Hybridoma technology in the biosciences and medicine
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| Format: | Buch |
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| Sprache: | English |
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New York [u.a.]
Plenum Press
1985
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| 245 | 1 | 0 | |a Hybridoma technology in the biosciences and medicine |c ed. by Timothy A. Springer |
| 264 | 1 | |a New York [u.a.] |b Plenum Press |c 1985 | |
| 300 | |a XXV, 602 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Anticorps monoclonaux | |
| 650 | 4 | |a Hybridomes | |
| 650 | 4 | |a Antibodies, Monoclonal | |
| 650 | 4 | |a Hybridomas | |
| 650 | 4 | |a Hybridomas |x immunology | |
| 650 | 4 | |a Immunologic Techniques | |
| 650 | 4 | |a Monoclonal antibodies | |
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Datensatz im Suchindex
| _version_ | 1804114914743156736 |
|---|---|
| adam_text | Hybridoma Technology
in the Biosciences
and Medicine
Edited by
TIMOTHY A SPRINGER
Dana-Farber Cancer Institute
Harvard Medical School
Boston, Massachusetts
Plenum Press • New York and London
Contents
Part I THE PRODUCTION AND IMPROVEMENT THROUGH GENETIC
ENGINEERING OF MONOCLONAL ANTIBODIES
1 Monoclonal Antibody Strategy and Techniques
Zelig Eshhar
1 Introduction 3 2 Factors That Control Immunogenicity 6
3 Strategy and Tactics for the Preparation of Monoclonal
Antibodies 9 4 Procedures for the Production of Monoclonal
Antibodies 11 5 Screening Assays for Monoclonal Antibody 27
References 36
2 Strategies for Production of Human Monoclonal Antibodies
Susan P C Cole, Danuta Kozbor, and John C C Roder
1 Introduction
Antibodies 43
Fusion Partners
Technique 48
Antibodies 49
43 2 Human versus Mouse Monoclonal
3 Epstein-Barr Virus 44 4 Human Hybridomas
45 5 Epstein-Barr Virus Hybridoma
6 Large-Scale Production of Human Monoclonal
7 Possible Limitations and Future
Developments 50 References 52
3 The Antibody Combining Site
Edgar Haber and firi Novotny
1 Introduction 57 2 Structure of the Antibody Combining Site 58
3 Digoxin-Specific Monoclonal Antibodies 64 4 A Look into the
Future 72 References 73
4 The Generation of Better Monoclonal Antibodies through Somatic
Mutation
Gad Spira, Antonio BargeJJesi, Roberta R Pollock, Hector L Aguila,
and Matthew D Scharff
1 Introduction 77 2 Class and Subclass Switch Variants 80
3 Deletion Mutants 83 4 Antigen Binding Mutants 85
5 Conclusion 86 References 86
xvii
xviii Contents
5 Switching the Isotype of Monoclonal Antibodies
Thomas J Kipps
1 Introduction 89 2 Mouse Immunoglobulin isotypes 89
3 The Genetics of Immunoglobulin Isotype Expression 90
4 Spontaneous Isotype Switching in Myelomas and Hybridomas 91
5 Techniques of Switch Variant Selection 92 6 Kinetics of Isotype
Switching 96 7 Effects of Mutagens on the Rate of Isotype
Switching 98 References 98
6 The Analysis of Structural Diversity in the Antibody Response
Gillian M Griffiths and Cesar Miistein
1 Introduction 103 2 RNA Sequencing 104 3 Analyzing
Immunoglobulin Diversity by mRNA Sequencing 104
4 Protocol 110 5 Analyzing the Structural Diversity of the
Antibody Response 112 References 115
7 Expressions of Cloned Immunoglobulin Genes
Frederick W Alt
1 Introduction 117 2 Mechanism of Immunoglobulin Gene
Assembly 117 3 Assembly of Transfected Genes 120 4 Control
of Immunoglobulin Gene Expression 123 5 Expression of
