Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry

Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry

The plasma desorption mass spectra of N‐acetylated‐N,O‐permethylated peptides contain sufficient fragment ions to allow partial or complete sequence determination. By optimization of the derivatization procedure and an inclusion of a purification step by high‐performance liquid chromatrography (HPLC...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biological Mass Spectrometry 1990-10, Vol.19 (10), p.589-596
Hauptverfasser: Talbo, Gert, Roepstorff, Peter
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Mass Spectrometry - methods
Molecular Sequence Data
Peptides - genetics
Sequence Alignment - methods
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 596
container_issue 10
container_start_page 589
container_title Biological Mass Spectrometry
container_volume 19
creator Talbo, Gert
Roepstorff, Peter
description The plasma desorption mass spectra of N‐acetylated‐N,O‐permethylated peptides contain sufficient fragment ions to allow partial or complete sequence determination. By optimization of the derivatization procedure and an inclusion of a purification step by high‐performance liquid chromatrography (HPLC) overall sensitivities on the high picomole level are obtained. By using limiting derivatization conditions the fully derivatized peptide is easily selected from the HPLC separation. Mainly N‐terminal sequence ions are observed, facilitating sequence determination of naturally N‐blocked peptides. Complete sequence determination of naturally formylated gramicidin A containing 15 amino acid residues and the naturally N‐acetylated N‐terminal heptapeptide from an acyl‐CoA binding protein is demonstrated. The sequence of the first six N‐terminal residues was obtained by derivatization of the A‐chain of insulin.
doi_str_mv 10.1002/bms.1200191003
format Article
fullrecord <record><control><sourceid>istex_pubme</sourceid><recordid>TN_cdi_wiley_primary_10_1002_bms_1200191003_BMS1200191003</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_WNG_QL91TKCZ_K</sourcerecordid><originalsourceid>FETCH-LOGICAL-i3243-9f9ecc35ba9a55206b21f1210c96dd5e10c570bf7859d7cf248561fe3514a22e3</originalsourceid><addsrcrecordid>eNpFkF1PwjAUhhujQUVvvTPZD7DYD7q1l0oUDQgxYEy8abrtLEw3NtcS3b-3OAJX5-M9z5ucF6ErSgaUEHYbl3ZAGSFU-ZEfoTNKVIiVlPJ42wuGFSfhKTq39tMfSH_VQz3GpJBMnKHVAr43sE4gSMFBU-Zr4_JqHVRZMMMmAdcWxkGKZzdzXHsd3KrbBDXULk_BBnEb1IWxpfEWtmrqf7401ga2hsQ1lYea9gKdZKawcLmrffT2-LAcPeHpfPw8upvinLMhxypTkCRcxEYZIRgJY0YzyihJVJimAnwjIhJnkRQqjZKMDaUIaQZc0KFhDHgfXXe-9SYuIdV1k5emafXuY6-rTv_JC2j3MiV6G6f2cepDnPr-ZXGYPIs7NrcOfvesab50GPFI6PfZWL9OFV1ORh96wv8A5Lt5zg</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry</title><source>MEDLINE</source><source>Wiley Online Library Journals</source><source>Alma/SFX Local Collection</source><creator>Talbo, Gert ; Roepstorff, Peter</creator><creatorcontrib>Talbo, Gert ; Roepstorff, Peter</creatorcontrib><description>The plasma desorption mass spectra of N‐acetylated‐N,O‐permethylated peptides contain sufficient fragment ions to allow partial or complete sequence determination. By optimization of the derivatization procedure and an inclusion of a purification step by high‐performance liquid chromatrography (HPLC) overall sensitivities on the high picomole level are obtained. By using limiting derivatization conditions the fully derivatized peptide is easily selected from the HPLC separation. Mainly N‐terminal sequence ions are observed, facilitating sequence determination of naturally N‐blocked peptides. Complete sequence determination of naturally formylated gramicidin A containing 15 amino acid residues and the naturally N‐acetylated N‐terminal heptapeptide from an acyl‐CoA binding protein is demonstrated. The sequence of the first six N‐terminal residues was obtained by derivatization of the A‐chain of insulin.</description><identifier>ISSN: 1052-9306</identifier><identifier>ISSN: 0887-6134</identifier><identifier>EISSN: 1096-9888</identifier><identifier>DOI: 10.1002/bms.1200191003</identifier><identifier>PMID: 2285825</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>Amino Acid Sequence ; Mass Spectrometry - methods ; Molecular Sequence Data ; Peptides - genetics ; Sequence Alignment - methods</subject><ispartof>Biological Mass Spectrometry, 1990-10, Vol.19 (10), p.589-596</ispartof><rights>Copyright © 1990 John Wiley &amp; Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbms.1200191003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbms.1200191003$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2285825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Talbo, Gert</creatorcontrib><creatorcontrib>Roepstorff, Peter</creatorcontrib><title>Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry</title><title>Biological Mass Spectrometry</title><addtitle>Biol. Mass Spectrom</addtitle><description>The plasma desorption mass spectra of N‐acetylated‐N,O‐permethylated peptides contain sufficient fragment ions to allow