Plant cell wall analysis
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| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1996
|
| Schriftenreihe: | Modern methods of plant analysis
New series ; 17 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
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| 245 | 1 | 0 | |a Plant cell wall analysis |c ed. by H. F. Linskens ... Contrib. A. Bacic ... |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1996 | |
| 300 | |a XVII, 205 S. |b graph. Darst. | ||
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Datensatz im Suchindex
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| adam_text | PLANT CELL WALL ANALYSIS EDITED BY H.F. LINSKENS AND J.F. JACKSON
CONTRIBUTORS A. BACIC L.M. BARONE M. BORDENAVE H. CHANZY M.A. COIMBRA I.
DELGADILLO K. EL-BAHTIMY L. EPSTEIN M. FEVRE M.L. FISHMAN P.P. GALLEGO
L. GAY E.E. HOOD A.T. HOTCHKISS I.M. MORRISON W.M.A. NIESSEN X. QI S.M.
READ H.A. SCHOLS R.R. SELVENDRAN J. VAN DER GREEF R.A.M. VAN DER HOEVEN
A.G.J. VORAGEN V. VREELAND K.W. WALDRON B.P. WASSERMAN A. WU WITH 36
FIGURES SPRINGER CONTENTS FRACTIONATION OF CELL WALL COMPONENTS I.M.
MORRISON 1 1 INTRODUCTION 1 2 POLYSACCHARIDES 1 2.1 PRECIPITATION
REACTIONS 2 2.1.1 ADJUSTMENT OF PH 2 2.1.2 PRECIPITATION WITH ORGANIC
SOLVENTS 2 2.1.3 PRECIPITATION WITH INORGANIC SALTS 3 2.1.4
PRECIPITATION WITH IODINE 4 2.1.5 PRECIPITATION WITH IONIC DETERGENTS 5
2.2 CHROMATOGRAPHY 5 2.2.1 SIZE CHROMATOGRAPHY 5 2.2.2 ION EXCHANGE
CHROMATOGRAPHY 7 2.2.3 AFFINITY CHROMATOGRAPHY 9 2.3 ELECTROPHORESIS 10
2.3.1 MOVING BOUNDARY ELECTROPHORESIS 10 2.3.2 PAPER ELECTROPHORESIS 11
2.4 MISCELLANEOUS POLYSACCHARIDE METHODS 11 2.4.1 CELLULOSE 11 2.4.2
POLYSACCHARIDE DERIVATIVES 11 3 PROTEINS AND GLYCOPROTEINS 12 3.1
PRECIPITATION REACTIONS 13 3.1.1 PRECIPITATION WITH AMMONIUM SULPHATE 13
3.1.2 PRECIPITATION WITH TRICHLOROACETIC ACID 13 3.2 CHROMATOGRAPHY 13
3.2.1 SIZE CHROMATOGRAPHY 13 3.2.2 ION EXCHANGE CHROMATOGRAPHY 13 3.3
ELECTROPHORESIS 14 3.3.1 SODIUM DODECYLSULPHATE-POLYACRYLAMIDE GEL
ELECTROPHORESIS .. 14 3.3.2 ISOELECTRIC FOCUSING 14 4 LIGNINS 15
REFERENCES 16 ISOLATION AND ANALYSIS OF CELL WALL POLYMERS FROM OLIVE
PULP M.A. COIMBRA, I. DELGADILLO, K.W. WALDRON, AND R.R. SELVENDRAN ..
