Salmonella methods and protocols

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Sprache:English
Veröffentlicht: Totowa, NJ Humana Press 2007
Schriftenreihe:Methods in molecular biology 394
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Datensatz im Suchindex

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adam_text Contents Preface .................................................................... v Contributors ............................................................... ix 1 Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex® xMAP™ Suspension Array Sherry A. Dunbar and James W. Jacobson ......................... 1 2 Detection of Salmonella by Bacteriophage Felix 01 Jonathan G Kuhn................................................. 21 3 Physical Mapping of Salmonella Genomes Shu-Lin Liu ....................................................... 39 4 Cloth-Based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella Burton W. Biais and Martine Gauthier............................ 59 5 Genome-Based Identification and Molecular Analyses of Pathogenicity Islands and Genomic Islands in Salmonella enterica Michael Hensel ................................................... 77 6 Determination of the Gene Content of Salmonella Genomes by Microarray Analysis Steffen Porwollik and Michael McClelland ........................ 89 7 In Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large Bacterial DNA Segments Using VEX-Capture James W. Wilson and Cheryl A. Nickerson ........................ í 05 8 Amplified Fragment Length Polymorphism Analysis oí Salmonella enterica Ruiting Lan and Peter R. Reeves .................................. 119 9 Salmonella Phages and Prophages: Cenomics and Practical Aspects Andrew M. Kropinski, Alexander Sulakvelidze, Paulina Kończy, and Cornelius Poppe ........................................... 133 VII v/// Contents 1 0 Salmonella Typhimurium Phage Typing for Pathogens Wolfgang Rabsch ................................................. 177 11 Salmonella Phages Examined in the Electron Microscope Hans-W. Ackermann............................................. 213 12 Applications of Cell Imaging in Salmonella Research Charlotte A. Perrett and Mark A. Jepson .......................... 235 13 Analysis of Kinesin Accumulation on Sa/moneffa-Containing Vacuoles Audrey Dumont, Nina Schroeder, Jean-Pierre Gorvel, and Stéphane Méresse......................................... 275 14 Magnesium, Manganese, and Divalent Cation Transport Assays in Intact Cells Michael E. Maguire ............................................... 289 15 Methods in Cell-to-Cell Signaling in Salmonella Brian M. M. Ahmer, ¡enee N. Smith, Jessica L Dyszel, and Amber Lindsay ............................................ 307 16 Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines Abraham Eisenstark, Robert A. Kazmierczak, Alison Dino, Rula Khreis, Dustin Newman, and Heide Schatten.............. 323 17 Further Resources for Molecular Protocols in Salmonella Research Abraham Eisenstark and Kelly K. Edwards ......................... 355 Index ......................................................................359 Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Safrnoneffa-specific cell antigens, and Salmonella-bost cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities for using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in generai. Because no recent comprehensive literature in this format is available on Salmonella, Salmonella: Methods and Protocols will be of value to a wide variety of researchers engaged in the study of Salmonella, and enierobacteria in general. FEATURES The first comprehensive collection of Salmonella methods and protocols Explains basic techniques that can be easily applied to any enterobacteria Each chapter includes a short overview followed by detailed explanations of techniques CONTENTS Quantitative, Multiplexed Detection oí Salmonella and Other Pathogens by Luminex® xMAP1 Suspension Array. Detection of Salmonella by Bacteriophage Felix 01. Physical Mapping of Salmonella Genomes. Cloth-Based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella. Genome-Based Identification and Molecular Analyses of Pathoge- nicity Islands and Genomic Islands in Salmonella enterica. Determination of the Gene Content of Salmonella Genomes by Microarray Analysis. In Vivo Excision, Cloning, and Broad-Host- Range Transfer of Large Bacterial DNA Segments Using VEX-Capture. Amplified Fragment Length Polymorphism Analysis of Salmonella enterica. Salmonella Phages and Prophages: Cenomics and Practical Aspects. Salmonella Typhimurium Phage Typing for Pathogens. Salmonella Phages Examined in the Electron Microscope. Applications of Cell Imaging in Salmonella Research. Analysis of Kinesin AccumulationonSa/mone/ia-Containing Vacuoles. Magnesium, Manganese, and Divalent Cation Transport Assays in Intact Cells. Methods in Cell-to- Cell Signaling in Salmonella. Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines. Further Resources for Molecular Protocols in Salmonella Research. Index.
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series2 Methods in molecular biology
spellingShingle Salmonella methods and protocols
Methods in molecular biology
Salmonella
Salmonella Laboratory Manuals
Salmonella Research
Salmonella (DE-588)4178963-5 gnd
Molekularbiologie (DE-588)4039983-7 gnd
subject_GND (DE-588)4178963-5
(DE-588)4039983-7
title Salmonella methods and protocols
title_auth Salmonella methods and protocols
title_exact_search Salmonella methods and protocols
title_full Salmonella methods and protocols ed. by Heide Schatten ...
title_fullStr Salmonella methods and protocols ed. by Heide Schatten ...
title_full_unstemmed Salmonella methods and protocols ed. by Heide Schatten ...
title_short Salmonella
title_sort salmonella methods and protocols
title_sub methods and protocols
topic Salmonella
Salmonella Laboratory Manuals
Salmonella Research
Salmonella (DE-588)4178963-5 gnd
Molekularbiologie (DE-588)4039983-7 gnd
topic_facet Salmonella
Salmonella Laboratory Manuals
Salmonella Research
Molekularbiologie
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