Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
Fachverl. Köhler
2004
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| Schriftenreihe: | Agrarwissenschaft
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| 100 | 1 | |a Ibeagha-Awemu, Eveline Mengwi |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria |c eingereicht von Eveline Mengwi Ibeagha-Awemu |
| 264 | 1 | |a Gießen |b Fachverl. Köhler |c 2004 | |
| 300 | |a XIII, 208 S. |b Ill., graph. Darst., Kt. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Agrarwissenschaft | |
| 502 | |a Zugl.: Gießen, Univ., Diss., 2004 | ||
| 650 | 4 | |a Cattle breeds |z Cameroon | |
| 650 | 4 | |a Cattle breeds |z Nigeria | |
| 650 | 4 | |a Cattle |x Microbiology |z Cameroon | |
| 650 | 4 | |a Cattle |x Microbiology |z Nigeria | |
| 650 | 4 | |a Cattle |z Cameroon |x Genetics | |
| 650 | 4 | |a Cattle |z Nigeria |x Genetics | |
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Datensatz im Suchindex
| _version_ | 1804135112302919680 |
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| adam_text | AUS DEM INSTITUT FUR TIERZUCHT UND HAUSTIERGENETIK DER
JUSTUS-LIEBIG-UNIVERSITAT GIESSEN BETREUER: PROF. DR. G. ERHARDT
BIOCHEMICAL AND MOLECULAR GENETIC CHARACTERIZATION OF CATTLE BREEDS OF
CAMEROON AND NIGERIA INAUGURAL-DISSERTATION ZUR ERLANGUNG DES
DOKTORGRADES DR. AGR. BEIM FACHBEREICH AGRARWISSENSCHAFTEN,
OKOTROPHOLOGIE UND UMWELTMANAGEMENT DER JUSTUS-LIEBIG-UNIVERSITAT
GIESSEN EINGEREICHT VON EVELINE MENGWI IBEAGHA-AWEMU AUS BAMBILI,
KAMERUN GIESSEN, 2003 CONTENTS CONTENT PAGE LIST OF TABLES V LIST OF
FIGURES VIII APPENDICES X ABBREVIATIONS XI 1 INTRODUCTION 1 2 LITERATURE
REVIEW 3 2.1 THE ORIGIN AND DOMESTICATION OF AFRICAN CATTLE BREEDS 3 2.2
BLOOD PROTEINS BIOCHEMICAL POLYMORPHISMS 7 2.2.1 ALBUMIN (ALB) 8 2.2.2
CARBONIC ANHYDRASE (CA II) 8 2.2.3 VITAMIN D-BINDING PROTEIN (GC) 10
2.2.4 HAEMOGLOBIN (HBB) 11 2.2.5 TRANSFERRIN (TF) 13 2.3 MILK PROTEIN
GENES 16 2.3.1 STRUCTURE, ORGANIZATION AND EVOLUTION OF MILK PROTEIN
GENES 16 2.3.1.1 THE CASEIN GENES 16 2.3.1.2 THE MAJOR WHEY PROTEIN
GENES 19 2.3.2 GENETIC POLYMORPHISM OF MILK PROTEIN GENES 21 2.3.2.1 A S
I-CASEIN(CSNLSL) 21 2.3.2.2 CC S 2-CASEIN(CSNLS2) 24 2.3.2.3 P-CASEIN
(CSN2) 26 2.3.2.4 K-CASEIN (CSN3) 27 2.3.2.5 A-IACTALBUMIN (LAA) 29
2.3.2.6 P-LACTOGLOBULIN (LGB) 30 2.4 MICROSATELLITES 31 2.4.1 STRUCTURE
AND OCCURRENCE IN THE GENOME 31 2.4.2 RATES OF MICROSATELLITE MUTATIONS
34 2.4.3 CONSERVATION OF MICROSATELLITE LOCI BETWEEN SPECIES 34 2.4.4
MAJOR DRAWBACKS IN THE APPLICATION OF MICROSATELLITE MARKERS 35 2.5
