Cellular and molecular procedures in developmental biology
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
San Diego [u.a.]
Acad. Press
1998
|
| Schriftenreihe: | Current topics in developmental biology
36 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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Datensatz im Suchindex
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| adam_text | Contents
Contributors xi
Preface xv
1 The Avian Embryo as a Model in Developmental Studies: Chimeras and
in Vitro Clonal Analysis
Elisabeth Dupin, Catherine Ziller, and Nicole M. Le Douarin
I. Introduction 1
II. Quail Chick Embryonic Chimeras 2
III. In Vitro Cloning of Neural Crest Cells 12
IV. Summary 29
References 30
2 Inhibition of Gene Expression by Antisense Oligonucleotides in Chick
Embryos in Vitro and in Vivo
Aixa V. Morales and Flora de Pablo
I. Introduction 37
II. Designing the Antisense Oligodeoxynucleotides 38
III. Controls to Assess the Specificity of Antisense Oligodeoxynucleotide
Effects 40
IV. Application of Oligodeoxynucleotides to Early Chick Embryos 43
V. Optimization of Oligodeoxynucleotide Delivery 45
VI. Concluding Remarks 47
References 48
3 Lineage Analysis Using Retroviral Vectors
Constance L. Cepko, Elizabeth Ryder, Christopher Austin, Jeffrey Golden, and
Shawn Fields Berry
I. Transduction of Genes via Retrovirus Vectors 51
II. Production of Virus Stocks for Lineage Analysis 53
v
vi Contents
III. Replication Competent Helper Virus 54
IV. Determination of Sibling Relationships 56
V. Procedures 59
References 73
4 Use of Dominant Negative Constructs to Modulate Gene Expression
Giorgio Lagna and AH Hemmati Brivanlou
I. Introduction 75
II. Dominant Negative Mutations 77
III. Generation of Dominant Negative Mutants 78
IV. Interpretation of Dominant Negative Effects 82
V. How to Overproduce a Dominant Negative Mutant 88
VI. Examples of Dominant Negative Mutants 88
VII. Concluding Remarks 95
References 96
5 The Use of Embryonic Stem Cells for the Genetic Manipulation
of the Mouse
Miguel Torres
I. Introduction 99
II. Materials and Instrumentation 100
III. Protocols 100
References 114
6 Organoculture of Otic Vesicle and Ganglion
Juan J. Garrido, Thomas Schimmang, Juan Represa, and Fernando Giraldez
I. Introduction 115
II. Organotypic Culture of Otic Vesicles and the Study of Growth Factors and Cell
Proliferation in the Otic Vesicle 116 ;
III. Culture of Auditory and Vestivular Ganglia: Whole Ganglia t
and Dissociated Cells 121 !
IV. Concluding Remarks 126 I
References 129
Contents vii
7 Organoculture of the Chick Embryonic Neuroretina
Enrique J. de la Rosa, Begoha Diaz, and Flora de Pablo
I. Introduction 133
II. Whole Neuroretina Culture 134
III. Evaluation of Cellular Processes in the Whole Neuroretina 137
IV. Evaluation of Cellular Processes in Dissociated Retinal Cells 140
V. Concluding Remarks 142
References 143
8 Embryonic Explant and Slice Preparations for Studies of Cell Migration
and Axon Guidance
Catherine E. Krull and Paul M. Kulesa
I. Introduction 145
II. Preparing and Culturing Chick Embryo Explants 146
III. Chick Embryo Slice Preparation 151
IV. Preparing and Culturing Mouse Embryo Explants 154
V. Evaluation of Explant and Slice Features 156
VI. Explant and Slice Experimental Perturbations 158
VII. Conclusions 158
References 159
9 Culture of Avian Sympathetic Neurons
Alexander v. Hoist and Hermann Roher
I. Introduction 161
II. Dissection of Ganglia 162
III. Dissociation of Ganglia 166
IV. Selection of Neurons or Non neuronal Cells 168
V. Identification of Neuronal and Glial Cell Populations 171
VI. Cell Culture 172
VII. Expression of Exogenous Genes by Transfection 176
VIII. Solutions and Media 178
References 180
viii Contents
10 Analysis of Gene Expression in Cultured Primary Neurons
Ming Ji Fann and Paul H, Patterson
I. Introduction 183
II. Culture of Small Numbers of Sympathetic Neurons in Chemically Defined
Medium 184
III. Preparation of RNA and cDNA 186
IV. Polymerase Chain Reaction 187
V. Selection of Primers 187
VI. Specificity of Primers 189
VII. Effects of Leukemia Inhibitory Factor, Ciliary Neurotrophic Factor, and
Depolarization on Cultured Sympathetic Neurons 191
VIII. Closing Comments 193
References 193
11 Selective Aggregation Assays for Embryonic Brain Cells and Cell Lines
Shinichi Nakagawa, Hiroaki Matsunami, and Masatoshi Takeichi
I. Introduction 197
II. Principles of How to Disaggregate and Reaggregate Cells 199
III. Solutions 200
IV. Preparation of Single Cell Suspensions 201
V. Aggregation of Cells 205
VI. Analyzing the Results 207
References 209
12 Flow Cytometric Analysis of Whole Organs and Embryos
Jose Serna, Belen Pimentel, and Enrique ). de la Rosa
I. Introduction 211 i
II. Specific Considerations for Whole Organs and Embryos 212
III. Selected Applications 216
IV. Concluding Remarks 220
References 221
Contents ix
13 Detection of Multiple Gene Products Simultaneously by in Situ
Hybridization and Immunohistochemistry in Whole Mounts of
Avian Embryos
Claudio D. Stern
I. Introduction 223
II. Synthesis of Digoxigenin and Fluorescein Labeled Riboprobes by in Vitro
Transcription 224
III. Preparation of Embryos 227
IV. Prehybridization, Hybridization, and Post Hybridization Washes 228
V. Antibody Incubations 230
VI. Detection 231
VII. Timetable for Multiple Color Detection 236
VIII. Photography 237
