Plant conservation biotechnology

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Veröffentlicht: London [u.a.] Taylor & Francis 1999
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Datensatz im Suchindex

DE-BY-TUM_call_number 1001 10.1999 B 758
DE-BY-TUM_katkey 1106958
DE-BY-TUM_location Mag
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adam_text Plant Conservation Biotechnology Edited by ERICA E BENSON University of Abertay, Dundee UK Contents List of Figures page xiii Foreword xv Preface xvii Acknowledgements xix Contributing Authors xxi PART ONE Principles of Plant Conservation Biotechnology: Methods, Techniques and Procedures 1 An Introduction to Plant Conservation Biotechnology 3 Erica E Benson 1 1 Integrating biotechnology into conservation programmes 3 12A general overview: how does biotechnology assist plant conservation? 4 1 3 Conservation biotechnology and the sustainable utilization of plant genetic resources 7 1 4 Conclusions and future prospects 8 References 9 Molecular Approaches to Assessing Plant Diversity 11 Stephen A Harris 2 1 Introduction 11 2 2 Molecular marker systems 12 2 3 Molecular markers in germplasm characterization 16 2 4 Molecular markers in systematics and population genetics 17 2 5 Prospects for molecular markers in biodiversity characterization 18 References 19 Biotechnology in Plant Germplasm Acquisition 25 Kim E Hummer 3 1 Introduction 25 3 2 Plant genetic resource conservation 25 vi Contents 321 In situ conservation 26 322 Ex situ conservation 26 323 Static and dynamic conservation 27 3 3 Acquisition procedures 27 331 Plant exploration 27 332 Plant exchange 28 3 4 Acquisition planning 29 341 Intellectual property rights 30 342 Quarantine regulations 31 3 5 Methods of acquisition 32 351 Seeds 32 352 Vegetative propagation 32 3 6 Documentation 34 3 7 Identity confirmation 34 3 8 Summary 36 References 36 4 Tissue Culture Techniques in In Vitro Plant Conservation 41 Paul T Lynch 4 1 Introduction 41 4 2 In vitro propagation 41 421 Germplasm acquisition 43 422 Selection of tissue for in vitro culture 43 423 Microbial contamination and disease indexing 44 424 Tissue culture media 45 425 Problems of culture establishment 48 426 Propagule multiplication (morphogenesis) 49 427 Plantlet development 50 4 3 Acclimatization of in vitro germplasm to in vivo conditions 50 4 4 In vitro culture recalcitrance 52 4 5 Embryo rescue 52 4 6 Use of plant tissue culture for germplasm storage 53 461 Slow (minimal) growth 53 462 Recovery of germplasm after storage 53 4 7 Facilities for plant tissue culture 54 4 8 Conclusions 54 References 55 5 Phytosanitary Aspects of Plant Germplasm Conservation 63 Robert R Martin and Joseph D Postman 5 1 Introduction 63 5 2 Safe movement of germplasm 63 521 Quarantine 64 522 International cooperation 64 5 3 International guidelines 67 5 4 Virus detection 68 541 Requirements for detection and diagnosis 69 542 Serological detection 70 543 Nucleic acid based assays 71 Contents vii 544 Detection based on more traditional methods 72 545 The significance of a test result 73 5 5 Production of pathogen-free plants 73 551 Heat therapy 74 552 Meristem tip culture 75 553 Chemotherapy 76 5 6 Conclusions 78 References 78 6 Cryopreservation 83 Erica E Benson 6 1 Introduction 83 6 2 Principles of cryopreservation and germplasm preparation 83 621 Preparing germplasm for cryopreservation 84 622 Pre-treatments 85 6 3 Cryprotection 85 631 Traditional cryoprotection and controlled rate cooling 86 632 Vitrification 88 6 4 Freezing and long-term cryogenic storage 89 641 Controlled rate freezing 89 642 Rapid freezing and long-term storage 90 6 5 Post-cryopreservation recovery 90 6 6 Cryopreservation protocols: techniques and practical considerations 91 6 7 Conclusions 93 References 93 7 Stability Assessments of Conserved Plant Germplasm 97 Keith Harding 7 1 Introduction 97 7 2 Natural variation in populations 97 7 3 Techniques to assess genetic stability 98 7 4 Morphological variation 98 7 5 Cytological analysis 99 7 6 Biochemical analysis 99 7 7 Molecular analysis 99 771 Genome structure 100 772 Techniques 100 7 8 Conclusion 103 References 103 PART TWO Applications of Biotechnology in Plant Diversity Conservation 109 8 Conservation Strategies for Algae 111 John G Day 8 1 Introduction 111 8 2 Alternative strategies employed to conserve algae 112 8 3 Roles of genetic resource centres and culture collections 113 8 4 Methodological strategies employed to conserve algae 113 viii Contents 841 Maintenance by serial subculture 113 842 Maintenance by storage in liquid medium 115 843 Drying