Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia]

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Veröffentlicht: New York [u.a.] Plenum Press 1990
Schriftenreihe:Monographs in evolutionary biology
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Datensatz im Suchindex

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adam_text ECOLOGICAL AND EVOLUTIONARY GENETICS OF DROSOPHILA Edited by JSF Barker The University of New England Armidale, Australia William T Starmer Syracuse University Syracuse, New York and Ross J Maclntyre Cornell University Ithaca, New York PLENUM PRESS • NEW YORK AND LONDON Contents William B Heed: A Biography James C Fogleman 1 Ecological Genetics: Introduction 15 Chapter 1 The Ecological Genetics of Abnormal Abdomen in Drosophila mercatorum Alan R Templeton, Hope Hollocher, Susan Lawler, and J Spencer Johnston 1 Introduction 17 2 The aa Syndrome in D mercatorum 18 2 1 Components of Genetic Variation in aa in Natural Populations 19 2 2 Phenotypic Expression of aa in Nature 21 2 3 The Adaptive Significance of aa 27 3 Population Structure 29 4 Testing The Adaptive Response Predictions 31 5 Conclusion 33 6 Summary 33 References 77 34 Chapter 2 Direction of Life History Evolution in Drosophila mojavensis William J Etges 1 Introduction 37 2 Materials and Methods 41 2 1 Origin of Stocks 41 2 2 Cactus Rearing Conditions 41 2 3 Population Crosses - I 42 2 4 Population Crosses - II 42 vii CONTENTS 2 5 Statistical Analysis 42 3 Results 44 3 1 Life History Differences between Baja and Mainland Populations 44 3 2 Population Crosses 46 4 Discussion 49 5 Summary 53 References 54 Chapter 3 Fitness and Asymmetry Modification as an Evolutionary Process A Study in the Australian Sheep Blowfly, Lucilia cuprina and Drosophila melanogaster John A McKenzie, Philip Batterham, and Louise Baker 1 Introduction 57 2 Resistance to Insecticides by the Australian Sheep Blowfly 58 2 1 Selection of Fitness Modifiers 59 2 2 Mapping of a Fitness Modifier in Lucilia cuprina 60 3 Departures from Symmetry: the Influence of a Resistant Allele 61 3 1 Mapping of a Fitness/Asymmetry Modifier 62 3 2 The Fitness/Asymmetry Association 63 4 The Mechanism of Modifier Action: Scl and Notch Homology? 64 4 1 The Asymmetry of Scl and Notch Phenotypes 65 4 2 The Influence of the Modifier on Scl Phenotypes 68 5 Conclusions and Future Directions 69 6 Summary 70 References 71 Chapter 4 Extreme Environmental Stress: Asymmetry, Metabolic Cost and Conservation P A Parsons 1 Introduction 2 Developmental Stress - Variability and Asymmetry 3 An Integrated Approach via Metabolic Cost 4 Is Adaptation to a Rapid Temperature Increase Possible? 5 Summary References CONTENTS ix Chapter 5 The Effects of Natural and Artificial Selection on Dysgenic Potential of a Wild Population of Drosophila melanogaster Michael Murphy, and John A Sved 1 Introduction 87 2 Methods and Materials 88 2 1 The Measurement of Dysgenesis 89 3 Results 91 3 1 Wild Populations 91 3 2 Laboratory Populations 95 4 Discussion 96 5 Summary 97 References 98 Chapter 6 P-Elements and Quantitative Variation in Drosophila Chris Moran, and Adam Torkamanzehi 1 Introduction 99 1 1 Mutation and Evolution 99 1 2 Transposable Elements 100 1 3 Quantifying the Influence of New Mutations 101 1 4 Transposition and Evolution 101 1 5 Transposition and Quantitative Variation 102 1 6 Transposition and Fitness 102 1 7 Regulation of P Element Transposition 103 2 Experimental Studies of Creation of Quantitative Variation by P Transposition 104 2 1 Crossing Protocols 104 2 2 Use of Transformed Stocks Produced by Microinjection of P Elements 110 3 Conclusions 113 4 Summary rr 114 References 115 Habitat Selection: Introduction 119 Chapter 7 Ecological and Evolutionary Importance of Host Plant Chemistry James C Fogleman, and J Ruben Abril 1 Introduction 121 2 