTIFICIAL T
O TRUST? 113
2.2 ARE TRANSFORMED ROOT ORGANS A GOOD HOST MATERIAL
T
O STUDY AM FUNGAL BIOLOGY? 116
2.3 THE DOWNFALL OF TWO COLONIZATION MYTHS 118
2.4 ARE BRANCHED ABSORBING STRUCTURES (BAS) COMMONLY
FORMED B
Y ALL GLOMALEAN FUNGI? ARE THEY ARTIFACTS
FORMED ONLY UNDER IN VITRO CONDITIONS? 119
2.5 ARE THERE ANY DIFFERENCES
IN AM FUNGAL DEVELOPMENT
IN MONOXENICS VERSUS SOIL? 124
2.6 ARE AM MONOXENIC LIQUID CULTURES ACCURATE? 126
2.7 THE DANGER OF CONTAMINATION
IN AM MONOXENIC CULTURES 128
2.8 WHAT ELSE HAVE MONOXENIC CULTURES
TO OFFER O
N THE STUDY OF AM FUNGAL BIOLOGY? 129
3 CONCLUSION 133
REFERENCES 133
8 HOST AND NON-HOST IMPACT ON THE PHYSIOLOGY
OF THE AM SYMBIOSIS 139
HORST VIERHEILIG, BERT BAGO
1
INTRODUCTIO
N 139
2 ASYMBIOTIC AM FUNGAL GROWTH 140
2.1 P
H 141
2.2 TEMPERATURE 141
CONTENTS XIII
2.3 CO
2
142
2.4 LIGHT 143
3 PRE-SYMBIOTIC AM FUNGAL GROWTH 143
3.1 PLANT-TO-FUNGUS SIGNALS 144
3.2 AM FUNGUS-TO-PLANT SIGNALS 150
4 SYMBIOTIC AM FUNGAL GROWTH 151
5 CONCLUSION 153
REFERENCES 153
9 CARBON METABOLISM, LIPID COMPOSITION AND METABOLISM
IN ARBUSCULAR MYCORRHIZAL FUNGI 159
ANNE GRANDMOUGIN-FERJANI, JOEL FONTAINE, ROGER DURAND
1 INTRODUCTIO
N 159
2 SUGAR METABOLISM IN AMF 160
3 MONOXENIC CULTIVATION TECHNIQUES
AS A TOOL FOR TH
E ESTABLISHMENT
OF TH
E LIPID COMPOSITION OF AM FUNGI 162
3.1 LIPID CLASSES OF
GLOMUS INTRARADICES
163
3.2 TOTAL FA PROFILES OF G.
INTRARADICES
163
3.3 FA PROFILES OF
G. INTRARADICES
AFTER SEPARATION OF DIFFERENT LIPID FRACTIONS 165
3.4 PHOSPHOLIPID COMPOSITION 167
3.5 STEROL COMPOSITION 167
4 MONOXENIC CULTURE TECHNIQUES
AS A TOOL FOR THE ESTABLISHMENT
OF LIPID INDICATORS OF THE PRESENCE OF AM FUNGI IN ROOTS 170
5 LIPID METABOLISM 170
5.1
14
C LABELLING 171
5.2
13
C LABELLING 173
5.3 LIPID SYNTHESIS IN ARBUSCULAR MYCORRHIZAE:
TH
E CONTROVERSY 174
6 CONCLUSIONS 175
REFERENCES 176
10 MONOXENIC CULTURE AS A TOOL TO STUDY THE EFFECT
OF THE ARBUSCULAR MYCORRHIZAL SYMBIOSIS
ON THE PHYSIOLOGY OF MICROPROPAGATED PLANTLETS
IN VITRO AND EX VITRO 181
YVES DESJARDINS, CINTA HERNDNDEZ-SEBASTIA, YVES PICHE
1 INTRODUCTIO
N 181
2 THE TRIPARTITE CULTURE SYSTEM 182
XIV CONTENTS
3 THE TRIPARTITE CULTURE SYSTEM T
O STUDY TH
E ADAPTATION
OF PLANTS TO WATER STRESS 187
3.1 INTRARADICA
L PHASE OF AM FUNGI 190
3.2 EXTRARADICAL PHASE OF AM FUNGI 191
4 THE TRIPARTITE CULTURE SYSTEM