UTILIZATION OF BLOOD/MILK PROTEIN AND MICROSATELLITE ALLELIC VARIANTS 35
2.5.1 PATERNITY INFERENCE AND TWIN DETERMINATION 35 2.5.2 MARKER ALLELIC
VARIANTS AND PRODUCTION, DISEASE AND ADAPTABILITY TRAITS 36 2.5.3 USE IN
DETERMINING THE RELATIONSHIPS BETWEEN BREEDS AND SPECIES 38 2.5.4
CONSTRUCTION OF GENETIC MAPS 39 2.5.5 DETECTION AND MAPPING OF
QUANTITATIVE TRAIT LOCI (QTL) 40 2.6 ANALYZING ANIMAL POPULATION
STRUCTURES 41 2.6.1 TESTING FOR DISEQUILIBRIUM 42 2.6.2 MEASURING
OBSERVED HETEROZYGOSITY AND GENETIC DIVERSITY 42 2.6.3 POPULATION
STRUCTURE 43 CONTENT PAGE 2.6.4 MEASURING GENE FLOW(NI;M) 43 2.6.5
ESTIMATING GENETIC ADMIXTURE 43 2.6.6 MEASURES OF GENETIC DISTANCES AND
CONSTRUCTION OF PHYLOGENIES 43 2.6.7 PRINCIPAL COMPONENT ANALYSIS (PCA)
OF ALLELE FREQUENCIES 44 3 MATERIALS 45 3.1 CONSUMABLES 45 3.2 CHEMICALS
45 3.3 REACTION KIDS 46 3.4 ENZYMES 46 3.5 NUCLEOTIDES 47 3.6 EQUIPMENT
47 3.7 SAMPLE COLLECTION 48 3.8 DISTRIBUTION AND CHARACTERISTICS OF
SAMPLED BREEDS 51 3.8.1 BOS INDICUS (ZEBU) BREEDS 51 3.8.1.1 RED BORORO
(RB) 51 3.8.1.2 WHITE FULANI (WF) 51 3.8.1.3 SOKOTO GUDALI (SO) 52
3.8.1.4 NGAOUNDERE GUDALI (NG) 53 3.8.1.5 BANYO GUDALI (BG) 53 3.8.1.6
ADAMAWA GUDALI (AG) 53 3.8.1.7 WADARA(WD) 54 3.8.2 BOS LAURUS BREEDS 54
3.8.2.1 NAMCHI(NA) 54 3.8.2.2 MUTURU(MT) 55 3.8.2.3 N DAMA(ND) 55 4
METHODS 56 4.1 PROTEIN ANALYSIS .. 56 4. .1 ANALYSIS OF BLOOD PROTEIN
VARIANTS 56 .2 DEMONSTRATION OF CA II, HBB AND TF VARIANTS BY PAG-IEF
. .. 56 .3 DEMONSTRATION OF GC AND TF VARIANTS BY PAGE 57 .4 ANALYSIS OF
MILK PROTEIN VARIANTS 58 .5 DEMONSTRATION OF MILK PROTEIN VARIANTS BY
PAG-IEF 58 .6 DEMONSTRATION OF WHEY PROTEIN VARIANTS BY PAGE 58 .7
HANDLING OF GELS AFTER PAG-IEF 59 .8 HANDLING OF GELS AFTER PAGE 59
4.1.9 PHENOTYPING 59 4.2 DEOXYRIBONUCLEIC ACID (DNA) ANALYSIS 61 4.2.1
DNA ISOLATION 61 4.2.2 QUANTIFICATION OF DNA 61 4.2.3 POLYMERASE CHAIN
REACTION (PCR) AMPLIFICATION OF GENOMIC DNA 62 4.2.4 AGAROSE GEL
ELECTROPHORESIS (AGE) 62 ILL CONTENT PAGE 4.2.5 RESTRICTION FRAGMENT
LENGTH POLYMORPHISM (RFLP) 63 4.2.6 SINGLE STRAND CONFORMATION
POLYMORPHISM (SSCP) 65 4.2.7 ALLELE SPECIFIC PCR 67 4.2.8 INTERNAL DNA
MARKERS USED DURING ELECTROPHORESIS 68 4.2.9 REFERENCE SEQUENCES USED 69
4.2.10 SEARCH FOR IDENTIFIED MUTATIONS ON CSN1 SI AND CSN1S2 GENES 69
4.2.11 PURIFICATION OF PCR PRODUCTS 70 4.2.12 CLONING OF PCR PRODUCTS 71
4.2.12.1 LIGATION OF PCR FRAGMENT IN VECTOR 71 4.2.12.2 TRANSFORMATION
71 4.2.12.3 ANALYSIS OF POSITIVE CLONES 72 4.2.13 MINI PREPARATION 72
4.2.14 SEQUENCING OF PCR PRODUCTS AND PLASMIDS 72 4.2.15 SEQUENCE
