Morphogenesis of African swine fever virus in monkey kidney cells after reversible inhibition of replication by cycloheximide

The late cytoplasmic phases of African swine fever virus (ASFV) morphogensis in monkey kidney cells have been studied by transmission electron microscopy, focusing attention on the synthesis of viral envelopes. Morphogenesis was studied after reversible cycloheximide blockage of monkey kidney cells infected with ASFV. ASFV appears to synthesize its external and internal envelopes within the cellular cytoplasm, at the same time as the capsid is formed, with intracellular and extracellular virions showing similar structure and polypeptide composition.