T
O STUDY SINK-SOURCE RELATIONSHIPS 194
5 CONCLUSIONS 196
REFERENCES 197
11 UPTAKE, ASSIMILATION AND TRANSLOCATION OF MINERAL ELEMENTS
IN MONOXENIC CULTIVATION SYSTEMS 201
GERVAIS RUFYIKIRI, NATHALIE KRUYTS, STEPHANE DEDERCK,
YVES THIRY, BRUNO DELVAUX, HERVE" DUPRE DE BOULOIS, ERIK JONER
1 INTRODUCTIO
N 201
2 NUTRIEN
T UPTAKE AN
D TRANSLOCATION BY AM FUNGI 203
2.1 PHOSPHORU
S 203
2.2 NITROGEN 204
3 NON-ESSENTIAL ELEMENT UPTAKE AN
D TRANSLOCATION
B
Y AM FUNGI 205
3.1 URANIU
M 205
3.2 CAESIUM 209
4 CONCLUSION 211
REFERENCES 212
12 INTERACTION OF ARBUSCULAR MYCORRHIZAL FUNGI
WITH SOIL-BORNE PATHOGENS
AND NON-PATHOGENIC RHIZOSPHERE MICRO-ORGANISMS 217
MARC STARNAUD, ANNEMIE ELSEN
1 INTRODUCTIO
N 217
2 INTERACTIO
N BETWEEN AM FUNGI AN
D SOIL BACTERIA 218
3 INTERACTIO
N BETWEEN AM FUNGI AN
D OTHER FUNGI 221
4 INTERACTION BETWEEN AM FUNGI AN
D NEMATODES 225
5 CONCLUSION 226
REFERENCES 227
PART IV ROOT ORGAN CULTURE OF ECTOMYCORRHIZAL FUNGI
13
CISTUS
ICANUS
ROOT ORGAN CULTURES:
A VALUABLE TOOL FOR STUDYING MYCORRHIZAL ASSOCIATIONS
235
ANDREW P. COUGHLAN, YVES RICHI
1 INTRODUCTIO
N 235
CONTENTS XV
2 EVOLUTION OF MONOXENIC TECHNIQUES
FOR INVESTIGATING ECM ASSOCIATIONS 237
2.1 WHOLE-PLANT TECHNIQUES 237
2.2 NON-TRANSFORMED ROOT ORGANS
AN
D ROOT HYPOCOTYL ORGANS 238
2.3 RI T-DNA TRANSFORMED ROOT ORGANS 239
2.4 RI T-DNA TRANSFORMED ROOT ORGANS
OF CISTUS INCANUS
240
3 PRODUCTIO
N AN
D MAINTENANCE
OF TRANSFORMED
CISTUS INCANUS
ROOT ORGANS 240
4 USE OF
CISTUS INCANUS
ROOT ORGANS
FOR TH
E STUDY OF MYCORRHIZAL ASSOCIATIONS 241
4.1 EFFECT OF
CISTUS INCANUS
ROOT ORGANS
ON ECM FUNGAL GROWTH 241
4.2 DIFFERENTIATION OF ECM FUNGAL MYCELIUM
IN THE PRESENCE
OF CISTUS INCANUS
ROOT ORGANS 243
4.3 FORMATION OF ECM ON
CISTUS INCANUS
ROOT ORGANS 243
4.4 USE OF
CISTUS INCANUS
ROOT ORGANS
FOR WORK WIT
H AM FUNGI 245
5 PRACTICAL APPLICATIONS OF
CISTUS INCANUS
ROOT ORGANS 245
5.1 MAINTAINING FUNGAL ISOLATES 245
5.2 INOCULUM PRODUCTIO
N 246
6 CONCLUSION 247
REFERENCES 249
14 CULTIVATION OF EDIBLE ECTOMYCORRHIZAL FUNGI
BY IN VITRO MYCORRHIZAL SYNTHESIS
253
GIOVANNA MARIA GIOMARO, DAVIDE SISTI, ALESSANDRA ZAMBONELLI
1 INTRODUCTIO
N 253
2 METHODS FOR SYNTHESIZING ECTOMYCORRHIZAS 254
2.1 SPORAL INOCULU
M 255
2.2 MOTHER-PLANT TECHNIQUE 255
2.3 MYCELIAL INOCULATION 257
2.4 CULTURE MEDIA 259
2.5 MYCORRHIZAL SYNTHESIS IN VITRO 260