Introduced Genes 125 6 Transgenic Mice 128
7 Summary 130 References 130
Part II MONOCLONAL ANTIBODIES IN BIOMEDICAL RESEARCH:
PRINCIPLES AND APPLICATIONS
8 The Immunochemistry of Monoclonal Antibodies: Studies on One
Immunologic Supergene (HLA) with the Products of Another
Peter Parham
1 Introduction 137 2 Fragmentation of IgG to Give F(ab )2 and
Fab 138 3 Fab/c: A Univalent Fragment with Effector
Function 140 4 Isotype Switch Variants 142 5 Binding of
Monoclonal Antibodies to Cell Surface Antigens 144 6 Hybrid
Antibodies 147 7 Enhancing Effects of Monoclonal Antibodies in
Solution 149 8 Quantitative Serologic Analysis of HLA-A, B
Molecules 153 9 Epitope Mapping 155 References 159
Contents xix
9 Purification of Membrane Glycoproteins with Monoclonal Antibody
Affinity Columns
Pauline Johnson, AJan F Williams, and Gillian R Woollett
1 Introduction 163 2 Key Aspects in Monoclonal Antibody
Affinity Chromatography 164 3 Purifications Based on Antigenic
Determinants Stable to Denaturation 168 References 174
10 Transferrin Receptor
Ian S Trowbridge
1 Introduction 177 2 Structure of the Transferrin Receptor 178
3 Functional Aspects of the Transferrin Receptor 182 4 Gene
Transfer and Molecular Cloning of the Human Transferrin
Receptor 185 5 Concluding Remarks 186 References 187
11 Functional and Structural Interrelationships among the Mac-1, LFA-1
Family of Leukocyte Adhesion Glycoproteins, and their Deficiency in
a Novel Heritable Disease
Timothy A Springer and Donald C Anderson
1 Introduction 191 2 Probing Cell Surface Functions with
Monoclonal Antibodies 192 3 Functional Properties of LFA-1 193
4 Functional Properties of Mac-1, a Molecule Related to LFA-1 194
5 Glycoprotein Family Interrelationships 194 6 Mac-1, LFA-1
Deficiency-Disease 198 References 200
12 Demonstration of Receptor Function of Membrane Proteins by
Selection and Immobilization with Monoclonal Antibodies
Janis Jacks Weis and Douglas T Fearon
1 Background 207 2 Demonstration of Specificity of Monoclonal
Antibody-Immobilized Antigen for Particulate Ligand 212
3 Demonstration of Specificity of Monoclonal Antibody-Immobilized
Antigen for Soluble Ligand 216 4 Summary 218
References 218
XX Contents
13 Production of Rat x Mouse Hybridomas for the Study of the Nicotinic
Acetylcholine Receptor
Susan M Hochschwender, Lorene K Langeberg, DougJas W Schneider,
and Jon M Lindstrom
1 Introduction 223 2 Production of Rat Monoclonal
Antibodies 224 3 Characterization of Monoclonal Antibodies and
Specificity Determination 230 4 Use of Monoclonal Antibodies to
Study Acetylcholine Receptors 232 5 Use of Monoclonal
Antibodies to Study Myasthenia Gravis and Experimental Autoimmune
Myasthenia Gravis 234 References 234
14 Sperm Surface Domains
Diana G Myles and Paul Primakoff
1 Introduction 239 2 Production and Characterization of the
Monoclonal Antibodies 240 3 Topographic Localization of Surface
Antigens: A Map of the Guinea Pig Sperm Cell Surface 242 4 How
Do Surface Antigens Become Localized during Differentiation of the
Sperm Cell? 244 5 What Keeps Antigens in Their Domains? The
Maintenance of Surface Topography 245 6 Dynamics of Surface
Localization: The Migration of Surface Molecules from Their Original
Domain to a New Domain 246 7 Conclusion 249
References 249
15 The Cytoskeleton
Lan Bo Chen, Sharon Rosenberg, Karen K Nadakavukaren, Elizabeth