Host Plant Relationships 121 X CONTENTS 3 Host Plant Utilization 123 4 Analysis of Fresh Cactus Tissue: Carbohydrates 125 5 Analysis of Necrotic Cactus Tissue: Volatile Compounds 132 6 Host Plant Selection 138 7 Summary 141 References 141 Chapter 8 The Nutritional Importance of Pure and Mixed Cultures of Yeasts in the Development of Drosophila mulleri Larvae in Opuntia Tissues and its Relationship to Host Plant Shifts William T Starmer, and Virginia Aberdeen 1 Introduction 145 2 Experimental Analysis 148 2 1 Strain Effects Experiment 149 2 2 Cross-Inoculation Experiments 149 2 3 Nutritional Supplement Experiment 152 3 Discussion 154 3 1 Nitrogen Limits 154 3 2 Sugars and Carbohydrates 155 3 3 Host Shifts 155 3 4 Biculture Effect 156 4 Summary 157 References 158 Chapter 9 Experimental Analysis of Habitat Selection and Maintenance of Genetic Variation JSF Barker 1 Introduction 161 2 A Strategy for Study of Heterogeneous Environments-Genetic Polymorphism Relationships 161 2 1 Comparisons Between Species 162 2 2 Comparisons Among Populations Within Species 162 2 3 Analyses Within Populations 164 3 The Cactophilic Drosophila 166 4 D buzzatii - Yeast Species Interactions 166 5 Genotype-Specific Habitat Selection 168 6 Habitat Selection in Nature 172 7 Summary 173 References 174 CONTENTS xi Chapter 10 Heritable Variation in Resource Use in Drosophila in the Field Ary A Hoffmann, and Sharon O Donnell 1 Introduction 177 2 Field Collections 180 2 1 Flies and Field Sites 180 2 2 Performance Tests 181 2 3 Resource Response 184 3 Field Releases 187 4 Discussion 188 5 Summary 191 References 192 Chapter 11 Factors Maintaining Genetic Variation For Host Preference in Drosophila John Jaenike 1 Introduction 195 2 Genetic Variation for Adult Preference and Larval Performance on Different Hosts in Drosophila tripunctata 196 3 Gene Flow Among Differentiated Populations 198 4 Genetic Correlations Between Preference and Performance 198 5 Density-Dependent Processes 200 6 Mutation-Selection Balance 201 7 Lack of Genetic Variation for Host Preference in Drosophila quinaria 203 8 Summary and Conclusions 204 References 205 Chapter 12 Theoretical Analysis of Habitat Selection and the Maintenance of Genetic Variation Philip W Hedrick 1 Introduction 209 2 Genotypic-Specific Habitat Selection 209 2 1 The General Model 209 2 2 Parameterization of Habitat Selection 211 2 3 Habitat Selection by Females 213 2 4 Hard or Soft Selection 214 2 5 Two Loci 214 3 Results 215 3 1 Parameterization of Habitat Selection 215 CONTENTS 3 2 Habitat Selection by Females 217 3 3 Hard or Soft Selection 218 3 4 Two Loci 218 4 Discussion 221 5 Summary 226 References 226 Biochemical Genetics: Introduction 229 Chapter 13 Alcohol Dehydrogenase and Alcohol Tolerance in Drosophila melanogaster Billy W Geer, Pieter W H Heinstra, Ann M Kapoun, and Aleid van der Zel 1 The Physiological Effects of Alcohols 231 1 1 The Toxic Effects of Alcohols at the Cellular Level 231 1 2 Changes in Lipid Composition Induced by Low Levels of Ethanol 233 2 The Metabolism of Alcohols 234 2 1 Metabolic Pathways that Degrade Ethanol 234 2 2 Metabolic Pathways that Degrade Ethanol-Derived Acetaldehyde 237 2 3 Catalytic properties of D melanogaster ADH 238 3 The Genetic Basis for Short Term Adaptation to Ethanol 240 3 1 Regulation of the Expression of the Adh Gene 240 3 2 DNA Sequences that Affect the Induction of Adh mRNA 242 3 3 Induction of mRNA in Adh2 243 4 The Genetic Basis for Long Term Adaptation 244 4 1 The Epistatic Interaction of Adh and Gpdh 244 4 2 Metabolic Flux, Lipid Composition and Ethanol Tolerance 7 245 5 Summary 247 References 248 Chapter 14 Environmental Modulation of Alpha-Glycerol-3-Phosphate Oxidase (GPO) Activity in Larvae of Drosophila melanogaster Stephen W McKechnie, Jennifer L Ross, and Kerrin L Turney 1 Introduction 2 Materials and Methods