19 X CONTENTS 1 INTRODUCTION 19 2 ISOLATION OF CELL WALLS FROM OLIVE
PULP 19 2.1 PREPARATION AND USE OF ALCOHOL-INSOLUBLE RESIDUE (AIR) -
GENERAL CONSIDERATIONS 20 2.2 PREPARATION OF CELL WALL MATERIAL (CWM) 21
2.2.1 MATERIAL SOLUBILIZED DURING THE PREPARATION OF CWM 23 3 SEQUENTIAL
EXTRACTION OF CELL WALL POLYMERS 24 3.1 GENERAL CONSIDERATIONS 24 3.2
SEQUENTIAL EXTRACTION 24 3.2.1 GENERAL COMMENTS TO THE METHOD 27 4
FRACTIONATION OF THE EXTRACTED CELL WALL POLYSACCHARIDES 29 4.1 GENERAL
CONSIDERATIONS 29 4.2 GRADED PRECIPITATION WITH ETHANOL 30 4.3
ANION-EXCHANGE CHROMATOGRAPHY 30 4.3.1 ANION-EXCHANGE CHROMATOGRAPHY OF
PECTIC POLYSACCHARIDES ... 32 4.3.2 ANION-EXCHANGE CHROMATOGRAPHY OF
HEMICELLULOSIC POLYSACCHARIDES 33 4.4 FRACTIONATION OF ACIDIC XYLANS 34
5 CHEMICAL AND SPECTROSCOPIC ANALYSIS 34 5.1 NEUTRAL SUGARS 34 5.2
URONIC ACID 35 5.3 HYDROXYPROLINE ESTIMATION 36 5.4 METHYLATION ANALYSIS
36 5.4.1 HAKOMORI METHYLATION 36 5.4.2 CIUCANU AND KEREK METHYLATION 36
5.4.3 CARBOXYL-REDUCTION OF METHYLATED POLYSACCHARIDES 37 5.4.4 COMMENTS
ON RESULTS OF METHYLATION ANALYSIS 37 5.5 13 C-NMR STUDIES ON CELL WALL
POLYSACCHARIDES 39 5.6 FOURIER TRANSFORM INFRARED SPECTROSCOPY (FTIR) 39
6 CONCLUDING REMARKS 41 REFERENCES 41 DETERMINATION OF CELL WALL
AUTOLYSIS P.P. GALLEGO 45 1 INTRODUCTION 45 2 FACTORS ALTERING CELL WALL
ISOLATION AND AUTOLYSIS 46 2.1 PLANT MATERIAL 47 2.2 BUFFERS 47 2.3 PH
48 2.4 IONIC STRENGTH ; 48 3 ISOLATION OF ACTIVE CELL WALLS 49 3.1
HOMOGENIZATION IN AQUEOUS MEDIUM 49 3.2 FILTRATION AND WASHES 50 4
ISOLATION OF INACTIVE CELL WALLS (CONTROLS) 51 CONTENTS XI 4.1 BOILED IN
HOT WATER 51 4.2 BOILED IN ALCOHOL 51 4.3 PHENOL-ACETIC ACID-WATER 52
4.4 BUFFER PHENOL AT PH 7 52 4.5 LOW TEMPERATURES 53 5 AUTOLYSIS
INCUBATIONS 53 5.1 OPTIMIZATION OF THE AUTOLYSIS REACTION 54 5.2 GENERAL
PROCEDURE 54 6 ANALYSIS OF AUTOLYSIS PRODUCTS 56 6.1 GENERAL METHODS 56
6.2 ETHANOL PRECIPITATION 56 6.3 GEL PERMEATION CHROMATOGRAPHY 57 6.4
ION EXCHANGE CHROMATOGRAPHY 57 7 CONCLUSIONS 57 REFERENCES 58 CELL WALL
POROSITY AND ITS DETERMINATION S.M. READ AND A. BACIC 63 1 INTRODUCTION
63 1.1 BIOLOGICAL SIGNIFICANCE OF WALL POROSITY 63 1.2 ASSUMPTIONS AND
DEFINITIONS 64 2 MICROSCOPIC VISUALIZATION OF WALL PORES 66 3 BULK
EXCLUSION TECHNIQUES 67 3.1 SOLUTE EXCLUSION 67 3.2 LONG-TERM-EXCLUSION
OF PEG (POLYETHYLENGLYCOL) 69 3.3 GEL FILTRATION 70 4 TRACER TECHNIQUES:
UPTAKE OF MOLECULES OR PARTICLES 71 4.1 SMALL MOLECULES 71 4.2
MACROMOLECULES 72 4.3 PARTICLES 75 5 CONCLUSIONS 75 5.1 INTEGRATION OF