S Walker, Elizabeth L Shepard, and Glenn D Steele, Jr
1 Introduction 251 2 Experimental Procedures 252
3 Examples of Monoclonal Antibodies Specific for Cytoskeletal
Components 255 4 Examples of Utility of Monoclonal Antibodies
against Cytoskeleton 264 5 Concluding Remarks 265
References 267
16 Monoclonal Antibodies as Molecular Probes of the Nervous System
Colin J Barnstable
1 Introduction 269 2 Immunologic Structure of the Retina 272
3 In Vitro Studies of Retinal Cells 281 4 Layers, Gradients, and
Positional Information 285 5 Discussion 287 References 287
Contents xxi
17 Application of Monoclonal Antibodies to Issues of Plasma Lipoprotein
Structure and Function
Linda K Curtiss
1 Introduction 291 2 Background Plasma Lipoprotein Structure
and Function 291 3 Monoclonal Antibodies That Bind
Lipoproteins 293 4 Application of Monoclonal Antibodies to
Issues of Lipoprotein Structure and Function 297 5 Future
Applications and Summary 304 References 305
18 Enzyme Active Sites
Sandra J Smith-Gill
1 Introduction 309 2 Enzymes with Multiple Interacting
Sites 313 3 Molecular Mechanisms of Catalysis 320
4 Conclusions 323 References 324
19 Monoclonal Antibodies in Studies of the Hong Kong Influenza
Hemagglutinin
J ) Skehel and Donald C Wiley
1 Introduction 327 2 The X-31 Hong Kong Hemagglutinin 327
3 Antibody Binding Sites 328 4 Antigenic Variation 333
5 Analysis of Hemagglutinin Functions 336 References 338
20 Virus Receptors on Somatic and Immune Cells
Man Sung Co, Glen N Gaulton, Noboru Matsuzaki, and Mark I Greene
1 Introduction 341 2 Reovirus as a Model System for Studying
Viral Receptor Interactions 342 3 Studies of Reovirus Receptor
with Anti-Idiotypic Antibody 343 4 Similarities of Somatic and
Immune Cell Viral Receptors 347 5 Conclusions 349
References 349
Part III USES OF ANTIBODIES IN RECOMBINANT DNA TECHNOLOGY
21 Designing and Using Site-Specific Antibodies to Synthetic Peptides
Brian S Schaffhausen
1 Introduction 355 2 How Can Antibodies to Synthetic Peptides
Be Used? 357 3 Preparation of Antibodies to Synthetic
Peptides 361 4 A Specific Example of the Use of Antipeptide
Antibody: Polyoma Middle T Antigen 366 Conclusion 367
References 368
xxii Contents
22 Gene Transfer as a General Approach to Facilitate the Recombinant
DNA Cloning of Surface Antigen Genes
Frank H Ruddle
1 Introduction 375 2 The Transferrin Receptor System 375
3 General Strategy for Gene Cloning 375 4 Isolation of Primary
Transformants 378 5 Isolation of Secondary Transformants 379
6 Recombinant DNA Cloning of Secondary Transformant DNA and
Validation of Gene Cloning 379 7 Discussion and Summary 380
References 381
23 Amplification and Molecular Cloning of Transfected Genes
Paula Kavathas
1 Introduction 383 2 Movement of DNA from Outside the Cell
into the Nucleus: Basic Process 384 3 Methods for Transferring
DNA into Cells 384 4 Methods of Selection 385 5 Cloning
Genes 386 6 Amplification 390 7 Analyzing Cell Surface
Antigens 392 References 393
24 Screening Xgtll Expression Libraries with Antibody Probes
Michael Snyder and Ronald W Davis
1 Introduction 397 2 gtll 397 3 Escherichia coli Host 399
4 Constructing X gtll Recombinant DNA Libraries 399
5 Preparation of Antibody Probes 401 6 Screening Expression
Vector Libraries with Antibody Probes 402 7 Confirmation of Gene
Identity 404 8 Conclusion 405 References 406
25 Purification of LowzAbundance Messenger RNAs by Polysome
