The impact of Helicobacter pylori infection, eradication therapy, and probiotics intervention on gastric microbiota in young adults

Background The impact of probiotics on non‐Helicobacter pylori gastric microbiota and its role in microbial restoration after eradication were relatively unknown. We aimed to explore the effect of H. pylori eradication and probiotic intervention on gastric microbiota in young adults. Methods Fifty‐s...

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Veröffentlicht in:Helicobacter (Cambridge, Mass.) Mass.), 2021-12, Vol.26 (6), p.e12848-n/a, Article 12848
Hauptverfasser: Yuan, Ziying, Xiao, Shiyu, Li, Sizhu, Suo, Baojun, Wang, Ye, Meng, Lingmei, Liu, Zuojing, Yin, Zhihao, Xue, Yan, Zhou, Liya
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container_issue 6
container_start_page e12848
container_title Helicobacter (Cambridge, Mass.)
container_volume 26
creator Yuan, Ziying
Xiao, Shiyu
Li, Sizhu
Suo, Baojun
Wang, Ye
Meng, Lingmei
Liu, Zuojing
Yin, Zhihao
Xue, Yan
Zhou, Liya
description Background The impact of probiotics on non‐Helicobacter pylori gastric microbiota and its role in microbial restoration after eradication were relatively unknown. We aimed to explore the effect of H. pylori eradication and probiotic intervention on gastric microbiota in young adults. Methods Fifty‐six H. pylori‐negative and 95 H. pylori‐positive subjects aged 19–30 were included in this study. H. pylori‐infected individuals were randomly assigned to quadruple therapy, probiotics supplemented quadruple therapy, or probiotics monotherapy group. Gastric mucosa and gastric juice samples were collected before and 2 months after treatment for 16SrRNA gene sequencing. Results The gastric microbial community structure and composition differed from H. pylori‐negative subjects 2 months after successful H. pylori eradication. The α diversity of gastric mucosal microbiota significantly increased and was higher than H. pylori‐negative subjects, while the α diversity of gastric juice microbiota decreased and was lower than the H. pylori‐negative. After probiotics supplemented eradication treatment, Bifidobacterium was enriched in gastric mucosa, Lactobacillus was enriched in gastric juice, potentially pathogenic bacteria such as Fusobacterium and Campylobacter decreased, and the microbial diversity was closer to that of H. pylori‐negative subjects compared to quadruple therapy group. Probiotics monotherapy significantly altered the diversity, community structure, and composition of gastric microbiota but showed no advantage in H. pylori inhibition and upregulating beneficial bacteria such as Bifidobacterium and Lactobacillus and related metabolism pathways. Certain potentially pathogenic bacteria such as Fusobacterium increased after probiotic monotherapy. Conclusion H. pylori eradication significantly disrupted gastric microbiota in young adults and could not be restored in a short time. Probiotics supplementation partially helped restore the gastric dysbiosis caused by eradication therapy, but it might be unnecessary for H. pylori‐infected young adults to take probiotics alone.
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We aimed to explore the effect of H. pylori eradication and probiotic intervention on gastric microbiota in young adults. Methods Fifty‐six H. pylori‐negative and 95 H. pylori‐positive subjects aged 19–30 were included in this study. H. pylori‐infected individuals were randomly assigned to quadruple therapy, probiotics supplemented quadruple therapy, or probiotics monotherapy group. Gastric mucosa and gastric juice samples were collected before and 2 months after treatment for 16SrRNA gene sequencing. Results The gastric microbial community structure and composition differed from H. pylori‐negative subjects 2 months after successful H. pylori eradication. The α diversity of gastric mucosal microbiota significantly increased and was higher than H. pylori‐negative subjects, while the α diversity of gastric juice microbiota decreased and was lower than the H. pylori‐negative. After probiotics supplemented eradication treatment, Bifidobacterium was enriched in gastric mucosa, Lactobacillus was enriched in gastric juice, potentially pathogenic bacteria such as Fusobacterium and Campylobacter decreased, and the microbial diversity was closer to that of H. pylori‐negative subjects compared to quadruple therapy group. Probiotics monotherapy significantly altered the diversity, community structure, and composition of gastric microbiota but showed no advantage in H. pylori inhibition and upregulating beneficial bacteria such as Bifidobacterium and Lactobacillus and related metabolism pathways. Certain potentially pathogenic bacteria such as Fusobacterium increased after probiotic monotherapy. Conclusion H. pylori eradication significantly disrupted gastric microbiota in young adults and could not be restored in a short time. Probiotics supplementation partially helped restore the gastric dysbiosis caused by eradication therapy, but it might be unnecessary for H. pylori‐infected young adults to take probiotics alone.