Characterization of G3BPs: Tissue specific expression, chromosomal localisation and rasGAP120 binding studies

Characterization of G3BPs: Tissue specific expression, chromosomal localisation and rasGAP120 binding studies

The G3BP (ras‐GTPase‐Activating Protein SH3‐Domain‐Binding Protein) family of proteins has been implicated in both signal transduction and RNA‐metabolism. We have previously identified human G3BP‐1, G3BP‐2, and mouse G3BP‐2. Here, we report the cloning of mouse G3BP‐1, the discovery of two alternati...

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Veröffentlicht in:Journal of cellular biochemistry 2002, Vol.84 (1), p.173-187
Hauptverfasser: Kennedy, Derek, French, Juliet, Guitard, Estelle, Ru, Kelin, Tocque, Bruno, Mattick, John
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RNA binding protein
RRM
SH3
signal transduction
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container_end_page 187
container_issue 1
container_start_page 173
container_title Journal of cellular biochemistry
container_volume 84
creator Kennedy, Derek
French, Juliet
Guitard, Estelle
Ru, Kelin
Tocque, Bruno
Mattick, John
description The G3BP (ras‐GTPase‐Activating Protein SH3‐Domain‐Binding Protein) family of proteins has been implicated in both signal transduction and RNA‐metabolism. We have previously identified human G3BP‐1, G3BP‐2, and mouse G3BP‐2. Here, we report the cloning of mouse G3BP‐1, the discovery of two alternatively spliced isoforms of mouse, and human G3BP‐2 (G3BP‐2a and G3BP‐2b), and the chromosomal localisation of human G3BP‐1 and G3BP‐2, which map to 5q14.2‐5q33.3 and 4q12‐4q24 respectively. We mapped the rasGAP120 interactive region of the G3BP‐2 isoforms and show that both G3BP‐2a and G3BP‐2b use an N‐terminal NTF2‐like domain for rasGAP120 binding rather than several available proline‐rich (PxxP) motifs found in members of the G3BPs. Furthermore, we have characterized the protein expression of both G3BP‐1 and G3BP‐2a/b in adult mouse tissues, and show them to be both tissue and isoform specific. J. Cell. Biochem. 84: 173–187, 2002. © 2001 Wiley‐Liss, Inc.
doi_str_mv 10.1002/jcb.1277
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subjects RNA binding protein
RRM
SH3
signal transduction
title Characterization of G3BPs: Tissue specific expression, chromosomal localisation and rasGAP120 binding studies
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