PCR strategies
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
San Diego [u.a.]
Acad. Press
1995
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV010553632 | ||
| 003 | DE-604 | ||
| 005 | 19960130 | ||
| 007 | t | ||
| 008 | 960109s1995 ad|| |||| 00||| eng d | ||
| 020 | |a 0123721822 |9 0-12-372182-2 | ||
| 020 | |a 0123721830 |9 0-12-372183-0 | ||
| 035 | |a (OCoLC)246828169 | ||
| 035 | |a (DE-599)BVBBV010553632 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-29 |a DE-355 |a DE-20 |a DE-19 |a DE-29T |a DE-11 | ||
| 082 | 0 | |a 574.873282 | |
| 084 | |a WC 4350 |0 (DE-625)148095: |2 rvk | ||
| 084 | |a WG 1750 |0 (DE-625)148497: |2 rvk | ||
| 245 | 1 | 0 | |a PCR strategies |c ed. by Michael A. Innis ... |
| 264 | 1 | |a San Diego [u.a.] |b Acad. Press |c 1995 | |
| 300 | |a XV, 373 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 0 | 7 | |a Genamplifikation |0 (DE-588)4333686-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Polymerasen |0 (DE-588)4175227-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Polymerasen |0 (DE-588)4175227-2 |D s |
| 689 | 0 | 1 | |a Genamplifikation |0 (DE-588)4333686-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a Innis, Michael A. |e Sonstige |4 oth | |
| 856 | 4 | 2 | |m HEBIS Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007035474&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-007035474 | ||
Datensatz im Suchindex
| _version_ | 1804125022698078208 |
|---|---|
| adam_text | PCR
STRATEGIES
Edited by
Michael A Innis
Chiron Corporation, Emeryville, California
David H Gelfand and John J Sninsky
Roche Molecular Systems, Inc , Alameda, California
ACADEMIC PRESS
San Diego New York Boston London Sydney Tokyo Toronto
CONTENTS
Contributors ix
Foreword xiii
Preface xv
Part One
KEY CONCEPTS FOR PCR
1 The Use of Cosolvents to Enhance Amplification by the
Polymerase Chajn Reaction 3
P A Landie, amp; H^6elfand, and R M Watson
2 DNA Polymerase Fidelity: Misinsertions and
Mismatched Extensions 17
Myron F Goodman
3 Extraction of Nucleic Acids: Sample Preparation from
Paraffin-Embedded Tissues 32
Hiroko Shimizu and fane C Burns
4 Thermostable DNA Polymerases 39
Richard D Abramson
5 Amplification of RNA: High-Temperature Reverse
Transcription and DNA Amplification with Thermus
theimophilus DNA Polymerase 58
Thomas W Myers and Christopher L Sigua
6 Nucleic Acid Hybridization and Unconventional
Bases 69
fames G Wetmur and fohn f Sninsky
vi Contents
) 7 Practical Considerations for the Design of Quantitative
PCR Assays 84
Robert Diaco
Part Two
ANALYSIS OF PCR PRODUCTS
8 Carrier Detection of Cystic Fibrosis Mutations Using
PCR-Amplified DNA and a Mismatch-Binding
Protein, MutS 111
Alia Lishanski and Jasper Rine
9 Single-Stranded Conformational Polymorphisms 121
Anne L Bailey
10 Analysis of PCR Products by Covalent Reverse Dot Blot
Hybridization 130
Farid F Chehab, Jeff Wall, and Shi-ping Cai
11 High-Performance Liquid Chromatography Analysis of
PCR Products 140
John M Wages, Xumei Zhao, and Elena D Katz
12 Heteroduplex Mobility Assays for Phylogenetic
Analysis 154
Eric L Delwart, Eugene G Shpaer, and James I Mullins
13 PCR Amplification of VNTRs 161
S Scharf
Part Three
RESEARCH APPLICATIONS
14 Site-Specific Mutagenesis Using the Polymerase Chain
Reaction 179
Jonathan Silver, Teresa Limjoco, and Stephen Feinstone
Contents vii
15 Exact Quantification of DNA-RNA Copy Numbers by
PCR-TGGE 189
Jie Kang, Peter Schdfer, foachim E Kiihn,
Andreas Immelmann, and Karsten Henco
16 The in Situ PCR: Amplication and Detection of DNA
in a Cellular Context 199
Ernest Retzel, Katherine A Staskus, Janet E Embretson, and
Ashley T Haase
17 Y Chromosome-Specific PCR: Maternal Blood 213
Diana W Bianchi
18 Genomic Subtraction 220
Don Straus
19 DNA Amplification-Restricted Transcription
Translation DARTTy Analysis of in Vitro and in Situ
Protein Functions and Intermolecular Assembly 237
Erich R Mackow
20 DNA and RNA Fingerprinting Using Arbitrarily
Primed PCR 249
John Welsh, David Ralph, and Michael McClelland
21 PCR-Based Screening of Yeast Artificial Chromosome
Libraries 277
Eric D Green
22 Oligonucleotide Ligands That Discriminate between
Theophylline and Caffeine 289
Rob Jenison, Stanley Gill, Jr , and Barry Polisky
23 Generation of Single-Chain Antibody Fragments
by PCR 300
Jeffrey R Stinson, Vaughan Wittman, and Hing C Wong
24 Longer PCR Amplifications 313
Suzanne Cheng
viii Contents
25 Direct Analysis of Specific Bonds from Arbitrarily
Primed PCR Reactions 325
D A Carter, A Burt, and J W Taylor
Part Four
ALTERNATIVE AMPLIFICATION STRATEGIES
