Diagnostic cytogenetics
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1999
|
| Schriftenreihe: | Springer lab manual
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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MARC
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| 245 | 1 | 0 | |a Diagnostic cytogenetics |c Rolf-Dieter Wegner (ed.) |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1999 | |
| 300 | |a XIV, 460 S. |b Ill., graph Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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Datensatz im Suchindex
| _version_ | 1819646879326011392 |
|---|---|
| adam_text | Part I Classical Cytogenetics
Chapter 1
Tissue Culture
FRIEDEL WENZEL 3
Subprotocol 1: Sampling, Transport, Storage 20
Subprotocol 2: Ciyopreservation 26
Subprotocol 3: Bacterial and/or fungal contamination 33
Subprotocol 4: Mycoplasma Contamination 37
Subprotocol 5: Elimination of Mycoplasmas from Cell Cultures .... 45
Chapter 2
Chromosome Staining
ANGELIKA KOHLER 52
Subprotocol 1: Giemsa Staining 55
Subprotocol 2: GTG Banding 56
Subprotocol 3: QFQ Banding 59
Subprotocol 4: C Banding 60
Subprotocol 5: NOR Staining 62
Subprotocol 6: DA DAPI Staining 64
Subprotocol 7: Replication Pattern (by BrdU Incorporation) 65
Subprotocol 8: Sister Chromatid Differentiation
(by BrdU Incorporation) 68
Subprotocol 9: Sex Chromatin Staining X Chromatin (Barr Body) . 70
Subprotocol 10: Sex Chromatin Staining Y Chromatin (Y Body) . . 72
Chapter 3
Karyotyping and Data Interpretation
KARSTEN HELD 75
Chapter 4
Documentation
PETER MINY AND ROLF DIETER WEGNER 96
Part II Postnatal Diagnosis
Chapter 5
Peripheral Blood
IRIS BARTELS 115
Chapter 6
Establishment of Permanent Growing Lymphoblastoid Cell Lines
HEIDEMARIE NEITZEL 121
Subprotocol 1: Transformation of B Lymphocytes
with B95 8 EB Virus 123
Subprotocol 2: Ficoll Separation of Unfractionated Mononuclear
Leukocytes Obtained from Whole Blood 125
Subprotocol 3: Establishment of Cultures 126
Subprotocol 4: Freezing of Lymphoblastoid Cells 127
Subprotocol 5: Thawing of Lymphoblastoid Cells 128
Subprotocol 6: Chromosome Preparations
from Lymphoblastoid Cells 129
Chapter 7
Solid Tissues
REGINE SCHUBERT AND GESA SCHWANITZ 132
Chapter 8
Cells from Urine Sample
HANNELORE KORNER, HENRIKE DIA AND CHRISTIANE BOMMER ... 142
Chapter 9
Classical and Molecular Cytogenetics of Tumor Cells
BRIGITTE SCHLEGELBERGER, SIMONE METZKE, SVETLANA HARDER,
REINA ZUHLKE JENISCH.YANMING ZHANG, REINER SIEBERT 151
Subprotocol 1: Chromosome Analysis 152
Subprotocol 2: Fluorescence in Situ Hybridization 168
Chapter 10
Cytogenetics of Meiotic Cells
R. JOHANNISSON 186
Subprotocol 1: Air Drying Method 188
Subprotocol 2: Surface Spreading Method Using Light Microscopy . . 190
Subprotocol 3: Surface Spreading Using Electron Microscopy 199
Subprotocol 4: Ejaculate 207
Part III Prenatal Diagnosis
Chapter 11
Prenatal Diagnosis An Introduction
ROLF DIETER WEGNER 213
Chapter 12
Amniotic Fluid Cell Analysis
INGO KENNERKNECHT, MAHMOUD DJALALI, GOTTHOLD BARBI,
WALTER JUST AND WALTHER VOGEL 217
Chapter 13
Chorionic Villi Sampling
ROLF DIETER WEGNER AND HOLGER TOENNIES 231
Subprotocol 1: Setting up a Short Term Culture 235
Subprotocol 2: Chromosome Preparation from Short
Term Culture 238
Subprotocol 3: Setting up a Long Term Culture
by Physical Maceration 239
Subprotocol 4: Setting up a Long Term Culture
by Enzymatic Dissociation 241
Subprotocol 5: Cell Harvest and Chromosome Preparation 242
Part IV Special Applications
Chapter 14
Diagnosis of Chromosomal Instability Syndromes
ROLF DIETER WEGNER AND MARKUS STUMM 251
Chapter 15
