Protein purification principles, high resolution methods, and applications
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
Hoboken, NJ
Wiley
2011
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Ausgabe: | 3. ed. |
Schriftenreihe: | Methods of biochemical analysis
54 |
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Online-Zugang: | Inhaltsverzeichnis |
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MARC
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245 | 1 | 0 | |a Protein purification |b principles, high resolution methods, and applications |c ed. by Jan-Christer Janson |
250 | |a 3. ed. | ||
264 | 1 | |a Hoboken, NJ |b Wiley |c 2011 | |
300 | |a XIV, 517 S. |b Ill., graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
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Datensatz im Suchindex
DE-BY-TUM_call_number | 0040/CHE 820f 2011 B 1575(3) 0302/CHE 820f 1989 A 6961(3) 1002/CHE 820f 2013 B 1123(3) 0202/CHE 820f 2013 B 2033(3) 1302/CHE 820f 2014 B 84(3) |
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adam_text | CONTENTS
PREFACE TO THE THIRD EDITION
vii
PREFACE TO THE SECOND EDITION
ix
PREFACE TO THE FIRST EDITION
xi
CONTRIBUTORS
xiii
PART I INTRODUCTION l
1
Introduction to Protein Purification
3
Bo Ersson, Lars
Rydén,
and
Jan-Christer Janson
PART II CHROMATOGRAPHY
23
2
Introduction to Chromatography
25
Jan-Christer Janson
and Jan Ake
Jönsson
3
Gel Filtration: Size Exclusion Chromatography
51
Lars Hagel
4
Ion Exchange Chromatography
93
Evert
Karlsson
and
Irwin Hirsh
5
High-Resolution Reversed-Phase Chromatography of Proteins
135
Sylvia Winkel
Pettersson
6 Hydrophobie
Interaction Chromatography
165
Kjell-Ove
Eriksson and Makonnen Belew
7
Immobilized Metal Ion Affinity Chromatography
183
Lennart Kågedal
8
Covalent Chromatography
203
Francisco Batista-Viera,
Lars
Rydén,
and
Jan Carlsson
9
Affinity Chromatography
221
Francisco Batista-Viera,
Jan-Christer Janson,
and
Jan Carlsson
vi
CONTENTS
10
Affinity Ligands from Chemical Combinatorial Libraries
259
Enrique
Corredano
and Herbert
Baumann
11
Affinity Ligands from Biological Combinatorial Libraries
269
Per-Ake
Nygren
PART III OTHER SEPARATION METHODS AND RELATED TECHNIQUES
279
12
Membrane Separations
281
Joachim K. Walter, Zuwei Jin,
Maik
W.
Jomitz, and
Uwe Gottschalk
13
Refolding of Inclusion Body Proteins from
E. coli
319
Zhiguo
Su,
Diannan
Lu,
and Zheng Liu
14
Purification of PEGylated Proteins
339
Conan J. Fee and James M. Van Alstine
PART IV ELECTROPHORESIS
363
15
Electrophoresis in Gels
365
Reiner
Westermeier
16
Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized
pH
Gradients
379
Pier Giorgio
Righetti, Elisa
Fasoli,
and
Sabina
Carla Righetti
17
Two-Dimensional Electrophoresis in Proteomics
411
Reiner
Westenneier und Angelika Görg
18 Protein Elution
and Blotting Techniques
441
Reiner
Westermeier
19
Capillary Electrophoretic Separations
451
Wolfgang Thorrmum
PART V SEPARATION METHOD OPTIMIZATION
487
20
High Throughput Screening Techniques in Protein Purification
489
Karol
M. Lacki and Eggert Brekkan
INDEX
507
|
adam_txt |
CONTENTS
PREFACE TO THE THIRD EDITION
vii
PREFACE TO THE SECOND EDITION
ix
PREFACE TO THE FIRST EDITION
xi
CONTRIBUTORS
xiii
PART I INTRODUCTION l
1
Introduction to Protein Purification
3
Bo Ersson, Lars
Rydén,
and
Jan-Christer Janson
PART II CHROMATOGRAPHY
23
2
Introduction to Chromatography
25
Jan-Christer Janson
and Jan Ake
Jönsson
3
Gel Filtration: Size Exclusion Chromatography
51
Lars Hagel
4
Ion Exchange Chromatography
93
Evert
Karlsson
and
Irwin Hirsh
5
High-Resolution Reversed-Phase Chromatography of Proteins
135
Sylvia Winkel
Pettersson
6 Hydrophobie
Interaction Chromatography
165
Kjell-Ove
Eriksson and Makonnen Belew
7
Immobilized Metal Ion Affinity Chromatography
183
Lennart Kågedal
8
Covalent Chromatography
203
Francisco Batista-Viera,
Lars
Rydén,
and
Jan Carlsson
9
Affinity Chromatography
221
Francisco Batista-Viera,
Jan-Christer Janson,
and
Jan Carlsson
vi
CONTENTS
10
Affinity Ligands from Chemical Combinatorial Libraries
259
Enrique
Corredano
and Herbert
Baumann
11
Affinity Ligands from Biological Combinatorial Libraries
269
Per-Ake
Nygren
PART III OTHER SEPARATION METHODS AND RELATED TECHNIQUES
279
12
Membrane Separations
281
Joachim K. Walter, Zuwei Jin,
Maik
W.