partial or complete sequence determination. By optimization of the derivatization procedure and an inclusion of a purification step by high‐performance liquid chromatrography (HPLC) overall sensitivities on the high picomole level are obtained. By using limiting derivatization conditions the fully derivatized peptide is easily selected from the HPLC separation. Mainly N‐terminal sequence ions are observed, facilitating sequence determination of naturally N‐blocked peptides. Complete sequence determination of naturally formylated gramicidin A containing 15 amino acid residues and the naturally N‐acetylated N‐terminal heptapeptide from an acyl‐CoA binding protein is demonstrated. The sequence of the first six N‐terminal residues was obtained by derivatization of the A‐chain of insulin.</description><subject>Amino Acid Sequence</subject><subject>Mass Spectrometry - methods</subject><subject>Molecular Sequence Data</subject><subject>Peptides - genetics</subject><subject>Sequence Alignment - methods</subject><issn>1052-9306</issn><issn>0887-6134</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF1PwjAUhhujQUVvvTPZD7DYD7q1l0oUDQgxYEy8abrtLEw3NtcS3b-3OAJX5-M9z5ucF6ErSgaUEHYbl3ZAGSFU-ZEfoTNKVIiVlPJ42wuGFSfhKTq39tMfSH_VQz3GpJBMnKHVAr43sE4gSMFBU-Zr4_JqHVRZMMMmAdcWxkGKZzdzXHsd3KrbBDXULk_BBnEb1IWxpfEWtmrqf7401ga2hsQ1lYea9gKdZKawcLmrffT2-LAcPeHpfPw8upvinLMhxypTkCRcxEYZIRgJY0YzyihJVJimAnwjIhJnkRQqjZKMDaUIaQZc0KFhDHgfXXe-9SYuIdV1k5emafXuY6-rTv_JC2j3MiV6G6f2cepDnPr-ZXGYPIs7NrcOfvesab50GPFI6PfZWL9OFV1ORh96wv8A5Lt5zg</recordid><startdate>199010</startdate><enddate>199010</enddate><creator>Talbo, Gert</creator><creator>Roepstorff, Peter</creator><general>John Wiley &amp; Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199010</creationdate><title>Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry</title><author>Talbo, Gert ; Roepstorff, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3243-9f9ecc35ba9a55206b21f1210c96dd5e10c570bf7859d7cf248561fe3514a22e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Mass Spectrometry - methods</topic><topic>Molecular Sequence Data</topic><topic>Peptides - genetics</topic><topic>Sequence Alignment - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Talbo, Gert</creatorcontrib><creatorcontrib>Roepstorff, Peter</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Biological Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Talbo, Gert</au><au>Roepstorff, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry</atitle><jtitle>Biological Mass Spectrometry</jtitle><addtitle>Biol. Mass Spectrom</addtitle><date>1990-10</date><risdate>1990</risdate><volume>19</volume><issue>10</issue><spage>589</spage><epage>596</epage><pages>589-596</pages><issn>1052-9306</issn><issn>0887-6134</issn><eissn>1096-9888</eissn><abstract>The plasma desorption mass spectra of N‐acetylated‐N,O‐permethylated peptides contain sufficient fragment ions to allow partial or complete sequence determination. By optimization of the derivatization procedure and an inclusion of a purification step by high‐performance liquid chromatrography (HPLC) overall sensitivities on the high picomole level are obtained. By using limiting derivatization conditions the fully derivatized peptide is easily selected from the HPLC separation. Mainly N‐terminal sequence ions are observed, facilitating sequence determination of naturally N‐blocked peptides. Complete sequence determination of naturally formylated gramicidin A containing 15 amino acid residues and the naturally N‐acetylated N‐terminal heptapeptide from an acyl‐CoA binding protein is demonstrated. The sequence of the first six N‐terminal residues was obtained by derivatization of the A‐chain of insulin.</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><pmid>2285825</pmid><doi>10.1002/bms.1200191003</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1052-9306
ispartof Biological Mass Spectrometry, 1990-10, Vol.19 (10), p.589-596
issn 1052-9306
0887-6134
1096-9888
language eng
recordid cdi_wiley_primary_10_1002_bms_1200191003_BMS1200191003
source MEDLINE; Wiley Online Library Journals; Alma/SFX Local Collection
subjects Amino Acid Sequence
Mass Spectrometry - methods
Molecular Sequence Data
Peptides - genetics
Sequence Alignment - methods
title Sequence determination of N-acetylated-N,O-permethylated peptides by plasma desorption mass spectrometry
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T15%3A28%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-istex_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequence%20determination%20of%20N-acetylated-N,O-permethylated%20peptides%20by%20plasma%20desorption%20mass%20spectrometry&rft.jtitle=Biological%20Mass%20Spectrometry&rft.au=Talbo,%20Gert&rft.date=1990-10&rft.volume=19&rft.issue=10&rft.spage=589&rft.epage=596&rft.pages=589-596&rft.issn=1052-9306&rft.eissn=1096-9888&rft_id=info:doi/10.1002/bms.1200191003&rft_dat=%3Cistex_pubme%3Eark_67375_WNG_QL91TKCZ_K%3C/istex_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/2285825&rfr_iscdi=true