Isolation with Polyclonal~~Antibodies
Jan P Kraus
1 Introduction 407 2 Materials and Methods 409 3 Results
and Discussion 412 4 Conclusions 414 References 415
26 Purification of Messenger RNA by Polysome Isolation with
Monoclonal Antibodies
Joseph P Brown, Timothy M Rose, and Gregory D Plowman
1 Introduction 419 2 Monoclonal Antibodies 419 3 Assaying
mRNA Activity 421 4 Polysome Immunopurification 422
5 cDNA Cloning 423 6 Characterization of cDNA Clones 423
7 Discussion 424 References 425
Contents xxiii
27 Expression of Foreign Proteins on the Surface of Xenopus laevis
Oocytes
Jay C L/nkeJess, Daniel A Portnoy, Andrew L Luster, and Ellen Pure
1 Introduction 427 2 Methods 429 3 Results and
Discussion 431 4 Concluding Remarks 435 References 436
Part IV MONOCLONAL ANTIBODIES IN DIAGNOSIS AND THERAPY
28 Antibodies in Vivo
EdgarHaber
1 Introduction 441 2 Antibodies to the p-Adrenergic
Receptor 442 3 Renin-Specific Antibodies 444 4 Digoxin-
Specific Antibody in the Clinical Reversal of Toxicity 444
5 Imaging of Myocardial Infarcts 448 6 Detecting Thrombi in
Vivo 449 7 How Will Antibodies Be Established as Practical
Diagnostic and Therapeutic Agents? 450 8 Concluding
Comment 451 References 451
29 Human Complement-Fixing Antibodies in Transplantation
G Hale and H Waldmann
1 Introduction 453 2 Possible Reasons for Poor Lytic Ability of
Monoclonal Antibodies 454 3 Monoclonal Antibodies with Good
Lytic Ability 455 4 Therapeutic Applications 456
5 Techniques for Enhancing Complement-Mediated Lysis 461
6 Conclusion 465 References 466
30 Manipulation of T-Cell Populations to Abrogate Allograph Rejection
Charles_B Carpenter
1 Introduction 473 2 The Nature of Allograft Rejection 473
3 Monoclonal Anti-T-Cell Antibodies 479 4 Pan-T-Cell
Monoclonal Antibodies in Renal Transplantation 484
5 Immunogenicity of Mouse Immunoglobulins 488
6 Conclusion 489 References 490
31 Serotherapy and Bone Marrow Transplantation
Jerome Ritz, Stephen E Sallan, Robert C Bast, Jr , Tak Takvorian, and
Stuart F Schlossman
1 Introduction 493 2 Acute Lymphoblastic Leukemic Antigens:
Targets for Immunotherapy 494 3 Results 496
4 Discussion 500 References 502
xxiv Contents
Part V T-CELL CLONES AND THEIR PRODUCTS
32 The Murine T-Cell Antigen:la Receptor: Functional and Structural
Analysis with a Monoclonal Antireceptor Antibody
Jonathan Kaye
1 Introduction 507 2 Approaches to Isolating the T-Cell
Antigen:MHC Receptor 508 3 The Production of Antireceptor
Antibodies against Murine Helper T-Cell Lines 509 4 The
Biological Activity of a Monoclonal Antireceptor Antibody 513
5 Structural Analysis of the T-Cell Antigen:MHC Receptor 520
6 Conclusions 523 References 523
33 Functional T-Cell Hybridomas
Kenneth L Rock
1 Introduction 527 2 Methodology 527 3 Applications 536
References 541
34 The Mechanisms That Control the Proliferation and Activity of
Cytolytic T Lymphocytes
Markus Nabholz
1 Introduction 545 2 The Control of Lymphocyte
Proliferation 546 3 The Control of the Expression of CTL-Effector
Function 551- 4—Concluding Remarks on the Role of T-Cell
Hybrids 554 References 554
35 Human Cytolytic T-Lymphocyte Clones and Their Function-Associated
Cell Surface Molecules
Alan M Krensky, Steven J Mentzer, Julia L Greenstein, Mary
Crimmins, Carol Clayberger, Timothy A Springer, and Steven J
Burakoff
1 Introduction 559 2 Methods 560 3 Results and
Discussion 561 4 Summary 567 5 Conclusion 569