Jomitz, and
Uwe Gottschalk
13
Refolding of Inclusion Body Proteins from
E. coli
319
Zhiguo
Su,
Diannan
Lu,
and Zheng Liu
14
Purification of PEGylated Proteins
339
Conan J. Fee and James M. Van Alstine
PART IV ELECTROPHORESIS
363
15
Electrophoresis in Gels
365
Reiner
Westermeier
16
Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized
pH
Gradients
379
Pier Giorgio
Righetti, Elisa
Fasoli,
and
Sabina
Carla Righetti
17
Two-Dimensional Electrophoresis in Proteomics
411
Reiner
Westenneier und Angelika Görg
18 Protein Elution
and Blotting Techniques
441
Reiner
Westermeier
19
Capillary Electrophoretic Separations
451
Wolfgang Thorrmum
PART V SEPARATION METHOD OPTIMIZATION
487
20
High Throughput Screening Techniques in Protein Purification
489
Karol
M. Lacki and Eggert Brekkan
INDEX
507 |
any_adam_object | 1 |
any_adam_object_boolean | 1 |
author2 | Janson, Jan-Christer |
author2_role | edt |
author2_variant | j c j jcj |
author_facet | Janson, Jan-Christer |
building | Verbundindex |
bvnumber | BV022279561 |
classification_rvk | VK 8560 WC 4170 WD 5100 WD 5275 |
classification_tum | CHE 810f CHE 808f CHE 820f CHE 232f CHE 228f |
ctrlnum | (OCoLC)635209334 (DE-599)BVBBV022279561 |
discipline | Chemie / Pharmazie Biologie Chemie |
discipline_str_mv | Chemie / Pharmazie Biologie Chemie |
edition | 3. ed. |
format | Book |
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genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
genre_facet | Aufsatzsammlung |
id | DE-604.BV022279561 |
illustrated | Illustrated |
index_date | 2024-07-02T16:48:56Z |
indexdate | 2024-11-25T17:26:05Z |
institution | BVB |
isbn | 0471746614 9780471746614 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015489895 |
oclc_num | 635209334 |
open_access_boolean | |
owner | DE-91G DE-BY-TUM DE-355 DE-BY-UBR DE-20 DE-11 DE-703 DE-M49 DE-BY-TUM DE-B768 DE-91S DE-BY-TUM DE-29T |
owner_facet | DE-91G DE-BY-TUM DE-355 DE-BY-UBR DE-20 DE-11 DE-703 DE-M49 DE-BY-TUM DE-B768 DE-91S DE-BY-TUM DE-29T |
physical | XIV, 517 S. Ill., graph. Darst. |
publishDate | 2011 |
publishDateSearch | 2011 |
publishDateSort | 2011 |
publisher | Wiley |
record_format | marc |
series | Methods of biochemical analysis |
series2 | Methods of biochemical analysis |
spellingShingle | Protein purification principles, high resolution methods, and applications Methods of biochemical analysis Chromatographie (DE-588)4010153-8 gnd Trennverfahren (DE-588)4078395-9 gnd Elektrophorese (DE-588)4014373-9 gnd Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd |
subject_GND | (DE-588)4010153-8 (DE-588)4078395-9 (DE-588)4014373-9 (DE-588)4049250-3 (DE-588)4076388-2 (DE-588)4143413-4 |
title | Protein purification principles, high resolution methods, and applications |
title_auth | Protein purification principles, high resolution methods, and applications |
title_exact_search | Protein purification principles, high resolution methods, and applications |
title_exact_search_txtP | Protein purification principles, high resolution methods, and applications |
title_full | Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson |
title_fullStr | Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson |
title_full_unstemmed | Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson |
title_short | Protein purification |
title_sort | protein purification principles high resolution methods and applications |
title_sub | principles, high resolution methods, and applications |
topic | Chromatographie (DE-588)4010153-8 gnd Trennverfahren (DE-588)4078395-9 gnd Elektrophorese (DE-588)4014373-9 gnd Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd |
topic_facet | Chromatographie Trennverfahren Elektrophorese Reinigung Proteine Aufsatzsammlung |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015489895&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV002528856 |
work_keys_str_mv | AT jansonjanchrister proteinpurificationprincipleshighresolutionmethodsandapplications |