Proteoglycan protocols
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| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in molecular biology
171 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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MARC
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| 245 | 1 | 0 | |a Proteoglycan protocols |c ed. by Renato V. Iozzo |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a XVI, 558 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 171 | |
| 650 | 4 | |a Protéoglycanes - Manuels de laboratoire | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Proteoglycans |v Laboratory manuals | |
| 650 | 4 | |a Proteoglycans |x analysis |v Laboratory Manuals | |
| 650 | 4 | |a Proteoglycans |x chemistry |v Laboratory Manuals | |
| 650 | 4 | |a Proteoglycans |x isolation & purification |v Laboratory Manuals | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009451149 | |
Datensatz im Suchindex
| DE-BY-UBR_call_number | 00/WC 4150 W181 |
|---|---|
| DE-BY-UBR_katkey | 3182743 |
| DE-BY-UBR_location | 00 |
| DE-BY-UBR_media_number | 069030972770 |
| _version_ | 1822685406028627968 |
| adam_text | Contents
Preface v
Contributors xi
I Isolation and Purification
1 Isolation of Proteoglycans from Cell Cultures and Tissues
Masaki Yanagishita 3
2 Isolation of Proteoglycans from Tendon
Kathryn G. Vogel and Julie A. Peters 9
3 Purification of Proteoglycans from Mineralized Tissues
Neal S. Fedarko 19
4 Purification of Perlecan from Endothelial Cells
John Whitelock 27
5 Isolation and Characterization of Nervous Tissue Proteoglycans
Yu Yamaguchi 35
6 Analysis of Proteoglycans and Glycosaminoglycans
from Drosophila
William D. Staatz, Hidenao Toyoda, Akiko Kinoshita Toyoda,
Kimberlly Chhor, and Scott B. Selleck 41
7 Cartilage and Smooth Muscle Cell Proteoglycans
Detected by Affinity Blotting Using Biotinylated Hyaluronan
James Melrose 53
8 Preparation of Proteoglycans for N Terminal and Internal
Amino Acid Sequence Analysis
Peter J. Neame 67
9 High Specific Activity 35S Labeled Heparan Sulfate
Prepared from Cultured Cells
Nicholas W. Shworak 79
10 Selective Detection of Sulfotransferase Isoforms
by the Ligand Affinity Conversion Approach
Nicholas W. Shworak 91
11 Paniculate and Soluble Glycosaminoglycan Synthesizing
Enzymes: Preparation Assay and Use
Geetha Sugumaran and Jeremiah E. Silbert 103
vii
I//// Contents
12 Disaccharide Composition of Hyaluronan
and Chondroitin/Dermatan Sulfate:
Analysis with Fluorophore Assisted
Carbohydrate Electrophoresis
Anna H. K. Plaas, L. West, Ronald J. Midura,
and Vincent C. Hascall 117
13 Integral Glycan Sequencing of Heparan Sulfate
and Heparin Saccharides
Jeremy E. Turnbull 129
14 Analytical and Preparative Strong Anion Exchange HPLC
of Heparan Sulfate and Heparin Saccharides
Jeremy E. Turnbull 141
15 Proteoglycans Analyzed by Composite Gel Electrophoresis
and Immunoblotting
George R. Dodge and Ralph Heimer 149
16 Quantitation of Proteoglycans in Biological Fluids
Using Alcian Blue
Madeleine Karlsson and Sven Bjornsson 159
17 Cellulose Acetate Electrophoresis of Glycosaminoglycans
Yanusz Wegrowski and Francois Xavier Maquart 175
18 Disaccharide Composition in Glycosaminoglycans/Proteoglycans
Analyzed by Capillary Zone Electrophoresis
Nikos K. Karamanos and Anders Hjerpe 181
19 Intact and Oligomeric Glycosaminoglycans
Analyzed with Capillary Electrophoresis
Nikos K. Karamanos and Anders Hjerpe 193
II Expression, Detection, and Degradation
20 Recombinant Expression of Proteoglycans in Mammalian Cells:
Utility and Advantages of the Vaccinia Virus/T7 Bacteriophage
Hybrid Expression System
David J. McQuillan, Neung Seon Seo, Anne M. Hocking,
and Camille I. McQuillan 201
21 Purification of Recombinant Human Decorin and Its Subdomains
Anna L. McBain and David M. Mann 221
22 Prokaryotic Expression of Proteoglycans
Alan D. Murdoch and Renato V. lozzo 231
23 Proteoglycan Gene Targeting in Somatic Cells
Giancarlo Ghiselli and Renato V. lozzo 239
Contents ix
24 Constitutive and Inducible Antisense Expression
Melissa Handler and Renato V. lozzo 251
25 Cell Mediated Transfer of Proteoglycan Genes
Jens W. Fischer, Michael G. Kinsella, David Hasenstab,
Alexander W. Clowes, and Thomas N. Wight 261
26 Morphological Evaluation of Proteoglycans in Cells and Tissues
Stephanie L. Lara, Stephen P. Evanko, and Thomas N. Wight... 271
27 Expression and Characterization of Engineered Proteoglycans
Wei Luo, Chunxia Guo, Jing Zheng, and Marvin L. Tamer 291
28 Intracellular Localization of Engineered Proteoglycans
Tung Ling L. Chen, Peiyin Wang, Seung S. Gwon,
Nina W. Flay, and Barbara M. Vertel 301
29 Selection of Glycosaminoglycan Deficient Mutants
Xiaomei Bai, Brett Crawford, and Jeffrey D. Esko 309
30 Xyloside Priming of Glycosaminoglycan Biosynthesis
and Inhibition of Proteoglycan Assembly
Timothy A. Fritz and Jeffrey D. Esko 377
