Directed evolution library creation methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2003
|
| Schriftenreihe: | Methods in molecular biology
231 |
| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis |
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MARC
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| 245 | 1 | 0 | |a Directed evolution library creation |b methods and protocols |c edited by Frances H. Arnold and George Georgiou |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2003 | |
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| 490 | 1 | |a Methods in molecular biology |v 231 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Aufsatzsammlung - Proteindesign | |
| 650 | 4 | |a Combinatorial Chemistry Techniques | |
| 650 | 4 | |a Combinatorial chemistry |v Laboratory manuals | |
| 650 | 4 | |a Gene Library | |
| 650 | 4 | |a Gene libraries |v Laboratory manuals | |
| 650 | 4 | |a Genetic engineering |v Laboratory manuals | |
| 650 | 4 | |a Genetic recombination |v Laboratory manuals | |
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 1002 BIO 180f 2006 A 2696 |
|---|---|
| DE-BY-TUM_katkey | 1539276 |
| DE-BY-TUM_location | 10 |
| DE-BY-TUM_media_number | 040050463728 |
| DE-BY-UBR_call_number | 00/WC 4150 W181 |
| DE-BY-UBR_katkey | 3514122 |
| DE-BY-UBR_location | 00 |
| DE-BY-UBR_media_number | 069030846459 |
| _version_ | 1822683105969831936 |
| adam_text |
Contents
Preface v
Contributors ix
Part I Mutagenesis and Recombination Methods
1 Generating Mutant Libraries Using Error Prone PCR
Patrick C. Cirino, Kimberly M. Mayer, and Daisuke Umeno 3
2 Preparing Libraries in Escherichia coli
Alexander V. Tobias 11
3 Preparing Libraries in Saccharomyces cerevisiae
Thomas Butter and Miguel Alcalde 17
4 Creating Random Mutagenesis Libraries by Megaprimer PCR
of Whole Plasmid (MEGAWHOP)
Kentaro Miyazaki 23
5 Construction of Designed Protein Libraries
Using Gene Assembly Mutagenesis
Paul H. Bessette, Marco A. Mena, Annalee W. Nguyen,
and Patrick S. Daugherty 29
6 Production of Randomly Mutated Plasmid Libraries
Using Mutator Strains
Annalee W. Nguyen and Patrick S. Daugherty 39
7 Evolution of Microorganisms Using Mutator Plasmids
Olga Sellfonova and Volker Schellenberger 45
8 Random Insertion and Deletion Mutagenesis
Hiroshi Murakami, Takahiro Hohsaka, and Masahiko Sisido 53
9 Random Oligonucleotide Mutagenesis
Jessica L. Sneeden and Lawrence A. Loeb 65
10 Saturation Mutagenesis
Radu Georgescu, Geethani Bandara, and Lianhong Sun 75
11 DNA Shuffling
Jo/7/7 M. Joern 85
12 Family Shuffling with Single Stranded DNA
Wenjuan Zha, TongboZhu, and Huimin Zhao 91
13 In Vitro DNA Recombination by Random Priming
Olga Esteban, Ryan D. Woodyer, and Huimin Zhao 99
vii
viii Contents
14 Staggered Extension Process (StEP) In Vitro Recombination
Anna Marie Aguinaldo and Frances H. Arnold 105
15 RACHITT: Gene Family Shuffling by Random Chimeragenesis
on Transient Templates
Wayne M. Coco 111
16 The Creation of ITCHY Hybrid Protein Libraries
Marc Ostermeier and Stefan Lutz 129
17 Preparation of SCRATCHY Hybrid Protein Libraries:
Size and In Frame Selection of Nucleic Acid Sequences
Stefan Lutz and Marc Ostermeier 143
18 Sequence Homology lndependent Protein Recombination (SHIPREC)
Andrew K. Udit, Jonathan J. Silberg, and Volker Sieber 153
19 Producing Chimeric Genes by CLERY:
In Vitro and In Vivo Recombination
Valerie Ab6cassis, Denis Pompon, and Gilles Truan 165
Part II Analysis of Library Diversity
20 Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization
Peter Meinhold, John M. Joern, and Jonathan J. Silberg 177
21 Sequence Mapping of Combinatorial Libraries
on Macro or Microarrays: Experimental Design of DNA Arrays
Valerie Abecassis, Gilles Truan, Loic Jaffrelo,
and Denis Pompon 189
22 Sequence Mapping of Combinatorial Libraries