techniques 115 844 Cryopreservation Techniques 116 8 5 Concluding comments 120 Acknowledgements 121 References 121 9 Cryo-conservation of Industrially Important Plant Cell Cultures 125 Heinz Martin Schumacher 9 1 Introduction: the biotechnological use of dedifferentiated plant cell cultures 125 9 2 Stability of product formation after cryopreservation 126 921 Anthocyanins 126 922 Ginsenosides 127 923 Diosgenine 128 924 Rosmarinic acid 128 925 Biotin 129 926 Indole alkaloids 129 927 Berberine 131 9 3 Stability of a biosynthetic capacity: cardiac glycosides 131 9 4 Transformed root cultures for secondary metabolite production 132 9 5 Conclusions 133 References 136 10 In Vitro Conservation of Temperate Tree Fruit and Nut Crops 139 Barbara M Reed 10 1 Introduction 139 10 2 Literature review of progress 140 10 2 1 Medium-term storage at above freezing temperatures 140 10 2 2 Long-term storage in liquid nitrogen 142 10 3 Germplasm storage 145 10 3 1 In vitro stored collections 145 10 3 2 Cryopreserved collections 145 10 3 3 Discussion: the role of storage technologies 146 10 4 Impact on the storage and distribution of germplasm 148 10 4 1 In vitro storage 148 10 4 2 Cryopreservation 148 10 5 Conclusions 149 References 149 11 Conservation of Small Fruit Germplasm 155 Rex M Brennan and Stephen Millam 11 1 Introduction 155 11 1 1 Small fruit germplasm collections 155 11 2 Small fruit breeding 156 11 3 Problems related to field-based collections 156 11 4 World-wide small fruit germplasm collections 157 11 5 In vitro conservation and cryopreservation 158 Contents ix 11 5 1 Tissue culture 158 11 5 2 Cold storage of tissue cultures 159 11 5 3 Cryopreservation 159 11 6 The use of pollen storage for small fruit germplasm conservation 160 11 7 Conservation of transgenic plant small fruit plant germplasm 161 11 8 Genetic stability aspects 161 11 9 DNA banking 162 11 10 Conclusions 162 Acknowledgements 162 References 162 12 Biotechnological Advances in the Conservation of Root and Tuber Crops 165 Ali M Colmirzaie, Ana Panta and Judith Toledo 12 1 Introduction 165 12 2 Establishment of aseptic cultures 166 12 2 1 Infection produced by viroids 167 12 2 2 Infection produced by viruses 167 12 2 3 Infection produced by systemic bacteria 167 12 3 In vitro maintenance 168 12 3 1 Short-term storage 168 12 3 2 Long-term storage 168 12 3 3 In vitro tuberization for long-term conservation 170 12 4 Other operational considerations for germplasm maintenance 170 12 5 Advances in germplasm utilization 171 12 6 Cryopreservation 172 12 6 1 Potato cryopreservation 172 12 6 2 Sweet potato cryopreservation 175 12 7 Genetic stability 175 12 8 Conclusions 175 References 176 13 Biotechnology in Germplasm Management of Cassava and Yams 179 SYC Ng, S H Mantel! and N Q Ng 13 1 Introduction 179 13 1 1 Origin, distribution and cultivation 179 13 1 2 Genetic diversity 180 13 2 Integrated strategy for germplasm conservation 181 13 2 1 In situ conservation 181 13 2 2 Ex situ conservation 182 13 3 Tissue culture methods for germplasm conservation and exchange 183 13 3 1 Meristem/shoot-tip culture 183 13 3 2 Axillary bud and nodal cultures 184 13 3 3 Embryo culture 184 13 3 4 Microtuberization 185 13 3 5 Adventitive regeneration 185 13 3 6 Reduced growth storage 186 13 3 7 Cryopreservation 187 x Contents 13 4 Biochemical and molecular fingerprinting for auditing of germplasm collection 187 13 4 1 Range of methods available 187 13 4 2 Isozyme fingerprinting 188 13 4 3 Use of RAPD-PCR, microsatellites and other molecular markers 190 13 5 Applications to germplasm conservation, exchange, and improvement 192 13 5 1 Germplasm exchange 192 13 5 2 Germplasm conservation 197 13 5 3 Conservation biotechnology and germplasm improvement 199 13 6 Conclusions and future prospects 201 References 202 14 Conservation Biotechnology of Endemic and other Economically Important Plant Species of India 211 Binay B Mandal 14 1 Introduction 211 14 2 In vitro techniques in conservation 212 14 3 Propagation 212 14 3 1 Somatic embryogenesis 214 14 3 2 Production of storage organs in vitro 214 14 4 Medium-term conservation 214 14 5 Conservation at normal culture room temperature (25°C) 217 14 5 1 Conservation under normal growing conditions 217 14 5 2 Conservation with media manipulation 217 14 5 3 Conservation using induced storage organs 217 14 6 Long-term conservation using cryopreservation 218 14 6 1 Cryopreservation of recalcitrant seed species 219 14 6 2 Cryopreservation of clonally propagated species 219 14 7 Characterization, classification and monitoring 220 14 7 1 Characterization and