References 569
Contents XXV
36 The Use of Mouse Lymphocyte Clones to Define T-Cell Gene Products
and Analyze Lymphocyte Gene Activation
Gary J Nabel
1 Introduction 575 2 The Generation of T-Cell Clones 575
3 Definition of a T-Cell Gene Product in a T-Cell Clone 578
4 Experimental Procedures 581 References 584
37 Interleukin 1, Interferon Gamma, and the Modulation of Macrophage-
T Cell Interactions
David I Beller
1 Introduction: Antigen Presentation 587 2 Interleukin 1 588
3 Interferon Gamma 590 4 Summary 594 References 594
Index 597
|
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV000461540 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T15:14:17Z |
| institution | BVB |
| isbn | 0306419963 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-000284199 |
| oclc_num | 12134761 |
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| physical | XXV, 602 S. Ill., graph. Darst. |
| publishDate | 1985 |
| publishDateSearch | 1985 |
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| publisher | Plenum Press |
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| spelling | Hybridoma technology in the biosciences and medicine ed. by Timothy A. Springer New York [u.a.] Plenum Press 1985 XXV, 602 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Anticorps monoclonaux Hybridomes Antibodies, Monoclonal Hybridomas Hybridomas immunology Immunologic Techniques Monoclonal antibodies Monoklonaler Antikörper (DE-588)4040094-3 gnd rswk-swf Hybridom (DE-588)4160843-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Monoklonaler Antikörper (DE-588)4040094-3 s Hybridom (DE-588)4160843-4 s DE-604 Springer, Timothy A. 1948- Sonstige (DE-588)1029978263 oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000284199&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hybridoma technology in the biosciences and medicine Anticorps monoclonaux Hybridomes Antibodies, Monoclonal Hybridomas Hybridomas immunology Immunologic Techniques Monoclonal antibodies Monoklonaler Antikörper (DE-588)4040094-3 gnd Hybridom (DE-588)4160843-4 gnd |
| subject_GND | (DE-588)4040094-3 (DE-588)4160843-4 (DE-588)4143413-4 |
| title | Hybridoma technology in the biosciences and medicine |
| title_auth | Hybridoma technology in the biosciences and medicine |
| title_exact_search | Hybridoma technology in the biosciences and medicine |
| title_full | Hybridoma technology in the biosciences and medicine ed. by Timothy A. Springer |
| title_fullStr | Hybridoma technology in the biosciences and medicine ed. by Timothy A. Springer |
| title_full_unstemmed | Hybridoma technology in the biosciences and medicine ed. by Timothy A. Springer |
| title_short | Hybridoma technology in the biosciences and medicine |
| title_sort | hybridoma technology in the biosciences and medicine |
| topic | Anticorps monoclonaux Hybridomes Antibodies, Monoclonal Hybridomas Hybridomas immunology Immunologic Techniques Monoclonal antibodies Monoklonaler Antikörper (DE-588)4040094-3 gnd Hybridom (DE-588)4160843-4 gnd |
| topic_facet | Anticorps monoclonaux Hybridomes Antibodies, Monoclonal Hybridomas Hybridomas immunology Immunologic Techniques Monoclonal antibodies Monoklonaler Antikörper Hybridom Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=000284199&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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