Studying RNA Using Single Molecule Fluorescence Resonance Energy Transfer

The intrinsic structure of RNA and in particular the dynamics of the same are of paramount importance for RNA function. Recent work, e.g., on riboswitches and ribosomes, shows the vast complexity of these dynamics. Consequently the interest in biochemical and biophysical methods to investigate these...

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Bibliographische Detailangaben
Hauptverfasser:	Spenkuch, Felix, Domingo, Olwen, Hinze, Gerald, Basché, Thomas, Helm, Mark
Format:	Buchkapitel
Sprache:	eng
Schlagworte:	acceptor fluorophore antifading agents bulk FRET measurements donor fluorophore dye labeling of RNA by click chemistry emission spectra excitation spectra Fluorescence resonance energy transfer (FRET) FRET dye pairs Förster radius immobilization of RNA by biotin‐streptavidin interaction measurement chamber design PEG‐coating of surfaces photobleaching RNA structure, conformation, dynamics, and structural homogeneity single molecule FRET (smFRET)
Online-Zugang:	Volltext
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