on Macro and Microarrays: Bioinformatic Treatment of Data
Denis Pompon, Gilles Truan, and Valerie Ab6cassis 199
Index 213 |
| any_adam_object | 1 |
| author_GND | (DE-588)128524359 |
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| bvnumber | BV017087256 |
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| discipline | Chemie / Pharmazie Biologie |
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| genre_facet | Aufsatzsammlung Aufsatzsammlung - Genbibliothek - Genetische Variabilität - Bioverfahrenstechnik - Ortspezifische Mutagenese |
| id | DE-604.BV017087256 |
| illustrated | Not Illustrated |
| indexdate | 2025-01-02T18:34:01Z |
| institution | BVB |
| isbn | 1588292851 |
| language | English |
| lccn | 2003007889 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010306291 |
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| physical | X, 224 S. |
| publishDate | 2003 |
| publishDateSearch | 2003 |
| publishDateSort | 2003 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | Directed evolution library creation methods and protocols Methods in molecular biology Aufsatzsammlung - Proteindesign Combinatorial Chemistry Techniques Combinatorial chemistry Laboratory manuals Gene Library Gene libraries Laboratory manuals Genetic engineering Laboratory manuals Genetic recombination Laboratory manuals Microbial mutation Laboratory manuals Molecular evolution Laboratory manuals Genetische Variabilität (DE-588)4264352-1 gnd Proteindesign (DE-588)4346801-9 gnd Ortspezifische Mutagenese (DE-588)4312683-2 gnd Bioverfahrenstechnik (DE-588)4307166-1 gnd Genbibliothek (DE-588)4236160-6 gnd |
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| title | Directed evolution library creation methods and protocols |
| title_auth | Directed evolution library creation methods and protocols |
| title_exact_search | Directed evolution library creation methods and protocols |
| title_full | Directed evolution library creation methods and protocols edited by Frances H. Arnold and George Georgiou |
| title_fullStr | Directed evolution library creation methods and protocols edited by Frances H. Arnold and George Georgiou |
| title_full_unstemmed | Directed evolution library creation methods and protocols edited by Frances H. Arnold and George Georgiou |
| title_short | Directed evolution library creation |
| title_sort | directed evolution library creation methods and protocols |
| title_sub | methods and protocols |
| topic | Aufsatzsammlung - Proteindesign Combinatorial Chemistry Techniques Combinatorial chemistry Laboratory manuals Gene Library Gene libraries Laboratory manuals Genetic engineering Laboratory manuals Genetic recombination Laboratory manuals Microbial mutation Laboratory manuals Molecular evolution Laboratory manuals Genetische Variabilität (DE-588)4264352-1 gnd Proteindesign (DE-588)4346801-9 gnd Ortspezifische Mutagenese (DE-588)4312683-2 gnd Bioverfahrenstechnik (DE-588)4307166-1 gnd Genbibliothek (DE-588)4236160-6 gnd |
| topic_facet | Aufsatzsammlung - Proteindesign Combinatorial Chemistry Techniques Combinatorial chemistry Laboratory manuals Gene Library Gene libraries Laboratory manuals Genetic engineering Laboratory manuals Genetic recombination Laboratory manuals Microbial mutation Laboratory manuals Molecular evolution Laboratory manuals Genetische Variabilität Proteindesign Ortspezifische Mutagenese Bioverfahrenstechnik Genbibliothek Aufsatzsammlung Aufsatzsammlung - Genbibliothek - Genetische Variabilität - Bioverfahrenstechnik - Ortspezifische Mutagenese |
| url | http://www.loc.gov/catdir/toc/fy037/2003007889.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010306291&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT arnoldfranceshamilton directedevolutionlibrarycreationmethodsandprotocols AT georgiougeorge directedevolutionlibrarycreationmethodsandprotocols |