Germ cell protocols 1 Sperm and oocyte analysis

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Veröffentlicht: Totowa, N.J. Humana Press 2004
Schriftenreihe:Methods in molecular biology 253
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adam_text Contents Preface v Contributors ix Contents for Volume 2 xiii 1 Cryopreservation of Semen of the Salmonidae with Special Reference to Large Scale Fertilization Franz Lahnsteiner 1 2 Fertilization and Sperm Chemotaxis in Ascidians Manabu Yoshida 13 3 Assays for Vertebrate Sperm Chemotaxis Hitoshi Sugiyama, Bader Al Anzi, Robert W. McGaughey, and Douglas E. Chandler 27 4 Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods Katrina Merkies and Mary M. Buhr 49 5 Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy Peter Sutovsky 59 6 Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore Conjugated Solubilized Zona Pellucida Proteins Heather R. Burkin, Ana Paula Alves Vieira, and David J. Miller.... 79 7 Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction Olufemi A. Olatunbosun, Allison M. Case, and Harry G. Deneer.. 95 8 Spermatogonial Stem Cells Karim Nayernia, Manyu Li, and Wolfgang Engel 105 9 Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System Karim Nayernia and Wolfgang Engel 121 10 Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction Yehudit Lax, Sara Rubinstein, and Haim Breitbart 135 vii viii Contents 11 Isolation of Sea Urchin Sperm Plasma Membranes Kathryn J. Mengerink and Victor D. Vacquier 141 12 Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy Heide Schatten and Amitabha Chakrabarti 151 13 Microscopic Techniques for Studying Sperm Oocyte Interaction During Fertilization and Early Embryo Development Mark W. Tengowski 165 14 Immunocytochemical Staining of the Metaphase II Spindle in Mammalian Oocytes Steve Mullen and John K. Critser 201 15 Spindle Imaging in Living Mammalian Oocytes Using the Liquid Crystal Polarized Light Microscope and Its Practical Use Wei Hua Wang, Jimmy Gill, and Cathy Boutin 215 16 In Vitro Maturation and Fertilization of Pig Oocytes Heng Yu Fan and Qing Yuan Sun 227 17 In Vitro Maturation and In Vitro Fertilization of Human Oocytes Wei Hua Wang and Jimmy Gill 235 18 In Vitro Maturation and Fertilization of Canine Oocytes Karine Reynaud, Marie Saint Dizier, and Sylvie Chastant Maillard 255 19 Detection and Measurement of Membrane Bound Protein Tyrosine Kinases in the Zebrafish Egg Wenjun Wu and William H. Kinsey 273 20 A Single Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg Elinor MacGregor and William H. Kinsey 285 21 Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs Qing Yuan Sun and Heng Yu Fan 293 Index 305
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spellingShingle Germ cell protocols
Methods in molecular biology
Gamet (DE-588)4157017-0 gnd
Labortechnik (DE-588)4123602-6 gnd
subject_GND (DE-588)4157017-0
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title Germ cell protocols
title_auth Germ cell protocols
title_exact_search Germ cell protocols
title_full Germ cell protocols 1 Sperm and oocyte analysis Ed. by Heide Schatten
title_fullStr Germ cell protocols 1 Sperm and oocyte analysis Ed. by Heide Schatten
title_full_unstemmed Germ cell protocols 1 Sperm and oocyte analysis Ed. by Heide Schatten
title_short Germ cell protocols
title_sort germ cell protocols sperm and oocyte analysis
topic Gamet (DE-588)4157017-0 gnd
Labortechnik (DE-588)4123602-6 gnd
topic_facet Gamet
Labortechnik
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012829709&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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