3 IN VITRO RESULTS TO DATE 261
4 CONCLUSION 263
REFERENCES 263
XVI CONTENTS
PART V ROOT ORGAN CULTURE OF OTHER FUNGAL SYMBIOSES
15
GEOSIPHON PYRIFORMIS
A GLOMEROMYCOTAN SOIL FUNGUS
FORMING ENDOSYMBIOSIS WITH CYANOBACTERIA 271
ARTHUR SCHUFILER, ELKE WOLF
1 INTRODUCTIO
N 271
2 DEVELOPMENT AN
D STRUCTURE OF THE SYMBIOTIC CONSORTIU
M .
. 271
2.1 STRUCTURE OF TH
E MATURE SYMBIOSIS 271
2.2 SPECIFICITY OF PARTNE
R RECOGNITION AN
D DEVELOPMENT
OF THE SYMBIOSIS 274
3 ECOLOGY AND DISTRIBUTIO
N OF TH
E
GEOSIPHON
SYMBIOSIS 274
3.1 ECOLOGY OF THE
GEOSIPHON
SYMBIOSIS 274
3.2 REPORTS AN
D DISTRIBUTIO
N OF THE
GEOSIPHON
SYMBIOSIS
. 275
3.3 AN ECOLOGICAL NETWORK
BETWEEN FUNGI, CYANOBACTERIA AN
D PLANTS? 276
4 CULTURE SYSTEMS FOR TH
E
GEOSIPHON
SYMBIOSIS 277
4.1 LABORATORY CULTURE SYSTEMS 277
4.2 CULTURE SYSTEMS FOR MICROSCOPIC INVESTIGATIONS 278
4.3 SYNCHRONIZATION OF THE
NOSTOC
LIFE CYCLE 279
5 METABOLIC ASPECTS OF THE SYMBIOSIS 280
5.1 C AN
D N METABOLISM 280
5.2 UPTAKE AN
D CONTENT OF INORGANIC NUTRIENT
S 281
5.3 HEAVY METAL UPTAKE AN
D RESISTANCE 282
6 PHYLOGENY AN
D TAXONOMY OF
GEOSIPHON
AN
D AM FUNGI 282
6.1
GEOSIPHON
IS AN 'AM FUNGUS
' 282
6.2 THE ORIGIN AN
D EVOLUTION
OF AM FUNGI AN
D THE AM SYMBIOSIS 284
7 THE
GEOSIPHON
SYMBIOSIS - A MODEL SYSTEM FOR AM? 285
7.1 PARTNE
R RECOGNITION AN
D SYMBIOSIS ESTABLISHMENT 285
7.2 BACTERIAL ENDOSYMBIONTS (BLOS) 285
7.3 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED
FUNGAL GENES 286
8 CONCLUSIONS 287
REFERENCES 287
16 SEBACINACEAE: CULTURABLE MYCORRHIZA-LIKE ENDOSYMBIOTIC FUNGI
AND THEIR INTERACTION
WITH NON-TRANSFORMED AND TRANSFORMED ROOTS 291
RAM PRASAD, HUONG GIANGPHAM, RINA KUMARI, ANJANA SINGH,
VIKAS YADAV, MINU SACHDEV, AMAR PRAKASH GARG, TATJANA PESKAN,
SOLVEIGHEHL, IRENA SHERAMETI, RALF OELMULLER, AJIT VARMA
1 INTRODUCTIO
N 291
CONTENTS XVII
2 SEBACINACEOUS FUNGI 292
3 HOST RANGE AN
D GROWTH PROMOTIO
N EFFECT
OF SEBACINACEOUS FUNGI 294
4 ECO-FUNCTIONAL IDENTITY 294
5 AXENIC CULTIVATION 298
6 MONOXENIC CULTURE 300
7 INTERACTIO
N WITH TRANSFORMED ROOTS 300
8 INTERACTIO
N WITH NON-TRANSFORMED ROOTS 303
9 CONCLUSION 309
REFERENCES 310
PART VI BIOTECHNOLOGY
17 LARGE-SCALE INOCULUM PRODUCTION OF ARBUSCULAR MYCORRHIZAL FUNGI
ON ROOT ORGANS AND INOCULATION STRATEGIES 315
ALOK ADHOLEYA, PRAGATI TIWARI, REENA SINGH
1 INTRODUCTIO
N 315
2 MONOXENIC-BASED AM INOCULUM PRODUCTIO