RESULTS FROM DIFFERENT METHODS 75 5.2 PORE STRUCTURE 76 5.3 VARIATIONS
IN WALL POROSITY 77 5.4 FUTURE DEVELOPMENTS 78 REFERENCES 78 ANALYSIS OF
CHITIN BIOSYNTHESIS M. FEVRE, L. GAY, AND H. CHANZY 81 1 INTRODUCTION 81
2 STRUCTURE OF CHITIN ;. 81 3 THE ENZYMATIC SYNTHESIS OF CHITIN 82 XII
CONTENTS 3.1 ASSAY OF CHITIN SYNTHASE 83 3.2 INHIBITION OF ENZYMATIC
ACTIVITY 83 4 PREPARATION OF CHITIN SYNTHASE 84 5 PRODUCT
CHARACTERIZATION OF CHITIN SYNTHASE 85 5.1 CHEMICAL IDENTIFICATION 85
5.2 BIOPHYSICAL IDENTIFICATION OF CHITIN 86 5.2.1 FOURIER TRANSFORM
INFRARED SPECTROSCOPY 86 5.2.2 X-RAY DIFFRACTION ANALYSIS 86 5.2.3
ELECTRON MICROSCOPY AND ELECTRON DIFFRACTION ANALYSIS 88 6
POLYMERIZATION AND CRYSTALLIZATION 90 7 FUNGAL CHITIN SYNTHASE GENES 90
8 MORPHOGENETIC ROLES OF CHITIN SYNTHASES 91 9 CONCLUSIONS 92 REFERENCES
92 ANALYSIS OF PLANT-SUBSTRATUM ADHESIVES V. VREELAND AND L. EPSTEIN 95
1 INTRODUCTION 95 1.1 PRINCIPLES OF ADHESION 95 1.2 CELL-SUBSTRATUM
ADHESION 96 1.2.1 HIGHER PLANT-SUBSTRATUM ADHESION 98 1.2.2 ADHESION OF
FUNGAL PHYTOPATHOGENS TO A PLANT SUBSTRATUM ... 98 1.2.3
ALGAL-SUBSTRATUM ADHESION 99 2 CASE STUDIES OF CELL-SUBSTRATUM ADHESION
100 2.1 SUBSTRATE ADHESION BY THE FUCUS ZYGOTE 100 2.2 ADHESION OF
CONIDIOSPORES OF THE PLANT PATHOGENIC FUNGUS, NECTRIA HAEMATOCOCCA 103 3
METHODS FOR THE ANALYSIS OF CELL-SUBSTRATUM ADHESIVES 104 3.1 ADHESION
SYSTEMS AND ASSAYS 104 3.2 IDENTIFICATION OF ADHESIVE COMPONENTS 105
3.2.1 ISOLATION OF ADHESION MUTANTS 105 3.2.2 CORRELATION OF
TEMPORAL/SPATIAL DEVELOPMENT WITH ADHESION 106 3.2.3 EXPERIMENTAL
PERTURBATION OF ADHESION 107 3.3 ANALYSIS OF ADHESIVE COMPONENTS 108
3.3.1 EXTRACTION AND PURIFICATION 108 3.3.2 ASSAYS OF ISOLATED COMPOUNDS
FOR ADHESIVE ACTIVITY 109 4 CONCLUSIONS 110 REFERENCES ILL BIOCHEMICAL,
IMMUNOLOGICAL AND MOLECULAR ANALYSES OF EXTENSIN E.E. HOOD 117 CONTENTS
XIII 1 INTRODUCTION 117 2 BIOCHEMICAL CHARACTERIZATION OF EXTENSIN 117
2.1 PREPARATION OF WALLS 117 2.2 PROTEIN PURIFICATION 118 2.3
BIOCHEMICAL CHARACTERIZATION 119 3 IMMUNOLOGICAL DETECTION OF EXTENSIN
120 3.1 IN VIVO LOCALIZATION OF EXTENSIN 121 3.2 WESTERN BLOTS AND
TISSUE PRINTS 121 4 MOLECULAR CHARACTERIZATION OF EXTENSIN 122 4.1
ISOLATION OF GENES ENCODING EXTENSIN 123 4.2 EXTENSIN EXPRESSION STUDIES
125 4.3 EXTENSIN PROMOTER FUSIONS WITH REPORTER GENES 125 4.4 GENERATION
OF EXTENSIN MUTANTS 126 5 SUMMARY 126 REFERENCES 126 ANALYSIS OF PECTIN
STRUCTURE BY HPAEC-PAD A.T. HOTCHKISS, JR., K. EL-BAHTIMY, AND M.L.
