Extracellular matrix protocols
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| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2000
|
| Schriftenreihe: | Methods in molecular biology
139 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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Datensatz im Suchindex
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| _version_ | 1822711349023604737 |
| adam_text | Contents
Preface v
Contributors xi
Part I. Biochemistry of Extracellular Matrix
1 Semipermeabilized Cells to Study Procollagen Assembly
Richard R. Wilson and Neil J. Bulleid 1
2 Quantitative Determination of Collagen Crosslinks
Trevor J. Sims, Nicholas C. Avery, and Allen J. Bailey 11
3 Analysis of Laminin Structure and Function with Recombinant
Glycoprotein Expressed in Insect Cells
ToddL. Mathus and Peter D. Yurchenco 27
4 Recombinant Collagen Trimers from Insect Cells and Yeast
Johanna Myllyharju 39
5 Eukaryotic Expression and Purification of Recombinant
Extracellular Matrix Proteins Carrying the Strep II Tag
Neil Smyth, Uwe Odenthal, Barbara Merkl,
and Mats Paulsson 49
Part II. Biophysics of Extracellular Matrix Proteins
6 Preparation of Isotopically Labeled Recombinant Fragments
of Fibronectin for Functional and Structural Study
by Heteronuclear Nuclear Magnetic Resonance Spectroscopy
Jeremy R. Bright, Andrew R. Pickford, Jennifer R. Potts,
and lain D. Campbell 59
7 Reconstitution of Functional Integrin into Phopsholipid Vesicles
and Planar Lipid Bilayers
Eva Maria Erb and Jurgen Engel 71
8 Confocal FRAP Analysis of ECM Molecular Interactions
Timothy Hardingham and Philip Gribbon 83
9 Electron Cryomicroscopy of Fibrillar Collagens
Roger S. Meadows, David F. Holmes, Chris J. Gilpin,
and Karl E. Kadler 95
10 Atomic Force Microspopy Measurements of Intermolecular
Binding Strength
Gradimir N. Misevic 111
vii
V
viii Contents
11 ECM Macromolecules: Rotary Shadowing and Scanning
Transmission Electron Microscopy r
Michael J. Sherratt, Helen K. Graham, Cay M. Kielty,
and David F. Holmes 119
Part III. Molecular Biology of Extracellular Matrix Genes i
t
12 Screening for Mutations in Cartilage ECM Genes
Michael D. Briggs 133
13 Tissue Specific KO of ECM Proteins
Emilio Hirsch, Mara Brancaccio, and Fiorella Altruda 147 r
14 Homologous Gene Targeting to Study ECM Assembly
Francesco Ramirez, Friedrich Laub,
and Hideaki Sumiyoshi 179
15 Enhancer Analysis of the a 1(11) and a2(XI) Collagen Genes
in Transfected Chondrocytes and Transgenic Mice
Noriyuki Tsumaki, Ying Liu, Yoshihiko Yamada,
and Paul Krebsbach 187
16 Retroviral Delivery of ECM Genes to Cells
Kyonggeun Yoon and Vitali Alexeev 197
Part IV. Cell Biology of the Extracellular Matrix
17 Using Self Assembled Monolayers to Pattern ECM Proteins
and Cells on Subtrates
Christopher S. Chen, Emanuele Ostuni, George M. Whitesides,
and Donald E. Ingber 209
18 Methods for Preparing Extracellular Matrix and Quantifying
Insulin like Growth Factor Binding Protein Binding to the ECM
Bo Zheng and David Ft. Clemmons 221
19 Measuring Interactions Between ECM and TGFp Like Proteins
Sarah L Dallas 231
20 Using Organotypic Tissue Slices as Substrata for the Culture
of Dissociated Cells
Daniel E. Emerling and Arthur D. Lander 245
21 Neuroepithelial Differentiation Induced by ECM Molecules
Jose Maria Frade and Alfred Rodriguez T bar 257
22 Migration Assays for Oligodendrocyte Precursor Cells
Emma E. Frost, Richard Milner,
and Charles ffrench Constant 265
23 Cell Adhesion Assays
Martin J. Humphries 279
Contents ix
24 Tissue Engineering and Cell Populated Collagen Matrices
Paul D. Kemp 287