N 316
3 FORMULATIONS 318
4 INOCULATION STRATEGIES AN
D APPLICATION TECHNOLOGY 319
5 APPLICATION TECHNOLOGY 320
5.1 BROADCASTING 321
5.2 IN-FURROW APPLICATION 322
5.3 SEED DRESSING 322
5.4 ROOT DIPPING 322
5.5 SEEDLING INOCULATION 323
6 FIELD EVALUATION 323
6.1 FACTORS AFFECTING TH
E FIELD INOCULATION 326
7 RESPONSIBILITY OF TH
E SCIENTIFIC COMMUNIT
Y
TOWARDS TECHNOLOGY DEVELOPMENT 327
7.1 QUALITY CONTROL AND BENCHMARKING 328
8 SCOPES AN
D APPLICATIONS OF MONOXENICALLY BASED
AM FUNGAL TECHNOLOGY 329
8.1 CONSORTIU
M DEVELOPMENT OF AM FUNGI 329
8.2 HOST-BASED ENHANCED PRODUCTIVITY
IN AM FUNGUS PRODUCTIO
N 330
8.3 MYCORRHIZAE AN
D COUPLING BENEFICIAL ORGANISMS:
COMPLETE PACKAGE FOR ORGANIC SYSTEMS 330
9 POTENTIAL TOOL FOR ORGANIC FARMING 332
10 CONCLUSIONS 332
REFERENCES 333
XVIII CONTENTS
PART VII METHODOLOGY
18 METHODOLOGIES FOR IN VITRO CULTIVATION
OF ARBUSCULAR MYCORRHIZAL FUNGI WITH ROOT ORGANS 341
SYLVIE CRANENBROUCK, LIESBETH VOETS, CELINE BIVORT,
LAURENT RENARD, DESIRE-GEORGES STRULLU, STEPHANE DEDERCK
1 INTRODUCTIO
N 341
2 PROCESS DESCRIPTION 342
3 SELECTION OF TH
E CULTURE SYSTEM 342
4 CULTURE MEDIA PREPARATIO
N 347
4.1 MATERIAL 349
4.2 STOCK SOLUTIONS FOR MSR MEDIUM 349
4.3 MEDIUM PREPARATIO
N 350
5 HOST ROOT 351
5.1 CHOICE OF HOST ROOT 351
5.2 HOST ROOT TRANSFORMATION 353
5.3 HOST ROOT CULTIVATION 354
6 AM FUNGAL PROPAGULES: SELECTION AN
D DISINFECTION 356
6.1 SELECTION OF PROPAGULE 356
6.2 DISINFECTION PROCESS 357
7 MONOXENIC CULTURE ESTABLISHMENT 365
7.1 GERMINATION OF DISINFECTED PROPAGULES 365
7.2 MATERIAL 366
7.3 ASSOCIATION METHODS 366
8 CONTINUOUS CULTURE 368
8.1 ASSOCIATION ESTABLISHMENT 368
8.2 CONTINUOUS CULTURE 368
8.3 MATERIAL 369
8.4 SOLUTIONS 369
8.5 FIRST METHOD: PROPAGULE RE-ASSOCIATION 370
8.6 SECOND METHOD: MYCORRHIZED-APEX TRANSFER 371
9 CONCLUSION 372
REFERENCES 372
SUBJECT INDEX 377 |
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| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV022521462 |
| classification_rvk | WN 5200 WL 4385 |
| classification_tum | BIO 260f BIO 320f LAN 182f |
| collection | ZDB-2-SBL |
| ctrlnum | (OCoLC)762188025 (DE-599)BVBBV022521462 |
| discipline | Biologie Agrarwissenschaft Pflanzenbau |
| discipline_str_mv | Biologie Agrarwissenschaft Pflanzenbau |
| doi_str_mv | 10.1007/b138925 |
| format | Electronic eBook |
