Biothiols A Monothiols and dithiols, protein thiols, and thiyl radicals

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Veröffentlicht: San Diego, Calif. u.a. Acad. Press 1995
Schriftenreihe:Methods in enzymology 251
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Datensatz im Suchindex

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adam_text Table of Contents Contributors to Volume 251 ix Preface xiii Volumes in Series . xv Overview 1. Glutathione Metabolism Alton Meister 3. 2. Thiol/Disulfide Exchange Equilibria and Disulflde Hiram F. Gilbert 8 Bond Stability Section I. Thiyl Radicals 3. Kinetic Factors That Control the Fate of Thiyl Peter Wardman and Radicals in Cells Clemens von Sonntag 31 4. Kinetics of Thiol Reactions Christian Schoneich 45 5. Perthiols as Antioxidants: Radical Scavenging and Steven A. Everett and Pro oxidative Mechanisms Peter Wardman 55 6. Thiyl (Sulfhydryl/Thiol) Free Radical Reactions, Subhas C. Kundu and Vitamins, /3 Carotene, and Superoxide Dismu Robin L. Willson 69 tase in Oxidative Stress: Design and Interpreta¬ tion of Enzymatic Studies 7. Reaction of Superoxide with Glutathione and Christine C. Winterbourn Other Thiols and Diana Metodiewa 81 8. Quantitative Determination of Thiol Groups in Lev M. Weiner 87 Low and High Molecular Weight Compounds by Electron Paramagnetic Resonance 9. Thiyl Radical Formation during Thiol Oxidation Enrique Cadenas 106 by Ferrylmyoglobin 10. /«V7i;oToxicity of Thiols: Relationship to Rate of Rex Munday 117 One Electron Oxidation by Oxyhemoglobin v Vi TABLE OF CONTENTS Section II. Chemical Basis of Thiol/Disulfide Measurements 11. Diamide: An Oxidant Probe for Thiols Nechama S. Kosower and Edward M. Kosower 123 12. Bromobimane Probes for Thiols Edward M. Kosower and Nechama S. Kosower 133 13. Determination of Biothiols by Bromobimane La Gerald L. Newton and beling and High Performance Liquid Chroma Robert C. Fahey 148 tography 14. Reagents for Rapid Reduction of Disulfide Bonds Rajeeva Singh, Guy V. Lamoureux, Watson J. Lees, and George M. Whitesides 167 15. l,3 Bis(2 chloroethyl) l nitrosourea as Thiol Car Katja Becker and bamoylating Agent in Biological Systems R. Heiner Schirmer 173 Section III. Monothiols: Measurement in Organs, Cells, Organelles, and Body Fluids 16. Noninvasive Measurement of Thiol Levels in Hans Nohl, Cells and Isolated Organs Klaus Stolze, and Lev M. Weiner 191 17. Measurement of Thiols in Cell Populations from John A. Cook and Tumor and Normal Tissue James B. Mitchell 203 18. Measurement of Glutathione Redox State in Cyto Christopher Hwang, sol and Secretory Pathway of Cultured Cells Harvey F. Lodish, and Anthony J. Sinskey 212 19. Assay of Thiols and Disulfldes in Intestinal Lymph Tak Yee Aw 221 20. Assay for Thiols Based on Reactivation of Papain Rajeeva Singh, Walter A. Blattler, and Albert R. Collinson 229 21. Assay of Blood Glutathione Oxidation during Jose1 Vina, Physical Exercise Juan Sastre, Miguel Asensi, and Lester Packer 237 . 22. X Ray Structure Methods for Glutathione Binding Peter Reinemer, Heini W. Dirr, and Robert Huber 243 23. Thiols and the Immune System: Effect of Wulf Droge, Af Acetylcysteine on T Cell System in Human Ralf Kinscherf, Subjects Sabine Mihm, Dagmar Galter, Steffen Roth, Helmut GmCnder, Thomas Fischbach, and Michael Bockstette 255 . TABLE OF CONTENTS Vii 24. Flow Cytometry Technique for Assessing Effects Ren£ Olivier 270 of Af Acetylcysteine on Apostosis and Cell Via¬ bility of Human Immunodeficiency Virus In¬ fected Lymphocytes 25. Use of p Nitrophenyl Disulfide to Measure Reduc Carlos Gitler and •; tive Capacity of Intact Cells Mauricio Londner 279 f 26. Trypanothione and iV Glutathionylspermidine: R. L. Krauth Siegel, r Isolation and Determination E. M. Jacoby, and | R. H. Schirmer 287 27. Estimation of Tissue