FISHMAN 129 1 INTRODUCTION 129 2 PECTIN HPLC SEPARATIONS 130 2.1 GPC 130
2.2 ION-EXCHANGE AND ION-PAIR RP 130 2.3 HPAEC-PAD 131 3 PECTIN ANALYSIS
IN RIPENING PEACH FRUIT 134 3.1 MELTING- AND NONMELTING-FLESH PEACHES
134 3.2 HPAEC-PAD SYSTEM 135 3.3 SAMPLE PREPARATION 136 3.4 HPAEC-PAD OF
REDSKIN AND SUNCLING PEACH PECTIN 136 4 CONCLUSIONS AND FUTURE
DIRECTIONS 142 REFERENCES 142 CHARACTERIZATION OF OLIGOSACCHARIDES
DERIVED FROM PLANT CELL WALL POLYSACCHARIDES BY ON-LINE HIGH-PERFORMANCE
ANION-EXCHANGE CHROMATOGRAPHY THERMOSPRAY MASS SPECTROMETRY W.M.A.
NIESSEN, H.A. SCHOLS, R.A.M. VAN DER HOEVEN, A.G.J. VORAGEN, AND J. VAN
DER GREEF 147 1 INTRODUCTION 147 2 METHODS 148 2.1 APPARATUS 148 2.2
ISOLATION OF OLIGOSACCHARIDES FROM PLANT CELL WALL MATERIAL 150 3 HPAEC
IN OLIGOSACCHARIDE ANALYSIS 150 XIV CONTENTS 4 GENERAL EXPERIMENTAL
CONSIDERATIONS RELATED TO HPAEC-MS 152 4.1 DESALTING BY AMMS 152 4.2
IONIZATION OF OLIGOSACCHARIDES 153 4.3 DATA INTERPRETATION 155 5
APPLICATION OF HPAEC-MS IN OLIGOSACCHARIDE CHARACTERIZATION 156 6
CONCLUSIONS AND PERSPECTIVES 160 REFERENCES 162 ANALYSIS OF PECTIN
METHYL ESTERASES M. BORDENAVE 165 1 INTRODUCTION 165 2 ESTIMATION AND
DETECTION OF PME ACTIVITIES 165 2.1 DETECTION 165 2.2 ESTIMATION 167 3
PME LOCALIZATION 167 4 PME EXTRACTION AND PURIFICATION 168 5 PME
PROPERTIES 169 5.1 PHYSICOCHEMICAL PROPERTIES 169 5.2 ENZYMIC PROPERTIES
171 5.2.1 ACTION PATTERN 171 5.2.2 INFLUENCE OF PH ON PME ACTIVITY 172
5.2.3 INFLUENCE OF CATIONS 174 5.3 STRUCTURES 174 6 ROLES OF PMES 175
6.1 PLANT PATHOGENS 175 6.2 FRUIT MATURATION 176 6.3 CELL ELONGATION 176
7 CONCLUSIONS AND PERSPECTIVES : 177 REFERENCES 177 PROBING THE SUBUNIT
COMPOSITION AND TOPOLOGY OF PLASMA MEMBRANE-BOUND (L,3)-(3-GLUCAN
(CALLOSE) SYNTHASES B.P. WASSERMAN, XIAOQUN QI, L.M. BARONE, AND AYONG
WU 181 1 INTRODUCTION 181 2 MEMBRANE ISOLATION, ENZYME ASSAY, AND
SOLUBILIZATION 183 2.1 ISOLATION OF CRUDE MEMBRANE FRACTIONS 183 2.2
PLASMA MEMBRANE ISOLATION 185 2.3 MEMBRANES OF DEFINED SIDEDNESS: INSIDE
OUT AND RIGHT-SIDE OUT VESICLES 185 2.4 CALLOSE SYNTHASE ASSAY 185 2.5
CALLOSE SYNTHASE SOLUBILIZATION 186 CONTENTS XV 3 CALLOSE SYNTHASE
TOPOLOGY, PURIFICATION, AND SUBUNIT COMPOSITION 187 3.1 POLYPEPTIDE
DEPLETION 187 3.2 VECTORIAL PROTEOLYSIS 188 3.3 CALLOSE SYNTHASE
PURIFICATION 189 3.3.1 GLYCEROL GRADIENT CENTRIFUGATION 189 3.3.2
PRODUCT ENTRAPMENT 191 4 BIOCHEMICAL CHARACTERIZATION OF INTEGRAL PLANT
PLASMA MEMBRANE PROTEINS 191 4.1 CHARACTERIZATION OF DISULFIDE-LINKED
AGGREGATE FORMATION 191 4.2 RECOVERY OF HYDROPHOBIC PLANT-DERIVED
MEMBRANE PROTEINS FOR SEQUENCING AND ANTIBODY PRODUCTION 192 4.3