</description><identifier>ISSN: 1083-4389</identifier><identifier>EISSN: 1523-5378</identifier><identifier>DOI: 10.1111/hel.12848</identifier><identifier>PMID: 34448282</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Adults ; Anti-Bacterial Agents - therapeutic use ; Bacteria ; Bifidobacterium ; Community structure ; Composition ; Dietary supplements ; Dysbacteriosis ; Fusobacterium ; Gastric juice ; gastric microbiota ; Gastric mucosa ; Gastroenterology &amp; Hepatology ; Gene sequencing ; H. pylori eradication ; Helicobacter Infections - drug therapy ; Helicobacter pylori ; Humans ; Juices ; Lactobacillus ; Life Sciences &amp; Biomedicine ; Metabolism ; Microbiology ; Microbiota ; Microorganisms ; Probiotics ; RNA, Ribosomal, 16S ; Science &amp; Technology ; Therapy ; Young Adult ; Young adults</subject><ispartof>Helicobacter (Cambridge, Mass.), 2021-12, Vol.26 (6), p.e12848-n/a, Article 12848</ispartof><rights>2021 John Wiley &amp; Sons Ltd</rights><rights>2021 John Wiley &amp; Sons Ltd.</rights><rights>Copyright © 2021 John Wiley &amp; Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>43</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000689491800001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3538-354df3596bc124fab080e069ccbd69231203b74ed587f370e3a4cccc4c3530123</citedby><cites>FETCH-LOGICAL-c3538-354df3596bc124fab080e069ccbd69231203b74ed587f370e3a4cccc4c3530123</cites><orcidid>0000-0002-2064-5522 ; 0000-0002-4611-1457 ; 0000-0001-6456-3994 ; 0000-0001-5899-8705</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fhel.12848$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fhel.12848$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,39265,45581,45582</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34448282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Ziying</creatorcontrib><creatorcontrib>Xiao, Shiyu</creatorcontrib><creatorcontrib>Li, Sizhu</creatorcontrib><creatorcontrib>Suo, Baojun</creatorcontrib><creatorcontrib>Wang, Ye</creatorcontrib><creatorcontrib>Meng, Lingmei</creatorcontrib><creatorcontrib>Liu, Zuojing</creatorcontrib><creatorcontrib>Yin, Zhihao</creatorcontrib><creatorcontrib>Xue, Yan</creatorcontrib><creatorcontrib>Zhou, Liya</creatorcontrib><title>The impact of Helicobacter pylori infection, eradication therapy, and probiotics intervention on gastric microbiota in young adults</title><title>Helicobacter (Cambridge, Mass.)</title><addtitle>HELICOBACTER</addtitle><addtitle>Helicobacter</addtitle><description>Background The impact of probiotics on non‐Helicobacter pylori gastric microbiota and its role in microbial restoration after eradication were relatively unknown. We aimed to explore the effect of H. pylori eradication and probiotic intervention on gastric microbiota in young adults. Methods Fifty‐six H. pylori‐negative and 95 H. pylori‐positive subjects aged 19–30 were included in this study. H. pylori‐infected individuals were randomly assigned to quadruple therapy, probiotics supplemented quadruple therapy, or probiotics monotherapy group. Gastric mucosa and gastric juice samples were collected before and 2 months after treatment for 16SrRNA gene sequencing. Results The gastric microbial community structure and composition differed from H. pylori‐negative subjects 2 months after successful H. pylori eradication. The α diversity of gastric mucosal microbiota significantly increased and was higher than H. pylori‐negative subjects, while the α diversity of gastric juice microbiota decreased and was lower than the H. pylori‐negative. After probiotics supplemented eradication treatment, Bifidobacterium was enriched in gastric mucosa, Lactobacillus was enriched in gastric juice, potentially pathogenic bacteria such as Fusobacterium and Campylobacter decreased, and the microbial diversity was closer to that of H. pylori‐negative subjects compared to quadruple therapy group. Probiotics monotherapy significantly altered the diversity, community structure, and composition of gastric microbiota but showed no advantage in H. pylori inhibition and upregulating beneficial bacteria such as Bifidobacterium and Lactobacillus and related metabolism pathways. Certain potentially pathogenic bacteria such as Fusobacterium increased after probiotic monotherapy. Conclusion H. pylori eradication significantly disrupted gastric microbiota in young adults and could not be restored in a short time. Probiotics supplementation partially helped restore the gastric dysbiosis caused by eradication therapy, but it might be unnecessary for H. pylori‐infected young adults to take probiotics alone.