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV022521462 |
| illustrated | Not Illustrated |
| index_date | 2024-07-02T18:03:26Z |
| indexdate | 2024-07-09T20:59:25Z |
| institution | BVB |
| isbn | 9783540240273 9783540273318 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015728189 |
| oclc_num | 762188025 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource |
| psigel | ZDB-2-SBL |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Springer |
| record_format | marc |
| series | Soil biology |
| series2 | Soil biology |
| spelling | In vitro culture of mycorrhizas Stéphane Declerck ... (ed.) Berlin [u.a.] Springer 2005 1 Online-Ressource txt rdacontent c rdamedia cr rdacarrier Soil biology 4 Mykorrhiza (DE-588)4040983-1 gnd rswk-swf In-vitro-Kultur (DE-588)4332883-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mykorrhiza (DE-588)4040983-1 s In-vitro-Kultur (DE-588)4332883-0 s DE-604 Declerck, Stéphane Sonstige oth Erscheint auch als Druck-Ausgabe, Hardcover 3-540-24027-6 Soil biology 4 (DE-604)BV019353150 4 https://doi.org/10.1007/b138925 Verlag Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015728189&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | In vitro culture of mycorrhizas Soil biology Mykorrhiza (DE-588)4040983-1 gnd In-vitro-Kultur (DE-588)4332883-0 gnd |
| subject_GND | (DE-588)4040983-1 (DE-588)4332883-0 (DE-588)4143413-4 |
| title | In vitro culture of mycorrhizas |
| title_auth | In vitro culture of mycorrhizas |
| title_exact_search | In vitro culture of mycorrhizas |
| title_exact_search_txtP | In vitro culture of mycorrhizas |
| title_full | In vitro culture of mycorrhizas Stéphane Declerck ... (ed.) |
| title_fullStr | In vitro culture of mycorrhizas Stéphane Declerck ... (ed.) |
| title_full_unstemmed | In vitro culture of mycorrhizas Stéphane Declerck ... (ed.) |
| title_short | In vitro culture of mycorrhizas |
| title_sort | in vitro culture of mycorrhizas |
| topic | Mykorrhiza (DE-588)4040983-1 gnd In-vitro-Kultur (DE-588)4332883-0 gnd |
| topic_facet | Mykorrhiza In-vitro-Kultur Aufsatzsammlung |
| url | https://doi.org/10.1007/b138925 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015728189&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV019353150 |
| work_keys_str_mv | AT declerckstephane invitrocultureofmycorrhizas |