CONTENTS xi 2 1 Fly Maintenance 256 2 2 GPO Enzyme Activity Determination 256 2 3 Starvation Experiment • 257- 2 4 Fatty Acid Feeding • 257 2 5 Cold Exposure J 257 2 6 Sucrose/Ethanol - mRNA Experiment 258 2 7 RNA Extractions 258 2 8 Dot Blots 258 2 9 Wine Selection ; 259 3 Results 259 3 1 Induction as a Clue to the Role of GPO in Larvae 259 3 2 Dietary Sucrose/Ethanol and Gpo mRNA Levels 262 3 3 Wine Selection 263 4 Discussion 266 5 Summary 268 References 269 Chapter 15 Physiology, Biochemistry and Molecular Biology of the Est-6 Locus in Drosophila melanogaster Rollin C Richmond, Karen M Nielsen, James- P -Brady- and Elizabeth M Snella • ! 1 Introduction i;; • 273 « - * 2 EST 6 as a Model System for Studying Molecular Evolution - : 274 3 Variation at the Est-6 Locus in Natural and Laboratory Populations - 276 4 Biochemical Characterization of the Allozymes of EST 6 278 5 Physiological Role of EST 6 281 5 1 Developmental Profile 281 5 2 Tissue Distribution 281 5 3 Transfer to Females 283 5 4 Behavioral and Physiological Effects of EST 6 284 6 Conclusions 287 7 Summary 288 References 289 Chapter 16 Insecticide Resistance as a Model System for Studying Molecular Evolution Robyn J Russell, Mira M Dumancic, Geoffrey G Foster, Gaye L Weller, Marion J Healy, and John G Oakeshott 1 Introduction 2 Major Mechanisms of Insecticide Resistance 2 1 Decreased Penetration xiv CONTENTS 2 2 Target Site Modification 294 2 3 Increased Detoxication of Insecticides 295 3 Resistance to Organophosphate Insecticides 297 3 1 Mechanism of Action of Organophosphates 297 3 2 Target Site Resistance: Mutations in Acetylcholinesterase 298 3 3 Metabolic Resistance: Carboxylesterase Mutations 299 4 Organophosphate Resistance in Lucilia cuprina 301 4 1 The Rgp_7 Locus 302 4 2 The £_ Locus 305 4 3 Molecular Analysis of the and RmL Loci 307 5 Summary 309 References 310 Chapter 17 Temperature Effects on Epicuticular Hydrocarbons and Sexual Isolation in Drosophila mojavensis Therese Ann Markow, and Eric C Toolson 1 Introduction 315 2 Cuticular Hydrocarbons of Insects 317 3 Cuticular Hydrocarbons and Cactophilic Drosophila 318 3 1 Temperature Shifts and Hydrocarbon Profiles 319 3 2 Temperature Shifts and Sexual Isolation 321 3 3 Hydrocarbons and Sexual Isolation 321 4 Temperature Effects in the Laboratory as a Model System for Sexual Isolation in Drosophila 325 5 Model for Sexual Selection/Sexual Isolation in Drosophila mojavensis 329 6 Summary 329 References 330 Molecular Evolution: Introduction 333 Chapter 18 Gene and Genome Structure in Diptera: Comparative Molecular Analysis of an Eye Colour Gene in Three Species Abigail Elizur, Ygal Haupt, Richard G Tearle, and Antony J Howells 1 Introduction 337 2 Materials and Methods 338 2 1 Insects 338 2 2 Preparation of Insect DNA and Southern Analysis 338 2 3 Construction and Screening of Genomic DNA Libraries 339 CONTENTS *v 2 4 Subcloning into Plasmid Vectors 339 2 5 DNA Sequencing and Computer Analysis 339 3 Results 340 3 1 Structures of the scarlet and topaz Genes 340 3 2 Comparison of Exon Sequences 341 3 3 Comparison of Zntron Sequences 342 3 4 Repeated Sequence DNA in the scarlet and topaz Regions 342 3 5 Natural Mutation at the scarlet and topaz Loci 346 4 Discussion 350 5 Summary 355 References 356 Chapter 19 Regulatory Evolution of B-Carboxyl Esterases in Drosophila John G Oakeshott, Marion J Healy, and Anne Y Game 1 Introduction 359 2 Interspecific Variation in B-Carboxyl Esterases 361 2 1 Structural Properties 361 2 2 Regulatory Variation 363 2 3 The Melanogaster Group 365 2 4 The Obscura Group 369 2 5 The Repleta Group 371 2 6 The Virilis Group 373 3 EST6 Variation within D melanogaster 374 3 1 Structural Polymorphisms 374 3 2 Activity Variation 375 3 3 Molecular Bases of Activity Variation 376 4 Conclusions 379 5 Summary 382 References 383 Chapter 20 -— Molecular Isolation and Preliminary Characterisation of a Duplicated Esterase Locus in Drosophila buzzatii Peter East, Anne Graham, and Gillian Whitington 1 Introduction 389 2 Evidence for a S-esterase Duplication in the mulleri Subgroup 390 3 Developmental Studies of the B-esterases of D buzzatii 391 4 Molecular Cloning of the Duplication 393 5 Transcriptional Organisation 394 6 DNA Sequence Analyses 395 7 Sequence Comparisons 400 8 Summary 403 References 405 CONTENTS Chapter 21 The Evolution of Adh Expression in the Repleta Group of Drosophila David T Sullivan, Peter W Atkinson, Cynthia A Bayer, and Marilyn A Menotti-Raymond 1 Introduction 407 2 Adh Gene Duplication 407 3 Molecular Characterization of Adh Loci in the Repleta Group 410 4 Evolution of the Adh Locus 411 5 Expression of Adh Genes 411 6 Summary 417 References 418 Chapter 22 Evolutionary Relationships of Enzymes in the Genus Drosophila Glen E Collier, Ross J Maclntyre, and David Featherston 1 Introduction 419 2 Evolution of Specific Enzymes 419 2 1 Arginine Kinase 421 2 2 6-Phospho-Gluconate Dehydrogenase 423 3 The Evolution of Drosophila Species Groups Based on Phylogenies Constructed with Genetic Distances 426 3 1 Sources of Data 427 3 2 Genetic Distance Estimates 429 3 3 Construction of Phylogenetic Trees 429 3 4 Comparison of Phylogenetic Trees 429 3 5 Analysis of the Data 434 4 Prospectus 440 5 Summary 442 References 442 Chapter 23 Genetic Differentiation Among Island Populations and Species of Cactophilic Drosophila in the West Indies W B Heed, A Sanchez, R Armengol, and A Fontdevila 1 Introduction 447 2 Materials and Methods 449 2 1 Collections 449 2 2 Starch Gel Electrophoresis 450 2 3 Staining Methods 451 2 4 Other Enzymes Not Scored 453 2 5 Allozyme and Electromorph Homology 453 2 6 Analyses of Data 453 CONTENTS xvii 3 Results 454 3 1 Identification of Electromorphs and Allozymes 454 3 2 Apomorphic Electromorphs and Allozymes 462 3 3 Genetic Distance Coefficients 463 3 4 Wright s F-Statistics 465 3 5 Genetic Identity Coefficients 467 3 6 Distance Wagner Trees 469 3 7 Phylogenetic Analysis using Parsimony 473 3 8 Genetic Distance Dendrograms 474 4 Discussion 475 4 1 Previous Studies 475 4 2 Outgroup Determination 476 4 3 Sources of Error 477 4 4 Species Identification 479 4 5 Significance of Host Plant Selection 479 4 6 Geographic Genetics 481 5 Summary 484 References 486 Contributors 491 Author Index 495 Organism Index 503 Subject Index 509
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spellingShingle Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia]
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title Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia]
title_auth Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia]
title_exact_search Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia]
title_full Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia] ed. by J. S. F. Barker ...
title_fullStr Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia] ed. by J. S. F. Barker ...
title_full_unstemmed Ecological and evolutionary genetics of Drosophila [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia] ed. by J. S. F. Barker ...
title_short Ecological and evolutionary genetics of Drosophila
title_sort ecological and evolutionary genetics of drosophila proceedings of an international workshop on ecological and evolutionary genetics of drosophila held january 5 10 1989 in armidale australia
title_sub [proceedings of an International Workshop on Ecological and Evolutionary Genetics of Drosophila, held January 5 - 10, 1989, in Armidale, Australia]
topic Genetik (DE-588)4071711-2 gnd
Drosophila (DE-588)4150708-3 gnd
Evolution (DE-588)4071050-6 gnd
topic_facet Genetik
Drosophila
Evolution
Konferenzschrift 05.01.1989-10.01.1989 Armidale
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