ANALYSIS 72 4.2.16 STAINING WITH ETHIDIUM BROMIDE 73 4.2.17 SILVER
STAINING 73 4.2.18 DOCUMENTATION 73 4.2.19 MICROSATELLITE ANALYSIS 74
4.2.19.1 CHOICE OF MICROSATELLITE MARKERS 74 4.2.19.2 PCR AMPLIFICATION
OF MICROSATELLITES 74 4.2.19.3 TYPING OF MICROSATELLITES 74 4.2.19.4
ANALYSIS OF MICROSATELLITE FRAGMENTS 76 4.3 STATISTICAL ANALYSIS 76
4.3.1 ALLELE/HAPLOTYPE FREQUENCIES 77 4.3.2 ESTIMATION OF GENETIC
ADMIXTURE PROPORTIONS 77 4.3.3 HARDY-WEINBERG EQUILIBRIUM (HWE) 77 4.3.4
GENOTYPIC DISEQUILIBRIUM (GED) 78 4.3.5 HETEROZYGOSITY AND GENE
DIVERSITY 78 4.3.6 POPULATION DIFFERENTIATION 78 4.3.7 GENE FLOW 78
4.3.8 GENETIC DISTANCES AND RELATIONSHIPS 79 4.3.9 PRINCIPAL COMPONENT
ANALYSIS 79 5 RESULTS 80 5.1 OBSERVED VARIANTS AND FREQUENCIES 80 5.1.1
BLOOD PROTEINS 80 5.1.1.1 ALBUMIN (ALB) 80 5.1.1.2 CARBONIC ANHYDRASE
(CA II) 80 5.1.1.3 VITAMIN D-BINDING PROTEIN (GC) 83 5.1.1.4 HAEMOGLOBIN
(HBB) 83 5.1.1.5 TRANSFERRIN (TF) 84 5.1.1.6 ALB/GC HAPLOTYPES AND
FREQUENCIES 84 5.1.2 MILK PROTEINS 88 5.1.2.1 MILK PROTEIN VARIANTS
DEMONSTRATED BY PAG-IEF AND PAGE 88 5.1.2.2 VARIANTS OF CSN1S2,LAA AND
LGB DEMONSTRATED BY PCR-RFLP 90 5.1.2.2.1 CSN1S2 90 5.1.2.2.2 LAA 91 IV
CONTENT PAGE 5.1.2.2.3 LGB 91 5.1.2.3 CSNISI F TESTED BY ALLELE SPECIFIC
PCR 93 5.1.2.4 VARIANTS OF CSNISLPROM, CSN1S1, CSN2 AND CSN3
DEMONSTRATED BY PCR-SSCP 93 5.1.2.4.1 CSNISLPROM 93 5.1.2.4.2 CSN1S1 94
5.1.2.4.3 CSN2 95 5.1.2.4.4 CSN3 96 5.1.2.5 CASEIN GENE HAPLOTYPES 97
5.1.2.6 SEQUENCE ANALYSIS OF CSN1S1 PROM 5 100 5.1.3 MICROSATELLITES 102
5.2 POPULATION ADMIXTURE 116 5.3 HARDY-WEINBERG EQUILIBRIUM (HWE) 118
5.4 GENOTYPIC DISEQUILIBRIUM 118 5.5 GENETIC DIVERSITY 121 5.5.1 NUMBER
OF ALLELES 121 5.5.2 OBSERVED HETEROZYGOSITY AND UNBIASED GENE DIVERSITY
123 5.6 POPULATION DIFFERENTIATION 124 5.7 GENE FLOW 126 5.8 GENETIC
DISTANCES AND PHYLOGENIES 128 5.9 PRINCIPAL COMPONENT ANALYSIS 134 6
DISCUSSION 136 6.1 BLOOD PROTEINS ALLELIC VARIATIONS 136 6.2 MILK
PROTEINS ALLELIC VARIATIONS 139 6.3 MICROSATELLITES ALLELIC VARIATIONS
143 6.4 GENETIC DIVERSITY 145 6.5 GENETIC INTROGRESSION J - 146 6.6
DISEQUILIBRIUM 147 6.7 POPULATION STRUCTURE AND GENE FLOW 148 6.8
RELATIONSHIPS 150 6.9 CONCLUSIONS AND IMPLICATIONS 152 7 SUMMARY 154 8
ZUSAMMENFASSUNG 158 9 REFERENCES 163 10 APPENDIX 202
|
| adam_txt |
AUS DEM INSTITUT FUR TIERZUCHT UND HAUSTIERGENETIK DER
JUSTUS-LIEBIG-UNIVERSITAT GIESSEN BETREUER: PROF. DR. G. ERHARDT
BIOCHEMICAL AND MOLECULAR GENETIC CHARACTERIZATION OF CATTLE BREEDS OF
CAMEROON AND NIGERIA INAUGURAL-DISSERTATION ZUR ERLANGUNG DES
DOKTORGRADES DR. AGR. BEIM FACHBEREICH AGRARWISSENSCHAFTEN,