ANTIBODY CHARACTERIZATION 193 4.3.1 IMMUNOBLOTTING 194 4.3.2 AFFINITY
PURIFICATION OF ANTI-PMIP27 194 5 SUMMARY 195 REFERENCES 196 SUBJECT
INDEX 199
|
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| genre | (DE-588)4143389-0 Aufgabensammlung gnd-content |
| genre_facet | Aufgabensammlung |
| id | DE-604.BV010494146 |
| illustrated | Illustrated |
| indexdate | 2025-01-08T13:50:52Z |
| institution | BVB |
| isbn | 354059406X 038759406X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006992878 |
| oclc_num | 35784647 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-12 DE-703 DE-29T DE-20 DE-83 DE-11 |
| owner_facet | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-355 DE-BY-UBR DE-12 DE-703 DE-29T DE-20 DE-83 DE-11 |
| physical | XVII, 205 S. graph. Darst. |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| publisher | Springer |
| record_format | marc |
| series | Modern methods of plant analysis |
| series2 | Modern methods of plant analysis : New series |
| spellingShingle | Plant cell wall analysis Modern methods of plant analysis Celwanden gtt Citologia e biologia celular larpcal Citologia vegetal larpcal Planten gtt Plant cell walls Analysis Zellwand (DE-588)4190675-5 gnd Analyse (DE-588)4122795-5 gnd Chemische Analyse (DE-588)4009840-0 gnd Pflanzen (DE-588)4045539-7 gnd |
| subject_GND | (DE-588)4190675-5 (DE-588)4122795-5 (DE-588)4009840-0 (DE-588)4045539-7 (DE-588)4143389-0 |
| title | Plant cell wall analysis |
| title_auth | Plant cell wall analysis |
| title_exact_search | Plant cell wall analysis |
| title_full | Plant cell wall analysis ed. by H. F. Linskens ... Contrib. A. Bacic ... |
| title_fullStr | Plant cell wall analysis ed. by H. F. Linskens ... Contrib. A. Bacic ... |
| title_full_unstemmed | Plant cell wall analysis ed. by H. F. Linskens ... Contrib. A. Bacic ... |
| title_short | Plant cell wall analysis |
| title_sort | plant cell wall analysis |
| topic | Celwanden gtt Citologia e biologia celular larpcal Citologia vegetal larpcal Planten gtt Plant cell walls Analysis Zellwand (DE-588)4190675-5 gnd Analyse (DE-588)4122795-5 gnd Chemische Analyse (DE-588)4009840-0 gnd Pflanzen (DE-588)4045539-7 gnd |
| topic_facet | Celwanden Citologia e biologia celular Citologia vegetal Planten Plant cell walls Analysis Zellwand Analyse Chemische Analyse Pflanzen Aufgabensammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006992878&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035361205 |
| work_keys_str_mv | AT linskenshansf plantcellwallanalysis AT bacicantony plantcellwallanalysis |