</description><subject>Adults</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Bacteria</subject><subject>Bifidobacterium</subject><subject>Community structure</subject><subject>Composition</subject><subject>Dietary supplements</subject><subject>Dysbacteriosis</subject><subject>Fusobacterium</subject><subject>Gastric juice</subject><subject>gastric microbiota</subject><subject>Gastric mucosa</subject><subject>Gastroenterology &amp; Hepatology</subject><subject>Gene sequencing</subject><subject>H. pylori eradication</subject><subject>Helicobacter Infections - drug therapy</subject><subject>Helicobacter pylori</subject><subject>Humans</subject><subject>Juices</subject><subject>Lactobacillus</subject><subject>Life Sciences &amp; Biomedicine</subject><subject>Metabolism</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Probiotics</subject><subject>RNA, Ribosomal, 16S</subject><subject>Science &amp; 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Biomedicine</topic><topic>Metabolism</topic><topic>Microbiology</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Probiotics</topic><topic>RNA, Ribosomal, 16S</topic><topic>Science &amp; Technology</topic><topic>Therapy</topic><topic>Young Adult</topic><topic>Young adults</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Ziying</creatorcontrib><creatorcontrib>Xiao, Shiyu</creatorcontrib><creatorcontrib>Li, Sizhu</creatorcontrib><creatorcontrib>Suo, Baojun</creatorcontrib><creatorcontrib>Wang, Ye</creatorcontrib><creatorcontrib>Meng, Lingmei</creatorcontrib><creatorcontrib>Liu, Zuojing</creatorcontrib><creatorcontrib>Yin, Zhihao</creatorcontrib><creatorcontrib>Xue, Yan</creatorcontrib><creatorcontrib>Zhou, Liya</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Helicobacter (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Ziying</au><au>Xiao, Shiyu</au><au>Li, Sizhu</au><au>Suo, Baojun</au><au>Wang, Ye</au><au>Meng, Lingmei</au><au>Liu, Zuojing</au><au>Yin, Zhihao</au><au>Xue, Yan</au><au>Zhou, Liya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The impact of Helicobacter pylori infection, eradication therapy, and probiotics intervention on gastric microbiota in young adults</atitle><jtitle>Helicobacter (Cambridge, Mass.)</jtitle><stitle>HELICOBACTER</stitle><addtitle>Helicobacter</addtitle><date>2021-12</date><risdate>2021</risdate><volume>26</volume><issue>6</issue><spage>e12848</spage><epage>n/a</epage><pages>e12848-n/a</pages><artnum>12848</artnum><issn>1083-4389</issn><eissn>1523-5378</eissn><abstract>Background The impact of probiotics on non‐Helicobacter pylori gastric microbiota and its role in microbial restoration after eradication were relatively unknown. We aimed to explore the effect of H. pylori eradication and probiotic intervention on gastric microbiota in young adults. Methods Fifty‐six H. pylori‐negative and 95 H. pylori‐positive subjects aged 19–30 were included in this study. H. pylori‐infected individuals were randomly assigned to quadruple therapy, probiotics supplemented quadruple therapy, or probiotics monotherapy group. Gastric mucosa and gastric juice samples were collected before and 2 months after treatment for 16SrRNA gene sequencing. Results The gastric microbial community structure and composition differed from H. pylori‐negative subjects 2 months after successful H. pylori eradication. The α diversity of gastric mucosal microbiota significantly increased and was higher than H. pylori‐negative subjects, while the α diversity of gastric juice microbiota decreased and was lower than the H. pylori‐negative. After probiotics supplemented eradication treatment, Bifidobacterium was enriched in gastric mucosa, Lactobacillus was enriched in gastric juice, potentially pathogenic bacteria such as Fusobacterium and Campylobacter decreased, and the microbial diversity was closer to that of H. pylori‐negative subjects compared to quadruple therapy group. Probiotics monotherapy significantly altered the diversity, community structure, and composition of gastric microbiota but showed no advantage in H. pylori inhibition and upregulating beneficial bacteria such as Bifidobacterium and Lactobacillus and related metabolism pathways. Certain potentially pathogenic bacteria such as Fusobacterium increased after probiotic monotherapy. Conclusion H. pylori eradication significantly disrupted gastric microbiota in young adults and could not be restored in a short time. Probiotics supplementation partially helped restore the gastric dysbiosis caused by eradication therapy, but it might be unnecessary for H. pylori‐infected young adults to take probiotics alone.</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>34448282</pmid><doi>10.1111/hel.12848</doi><tpages>26</tpages><orcidid>https://orcid.org/0000-0002-2064-5522</orcidid><orcidid>https://orcid.org/0000-0002-4611-1457</orcidid><orcidid>https://orcid.org/0000-0001-6456-3994</orcidid><orcidid>https://orcid.org/0000-0001-5899-8705</orcidid></addata></record>
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subjects Adults
Anti-Bacterial Agents - therapeutic use
Bacteria
Bifidobacterium
Community structure
Composition
Dietary supplements
Dysbacteriosis
Fusobacterium
Gastric juice
gastric microbiota
Gastric mucosa
Gastroenterology & Hepatology
Gene sequencing
H. pylori eradication
Helicobacter Infections - drug therapy
Helicobacter pylori
Humans
Juices
Lactobacillus
Life Sciences & Biomedicine
Metabolism
Microbiology
Microbiota
Microorganisms
Probiotics
RNA, Ribosomal, 16S
Science & Technology
Therapy
Young Adult
Young adults
title The impact of Helicobacter pylori infection, eradication therapy, and probiotics intervention on gastric microbiota in young adults
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