YAC protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
349 |
| Schlagworte: | |
| Online-Zugang: | Table of contents only Inhaltsverzeichnis |
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MARC
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| 245 | 1 | 0 | |a YAC protocols |c ed. by Alasdair MacKenzie |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a XV, 203 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 349 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Molecular cloning |x Methods | |
| 650 | 4 | |a Genetic vectors | |
| 650 | 4 | |a Yeast fungi |x Genetic engineering | |
| 650 | 4 | |a Saccharomyces cerevisiae | |
| 650 | 4 | |a Chromosomes, Artificial, Yeast | |
| 650 | 4 | |a Genetic Vectors | |
| 650 | 4 | |a Genetic Techniques | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| DE-BY-UBR_call_number | 91/WC 4150 M157(2) |
|---|---|
| DE-BY-UBR_katkey | 3780553 |
| DE-BY-UBR_location | 91 |
| DE-BY-UBR_media_number | 069033568845 |
| _version_ | 1822699129740984320 |
| adam_text | Contents
Preface v
Contributors xiii
1 Markers, Selection, and Media in Yeast Artificial
Chromosome Cloning
David Markie 1
2 Construction of Yeast Artificial Chromosome Libraries
From Pathogens and Nonmodel Organisms
Cecilia P. Sanchez and Michael Lanzer 13
3 Analysis and Screening of Yeast Artificial Chromosome
Libraries by Filter Hybridization
Cecilia P. Sanchez and Michael Lanzer 27
4 Analysis of Yeast Artificial Chromosome DNA by Restriction
Digestion, Southern Blotting Nucleic Acid Hybridization,
and Polymerase Chain Reaction
Sylvia Vasiliou and John Peter Quinn 33
5 Postgenomic Bioinformatic Analysis of Yeast
Artificial Chromosome Sequence
Kerry Miller, Scott Davidson, and Alasdair Mackenzie 41
6 Transformation of Yeast Using Bioactive Beads With
Surface Immobilized Yeast Artificial Chromosomes
Shigeki Kawakami, Satoshi Harashima, Akio Kobayashi,
and Kiichi Fukui 6 7
7 Modification and Amplification of Yeast Artificial
Chromosomes
Sanbing Shen, Anthony Harmar, and Nick Hastie 67
8 Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP
and FLP/FRT Site Specific Deletions and Inversions
Gabriela G. Loots 75
9 Selective Isolation of Large Chromosomal Regions
by Transformation Associated Recombination
Cloning for Structural and Functional Analysis
of Mammalian Genomes
Natalay Kouprina, Vladimir N. Noskov,
and Vladimir Larionov 85
xi
xii Contents
10 A Polymerase Chain Reaction Mediated Yeast Artificial
Chromosome Splitting Technology for Generating
Targeted Yeast Artificial Chromosomes Subclones
Yeon Hee Kim, Minetaka Sugiyama, Yoshinobu Kaneko,
Kiichi Fukui, Akio Kobayashi, and Satoshi Harashima 103
11 Genomic Reconstruction by Serial Mitotic Recombination
of Yeast Artificial Chromosomes
David Markie, Emma Jones, and Jiannis Ragoussis 117
12 Targeting Vector Construction by Yeast Artificial
Chromosome Modification
Peter J. Murray 127
13 Production of Yeast Artificial Chromosome Transgenic Mice
by Pronuclear Injection of One Cell Embryos
Alasdair Mackenzie 139
14 Generation of Yeast Artificial Chromosome Transgenic Mice
by Intracytoplasmic Sperm Injection
Pedro N. Moreira, Julio Pozueta, Patricia Giraldo,
Alfonso Gutierrez Adan, and Lluis Montoliu 151
15 Establishment of Cell Lines That Exhibit Correct Ontogenic
Stage Specific Gene Expression Profiles From Tissues
of Yeast Artificial Chromosome Transgenic Mice Using
Chemically Induced Growth Signals
C. Anthony Blau and Kenneth R. Peterson 163
16 Application of Yeast Artificial Chromosomes in Fluorescence
In Situ Hybridization
Thomas Liehr 175
1 7 Contribution of Yeast Artificial Chromosome Based
Physical Maps to the Final Assembly of the Trypanosoma
cruzi Genome
Marcia R. M. Santos, Roberto R. M. Barros,
Patricio E. Porcile, Mariano J. Levin,
and Jose Franco da Silveira 187
Index 199
|
| any_adam_object | 1 |
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| dewey-full | 660.6/5 |
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| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6/5 |
| dewey-search | 660.6/5 |
| dewey-sort | 3660.6 15 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie |
| edition | 2. ed. |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV021766084 |
| illustrated | Illustrated |
| indexdate | 2024-12-23T19:31:05Z |
| institution | BVB |
| isbn | 1588296121 1597451584 9781588296122 |
| language | English |
| lccn | 2006000445 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014979076 |
| oclc_num | 62885444 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-12 |
| owner_facet | DE-355 DE-BY-UBR DE-12 |
| physical | XV, 203 S. Ill., graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | YAC protocols Methods in molecular biology Molecular cloning Methods Genetic vectors Yeast fungi Genetic engineering Saccharomyces cerevisiae Chromosomes, Artificial, Yeast Genetic Vectors Genetic Techniques Methode (DE-588)4038971-6 gnd Genetik (DE-588)4071711-2 gnd Hefeartige Pilze (DE-588)4230509-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4071711-2 (DE-588)4230509-3 (DE-588)4143413-4 |
| title | YAC protocols |
| title_auth | YAC protocols |
| title_exact_search | YAC protocols |
| title_full | YAC protocols ed. by Alasdair MacKenzie |
| title_fullStr | YAC protocols ed. by Alasdair MacKenzie |
| title_full_unstemmed | YAC protocols ed. by Alasdair MacKenzie |
| title_short | YAC protocols |
| title_sort | yac protocols |
| topic | Molecular cloning Methods Genetic vectors Yeast fungi Genetic engineering Saccharomyces cerevisiae Chromosomes, Artificial, Yeast Genetic Vectors Genetic Techniques Methode (DE-588)4038971-6 gnd Genetik (DE-588)4071711-2 gnd Hefeartige Pilze (DE-588)4230509-3 gnd |
| topic_facet | Molecular cloning Methods Genetic vectors Yeast fungi Genetic engineering Saccharomyces cerevisiae Chromosomes, Artificial, Yeast Genetic Vectors Genetic Techniques Methode Genetik Hefeartige Pilze Aufsatzsammlung |
| url | http://www.loc.gov/catdir/toc/ecip066/2006000445.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014979076&sequence=000006&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT mackenziealasdair yacprotocols |