classification of germplasm 220 14 7 2 Monitoring genetic stability of conserved germplasm 221 14 8 In vitro conservation activities at other research stations in India 222 14 9 Conclusions 223 References 223 15 The Application of Biotechnology for the Conservation of Endangered Plants 227 Valerie C Pence 15 1 Introduction 227 15 2 Special aspects of endangered species work 227 15 3 In vitro propagation technologies and endangered plant species 228 15 3 1 In vitro propagation using seed 228 15 3 2 Propagation without seeds 235 15 4 Genetic diversity and genetic stability 236 15 5 Uses of in vitro propagated plants of endangered species 236 15 6 Application of preservation technologies 237 15 7 Application of in vitro collection biotechnology 239 Contents xi 15 8 Application of genetic analysis and molecular techniques to endangered plant species conservation 240 15 9 Summary 241 References 241 16 Conservation of the Rare and Endangered Plants Endemic to Spain 251 M E Gonzalez-Benito, C Martin, J M Iriondo and C Perez 16 1 Introduction 251 16 2 The application of micropropagation and in vitro conservation to endangered plants endemic to Spain 252 16 3 Cryopreservation and the conservation of endangered endemic Spanish plants 254 16 3 1 Cryopreservation of orthodox seeds 254 16 3 2 Cryopreservation of vegetatively propagated germplasm 257 16 4 Stability assessments 257 16 5 Plant diversity assessment for endangered species conservation 259 16 6 Conclusions 260 Acknowledgements 261 References 262 17 Recalcitrant Seed Biotechnology Applications to Rain Forest Conservation 265 M Marzalina and B Krishnapillay 17 1 Introduction 265 17 2 Seed characteristics 266 17 2 1 Orthodox seeds 266 17 2 2 Recalcitrant seeds 266 17 2 3 Intermediate seeds 267 17 3 Seed collection and handling 267 17 4 Seed storage 267 17 4 1 Short-term and mid-term storage methods 268 17 4 2 Long-term storage 268 17 5 Cryopreservation 269 17 5 1 Cryopreservation of whole seeds 269 17 5 2 Cryopreservation of excised embryos 271 17 5 3 Cryopreservation of shoot tips 273 17 5 4 Cryopreservation of somatic embryos 273 17 6 Conclusions 274 References 274 18 Applications of Biotechnology for the Conservation and Sustainable Exploitation of Plants from Brazilian Rain Forests 277 Ana Maria Viana, Maria Cristina Mazza and Sinclair Mantell 18 1 Introduction 277 18 2 Background 278 18 2 1 The Brazilian forests 278 18 3 Why are Brazilian forests endangered? 280 18 3 1 Abiotic factors 281 18 3 2 Biotic factors 281 xii Contents 18 4 Endangered tree species of the Brazilian forests 281 18 5 Current and potential uses of biotechnology for in situ and ex situ conservation management of Brazilian forest species 283 18 5 1 Defining genetic diversity and differences between populations of flora and fauna 283 18 5 2 Uses of in vitro culture techniques for propagation and conservation 291 18 6 Conclusions and future prospects 294 Acknowledgements 295 References 295 Index 301
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spellingShingle Plant conservation biotechnology
Biotechnologie gtt
Natuurbehoud gtt
Planten gtt
Plantes - Biotechnologie ram
Plantes - Conservation ram
Plantes - Ressources génétiques ram
Biotechnology methods
Cryopreservation
Germplasm resources, Plant
Plant biotechnology
Plants genetics
Genetische Ressourcen (DE-588)4156623-3 gnd
Biotechnologie (DE-588)4069491-4 gnd
Pflanzen (DE-588)4045539-7 gnd
subject_GND (DE-588)4156623-3
(DE-588)4069491-4
(DE-588)4045539-7
title Plant conservation biotechnology
title_auth Plant conservation biotechnology
title_exact_search Plant conservation biotechnology
title_full Plant conservation biotechnology ed. by Erica E. Benson
title_fullStr Plant conservation biotechnology ed. by Erica E. Benson
title_full_unstemmed Plant conservation biotechnology ed. by Erica E. Benson
title_short Plant conservation biotechnology
title_sort plant conservation biotechnology
topic Biotechnologie gtt
Natuurbehoud gtt
Planten gtt
Plantes - Biotechnologie ram
Plantes - Conservation ram
Plantes - Ressources génétiques ram
Biotechnology methods
Cryopreservation
Germplasm resources, Plant
Plant biotechnology
Plants genetics
Genetische Ressourcen (DE-588)4156623-3 gnd
Biotechnologie (DE-588)4069491-4 gnd
Pflanzen (DE-588)4045539-7 gnd
topic_facet Biotechnologie
Natuurbehoud
Planten
Plantes - Biotechnologie
Plantes - Conservation
Plantes - Ressources génétiques
Biotechnology methods
Cryopreservation
Germplasm resources, Plant
Plant biotechnology
Plants genetics
Genetische Ressourcen
Pflanzen
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