Accurate, sensitive determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma, urine samples
Quantitative determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma and urine with high accuracy and precision provides significant information to monitor the underlying etiology of several diseases. In this regard, liquid chromatography‐mass spectrometry is a good choice o...
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| Veröffentlicht in: | Biomedical chromatography 2020-12, Vol.34 (12), p.e4951-n/a, Article 4951 |
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| creator | Er, Elif Ozturk Sahin, Ayse Bakirdere, Sezgin |
| description | Quantitative determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma and urine with high accuracy and precision provides significant information to monitor the underlying etiology of several diseases. In this regard, liquid chromatography‐mass spectrometry is a good choice owing to its great selectivity and sensitivity. Additionally, the hybrid quadrupole–time of flight–mass spectrometer systems provides easy identification of target compounds with superior mass measurements. In this study, an analytical method has been developed for simple, accurate and simultaneous determination of linoleic acid, arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid in a short chromatographic analysis period. The developed method is suitable for the quantitative detection of these four compounds with detection limits ranging between 1.1–3.0 ng ml−1 and its applicability was assessed in human urine and plasma samples. As a result, acceptable accuracy (between 83 and 111%) and good precision ( |
| doi_str_mv | 10.1002/bmc.4951 |
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In this regard, liquid chromatography‐mass spectrometry is a good choice owing to its great selectivity and sensitivity. Additionally, the hybrid quadrupole–time of flight–mass spectrometer systems provides easy identification of target compounds with superior mass measurements. In this study, an analytical method has been developed for simple, accurate and simultaneous determination of linoleic acid, arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid in a short chromatographic analysis period. The developed method is suitable for the quantitative detection of these four compounds with detection limits ranging between 1.1–3.0 ng ml−1 and its applicability was assessed in human urine and plasma samples. 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In this regard, liquid chromatography‐mass spectrometry is a good choice owing to its great selectivity and sensitivity. Additionally, the hybrid quadrupole–time of flight–mass spectrometer systems provides easy identification of target compounds with superior mass measurements. In this study, an analytical method has been developed for simple, accurate and simultaneous determination of linoleic acid, arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid in a short chromatographic analysis period. The developed method is suitable for the quantitative detection of these four compounds with detection limits ranging between 1.1–3.0 ng ml−1 and its applicability was assessed in human urine and plasma samples. As a result, acceptable accuracy (between 83 and 111%) and good precision (<6%) were obtained for target compounds using matrix matching calibration strategy.</description><subject>Biochemical Research Methods</subject><subject>Biochemistry & Molecular Biology</subject><subject>Chemistry</subject><subject>Chemistry, Analytical</subject><subject>Chromatography, Liquid - methods</subject><subject>Fatty Acids, Omega-3 - blood</subject><subject>Fatty Acids, Omega-3 - urine</subject><subject>Fatty Acids, Omega-6 - blood</subject><subject>Fatty Acids, Omega-6 - urine</subject><subject>human plasma</subject><subject>human urine</subject><subject>Humans</subject><subject>Life Sciences & Biomedicine</subject><subject>Limit of Detection</subject><subject>Linear Models</subject><subject>liquid chromatography–mass spectrometry</subject><subject>matrix‐matching calibration strategy</subject><subject>Pharmacology & Pharmacy</subject><subject>Physical Sciences</subject><subject>polyunsaturated fatty acids</subject><subject>Reproducibility of Results</subject><subject>Science & Technology</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQhy1ERbcFiSdAPiLRFP9JnPhYIihIrbjAOZrYEzCK7RA7rfbGI_CMPAnZ7bKckJiLNdI3P818JuQ5Z5ecMfG69-ay1BV_RDacaV2whvHHZMOE0oVsan1KzlL6xhjTStRPyKkUNVMlkxtyf2XMMkPGC5owJJfdHVKLGWfvAmQXA40DjR6_wK8fPxWFYI-dpFMct0tIkPcRlg6Q85aCcTZRF-jXxUOg0wjJwwVdZheQJvDTiOkpORlgTPjs8J6Tz-_efmrfFzcfrz-0VzeFEY3gRaWNkAb6SvVDowCB86oRqKwebGnK9fheAEfLhdQwlI2yomcNRyOBsbqu5Tl5-ZA7zfH7gil33iWD4wgB45I6UYpaMsWV-IuaOaY049BNs_MwbzvOup3mbtXc7TSv6ItD6tJ7tEfwj9cVaB6Ae-zjkIzDYPCIrR9RVYqLku2Kty7vTbdxCXkdffX_oytdHGg34vafG3dvbtv95r8BZNipsA</recordid><startdate>202012</startdate><enddate>202012</enddate><creator>Er, Elif Ozturk</creator><creator>Sahin, Ayse</creator><creator>Bakirdere, Sezgin</creator><general>Wiley</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4877-2662</orcidid><orcidid>https://orcid.org/0000-0001-9746-3682</orcidid></search><sort><creationdate>202012</creationdate><title>Accurate, sensitive determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma, urine samples</title><author>Er, Elif Ozturk ; Sahin, Ayse ; Bakirdere, Sezgin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2821-59c23cab56bf86aea11582e6d9fd4c4100b2a1ed1239af486d2b081ec3a007773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biochemical Research Methods</topic><topic>Biochemistry & Molecular Biology</topic><topic>Chemistry</topic><topic>Chemistry, Analytical</topic><topic>Chromatography, Liquid - methods</topic><topic>Fatty Acids, Omega-3 - blood</topic><topic>Fatty Acids, Omega-3 - urine</topic><topic>Fatty Acids, Omega-6 - blood</topic><topic>Fatty Acids, Omega-6 - urine</topic><topic>human plasma</topic><topic>human urine</topic><topic>Humans</topic><topic>Life Sciences & Biomedicine</topic><topic>Limit of Detection</topic><topic>Linear Models</topic><topic>liquid chromatography–mass spectrometry</topic><topic>matrix‐matching calibration strategy</topic><topic>Pharmacology & Pharmacy</topic><topic>Physical Sciences</topic><topic>polyunsaturated fatty acids</topic><topic>Reproducibility of Results</topic><topic>Science & Technology</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Er, Elif Ozturk</creatorcontrib><creatorcontrib>Sahin, Ayse</creatorcontrib><creatorcontrib>Bakirdere, Sezgin</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Er, Elif Ozturk</au><au>Sahin, Ayse</au><au>Bakirdere, Sezgin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accurate, sensitive determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma, urine samples</atitle><jtitle>Biomedical chromatography</jtitle><stitle>BIOMED CHROMATOGR</stitle><addtitle>Biomed Chromatogr</addtitle><date>2020-12</date><risdate>2020</risdate><volume>34</volume><issue>12</issue><spage>e4951</spage><epage>n/a</epage><pages>e4951-n/a</pages><artnum>4951</artnum><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>Quantitative determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma and urine with high accuracy and precision provides significant information to monitor the underlying etiology of several diseases. In this regard, liquid chromatography‐mass spectrometry is a good choice owing to its great selectivity and sensitivity. Additionally, the hybrid quadrupole–time of flight–mass spectrometer systems provides easy identification of target compounds with superior mass measurements. In this study, an analytical method has been developed for simple, accurate and simultaneous determination of linoleic acid, arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid in a short chromatographic analysis period. The developed method is suitable for the quantitative detection of these four compounds with detection limits ranging between 1.1–3.0 ng ml−1 and its applicability was assessed in human urine and plasma samples. 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| subjects | Biochemical Research Methods Biochemistry & Molecular Biology Chemistry Chemistry, Analytical Chromatography, Liquid - methods Fatty Acids, Omega-3 - blood Fatty Acids, Omega-3 - urine Fatty Acids, Omega-6 - blood Fatty Acids, Omega-6 - urine human plasma human urine Humans Life Sciences & Biomedicine Limit of Detection Linear Models liquid chromatography–mass spectrometry matrix‐matching calibration strategy Pharmacology & Pharmacy Physical Sciences polyunsaturated fatty acids Reproducibility of Results Science & Technology Tandem Mass Spectrometry - methods |
| title | Accurate, sensitive determination of omega‐6 and omega‐3 polyunsaturated fatty acids in human plasma, urine samples |
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