Handbook of ELISPOT methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2005
|
| Schriftenreihe: | Methods in molecular biology
302 |
| Schlagworte: | |
| Online-Zugang: | Table of contents Klappentext Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a Handbook of ELISPOT |b methods and protocols |c ed. by Alexander E. Kalyuzhny |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2005 | |
| 300 | |a XIV, 321 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 302 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a ELISA | |
| 650 | 4 | |a Enzyme-linked immunosorbent assay | |
| 650 | 4 | |a Enzyme-Linked Immunosorbent Assay |x methods | |
| 650 | 4 | |a Cytokines |x immunology | |
| 650 | 4 | |a Interferon Type II |x immunology | |
| 650 | 4 | |a T-Lymphocytes |x immunology | |
| 650 | 0 | 7 | |a Enzyme-linked immunosorbent assay |0 (DE-588)4152476-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Enzyme-linked immunosorbent assay |0 (DE-588)4152476-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Kalyuzhny, Alexander E. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 302 |w (DE-604)BV035362695 |9 302 | |
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Datensatz im Suchindex
| _version_ | 1819685536630046720 |
|---|---|
| adam_text | Although the ELISPOT (enzyme-linked ¡mmunospot) assay is widely used in many fields of
icai
but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book
dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design,
perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety
of topics, including the use of membrane-backed plates, the standardization and validation procedures, the
removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data.
There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple
sclerosis, immune responses, multicytokine detection systems, and ¡mmunocytochemistry. Highlights in¬
clude dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standard¬
ization of the ELISPOT procedure, and combining the ELISPOT assay with ¡mmunohistochemistry. The
protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step
laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary
equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both
novice and experienced researchers a wealth of easy-to-understand information that will clarify the tech¬
nically challenging aspects of ELISPOT and open the door to its wider successful use.
How to successfully design and perform ELISPOT assay
and analyze experimental data system cells
Emphasis on the merits and shortcomings of different
ELISPOT techniques multiplex ELISPOT assays
Notes on how to modify ELISPOT technique to meet
certain experimental needs
State-of-the-art ELISPOT quantification techniques avoiding known pitfalls
Contents
Preface v
Contributors ix
Part I. ELISPOT Assay Setup and Data Analysis
1 The ELISPOT Assay: A Personal Retrospective
Jonathon D. Sedgwick 3
2 Chemistry and Biology of the ELISPOT Assay
Alexander E. Kalyuzhny 15
3 Membranes and Membrane Plates Used in ELISPOT
Alan J. Weiss 33
4 Standardization and Validation Issues of the ELISPOT Assay
Sylvia fanetzki, Josephine H. Cox, Neal Oden,
and Guido Ferrari 5 /
5 A Cell-Detachment Solution Can Reduce Background Staining
in the ELISPOT Assay
Angela Grant, Sarah Palzer, Chris Hartnett, Tanya Bailey,
Monica Tsang, and Alexander E. Kalyuzhny 87
6 Isolation of Subsets of Immune Cells
Carrie E. Peters, Steven M. Woodside, and Allen C. Eaves 95
7 Image Analysis and Data Management of ELISPOT
Assay Results
Paul Viktor Lehmann 117
8 High Resolution as a Key Feature to Perform Accurate ELISPOT
Measurements Using Zeiss KS ELISPOT Readers
Wolf Malkusch 133
Part II. ELISPOT Assay in Animal Models
9 Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific
Cellular Immune Responses in Rhesus Macaques
K. Jagannadha Sastry, Pramod N. Nehete, and Bharti Nehete 153
10 Feline Cytokine ELISPOT: Issues in Assay Development
Sushila K. Nordone, Rosemary Stevens, Alora S. LaVoy,
and Gregg A. Dean 167
vii
vjjj Contents
11 Measuring T-Cell Function in Animal Models of Tuberculosis
by ELISPOT
Vanja Lazarevic, Santosh Pawar, and JoAnne Flynn 179
12 Interferon-y ELISPOT Assay for the Quantitative Measurement
of Antigen-Specific Murine CD8+ T-Cells
Geoffrey A. Cole 191
Part Ml. ELISPOT Assays in Human Studies
1 3 Detection of Measles Virus-Specific Interferon-y-Secreting
T-Cells by ELISPOT
Jenna E. Ryan, Inna G. Ovsyannikova, and Gregory A. Poland 207
14 Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay
Clara M. Pelfrey and loana R. Moldovan 219
1 5 Use of Interferon-y ELISPOT in Monitoring Immune Responses
in Humans
Mark Matijevic and Robert G. Urban 237
16 ELISPOT Determination of Interferon-y T-CelI Frequencies
in Patients With Autoimmune Sensorineural Hearing Loss
C. Arturo Solares and Vincent K. Tuohy 253
Part IV. Multiplex and Modified ELISPOT Assay Formats
1 7 Dual-Color ELISPOT Assay for Analyzing Cytokine Balance
Yoshihiro Okamoto and Mikio Nishida 263
18 Simultaneous Detection of Multiple Cytokines in ELISPOT Assays
Sarah Palzer, Tanya Bailey, Chris Hartnett, Angela Grant,
Monica Tsang, and Alexander E. Kalyuzhny 273
19 Fluorospot Assay: Methodological Analysis
Agnes Gazagne, Wolf Malkusch, Benoit Vingert,
Wolf H. Fridman, and Eric Tartour 289
20 A Gel-Based Dual Antibody Capture and Detection Method
for Assaying of Extracellular Cytokine Secretion: EHCell
Lisa A. Spencer, Rossana C. N. Melo, Sandra A. C. Perez,
and Peter F. Weller 297
Index 315
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV019814520 |
| callnumber-first | Q - Science |
| callnumber-label | QP519 |
| callnumber-raw | QP519.9.E48 |
| callnumber-search | QP519.9.E48 |
| callnumber-sort | QP 3519.9 E48 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4150 WC 4350 YV 4500 |
| ctrlnum | (OCoLC)56013074 (DE-599)BVBBV019814520 |
| dewey-full | 616.07/9 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.07/9 |
| dewey-search | 616.07/9 |
| dewey-sort | 3616.07 19 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV019814520 |
| illustrated | Illustrated |
| indexdate | 2024-12-23T18:16:21Z |
| institution | BVB |
| isbn | 1588294692 |
| language | English |
| lccn | 2004016928 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-013139887 |
| oclc_num | 56013074 |
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| owner_facet | DE-12 DE-355 DE-BY-UBR |
| physical | XIV, 321 S. Ill., graph. Darst. |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | Handbook of ELISPOT methods and protocols Methods in molecular biology ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay methods Cytokines immunology Interferon Type II immunology T-Lymphocytes immunology Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd |
| subject_GND | (DE-588)4152476-7 (DE-588)4143413-4 |
| title | Handbook of ELISPOT methods and protocols |
| title_auth | Handbook of ELISPOT methods and protocols |
| title_exact_search | Handbook of ELISPOT methods and protocols |
| title_full | Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny |
| title_fullStr | Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny |
| title_full_unstemmed | Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny |
| title_short | Handbook of ELISPOT |
| title_sort | handbook of elispot methods and protocols |
| title_sub | methods and protocols |
| topic | ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay methods Cytokines immunology Interferon Type II immunology T-Lymphocytes immunology Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd |
| topic_facet | ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay methods Cytokines immunology Interferon Type II immunology T-Lymphocytes immunology Aufsatzsammlung |
| url | http://www.loc.gov/catdir/toc/ecip0420/2004016928.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013139887&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=013139887&sequence=000006&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
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