OKOTROPHOLOGIE UND UMWELTMANAGEMENT DER JUSTUS-LIEBIG-UNIVERSITAT
GIESSEN EINGEREICHT VON EVELINE MENGWI IBEAGHA-AWEMU AUS BAMBILI,
KAMERUN GIESSEN, 2003 CONTENTS CONTENT PAGE LIST OF TABLES V LIST OF
FIGURES VIII APPENDICES X ABBREVIATIONS XI 1 INTRODUCTION 1 2 LITERATURE
REVIEW 3 2.1 THE ORIGIN AND DOMESTICATION OF AFRICAN CATTLE BREEDS 3 2.2
BLOOD PROTEINS BIOCHEMICAL POLYMORPHISMS 7 2.2.1 ALBUMIN (ALB) 8 2.2.2
CARBONIC ANHYDRASE (CA II) 8 2.2.3 VITAMIN D-BINDING PROTEIN (GC) 10
2.2.4 HAEMOGLOBIN (HBB) 11 2.2.5 TRANSFERRIN (TF) 13 2.3 MILK PROTEIN
GENES 16 2.3.1 STRUCTURE, ORGANIZATION AND EVOLUTION OF MILK PROTEIN
GENES 16 2.3.1.1 THE CASEIN GENES 16 2.3.1.2 THE MAJOR WHEY PROTEIN
GENES 19 2.3.2 GENETIC POLYMORPHISM OF MILK PROTEIN GENES 21 2.3.2.1 A S
I-CASEIN(CSNLSL) 21 2.3.2.2 CC S 2-CASEIN(CSNLS2) 24 2.3.2.3 P-CASEIN
(CSN2) 26 2.3.2.4 K-CASEIN (CSN3) 27 2.3.2.5 A-IACTALBUMIN (LAA) 29
2.3.2.6 P-LACTOGLOBULIN (LGB) 30 2.4 MICROSATELLITES 31 2.4.1 STRUCTURE
AND OCCURRENCE IN THE GENOME 31 2.4.2 RATES OF MICROSATELLITE MUTATIONS
34 2.4.3 CONSERVATION OF MICROSATELLITE LOCI BETWEEN SPECIES 34 2.4.4
MAJOR DRAWBACKS IN THE APPLICATION OF MICROSATELLITE MARKERS 35 2.5
UTILIZATION OF BLOOD/MILK PROTEIN AND MICROSATELLITE ALLELIC VARIANTS 35
2.5.1 PATERNITY INFERENCE AND TWIN DETERMINATION 35 2.5.2 MARKER ALLELIC
VARIANTS AND PRODUCTION, DISEASE AND ADAPTABILITY TRAITS 36 2.5.3 USE IN
DETERMINING THE RELATIONSHIPS BETWEEN BREEDS AND SPECIES 38 2.5.4
CONSTRUCTION OF GENETIC MAPS 39 2.5.5 DETECTION AND MAPPING OF
QUANTITATIVE TRAIT LOCI (QTL) 40 2.6 ANALYZING ANIMAL POPULATION
STRUCTURES 41 2.6.1 TESTING FOR DISEQUILIBRIUM 42 2.6.2 MEASURING
OBSERVED HETEROZYGOSITY AND GENETIC DIVERSITY 42 2.6.3 POPULATION
STRUCTURE 43 CONTENT PAGE 2.6.4 MEASURING GENE FLOW(NI;M) 43 2.6.5
ESTIMATING GENETIC ADMIXTURE 43 2.6.6 MEASURES OF GENETIC DISTANCES AND
CONSTRUCTION OF PHYLOGENIES 43 2.6.7 PRINCIPAL COMPONENT ANALYSIS (PCA)
OF ALLELE FREQUENCIES 44 3 MATERIALS 45 3.1 CONSUMABLES 45 3.2 CHEMICALS
45 3.3 REACTION KIDS 46 3.4 ENZYMES 46 3.5 NUCLEOTIDES 47 3.6 EQUIPMENT
47 3.7 SAMPLE COLLECTION 48 3.8 DISTRIBUTION AND CHARACTERISTICS OF
SAMPLED BREEDS 51 3.8.1 BOS INDICUS (ZEBU) BREEDS 51 3.8.1.1 RED BORORO
(RB) 51 3.8.1.2 WHITE FULANI (WF) 51 3.8.1.3 SOKOTO GUDALI (SO) 52
3.8.1.4 NGAOUNDERE GUDALI (NG) 53 3.8.1.5 BANYO GUDALI (BG) 53 3.8.1.6
ADAMAWA GUDALI (AG) 53 3.8.1.7 WADARA(WD) 54 3.8.2 BOS LAURUS BREEDS 54
3.8.2.1 NAMCHI(NA) 54 3.8.2.2 MUTURU(MT) 55 3.8.2.3 N'DAMA(ND) 55 4
METHODS 56 4.1 PROTEIN ANALYSIS . 56 4. .1 ANALYSIS OF BLOOD PROTEIN
