Enhanced utilisation of an infectious plant viral cDNA clone
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| Format: | Buch |
| Sprache: | English |
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Uppsala
Swedish Univ. of Agricultural Sciences
2001
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| Schriftenreihe: | Sveriges Lantbruksuniversitet <Uppsala u.a.>: [Acta Universitatis Agriculturae Sueciae / Agraria]
260 |
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 1001/10.1997 A 261-260 |
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| adam_text | Titel: Enhanced utilisation of an infectious plant viral cDNA clone
Autor: Kekarainen, Tuija
Jahr: 2001
Contents
Introduction 11
Potyviruses: economically important plant RNA viruses 12
Genome structure and organisation 12
Transmission, and transport in planta 14
Non-translated regions of the potyvirus genome 14
Replication and translation: genome amplification 14
Studying the potyvirus genome 16
Infectious cDNA: an essential tool to study potyviruses 16
Deletion mutagenesis 17
Site-directed mutagenesis 17
Alanine scanning 17
Chimeric constructs 18
Insertion mutagenesis 18
Inoculation of plants by mutant icDNA 18
Transposons in functional genetics 19
Transposons in in vitro mutagenesis 19
Bacteriophage Mu in vitro transposition and its applications 20
Aims of the study 21
Results and discussion 22
Genetic and biological variability among PVA isolates (I) 22
Efficient inoculation of PVA cDNA to plants (II) 23
Mu in vitro transposition mutagenesis of PVA (HI) 24
Constructing the insertion mutant library of PVA 25
Selection of PVA mutants in protoplasts 25
Method to analyse insertion library: genetic footprinting 25
Functional characterisation of PVA genome 26
Conclusions 27
Future perspectives 27
References 28
Acknowledgements 37
|
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| discipline | Biologie Agrarwissenschaft Pflanzenbau |
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| id | DE-604.BV013632667 |
| illustrated | Illustrated |
| indexdate | 2024-11-25T17:19:56Z |
| institution | BVB |
| isbn | 9157657815 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009313635 |
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| physical | Getr. Zählung Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Swedish Univ. of Agricultural Sciences |
| record_format | marc |
| series2 | Sveriges Lantbruksuniversitet <Uppsala u.a.>: [Acta Universitatis Agriculturae Sueciae / Agraria] |
| spellingShingle | Kekarainen, Tuija Enhanced utilisation of an infectious plant viral cDNA clone Plant viruses Potyviruses Potyviruses Genetics Kartoffel-A-Virus (DE-588)4648545-4 gnd Genanalyse (DE-588)4200230-8 gnd Insertionsmutagenese (DE-588)4212813-4 gnd |
| subject_GND | (DE-588)4648545-4 (DE-588)4200230-8 (DE-588)4212813-4 (DE-588)4113937-9 |
| title | Enhanced utilisation of an infectious plant viral cDNA clone |
| title_auth | Enhanced utilisation of an infectious plant viral cDNA clone |
| title_exact_search | Enhanced utilisation of an infectious plant viral cDNA clone |
| title_full | Enhanced utilisation of an infectious plant viral cDNA clone Tuija Kekarainen |
| title_fullStr | Enhanced utilisation of an infectious plant viral cDNA clone Tuija Kekarainen |
| title_full_unstemmed | Enhanced utilisation of an infectious plant viral cDNA clone Tuija Kekarainen |
| title_short | Enhanced utilisation of an infectious plant viral cDNA clone |
| title_sort | enhanced utilisation of an infectious plant viral cdna clone |
| topic | Plant viruses Potyviruses Potyviruses Genetics Kartoffel-A-Virus (DE-588)4648545-4 gnd Genanalyse (DE-588)4200230-8 gnd Insertionsmutagenese (DE-588)4212813-4 gnd |
| topic_facet | Plant viruses Potyviruses Potyviruses Genetics Kartoffel-A-Virus Genanalyse Insertionsmutagenese Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009313635&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV010948960 |
| work_keys_str_mv | AT kekarainentuija enhancedutilisationofaninfectiousplantviralcdnaclone |