Cysteamine by Quantitative D. M. Ziegler and Thin Layer Chromatography L. L. Poulsen 294 Section IV. Dithiols: a Lipoic Acid i 28. Reaction of Lipoic Acid with Ebselen and Hy Gerreke P. Biewenga and i pochlorous Acid Aalt Bast 303 29. Analysis of Reduced and Oxidized Lipoic Acid in Derick Han, t Biological Samples by High Performance Liq Garry J. Handelman, • uid Chromatography and Lester Packer 315 I 30. Reaction of (/?,S) Dihydrolipoic Acid and Homo Teruyuki Kawabata, j logs with Iron Hans JCrgen Tritschler, and Lester Packer 325 31. ATP Synthesis and ATPase Activities in Heart Guido Zimmer, Mitoplasts under Influence of R and 5 Enantio Luise Mainka, and 1 mers of Lipoic Acid Heinz Ulrich 332 ; 32. Assay for Protein Lipoylation Reaction Kazuko Fujiwara, Kazuko Okamura Ikeda, and yutaro motokawa 340 Section V. Protein Thiols and Sulfides 33. Chemical Modification of Protein Thiols: Forma Richard Wynn and tion of Mixed Disulfides Frederic M. Richards 351 34. Reversible Introduction of Thiol Compounds into Heinz Faulstich and Proteins by Use of Activated Mixed Disulfides Daniela Heintz 357 35. Use ofCationic Detergents to Enhance Reactivity Carlos Gitler, of Protein Sulfhydryls Batia Zarmi, and Edna Kalef 366 36. Measuring Thiol Disulfide Exchange Equilibrium Richard Wynn and Constants for Single Cysteine Containing Pro Frederic M. Richards 375 teins 37. Structure, Properties, Mechanisms, and Assays of Nobuhiko Katunuma and Cysteine Protease Inhibitors: Cystatins and Eiki Kominami 382 E 64 Derivatives I Vlii TABLE OF CONTENTS 38. Protein Disulfide Isomerase Robert B. Freedman, Hilary C. Hawkins, and Stephen H. McLaughlin 397 39. Glucocorticoid Receptor Thiols and Steroid Bind S. Stoney Simons, Jr. and ing Activity William B. Pratt 406 40. Analysis of Cells and Tissues for S Thiolation of James A. Thomas, Proteins Wei Zhao, Suzanne Hendrich, and Peter Haddock 423 41. Tertiary Structure Selective Characterization of Martin Kussmann and Protein Dithiol Groups by Phenylarsine Oxide Michael Przybylski 430 Modification and Mass Spectrometric Peptide Mapping 42. Structure and Posttranslational Modification of Li Richard N. Perham 436 poyl Domain of 2 Oxo Acid Dehydrogenase Multienzyme Complexes 43. Plasma Protein Sulfhydryl Oxidation: Effect of Albert van der Vliet, Low Molecular Weight Thiols Carroll E. Cross, Barry Halliwell, and Charles A. O Neill 448 44. Analysis of Methionine Sulfoxide in Proteins Konrad L. Maier, Anke G. Lenz, Ingrid Beck Speier, and Ulrich Costabel 455 45. Escherichia coli Peptide Methionine Sulfoxide Re Nathan Brot, ductase: Cloning, High Expression, and Purifl M. Atiqur Rahman, cation Jackob Moskovitz, Herbert Weissbach, Jeffrey Strassman, Stephanie O. Yancey, and Sidney R. Kushner 462 46. Thiol Coenzymes of Methanogens Kenneth M. Noll 470 Author Index 483 Subject Index 513
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publishDate 1995
publishDateSearch 1995
publishDateSort 1995
publisher Acad. Press
record_format marc
series Methods in enzymology
series2 Methods in enzymology
spellingShingle Biothiols
Methods in enzymology
Thiols cabt
title Biothiols
title_auth Biothiols
title_exact_search Biothiols
title_full Biothiols A Monothiols and dithiols, protein thiols, and thiyl radicals ed. by Lester Packer
title_fullStr Biothiols A Monothiols and dithiols, protein thiols, and thiyl radicals ed. by Lester Packer
title_full_unstemmed Biothiols A Monothiols and dithiols, protein thiols, and thiyl radicals ed. by Lester Packer
title_short Biothiols
title_sort biothiols monothiols and dithiols protein thiols and thiyl radicals
topic Thiols cabt
topic_facet Thiols
url http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006833366&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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