VARIANTS 56 .2 DEMONSTRATION OF CA II, HBB AND TF VARIANTS BY PAG-IEF
.'. 56 .3 DEMONSTRATION OF GC AND TF VARIANTS BY PAGE 57 .4 ANALYSIS OF
MILK PROTEIN VARIANTS 58 .5 DEMONSTRATION OF MILK PROTEIN VARIANTS BY
PAG-IEF 58 .6 DEMONSTRATION OF WHEY PROTEIN VARIANTS BY PAGE 58 .7
HANDLING OF GELS AFTER PAG-IEF 59 .8 HANDLING OF GELS AFTER PAGE 59
4.1.9 PHENOTYPING 59 4.2 DEOXYRIBONUCLEIC ACID (DNA) ANALYSIS 61 4.2.1
DNA ISOLATION 61 4.2.2 QUANTIFICATION OF DNA 61 4.2.3 POLYMERASE CHAIN
REACTION (PCR) AMPLIFICATION OF GENOMIC DNA 62 4.2.4 AGAROSE GEL
ELECTROPHORESIS (AGE) 62 ILL CONTENT PAGE 4.2.5 RESTRICTION FRAGMENT
LENGTH POLYMORPHISM (RFLP) 63 4.2.6 SINGLE STRAND CONFORMATION
POLYMORPHISM (SSCP) 65 4.2.7 ALLELE SPECIFIC PCR 67 4.2.8 INTERNAL DNA
MARKERS USED DURING ELECTROPHORESIS 68 4.2.9 REFERENCE SEQUENCES USED 69
4.2.10 SEARCH FOR IDENTIFIED MUTATIONS ON CSN1 SI AND CSN1S2 GENES 69
4.2.11 PURIFICATION OF PCR PRODUCTS 70 4.2.12 CLONING OF PCR PRODUCTS 71
4.2.12.1 LIGATION OF PCR FRAGMENT IN VECTOR 71 4.2.12.2 TRANSFORMATION
71 4.2.12.3 ANALYSIS OF POSITIVE CLONES 72 4.2.13 MINI PREPARATION 72
4.2.14 SEQUENCING OF PCR PRODUCTS AND PLASMIDS 72 4.2.15 SEQUENCE
ANALYSIS 72 4.2.16 STAINING WITH ETHIDIUM BROMIDE 73 4.2.17 SILVER
STAINING 73 4.2.18 DOCUMENTATION 73 4.2.19 MICROSATELLITE ANALYSIS 74
4.2.19.1 CHOICE OF MICROSATELLITE MARKERS 74 4.2.19.2 PCR AMPLIFICATION
OF MICROSATELLITES 74 4.2.19.3 TYPING OF MICROSATELLITES 74 4.2.19.4
ANALYSIS OF MICROSATELLITE FRAGMENTS 76 4.3 STATISTICAL ANALYSIS 76
4.3.1 ALLELE/HAPLOTYPE FREQUENCIES 77 4.3.2 ESTIMATION OF GENETIC
ADMIXTURE PROPORTIONS 77 4.3.3 HARDY-WEINBERG EQUILIBRIUM (HWE) 77 4.3.4
GENOTYPIC DISEQUILIBRIUM (GED) 78 4.3.5 HETEROZYGOSITY AND GENE
DIVERSITY 78 4.3.6 POPULATION DIFFERENTIATION 78 4.3.7 GENE FLOW 78
4.3.8 GENETIC DISTANCES AND RELATIONSHIPS 79 4.3.9 PRINCIPAL COMPONENT
ANALYSIS 79 5 RESULTS 80 5.1 OBSERVED VARIANTS AND FREQUENCIES 80 5.1.1
BLOOD PROTEINS 80 5.1.1.1 ALBUMIN (ALB) 80 5.1.1.2 CARBONIC ANHYDRASE
(CA II) 80 5.1.1.3 VITAMIN D-BINDING PROTEIN (GC) 83 5.1.1.4 HAEMOGLOBIN
(HBB) 83 5.1.1.5 TRANSFERRIN (TF) 84 5.1.1.6 ALB/GC HAPLOTYPES AND
FREQUENCIES 84 5.1.2 MILK PROTEINS 88 5.1.2.1 MILK PROTEIN VARIANTS
DEMONSTRATED BY PAG-IEF AND PAGE 88 5.1.2.2 VARIANTS OF CSN1S2,LAA AND
LGB DEMONSTRATED BY PCR-RFLP 90 5.1.2.2.1 CSN1S2 90 5.1.2.2.2 LAA 91 IV
CONTENT PAGE 5.1.2.2.3 LGB 91 5.1.2.3 CSNISI F TESTED BY ALLELE SPECIFIC
PCR 93 5.1.2.4 VARIANTS OF CSNISLPROM, CSN1S1, CSN2 AND CSN3
DEMONSTRATED BY PCR-SSCP 93 5.1.2.4.1 CSNISLPROM 93 5.1.2.4.2 CSN1S1 94