25 Solid Phase Assays for Studying ECM Protein Protein Interactions
A. Paul Mould 295
26 Tissue Engineering of Cartilage
Ronda E. Schreiber and Anthony Ratcliffe 301
27 Tissue Recombinants to Study Extracellular Matrix
Targeting to Basement Membranes
Patricia Simon Assmann and Michele Kedinger 311
28 Fluorescence Assays to Study Cell Adhesion
and Migration In Vitro
Paola Spessotto, Emiliana Giacomello, and Roberto Penis 321
29 Analyzing Cell ECM Interactions in Adult Mammary Gland
by Transplantation of Embryonic Mammary Tissue
from Knockout Mice
Teresa C. M. Klinowska and Charles H. Streuli 345
Index 359
|
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| discipline | Biologie |
| format | Book |
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| genre_facet | Aufsatzsammlung Aufsatzsammlung - Biophysik - Methode - Grundsubstanz Aufsatzsammlung - Grundsubstanz - Biochemische Methode Aufsatzsammlung - Methode - Cytologie - Grundsubstanz Aufsatzsammlung - Methode - Molekularbiologie - Grundsubstanz |
| id | DE-604.BV013224019 |
| illustrated | Illustrated |
| indexdate | 2024-12-23T15:23:53Z |
| institution | BVB |
| isbn | 0896036243 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009011441 |
| oclc_num | 247525097 |
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| physical | XIV, 370 S. Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spellingShingle | Extracellular matrix protocols Methods in molecular biology Grundsubstanz - Biochemische Analyse Proteine - Grundsubstanz - Biochemische Analyse Extracellular matrix proteins Laboratory manuals Extracellular matrix Laboratory manuals Cytologie (DE-588)4070177-3 gnd Methode (DE-588)4038971-6 gnd Biochemische Analyse (DE-588)4255721-5 gnd Proteine (DE-588)4076388-2 gnd Biochemische Methode (DE-588)4319880-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Biophysik (DE-588)4006891-2 gnd Grundsubstanz (DE-588)4224942-9 gnd |
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| title | Extracellular matrix protocols |
| title_auth | Extracellular matrix protocols |
| title_exact_search | Extracellular matrix protocols |
| title_full | Extracellular matrix protocols ed. by Charles H. Streuli ... |
| title_fullStr | Extracellular matrix protocols ed. by Charles H. Streuli ... |
| title_full_unstemmed | Extracellular matrix protocols ed. by Charles H. Streuli ... |
| title_short | Extracellular matrix protocols |
| title_sort | extracellular matrix protocols |
| topic | Grundsubstanz - Biochemische Analyse Proteine - Grundsubstanz - Biochemische Analyse Extracellular matrix proteins Laboratory manuals Extracellular matrix Laboratory manuals Cytologie (DE-588)4070177-3 gnd Methode (DE-588)4038971-6 gnd Biochemische Analyse (DE-588)4255721-5 gnd Proteine (DE-588)4076388-2 gnd Biochemische Methode (DE-588)4319880-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Biophysik (DE-588)4006891-2 gnd Grundsubstanz (DE-588)4224942-9 gnd |
| topic_facet | Grundsubstanz - Biochemische Analyse Proteine - Grundsubstanz - Biochemische Analyse Extracellular matrix proteins Laboratory manuals Extracellular matrix Laboratory manuals Cytologie Methode Biochemische Analyse Proteine Biochemische Methode Molekularbiologie Biophysik Grundsubstanz Aufsatzsammlung Aufsatzsammlung - Biophysik - Methode - Grundsubstanz Aufsatzsammlung - Grundsubstanz - Biochemische Methode Aufsatzsammlung - Methode - Cytologie - Grundsubstanz Aufsatzsammlung - Methode - Molekularbiologie - Grundsubstanz |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009011441&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT streulicharlesh extracellularmatrixprotocols |