Radioligand Binding Analysis as a Tool for Quality Control of GPCR Production for Structural Characterization: Adenosine A2aR as a Template for Study

Functional characterization of G protein–coupled receptors is essential to ascertain the suitability of a protein target for downstream studies and to help develop optimal expression and isolation procedures. Radioligand binding analysis is a well‐established technique, which allows direct measureme...

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Veröffentlicht in:	Current Protocols in Protein Science 2012-02, Vol.29 (1), p.29.3.1-29.3.22
Hauptverfasser:	Singh, Shweta, Zhang, Minghao, Bertheleme, Nicolas, Kara, Elodie, Strange, Philip G., Byrne, Bernadette
Format:	Artikel
Sprache:	eng
Schlagworte:	adenosine A2aR competitive radioligand binding assay dissociation constant G protein–coupled receptor saturation radioligand binding assay specific activity
Online-Zugang:	Volltext
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