Microbial physiology and biochemistry laboratory
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| Hauptverfasser: | , |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Oxford Univ. Press
1998
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| Online-Zugang: | Inhaltsverzeichnis |
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| 100 | 1 | |a White, David |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Microbial physiology and biochemistry laboratory |c David White ; George D. Hegeman |
| 264 | 1 | |a New York [u.a.] |b Oxford Univ. Press |c 1998 | |
| 300 | |a XI, 161 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| DE-BY-TUM_call_number | 1001 10.2000 B 1580 |
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| adam_text |
CONTENTS
Introduction vii
Acknowledgments x
Laboratory Rules and Safety xi
Experiment 1. An Exercise in Pipetting and the Beer Lambert Law 1
Experiment 2. Bacterial Growth Curve 6
Experiment 3. Bioassay for Nicotinic Acid 10
Experiment 4. The Effect of Environment on Growth 17
Experiment 5. Lactic Acid Production by Lactic Acid Bacteria 21
Experiment 6. Assay of Protein and RNA in Whole Cells Grown at Different
Growth Rates 27
Experiment 7. Analysis of Diauxic Growth 34
Experiment 8. Assay of Amylase and Protease Secreted by Bacillus subtilis 47
Experiment 9. Concentration of Amylase From Bacillus subtilis by Ammonium
Sulfate Precipitation and Separation From Protease by
Affinity Purification 51
Experiment 10. Ion Exchange Chromatography of Amylase 57
Experiment 11. Induction of Alkaline Phosphatase and the Determination of Its
Cellular Location in Escherichia coli 65
Experiment 12. Assay of Threonine Deaminase: Determination of KM and V^ 72
Experiment 13. Assaying Fructose 1,6 Bisphosphatase in Saccharomyces cerevisiae 82
Experiment 14. Purification of Glucose 6 Phosphate Dehydrogenase 89
Experiment 15. How Escherichia coli Adapts to Anaerobiosis: Nitrate Reductase 110
Experiment 16. Cellular Fatty Acids of Escherichia coli 118
Experiment 17. Chemotaxis of Pseudomonas aeruginosa 124
Experiment 18. Phototaxis of Rhodospirillum centenum 129
Experiment 19. Light Production by Photobacterium phosphoreum 133
Appendix A
Analysis of Experimental Data 138
How To Do Simple Dilutions 140
Guidelines for Writing Laboratory Reports 142
Appendix B
Laboratory Supplies and Equipment 146
Appendix C
Media 147
Buffers 147
Solutions 148
v
Appendix D
RNA Assay Using Orcinol 149
Lowry Protein Assay 149
Bradford Protein Assay 151
Assay for a Amylase Using Starch Conjugated to a Dye 151
Appendix E
The Beer Lambert Law 153
Relative Absorbancies 154
Cell Density versus Klett Units 154
Appendix F
Quantitative Problems 155
Appendix G
Solutions to Problems in Appendix F. 156
Appendix H
Determining the Amount of Inorganic Phosphate that Limits the Growth Yields
of Escherichia coli 158
Factors that Affect the Relative Amounts of Saturated, Unsaturated, and
Branched Chain Fatty Acids 158
Measuring the KM and Vmax of Fructose 1,6 Bisphosphatase and the Inhibition
by AMP. T. 160
Assaying Glycolytic Enzymes and Fructose 1,6 Bisphosphatase in Yeast
Grown on Glucose or Ethanol 160
Regulation of Phosphofructokinase Activity 161
Further Experiments and Demonstrations with Photobacterium 161
vi Contents |
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| id | DE-604.BV011746676 |
| illustrated | Illustrated |
| indexdate | 2025-01-02T13:08:47Z |
| institution | BVB |
| isbn | 0195113136 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007925589 |
| oclc_num | 633958555 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-526 DE-11 |
| owner_facet | DE-M49 DE-BY-TUM DE-526 DE-11 |
| physical | XI, 161 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Oxford Univ. Press |
| record_format | marc |
| spellingShingle | White, David Hegeman, George D. Microbial physiology and biochemistry laboratory Mikrobiologie (DE-588)4316357-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4316357-9 (DE-588)4038971-6 |
| title | Microbial physiology and biochemistry laboratory |
| title_auth | Microbial physiology and biochemistry laboratory |
| title_exact_search | Microbial physiology and biochemistry laboratory |
| title_full | Microbial physiology and biochemistry laboratory David White ; George D. Hegeman |
| title_fullStr | Microbial physiology and biochemistry laboratory David White ; George D. Hegeman |
| title_full_unstemmed | Microbial physiology and biochemistry laboratory David White ; George D. Hegeman |
| title_short | Microbial physiology and biochemistry laboratory |
| title_sort | microbial physiology and biochemistry laboratory |
| topic | Mikrobiologie (DE-588)4316357-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Mikrobiologie Methode |
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| work_keys_str_mv | AT whitedavid microbialphysiologyandbiochemistrylaboratory AT hegemangeorged microbialphysiologyandbiochemistrylaboratory |