26 Detection of Leber s Hereditary Optic Neuropathy by
Nonradioactive Ligase Chain Reaction 335
John A Zebala and Francis Barony
27 Detection of listeiia monocytogenes by PCR-Coupled
Ligase Chain Reaction 347
Martin Wiedmann, Francis Barony, and Carl A Batt
Index 363
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV010553632 |
| classification_rvk | WC 4350 WG 1750 |
| ctrlnum | (OCoLC)246828169 (DE-599)BVBBV010553632 |
| dewey-full | 574.873282 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] |
| dewey-raw | 574.873282 |
| dewey-search | 574.873282 |
| dewey-sort | 3574.873282 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01575nam a2200409 c 4500</leader><controlfield tag="001">BV010553632</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">19960130 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">960109s1995 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0123721822</subfield><subfield code="9">0-12-372182-2</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0123721830</subfield><subfield code="9">0-12-372183-0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)246828169</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV010553632</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-11</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">574.873282</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4350</subfield><subfield code="0">(DE-625)148095:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 1750</subfield><subfield code="0">(DE-625)148497:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">PCR strategies</subfield><subfield code="c">ed. by Michael A. Innis ...</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">San Diego [u.a.]</subfield><subfield code="b">Acad. Press</subfield><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XV, 373 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genamplifikation</subfield><subfield code="0">(DE-588)4333686-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polymerasen</subfield><subfield code="0">(DE-588)4175227-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Polymerasen</subfield><subfield code="0">(DE-588)4175227-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Genamplifikation</subfield><subfield code="0">(DE-588)4333686-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Innis, Michael A.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HEBIS Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007035474&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-007035474</subfield></datafield></record></collection> |
| id | DE-604.BV010553632 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:54:57Z |
| institution | BVB |
| isbn | 0123721822 0123721830 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007035474 |
| oclc_num | 246828169 |
| open_access_boolean | |
| owner | DE-29 DE-355 DE-BY-UBR DE-20 DE-19 DE-BY-UBM DE-29T DE-11 |
| owner_facet | DE-29 DE-355 DE-BY-UBR DE-20 DE-19 DE-BY-UBM DE-29T DE-11 |
| physical | XV, 373 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| publisher | Acad. Press |
| record_format | marc |
| spelling | PCR strategies ed. by Michael A. Innis ... San Diego [u.a.] Acad. Press 1995 XV, 373 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Genamplifikation (DE-588)4333686-3 gnd rswk-swf Polymerasen (DE-588)4175227-2 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Polymerasen (DE-588)4175227-2 s Genamplifikation (DE-588)4333686-3 s DE-604 Polymerase-Kettenreaktion (DE-588)4256726-9 s Innis, Michael A. Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007035474&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | PCR strategies Genamplifikation (DE-588)4333686-3 gnd Polymerasen (DE-588)4175227-2 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4333686-3 (DE-588)4175227-2 (DE-588)4256726-9 |
| title | PCR strategies |
| title_auth | PCR strategies |
| title_exact_search | PCR strategies |
| title_full | PCR strategies ed. by Michael A. Innis ... |
| title_fullStr | PCR strategies ed. by Michael A. Innis ... |
| title_full_unstemmed | PCR strategies ed. by Michael A. Innis ... |
| title_short | PCR strategies |
| title_sort | pcr strategies |
| topic | Genamplifikation (DE-588)4333686-3 gnd Polymerasen (DE-588)4175227-2 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Genamplifikation Polymerasen Polymerase-Kettenreaktion |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007035474&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT innismichaela pcrstrategies |