IX. Histologic Sectioning of Whole Mounts 237
X. Designing Appropriate Controls 238
XI. Trouble Shooting 239
References 242
14 Cloning of Genes from Single Neurons
Catherine Dulac
I. Introduction: The Cloning of Mammalian Pheromone Receptor Genes 245
II. Tissue Dissociation and Isolation of Single Neurones 249
III. cDNA Synthesis and Amplification 251
IV. Differential Screening of Single Cell cDNA Libraries 254
V. Concluding Remarks 257
References 258
15 Methods for Detecting and Quantifying Apoptosis
Nicola ). McCarthy and Gerard I. Evan
I. Introduction 259
II. The Problems of Measuring Apoptosis 260
III. Methods for Identifying and Measuring Apoptosis 262
IV. Practical Methods for Detecting and Measuring Apoptosis 263
References 277
X Contents
16 Methods in Drosophila Cell Cycle Biology
Fabian Feiguin, Salud Llamazares, and Cayetano Gonzalez
I. Introduction 279
II. Fluorescent in Situ Hybridization to Polytene Chromosomes: Protocol 1 280
III. Fluorescent in Situ Hybridization to Diploid Cells: Protocol 2 282
IV. Immunostaining Embryos: Protocol 3 284
V. Immunostaining Larval Neuroblasts: Protocol 4 286
VI. Immunostaining of Female Meiotic Spindles: Protocol 5 288
VII. Preparing Drosophila Neuroblast Primary Cultures: Protocol 6 289
References 291
17 Single Central Nervous System Neurons in Culture
Juan Lerma, Miguel Morales, and Maria de los Angeles Vicente
I. Introduction 293
II. Microculture Preparation: Steps to Follow 294
III. Examples on the Functionality of Microcultured Neurons 296
References 301
18 Patch Clamp Recordings from Drosophila Presynaptic Terminals
Manuel Martinez Padron and Alberto Ferrus
I. Introduction 303
II. Larval Preparation and Dissection 305
III. Synaptic Boutons 305
IV. Electrophysiological Recordings 306
V. Technical Considerations 307
VI. Perspectives 310
References 311
Index 313
Contents of Previous Volumes 325
t
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| series2 | Current topics in developmental biology |
| spelling | Cellular and molecular procedures in developmental biology ed. by Flora de Pablo ... San Diego [u.a.] Acad. Press 1998 XVI, 329 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Current topics in developmental biology 36 Embryonic Development cabt Molecular Biology cabt Biologie du développement - Technique Biologie moléculaire - Technique Cytologie - Technique Ontwikkelingsbiologie gtt Cytological Techniques Cytology Methodology Developmental Biology methods Developmental biology Methodology Molecular Biology methods Molecular biology Methodology Entwicklungsbiologie (DE-588)4152440-8 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Zelle (DE-588)4067537-3 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Entwicklungsbiologie (DE-588)4152440-8 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Zelle (DE-588)4067537-3 s Pablo, Flora de Sonstige oth Current topics in developmental biology 36 (DE-604)BV002685390 36 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007892900&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cellular and molecular procedures in developmental biology Current topics in developmental biology Embryonic Development cabt Molecular Biology cabt Biologie du développement - Technique Biologie moléculaire - Technique Cytologie - Technique Ontwikkelingsbiologie gtt Cytological Techniques Cytology Methodology Developmental Biology methods Developmental biology Methodology Molecular Biology methods Molecular biology Methodology Entwicklungsbiologie (DE-588)4152440-8 gnd Molekularbiologie (DE-588)4039983-7 gnd Zelle (DE-588)4067537-3 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4152440-8 (DE-588)4039983-7 (DE-588)4067537-3 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Cellular and molecular procedures in developmental biology |
| title_auth | Cellular and molecular procedures in developmental biology |
| title_exact_search | Cellular and molecular procedures in developmental biology |
| title_full | Cellular and molecular procedures in developmental biology ed. by Flora de Pablo ... |
| title_fullStr | Cellular and molecular procedures in developmental biology ed. by Flora de Pablo ... |
| title_full_unstemmed | Cellular and molecular procedures in developmental biology ed. by Flora de Pablo ... |
| title_short | Cellular and molecular procedures in developmental biology |
| title_sort | cellular and molecular procedures in developmental biology |
| topic | Embryonic Development cabt Molecular Biology cabt Biologie du développement - Technique Biologie moléculaire - Technique Cytologie - Technique Ontwikkelingsbiologie gtt Cytological Techniques Cytology Methodology Developmental Biology methods Developmental biology Methodology Molecular Biology methods Molecular biology Methodology Entwicklungsbiologie (DE-588)4152440-8 gnd Molekularbiologie (DE-588)4039983-7 gnd Zelle (DE-588)4067537-3 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Embryonic Development Molecular Biology Biologie du développement - Technique Biologie moléculaire - Technique Cytologie - Technique Ontwikkelingsbiologie Cytological Techniques Cytology Methodology Developmental Biology methods Developmental biology Methodology Molecular Biology methods Molecular biology Methodology Entwicklungsbiologie Molekularbiologie Zelle Methode Aufsatzsammlung |
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