5.1.2.4.3 CSN2 95 5.1.2.4.4 CSN3 96 5.1.2.5 CASEIN GENE HAPLOTYPES 97
5.1.2.6 SEQUENCE ANALYSIS OF CSN1S1 PROM 5 100 5.1.3 MICROSATELLITES 102
5.2 POPULATION ADMIXTURE 116 5.3 HARDY-WEINBERG EQUILIBRIUM (HWE) 118
5.4 GENOTYPIC DISEQUILIBRIUM 118 5.5 GENETIC DIVERSITY 121 5.5.1 NUMBER
OF ALLELES 121 5.5.2 OBSERVED HETEROZYGOSITY AND UNBIASED GENE DIVERSITY
123 5.6 POPULATION DIFFERENTIATION 124 5.7 GENE FLOW 126 5.8 GENETIC
DISTANCES AND PHYLOGENIES 128 5.9 PRINCIPAL COMPONENT ANALYSIS 134 6
DISCUSSION 136 6.1 BLOOD PROTEINS ALLELIC VARIATIONS 136 6.2 MILK
PROTEINS ALLELIC VARIATIONS 139 6.3 MICROSATELLITES ALLELIC VARIATIONS
143 6.4 GENETIC DIVERSITY 145 6.5 GENETIC INTROGRESSION J - 146 6.6
DISEQUILIBRIUM 147 6.7 POPULATION STRUCTURE AND GENE FLOW 148 6.8
RELATIONSHIPS 150 6.9 CONCLUSIONS AND IMPLICATIONS 152 7 SUMMARY 154 8
ZUSAMMENFASSUNG 158 9 REFERENCES 163 10 APPENDIX 202 |
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| author | Ibeagha-Awemu, Eveline Mengwi |
| author_facet | Ibeagha-Awemu, Eveline Mengwi |
| author_role | aut |
| author_sort | Ibeagha-Awemu, Eveline Mengwi |
| author_variant | e m i a emi emia |
| building | Verbundindex |
| bvnumber | BV021309432 |
| callnumber-first | S - Agriculture |
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| callnumber-subject | SF - Animal Culture |
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| ctrlnum | (OCoLC)63514283 (DE-599)BVBBV021309432 |
| discipline | Biologie Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| discipline_str_mv | Biologie Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| geographic | Nigeria (DE-588)4042300-1 gnd Kamerun (DE-588)4029413-4 gnd |
| geographic_facet | Nigeria Kamerun |
| id | DE-604.BV021309432 |
| illustrated | Illustrated |
| index_date | 2024-07-02T13:55:30Z |
| indexdate | 2024-07-09T20:35:19Z |
| institution | BVB |
| isbn | 3935713878 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014630006 |
| oclc_num | 63514283 |
| open_access_boolean | |
| owner | DE-703 |
| owner_facet | DE-703 |
| physical | XIII, 208 S. Ill., graph. Darst., Kt. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Fachverl. Köhler |
| record_format | marc |
| series2 | Agrarwissenschaft |