Flow Cytometric Testing for Syndromes with Chromosomal
Instability, Aplastic Anemia and Related Hematological Disorders
DETLEV SCHINDLER AND HOLGER HOEHN 269
Chapter 16
Cell Fusion
MARKUS STUMM AND ROLF DIETER WEGNER 282
Chapter 17
Origin of Trisomies
GESA SCHWANITZ AND THOMAS EGGERMANN 291
Part V Molecular Cytogenetics
Chapter 18
Fluorescence in Situ Hybridization
GESA SCHWANITZ AND REGINE SCHUBERT 305
Subprotocol 1: Preparation of Slides 310
Subprotocol 2: Labelling of DNA Probes 313
Subprotocol 3: Denaturation and Hybridization 317
Subprotocol 4: Detection of Bio tin Labelled Probes 319
Subprotocol 5: Amplification 322
Subprotocol 6: Simultaneous Two Colour Detection 323
Subprotocol 7: In Situ Hybridization with Unique Sequence Probes . 324
Subprotocol 8: Preparation of Interphase Nuclei 326
Chapter 19
Fluorescence in Situ Hybridization (FISH)
Analysis in Human Sperm Cells
EVELYN KO AND RENEE MARTIN 335
Chapter 20
Microdissection and Reverse Chromosome Painting
GABRIELE SENGER, JORG WEIMER, UWE CLAUSSEN,
AND ILSE CHUDOBA 356
Chapter 21
Comparative genomic hybridisation (CGH)
TRAUDL HENN AND OSKAR A. HAAS 376
Part VI Techniques in Development
Chapter 22
Fetal Cells in Maternal Blood
DOROTHEE GANSHIRT, HENK S.P. GARRITSEN,
WOLFGANG HOLZGREVE 401
Chapter 23
Spectral Karyotyping in Clinical and Tumor Cytogenetics
EVELIN SCHROCK, YUVAL GARINI, MICHAEL KOHLER
AND THOMAS RIED 416
Chapter 24
Chromosome Analysis by Multiplex FISH (M FISH)
MICHAEL R. SPEICHER 439
Subject Index 457
|
| any_adam_object | 1 |
| author_GND | (DE-588)12087461X |
| building | Verbundindex |
| bvnumber | BV012608316 |
| callnumber-first | R - Medicine |
| callnumber-label | RB155 |
| callnumber-raw | RB155.6 |
| callnumber-search | RB155.6 |
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| ctrlnum | (OCoLC)246604547 (DE-599)BVBBV012608316 |
| dewey-full | 616/.042 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616/.042 |
| dewey-search | 616/.042 |
| dewey-sort | 3616 242 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Book |
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| id | DE-604.BV012608316 |
| illustrated | Illustrated |
| indexdate | 2024-12-23T15:07:20Z |
| institution | BVB |
| isbn | 3540646027 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008563528 |
| oclc_num | 246604547 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29 DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-29 DE-11 |
| physical | XIV, 460 S. Ill., graph Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer lab manual |
| spellingShingle | Diagnostic cytogenetics Cytogenetik - Cytodiagnostik Cytodiagnostik (DE-588)4070174-8 gnd Diagnosesystem (DE-588)4149458-1 gnd Cytogenetik (DE-588)4070176-1 gnd |
| subject_GND | (DE-588)4070174-8 (DE-588)4149458-1 (DE-588)4070176-1 |
| title | Diagnostic cytogenetics |
| title_auth | Diagnostic cytogenetics |
| title_exact_search | Diagnostic cytogenetics |
| title_full | Diagnostic cytogenetics Rolf-Dieter Wegner (ed.) |
| title_fullStr | Diagnostic cytogenetics Rolf-Dieter Wegner (ed.) |
| title_full_unstemmed | Diagnostic cytogenetics Rolf-Dieter Wegner (ed.) |
| title_short | Diagnostic cytogenetics |
| title_sort | diagnostic cytogenetics |
| topic | Cytogenetik - Cytodiagnostik Cytodiagnostik (DE-588)4070174-8 gnd Diagnosesystem (DE-588)4149458-1 gnd Cytogenetik (DE-588)4070176-1 gnd |
| topic_facet | Cytogenetik - Cytodiagnostik Cytodiagnostik Diagnosesystem Cytogenetik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008563528&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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