ETRAP (efficient trapping and purification) of target protein polyclonal antibodies from GST–protein immune sera1

Recombinant GST (glutathione transferase) proteins are widely used as immunogens to generate polyclonal antibodies. Advantages of using GST proteins include: commercially available cloning vectors, vast literature for protein expression in Escherichia coli, the ease of protein purification, immunoge...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2010-12, Vol.57 (4), p.127-138
Hauptverfasser:	Crimmins, Dan L., Brada, Nancy A., Lockwood, Christina M., Griest, Terry A., Waldemer, Rachel J., Cervinski, Mark A., Ohlendorf, Matthew F., McQuillan, Jay J., Ladenson, Jack H.
Format:	Artikel
Sprache:	eng
Schlagworte:	epitope mapping GST–recombinant protein immunogens one‐step affinity chromatography
Online-Zugang:	Volltext
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