| spelling | Ibeagha-Awemu, Eveline Mengwi Verfasser aut Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria eingereicht von Eveline Mengwi Ibeagha-Awemu Gießen Fachverl. Köhler 2004 XIII, 208 S. Ill., graph. Darst., Kt. txt rdacontent n rdamedia nc rdacarrier Agrarwissenschaft Zugl.: Gießen, Univ., Diss., 2004 Cattle breeds Cameroon Cattle breeds Nigeria Cattle Microbiology Cameroon Cattle Microbiology Nigeria Cattle Cameroon Genetics Cattle Nigeria Genetics Populationsgenetik (DE-588)4046804-5 gnd rswk-swf Rinderrasse (DE-588)4126570-1 gnd rswk-swf Nigeria (DE-588)4042300-1 gnd rswk-swf Kamerun (DE-588)4029413-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Kamerun (DE-588)4029413-4 g Rinderrasse (DE-588)4126570-1 s Populationsgenetik (DE-588)4046804-5 s DE-604 Nigeria (DE-588)4042300-1 g HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014630006&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ibeagha-Awemu, Eveline Mengwi Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria Cattle breeds Cameroon Cattle breeds Nigeria Cattle Microbiology Cameroon Cattle Microbiology Nigeria Cattle Cameroon Genetics Cattle Nigeria Genetics Populationsgenetik (DE-588)4046804-5 gnd Rinderrasse (DE-588)4126570-1 gnd |
| subject_GND | (DE-588)4046804-5 (DE-588)4126570-1 (DE-588)4042300-1 (DE-588)4029413-4 (DE-588)4113937-9 |
| title | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria |
| title_auth | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria |
| title_exact_search | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria |
| title_exact_search_txtP | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria |
| title_full | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria eingereicht von Eveline Mengwi Ibeagha-Awemu |
| title_fullStr | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria eingereicht von Eveline Mengwi Ibeagha-Awemu |
| title_full_unstemmed | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria eingereicht von Eveline Mengwi Ibeagha-Awemu |
| title_short | Biochemical and molecular genetic characterization of cattle breeds of Cameroon and Nigeria |
| title_sort | biochemical and molecular genetic characterization of cattle breeds of cameroon and nigeria |
| topic | Cattle breeds Cameroon Cattle breeds Nigeria Cattle Microbiology Cameroon Cattle Microbiology Nigeria Cattle Cameroon Genetics Cattle Nigeria Genetics Populationsgenetik (DE-588)4046804-5 gnd Rinderrasse (DE-588)4126570-1 gnd |
| topic_facet | Cattle breeds Cameroon Cattle breeds Nigeria Cattle Microbiology Cameroon Cattle Microbiology Nigeria Cattle Cameroon Genetics Cattle Nigeria Genetics Populationsgenetik Rinderrasse Nigeria Kamerun Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014630006&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ibeaghaawemuevelinemengwi biochemicalandmoleculargeneticcharacterizationofcattlebreedsofcameroonandnigeria |