31 Inhibition of Heparan Sulfate Synthesis by Chlorate
H. Edward Conrad 325
32 Detection of Proteoglycan Core Proteins
with Glycosaminoglycan Lyases and Antibodies
John R. Couchman and Pairath Tapanadechopone 329
33 Proteoglycan Core Proteins and Catabolic Fragments
Present in Tissues and Fluids
John D. Sandy 335
34 Degradation of Heparan Sulfate by Nitrous Acid
H. Edward Conrad 347
35 Degradation of Heparan Sulfate with Heparin Lyases
Laurie A. LeBrun and Robert J. Linhardt 353
36 Degradation of Chondroitin Sulfate and Dermatan Sulfate
with Chondroitin Lyases
Maria Jose Hernaiz and Robert J. Linhardt 363
37 In Vitro Assays for Hyaluronan Synthase
Andrew P. Spicer 373
38 Hyaluronidase Activity and Hyaluronidase Inhibitors:
Assay Using a Microtiter Based System
Susan Stair Nawy, Antonei B. Csoka, Kazuhiro Mio,
and Robert Stern 383
x Contents
39 Detecting Hyaluronidase and Hyaluronidase Inhibitors:
Hyaluronan Substrate Gel and Inverse Substrate Gel Techniques
Kazuhiro Mio, Antonei B. Csoka, Susan Stair Nawy,
and Robert Stern 391
III Interactions
40 Affinity Coelectrophoresis of Proteoglycan Protein Complexes
James D. San Antonio and Arthur D. Lander 401
41 Binding Constant Measurements for Inhibitors
of Growth Factor Binding to Heparan Sulfate Proteoglycans
Kimberly E. Forsten and Matthew A. Nugent 415
42 Interaction of Proteoglycans with Receptor Tyrosine Kinases
David K. Moscatello and Renato V. lozzo 427
43 Measuring Single Cell Cytosolic Ca2+ Concentration
in Response to Proteoglycans
Sandip Patel and Renato V. lozzo 435
44 Serum Amyloid A Peptide Interactions with Glycosaminoglycans:
Evaluation by Affinity Chromatography
John B. Ancsin and Robert Kisilevsky 449
45 Interactions of Lipoproteins with Proteoglycans
Kevin Jon Williams 457
46 Hyaluronan and Hyaluronan Binding Proteins:
Probes for Specific Detection
Bryan P. Toole, Qin Yu, and Charles B. Underhill 479
47 Novel Confocal FRAP Analysis of Carbohydrate Protein
Interactions Within the Extracellular Matrix
Philip Gribbon, Boon C. Heng, and Timothy E. Hardingham 487
48 Regulatory Roles of Syndecans in Cell Adhesion and Invasion
J. Kevin Langford and Ralph D. Sanderson 495
49 Optical Biosensor Techniques to Analyze
Protein Polysaccharide Interactions
David G. Fernig 505
50 Phage Display Technology to Obtain Antiheparan Sulfate Antibodies
T. H. van Kuppevelt, G. J. Jenniskens, J. H. Veerkamp,
G. B. ten Dam, and M. A. B. A. Dennissen 519
51 Tissue Specific Binding by FGF and FGF Receptors
to Endogenous Heparan Sulfates
Andreas Fried!, Mark Filla, and Alan C. Rapraeger 535
Index 547
|
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| dewey-search | 572/.68 |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content Protoglykane gnd |
| genre_facet | Aufsatzsammlung Protoglykane |
| id | DE-604.BV013820568 |
| illustrated | Illustrated |
| indexdate | 2024-12-23T15:38:27Z |
| institution | BVB |
| isbn | 0896037592 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009451149 |
| oclc_num | 44712817 |
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| owner_facet | DE-355 DE-BY-UBR DE-12 |
| physical | XVI, 558 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | Proteoglycan protocols Methods in molecular biology Protéoglycanes - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Proteoglycans Laboratory manuals Proteoglycans analysis Laboratory Manuals Proteoglycans chemistry Laboratory Manuals Proteoglycans isolation & purification Laboratory Manuals Proteoglykane (DE-588)4176001-3 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4176001-3 (DE-588)4038971-6 (DE-588)4039983-7 (DE-588)4143413-4 |
| title | Proteoglycan protocols |
| title_auth | Proteoglycan protocols |
| title_exact_search | Proteoglycan protocols |
| title_full | Proteoglycan protocols ed. by Renato V. Iozzo |
| title_fullStr | Proteoglycan protocols ed. by Renato V. Iozzo |
| title_full_unstemmed | Proteoglycan protocols ed. by Renato V. Iozzo |
| title_short | Proteoglycan protocols |
| title_sort | proteoglycan protocols |
| topic | Protéoglycanes - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Proteoglycans Laboratory manuals Proteoglycans analysis Laboratory Manuals Proteoglycans chemistry Laboratory Manuals Proteoglycans isolation & purification Laboratory Manuals Proteoglykane (DE-588)4176001-3 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Protéoglycanes - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten Proteoglycans Laboratory manuals Proteoglycans analysis Laboratory Manuals Proteoglycans chemistry Laboratory Manuals Proteoglycans isolation & purification Laboratory Manuals Proteoglykane Methode Molekularbiologie Aufsatzsammlung Protoglykane |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009451149&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
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